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\Bp^o=53[^]_%twlHivB q+p]9 …w޳ŵ4洨liyŒO{RJ5מCOvx唼@}5\|MUJ_pVmR)UBB7Gj 3v 2,'aяu"½Z]kj٤Eڲ+h8>{'R ›j8/$LKQ])2 Wb1ֵ-V#DV uq/x5\1qG(# eru[j8aV:Wz*;eՐ7$uwlHjv,UBBS_Ě oyGؒ CptrT\s-ݘ\Upeq8מ?g\8J/#@pV 61?SybWy1.pE::kc[GRo&rՆz 5\ 61e(R =ߵ-U%$ZV \7">JW?qiSOGӦu;0bB{ӼĴSuh,Ty+& 2{2 W%aaKZض+ -a:+R-Bht1mIqbZ06UVx.{%ӂV;(˅RWp>+\IRǔav#~ m8  BR!᠍Dwڞ#>oҶk%ekez4!̫`( 1hMB (i8R^V*9.0":+BV{X֧J?p9vuN T1Wprv&SvKʞ{kN>%kc[4e.5DVt+:vbz~wZ}RàŶ‘<}v#31n/m(֫K\BlM= rQe[2}= $64J#Hjv,UDȿBZ&pRL'aяN6n+ݧ<3"dc.9[d\b´(dL /\BBMT%fef[H,MզciwmhlIjv,UD5_!@ qH8h+_q~wQ-m:.e- (a /ӐnQ6ùpZd~RY._|EY:G>8(@)A3kc۪4DR(d4":+="nݻ.r(_W~Ky /7m1p~{-J>IzM2]BBM)jiir+BvȮ mItK{cýh_h1R,s!V| jUZ'>7zo>&gCHsIS1Z\WGwmxl[fǍc¿BH!^pb.p/,zo{y"H.`QI=wz]5\|Tü(5JC3̲K]1J+rG2_p uPW$1{h3h`gHs@^E@!co.`Ol\|_(!iNϗ j3E]&kYV˲L6fl[V]h8mX;(+fvobVsԖ] \kN[Ha;Zm5\>_"z嫚< ]ì$=h=ٵc;+2 `G=@(n|"_p Q_Tu^S[mgVkx_R]=疪n5b$Q}N("0JO[䄌}}o/ZmDŽfvkNj[σe]?Cf?\gX;(<-dX mg^,rid?W -6DYziu9OWG/+_F%Qܛ>nt-6kOAL׍}\2ܱ]ٱ|WTOb}uM5O;'Gz1u4l+^鵅dO}Hgc-7-kw(q]=f?AxXEuPxS-GҺ 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O$9i߁#(-rhi1e*7_&ÛTiu:#|Ҁ<-xt ^Dy,S176ֵ=oׇRB8x3ݨivu~ib) RcL7WjE(y^W`ő Ax ͝\_ՑGج6XMq0j+pȎ08] B /t@T #IBEi|-,jSUi݊hꈽf K~БPU`.fF۠/(X<־ݥ- zv\ zv w=OwQN2:Z4$O)lESrIkGfhJ. ִrT9.0%dTߦ^-5NӴ/.Hw%;6xݬ/ZQ NRC*d*',dYPd`A 5g"IW%uuT,Rʲ-O泝S˜@)-go!ÙdSsJ֕m SѴPOQ9f(e/m.@*UAyܻuo{miivrM`v),&]s6o_Xr6m2_l:#r)"5L$4H '1k"ה5;ɜX|>P7ry M)%ƪD܍3F9=L4xt1ÁL8d]JUbV%&24H1Oe=#0Ҕ߽zju A, EWV}±bF endstream endobj 5098 0 obj << /ExtGState << /GS1 5103 0 R >> /ColorSpace << /Cs6 5104 0 R >> /XObject << /Im77 5139 0 R /Im76 5140 0 R /Im75 5141 0 R /Im78 5142 0 R >> /Font << /TT2 5126 0 R /TT1 5127 0 R >> /ProcSet [/PDF /Text /ImageB] >> endobj 5141 0 obj << /DecodeParms << /Columns 112 /K -1 >> /ImageMask true /Width 112 /BitsPerComponent 1 /Height 1170 /Filter /CCITTFaxDecode /Subtype /Image /Length 810 /Type /XObject >> stream j|,7PJuKUuJ/KU<MW_)Š\. p\. p\.\ \\ @ApApp  !`XXX,,, °Q… (( P@B  > p@@ \ ]]^ ^ ׃~~`0|01xx0p` 8888ppppp;@ڕ'*XBK ("+*!d }!Dy `XXV )@nIt>-R_KWvo mXa`X0VAn8paAgzx w4(@„ UV@ rpt='v nu_Zֵv ..Nj  A ( PAB PA  P@(  T a`X,, `Q!@zw+`p`* *TJ/KU~<C,=pUJT*Tj*ꖫzݽy @kø89Xo4__|,7A<<<vCS|k!dbHd<7ÇJ@AP-(&a;1 endstream endobj 5140 0 obj << /DecodeParms << /Columns 56 /K -1 >> /ImageMask true /Width 56 /BitsPerComponent 1 /Height 1170 /Filter /CCITTFaxDecode /Subtype /Image /Length 504 /Type /XObject >> stream MC?