TY -的T1 -针对TMEM16A扭转vasoconstriction and remodelling in idiopathic pulmonary arterial hypertension JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.00965-2018 VL - 53 IS - 6 SP - 1800965 AU - Papp, Rita AU - Nagaraj, Chandran AU - Zabini, Diana AU - Nagy, Bence M. AU - Lengyel, Miklós AU - Skofic Maurer, Davor AU - Sharma, Neha AU - Egemnazarov, Bakytbek AU - Kovacs, Gabor AU - Kwapiszewska, Grazyna AU - Marsh, Leigh M. AU - Hrzenjak, Andelko AU - Höfler, Gerald AU - Didiasova, Miroslava AU - Wygrecka, Malgorzata AU - Sievers, Laura K. AU - Szucs, Peter AU - Enyedi, Péter AU - Ghanim, Bahil AU - Klepetko, Walter AU - Olschewski, Horst AU - Olschewski, Andrea Y1 - 2019/06/01 UR - //www.qdcxjkg.com/content/53/6/1800965.abstract N2 - Our systematic analysis of anion channels and transporters in idiopathic pulmonary arterial hypertension (IPAH) showed marked upregulation of the Cl− channel TMEM16A gene. We hypothesised that TMEM16A overexpression might represent a novel vicious circle in the molecular pathways causing pulmonary arterial hypertension (PAH).We investigated healthy donor lungs (n=40) and recipient lungs with IPAH (n=38) for the expression of anion channel and transporter genes in small pulmonary arteries and pulmonary artery smooth muscle cells (PASMCs).In IPAH, TMEM16A was strongly upregulated and patch-clamp recordings confirmed an increased Cl− current in PASMCs (n=9–10). These cells were depolarised and could be repolarised by TMEM16A inhibitors or knock-down experiments (n=6–10). Inhibition/knock-down of TMEM16A reduced the proliferation of IPAH-PASMCs (n=6). Conversely, overexpression of TMEM16A in healthy donor PASMCs produced an IPAH-like phenotype. Chronic application of benzbromarone in two independent animal models significantly decreased right ventricular pressure and reversed remodelling of established pulmonary hypertension.Our findings suggest that increased TMEM16A expression and activity comprise an important pathologic mechanism underlying the vasoconstriction and remodelling of pulmonary arteries in PAH. Inhibition of TMEM16A represents a novel therapeutic approach to reverse remodelling in PAH.TMEM16A plays a central role in the pathological mechanisms underlying the depolarisation, vasoconstriction and proliferation of PASMCs, contributing to the increased pulmonary vascular resistance in PAH, thus providing a novel target for PAH therapy http://ow.ly/3Rs330o3CUy ER -