TY - JOUR T1 -木材烟雾对上皮细胞系和人气道细胞的影响JF -欧洲呼吸杂志JO - Eur Respir J DO - 10.1183/13993003.congress-2019。PA5448六世- 54 - 5 63 SP - PA5448 AU -汉森,阿尔瓦AU - Rankin,格雷格•AU - Uski Oskari盟——Sehlstedt玛丽亚盟——彼此,詹妮非盟- Pourazar Jamshid AU -鲍曼,christof盟——林格伦,罗伯特•AU -加西亚洛佩兹Natxo盟——Behndig Annelie盟,显得过于安德斯盟),托马斯•AU - Muala Ala Y1 - 2019/09/28 UR - //www.qdcxjkg.com/content/54/suppl_63/PA5448.abstract N2 -背景:接触生物质燃烧产生的排放物会导致呼吸道疾病，包括阻塞性呼吸道疾病、急性下呼吸道感染和结核病。潜在的机制和影响还没有被很好地理解。目的:确定暴露于不完全燃烧产生的木材烟雾是否会引起气道细胞和上皮细胞系的DNA损伤。方法:根据双盲随机交叉设计，14名健康受试者在受控室内两次暴露于过滤空气和瑞典桦木炉产生的煤烟(PM1 ~ 450 μg/m3)。受试者交替运动(VE=20 L/min/m2)和休息，间隔15分钟，持续2小时。暴露后6小时进行支气管镜检查，取样支气管冲洗(BW)和支气管肺泡灌洗(BAL)。此外，肺泡II型细胞株A549和支气管上皮细胞株BEAS-2B暴露于四种不同剂量的木烟颗粒中。用Comet法检测DNA损伤，并定量橄榄尾矩(OTM)。Results: Eosinophil numbers was increased in BAL after exposure to wood smoke, with no changes in other cells. Moreover, there was a dose-dependent increase in DNA damage in vitro when comparing PM concentrations above 75µg ml-1 to air.Conclusion: Short term exposure to sooty wood smoke induced a bronchoalveolar eosinophilic insult. This may link with the development and worsening of asthma after chronic exposure to wood smoke. Moreover, the observed DNA damage in vitro may explain the previously shown reduction in airway cells, which in turn may causally affect cellular immunity and increase the incidence of respiratory infectionFootnotesCite this article as: European Respiratory Journal 2019; 54: Suppl. 63, PA5448.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only). ER -