ڵp?5 GkPNaaGupXN  `MVxx< ASȁRYx<<<s0lVj x<x&D {J}kXZXH pA᧰OiޟO/n Xk c{Zjի Pƒ!Oజ `J  a`XX, 3PMC?ɨg^? 8XX, 0ԚmI*aaaa`X,,Ar,&<@ endstream endobj 5139 0 obj << /DecodeParms << /Columns 112 /K -1 >> /ImageMask true /Width 112 /BitsPerComponent 1 /Height 1110 /Filter /CCITTFaxDecode /Subtype /Image /Length 791 /Type /XObject >> stream j; #U_ k ڏ@W_#zCð;ða0ap0 ;pÆ 8apÆ7 8av0l8p8pÇ9_w; (e`5P8At *uC0R  I:O]:}W_Uԕ kNv ɠmJP,!@z%y AXDa2" {> /ImageMask true /Width 56 /BitsPerComponent 1 /Height 1110 /Filter /CCITTFaxDecode /Subtype /Image /Length 465 /Type /XObject >> stream PPU<=}aGU{o꾺_kQjy*A x<Ãf @j@"xx5j @nJZ__ZֵaaaGU8NMd{ /x M}^]o박`;^׵VZ (2 N `XX, J`X, `QMC?5 3MC?B'a`XX,DDRha&`Y` T endstream endobj 5096 0 obj << /Filter /FlateDecode /Length 597 >> stream hlSM0W錿-rEnH{@J, E7Of%[c= fT9k~>W' lio@>ur1RɻOb/7;x(7yͳBG(CqIrvϨ/PQ5E }]*SrH"FSeX^m讳Fj3J endstream endobj 5095 0 obj << /ExtGState << /GS1 5103 0 R >> /ColorSpace << /Cs6 5104 0 R >> /XObject << /Im74 5143 0 R >> /Font << /TT3 5144 0 R /TT2 5126 0 R /TT1 5127 0 R >> /ProcSet [/PDF /Text /ImageC] >> endobj 5143 0 obj << /ColorSpace 5104 0 R /Subtype /Image /Filter /DCTDecode /Length 57117 /Width 1054 /Type /XObject /BitsPerComponent 8 /Height 554 >> stream Adobed     $$''$$53335;;;;;;;;;;  %% ## ((%%((22022;;;;;;;;;;*"? 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42 0 R /K [4342 0 R 4343 0 R 4344 0 R 4345 0 R 4346 0 R 4347 0 R 4348 0 R] /S /P >> endobj 5254 0 obj << /Pg 5058 0 R /P 42 0 R /K [4349 0 R 4350 0 R 4351 0 R 4352 0 R 4353 0 R 4354 0 R 4355 0 R 4356 0 R 4357 0 R 4358 0 R 4359 0 R] /S /P >> endobj 5255 0 obj << /Pg 5058 0 R /P 42 0 R /K 4360 0 R /S /P >> endobj 5256 0 obj << /Pg 5058 0 R /P 42 0 R /K [4361 0 R 4362 0 R] /S /P >> endobj 5257 0 obj << /Pg 5058 0 R /P 42 0 R /K [4363 0 R 4364 0 R 4365 0 R 4366 0 R 4367 0 R 4368 0 R 4369 0 R 4370 0 R 4371 0 R 4372 0 R 4373 0 R] /S /P >> endobj 5258 0 obj << /Pg 5058 0 R /P 42 0 R /K 4374 0 R /S /P >> endobj 5259 0 obj << /Pg 5058 0 R /P 42 0 R /K [4375 0 R 4376 0 R 4377 0 R 4378 0 R] /S /P >> endobj 5260 0 obj << /Pg 5058 0 R /P 42 0 R /K [4379 0 R 4380 0 R 4381 0 R 4382 0 R 4383 0 R 4384 0 R 4385 0 R 4386 0 R 4387 0 R 4388 0 R 4389 0 R 4390 0 R 4391 0 R 4392 0 R 4393 0 R 4394 0 R] /S /P >> endobj 5261 0 obj << /Pg 5058 0 