TY - T1的肥大细胞类胰蛋白酶诱导alarmin释放和支气管上皮细胞分泌的促炎细胞因子JF -欧洲呼吸杂志》乔和J - 10.1183/13993003.国会2018欧元。PA5002六世- 52 - 5 62 SP - PA5002 AU - Ramu矿盟Sangeetha列夫Bjermer AU -乔纳斯Erjefalt盟Lena ul AU -塞西莉亚Andersson Y1 - 2018/09/15 UR - //www.qdcxjkg.com/content/52/suppl_62/PA5002.abstract N2 -背景:肥大细胞(MC)密度增加哮喘的气道上皮细胞是一个重要的标志。在上皮细胞内,MCs是理想的位置,可以在有害物质和反应产生免疫反应。然而,所知甚少的直接影响MC介质上皮。摘要目的:探讨密度和表型的MCs在支气管哮喘患者的活检和MC中介类胰蛋白酶的作用在支气管上皮细胞(bec)。方法:上皮内MCs在哮喘患者支气管活检从特征和控制使用免疫组织化学类胰蛋白酶(MCT)和类胰蛋白酶/ chymase (MCTC)。研究MC中介类胰蛋白酶的作用在bec, BEAS-2B和16 hbe细胞系服用类胰蛋白酶(0.1 - 1海里)1、3、6和24小时。文化上层清液和细胞溶解产物进行评估alarmin和炎性细胞因子释放和紧密连接蛋白的表达。结果:Tryptase-positive mct,但不是MCTCs,增加支气管上皮细胞中抑制哮喘患者与健康对照组相比。此外,我们的数据表明,类胰蛋白酶诱导alarmin ATP的释放bec 1 h后刺激。 Also, tryptase induced inflammatory cytokine CXCL8 gene and protein release from BECs at 6 and 24h, respectively. Additionally, tryptase induced a downregulation of the tight junction proteins ZO-1 and E-cadherin mRNA.Conclusion: Our results suggest that tryptase released from MCs may play a direct role in inducing an inflammatory response and a damaged barrier function in BECs which may have an important role in the progression of asthma pathology.FootnotesCite this article as: European Respiratory Journal 2018 52: Suppl. 62, PA5002.This is an ERS International Congress abstract. No full-text version is available. Further material to accompany this abstract may be available at www.ers-education.org (ERS member access only). ER -