TY -的T1 Whole-transcriptome测序揭示加剧炎症和缺陷宿主防御反应在慢性鼻窦炎鼻息肉JF -欧洲呼吸杂志》乔和J - 10.1183/13993003.00732 -2019欧元六世- 54 - 5 SP - 1900732 AU -彭,杨AU -子,Xiao-Xue盟——田,腾飞AU -李,Bernett AU - Lum, Josephine AU - Tang, See Aik AU - Tan, Kai Sen AU - Qiu, qian hui AU - Ye, Jing AU - Shi, Li AU - Guan, Wei-Jie AU - Andiappan, Anand Kumar AU - Wang，慢性鼻-鼻窦炎伴鼻息肉(CRSwNP)的发病机制尚不清楚。我们进行了全基因组基因表达分析，以确定与CRSwNP相关的通路和候选基因集。方法对CRSwNP患者的42对息肉组织(CRSwNP- np)和33对非息肉性下鼻甲组织(CRSwNP- it)和28对非息肉性下鼻甲组织(CS-IT)进行全转录组RNA测序。我们分析了差异表达基因(DEGs)和在功能通路中富集的基因集。结果主成分分析显示纤毛功能和免疫调节是区分CRSwNP患者与对照组的两个主要基因本体(GO)类别。我们检测到CRSwNP-NP与CS-IT之间以及CRSwNP-NP与CRSwNP-IT之间分别存在6182和1592个deg。异位状态对不同组织中的基因表达没有重大影响。GO分析这些DEGs与CRSwNP发病过程中的细胞外基质(ECM)分解、o -聚糖加工、血管生成和宿主病毒反应有关。 Ingenuity Pathway Analysis identified significant enrichment of type 1 interferon signalling and axonal guidance canonical pathways, angiogenesis, and collagen and fibrotic changes in CRSwNP (CRSwNP-NP and CRSwNP-IT) tissues compared with CS-IT. Finally, gene set enrichment analysis implicated sets of genes co-regulated in processes associated with inflammatory response and aberrant cell differentiation in polyp formation.Conclusions Gene signatures involved in defective host defences (including cilia dysfunction and immune dysregulation), inflammation and abnormal metabolism of ECM are implicated in CRSwNP. Functional validation of these gene expression patterns will open opportunities for CRSwNP therapeutic interventions such as biologics and immunomodulators.Whole-transcriptome sequencing shows gene signatures associated with interferon signalling response and host defence are implicated in chronic rhinosinusitis with nasal polyps, opening up new opportunities for therapeutic interventions http://bit.ly/2KyF3dT ER -