R /P 42 0 R /K 4395 0 R /S /P >> endobj 5262 0 obj << /Pg 5058 0 R /P 42 0 R /K [4396 0 R 4397 0 R] /S /P >> endobj 5263 0 obj << /Pg 5058 0 R /P 42 0 R /K [4398 0 R 4399 0 R 4400 0 R 4401 0 R 4402 0 R 4403 0 R 4404 0 R 4405 0 R 4406 0 R 4407 0 R 4408 0 R 4409 0 R 4410 0 R 4411 0 R 4412 0 R 4413 0 R 4414 0 R 4415 0 R] /S /P >> endobj 5264 0 obj << /Pg 5058 0 R /P 42 0 R /K 4416 0 R /S /P >> endobj 5265 0 obj << /Pg 5058 0 R /P 42 0 R /K [4417 0 R 4418 0 R 4419 0 R 4420 0 R] /S /P >> endobj 5266 0 obj << /Pg 5058 0 R /P 42 0 R /K [4421 0 R 4422 0 R 4423 0 R 4424 0 R 4425 0 R] /S /P >> endobj 5267 0 obj << /Pg 5059 0 R /P 42 0 R /K [4426 0 R 4427 0 R 4428 0 R 4429 0 R 4430 0 R 4431 0 R 4432 0 R 4433 0 R 4434 0 R 4435 0 R 4436 0 R 4437 0 R 4438 0 R 4439 0 R 4440 0 R 4441 0 R 4442 0 R 4443 0 R 4444 0 R 4445 0 R 4446 0 R 4447 0 R 4448 0 R 4449 0 R] /S /P >> endobj 5268 0 obj << /Pg 5059 0 R /P 42 0 R /K [4450 0 R 4451 0 R 4452 0 R 4453 0 R 4454 0 R 4455 0 R 4456 0 R 4457 0 R 4458 0 R 4459 0 R 4460 0 R 4461 0 R] /S /P >> endobj 5269 0 obj << /Pg 5059 0 R /P 42 0 R /K [4462 0 R 4463 0 R 4464 0 R 4465 0 R 4466 0 R 4467 0 R 4468 0 R 4469 0 R 4470 0 R 4471 0 R 4472 0 R] /S /P >> endobj 5270 0 obj << /Pg 5059 0 R /P 42 0 R /K 4473 0 R /S /P >> endobj 5271 0 obj << /Pg 5059 0 R /P 42 0 R /K [4474 0 R 4475 0 R] /S /P >> endobj 5272 0 obj << /Pg 5059 0 R /P 42 0 R /K [4476 0 R 4477 0 R 4478 0 R 4479 0 R 4480 0 R 4481 0 R 4482 0 R 4483 0 R 4484 0 R 4485 0 R 4486 0 R 4487 0 R 4488 0 R 4489 0 R 4490 0 R 4491 0 R 4492 0 R 4493 0 R 4494 0 R 4495 0 R 4496 0 R 4497 0 R 4498 0 R 4499 0 R 4500 0 R 4501 0 R 4502 0 R 4503 0 R 4504 0 R 4505 0 R 4506 0 R 4507 0 R 4508 0 R 4509 0 R 4510 0 R 4511 0 R 4512 0 R 4513 0 R 4514 0 R 4515 0 R 4516 0 R 4517 0 R 4518 0 R 4519 0 R 4520 0 R 4521 0 R 4522 0 R 4523 0 R 4524 0 R 4525 0 R 4526 0 R 4527 0 R 4528 0 R 4529 0 R 4530 0 R 4531 0 R 4532 0 R 4533 0 R 4534 0 R 4535 0 R 4536 0 R 4537 0 R 4538 0 R 4539 0 R 4540 0 R 4541 0 R 4542 0 R] /S /P >> endobj 5273 0 obj << /Pg 5059 0 R /P 42 0 R /K 4543 0 R /S /P >> endobj 5274 0 obj << /Pg 5059 0 R /P 42 0 R /K [4544 0 R 4545 0 R] /S /P >> endobj 5275 0 obj << /Pg 5059 0 R /P 42 0 R /K [4546 0 R 4547 0 R 4548 0 R 4549 0 R 4550 0 R 4551 0 R 4552 0 R 4553 0 R 4554 0 R 4555 0 R 4556 0 R 4557 0 R 4558 0 R 4559 0 R 4560 0 R 4561 0 R 