TY -的T1 -术供体特异性表型anti-HLA抗体的慢性肺部同种异体移植物功能障碍JF -欧洲呼吸杂志》乔和J - 10.1183/13993003.00847 -2019欧元六世- 54 - 5 SP - 1900847 AU Sacreas Annelore AU -陶品,jean - luc盟——Emonds marie - paule AU -丹尼尔斯,利斯盟- Van Raemdonck,德克·e . AU - Vos,罗宾盟——Verleden基尔特•m . AU - Vanaudenaerde巴特·m . AU -面粉糊,安东尼盟——Verleden Stijn e . A2 -循环抗人白细胞抗原(HLA)血清供者特异性抗体(sDSAs)增加慢性异体肺功能障碍(CLAD)和死亡率的风险。血清学和病理/临床结果之间的差异是常见的。因此，我们的目的是评估包覆的外植组织中组织结合移植物DSAs (gDSAs)的存在情况，并与sDSAs进行比较。方法组织核心,从外植体获得肺未使用的捐助者(n = 10)和闭塞性细支气管炎综合征患者(BOS;和限制性同种异体移植综合征(RAS;n=18)，抗体洗脱前用微计算机断层扫描。ELISA法测定总IgG水平。使用Luminex单抗原珠鉴定hla I类和II类IgG gDSAs，并与血清中发现的DSAs进行比较。结果总体而言，RAS洗脱液的平均荧光强度高于BOS和对照组(p<0.0001)。在BOS中，2例为sDSA+/gDSA+， 2例为sDSA−/gDSA+。 In RAS, four patients were sDSA+/gDSA+, one patient was sDSA+/gDSA− and five patients were sDSA−/gDSA+. Serum and graft results combined, DSAs were more prevalent in RAS compared with BOS (56% versus 22%; p=0.04). There was spatial variability in gDSA detection in one BOS patient and three RAS patients, who were all sDSA−. Total graft IgG levels were higher in RAS than BOS (p<0.0001) and in gDSA+ versus gDSA− (p=0.0008), but not in sDSA+ versus sDSA− (p=0.33). In RAS, total IgG levels correlated with fibrosis (r= −0.39; p=0.02).Conclusions This study underlines the potential of gDSA assessment as complementary information to sDSA findings. The relevance and applications of gDSAs need further investigation.Discrepancies between serological and pathological/clinical findings in DSAs after lung transplantation are common. This article provides evidence that graft DSA assessment yields complementary information for lung transplant patients. http://bit.ly/2GIkae1 ER -