%0 Journal Article %A Knabe, Lucie %A Petit, Aurélie %A Vernisse, Charlotte %A Charriot, Jérémy %A Pugnière, Martine %A Henriquet, Corinne %A Sasorith, Souphatta %A Molinari, Nicolas %A Chanez, Pascal %A Berthet, Jean-Philippe %A Suehs, Carey %A Vachier, Isabelle %A Ahmed, Engi %A Bourdin，Arnaud %T CCSP平衡气道上皮驱动的中性粒细胞趋化性。这可能通过直接中和人类的IL8而发生。http://bit.ly/2UGJlGWClub细胞分泌蛋白(CCSP)敲除小鼠表现出气道中性粒细胞增多，如在慢性阻塞性肺疾病(COPD)中发现的那样。因此，我们研究了重组人CCSP (rhCCSP)治疗COPD气道上皮是否能抑制夸大的气道中性粒细胞增多。对照组、吸烟者和暴露于香烟烟雾提取物(CSE)的COPD气液界面(ALI)培养物分别经rhCCSP处理和不经rhCCSP处理。上清液的趋化特性用Dunn chambers进行评估。中性粒细胞趋化性沿着重组人白细胞介素8 (rhhil8)梯度(有和没有rhCCSP)也被确定。rhccsp - rhhil8相互作用通过共免疫沉淀、Biacore表面等离子体共振(SPR)和硅模型来检测。评估慢性阻塞性肺病患者诱导痰上清中CCSP/IL8浓度比与肺活检中中性粒细胞气道浸润的关系。ce处理的ALI培养物的中性粒细胞趋化活性随着IL8浓度的增加而增加，补充rhCCSP后恢复正常。rhCCSP降低了rhhil8诱导的中性粒细胞趋化。 rhCCSP and rhIL8 co-immunoprecipitated. SPR confirmed this in vitro interaction (equilibrium dissociation constant=8 µM). In silico modelling indicated that this interaction was highly likely. CCSP/IL8 ratios in induced sputum correlated well with the level of small airway neutrophilic infiltration (r2=0.746, p<0.001).CCSP is a biologically relevant counter-balancer of neutrophil chemotactic activity. These different approaches used in this study suggest that, among the possible mechanisms involved, CCSP may directly neutralise IL8. %U //www.qdcxjkg.com/content/erj/54/1/1802408.full.pdf