4562 0 R 4563 0 R 4564 0 R] /S /P >> endobj 5276 0 obj << /Pg 5060 0 R /P 42 0 R /K [4565 0 R 4566 0 R 4567 0 R] /S /P >> endobj 5277 0 obj << /Pg 5060 0 R /P 42 0 R /K 4568 0 R /S /P >> endobj 5278 0 obj << /Pg 5060 0 R /P 42 0 R /K [4569 0 R 4570 0 R] /S /P >> endobj 5279 0 obj << /Pg 5060 0 R /P 42 0 R /K [4571 0 R 4572 0 R 4573 0 R 4574 0 R 4575 0 R 4576 0 R 4577 0 R 4578 0 R 4579 0 R 4580 0 R 4581 0 R 4582 0 R 4583 0 R 4584 0 R 4585 0 R 4586 0 R 4587 0 R 4588 0 R 4589 0 R] /S /P >> endobj 5280 0 obj << /Pg 5060 0 R /P 42 0 R /K 4590 0 R /S /P >> endobj 5281 0 obj << /Pg 5060 0 R /P 42 0 R /K [4591 0 R 4592 0 R] /S /P >> endobj 5282 0 obj << /Pg 5060 0 R /P 42 0 R /K [4593 0 R 4594 0 R 4595 0 R 4596 0 R 4597 0 R 4598 0 R 4599 0 R 4600 0 R 4601 0 R 4602 0 R 4603 0 R] /S /P >> endobj 5283 0 obj << /Pg 5060 0 R /P 42 0 R /K 4604 0 R /S /P >> endobj 5284 0 obj << /Pg 5060 0 R /P 42 0 R /K [4605 0 R 4606 0 R] /S /P >> endobj 5045 0 obj << /Pg 5044 0 R /P 42 0 R /K [4607 0 R 4608 0 R 4609 0 R] /S /P >> endobj 5046 0 obj << /Pg 5044 0 R /P 42 0 R /K [4610 0 R 4611 0 R 4612 0 R 4613 0 R 4614 0 R 4615 0 R 4616 0 R 4617 0 R 4618 0 R 4619 0 R 4620 0 R 4621 0 R 4622 0 R] /S /P >> endobj 5047 0 obj << /Pg 5044 0 R /P 42 0 R /K [4623 0 R 4624 0 R 4625 0 R 4626 0 R 4627 0 R 4628 0 R 4629 0 R 4630 0 R 4631 0 R 4632 0 R] /S /P >> endobj 5048 0 obj << /Pg 5044 0 R /P 42 0 R /K [4633 0 R 4634 0 R 4635 0 R 4636 0 R 4637 0 R 4638 0 R 4639 0 R 4640 0 R 4641 0 R 4642 0 R 4643 0 R 4644 0 R 4645 0 R 4646 0 R 4647 0 R 4648 0 R] /S /P >> endobj 5049 0 obj << /Pg 5044 0 R /P 42 0 R /K [4649 0 R 4650 0 R 4651 0 R 4652 0 R 4653 0 R 4654 0 R 4655 0 R 4656 0 R 4657 0 R 4658 0 R 4659 0 R 4660 0 R 4661 0 R] /S /P >> endobj 5050 0 obj << /Pg 5044 0 R /P 42 0 R /K [4662 0 R 4663 0 R 4664 0 R 4665 0 R 4666 0 R 4667 0 R 4668 0 R 4669 0 R 4670 0 R 4671 0 R 4672 0 R 4673 0 R 4674 0 R 4675 0 R 4676 0 R] /S /P >> endobj 5051 0 obj << /Pg 5044 0 R /P 42 0 R /K [4677 0 R 4678 0 R 4679 0 R 4680 0 R 4681 0 R 4682 0 R 4683 0 R 4684 0 R 4685 0 R 4686 0 R 4687 0 R 4688 0 R 4689 0 R 4690 0 R 4691 0 R 4692 0 R 4693 0 R] /S /P >> endobj 5052 0 obj << /Pg 5044 0 R /P 42 0 R /K 4694 0 R /S /P >> endobj 4605 0 obj << /Pg 5060 0 R /P 5284 0 R /K 40 /ActualText ( ) /S /Span >> endobj 4606 0 obj << /Pg 5060 0 R /P 5284 0 R /K 41 /ActualText ( ) /S /Span >> endobj 4604 0 obj << /Pg 5060 0 R /P 5283 0 R /K 39 /ActualText ( ) /S /Span >> endobj 4593 0 obj << /Pg 5060 0 R /P 5282 0 R /K 28 /ActualText (One) /S /Span >> endobj 4594 0 obj << /Pg 5060 0 R /P 5282 0 R /K 29 /ActualText (-) /S /Span >> endobj 4595 0 obj << /Pg 5060 0 R /P 5282 0 R /K 30 /ActualText (way analysis of variance \(ANOVA\) was used to analyze the statistical differences among ) /S /Span >> endobj 4596 0 obj << /Pg 5060 0 R /P 5282 0 R /K 31 /ActualText (the groups, with P values indicated in the related graphs. Differences between 2 groups were ) /S /Span >> endobj 4597 0 obj << /Pg 5060 0 R /P 5282 0 R /K 32 /ActualText (identified using the Student s t) /S /Span >> endobj 4598 0 obj << /Pg 5060 0 R /P 5282 0 R /K 33 /ActualText (-) /S /Span >> endobj 4599 0 obj << /Pg 5060 0 R /P 5282 0 R /K 34 /ActualText (test. All data are expressed as mean ) /S /Span >> endobj 4600 0 obj << /Pg 5060 0 R /P 5282 0 R /K 35 /ActualText (s.e.m.. The analyses and ) /S /Span >> endobj 4601 0 obj << /Pg 5060 0 R /P 5282 0 R /K 36 /ActualText (graphs were performed using GraphPad Prism 7.0 software \(GraphPad Software Inc., San ) /S /Span >> endobj 4602 0 obj << /Pg 5060 0 R /P 5282 0 R /K 37 /ActualText (Diego, CA, USA\). A value of P less than 0.05 was considered statistically significant.) /S /Span >> endobj 4603 0 obj << /Pg 5060 0 R /P 5282 0 R /K 38 /ActualText ( ) /S /Span >> endobj 4591 0 obj << /Pg 5060 0 R /P 5281 0 R /K 26 /ActualText (Statistical analysis) /S /Span >> endobj 4592 0 obj << /Pg 5060 0 R /P 5281 0 R /K 27 /ActualText ( ) /S /Span >> endobj 4590 0 obj << /Pg 5060 0 R /P 5280 0 R /K 25 /ActualText ( ) /S /Span >> endobj 4571 0 obj << /Pg 5060 0 R /P 5279 0 R /K 6 /ActualText (Serial longitudinal sections \(3) /S /Span >> endobj 4572 0 obj << /Pg 5060 0 R /P 5279 0 R /K 7 /ActualText (-) /S /Span >> endobj 4573 0 obj << /Pg 5060 0 R /P 5279 0 R /K 8 /ActualText (m thick\) stained with hematoxylin/eosin \(H&E\) were used for ) /S /Span >> endobj 4574 0 obj << /Pg 5060 0 R /P 5279 0 R /K 9 /ActualText (morphological analysis. Ten randomly selected \(100x\) fields per slide were photo) /S /Span >> endobj 4575 0 obj << /Pg 5060 0 R /P 5279 0 R /K 10 /ActualText (graphed ) /S /Span >> endobj 4576 0 obj << /Pg 5060 0 R /P 5279 0 R /K 11 /ActualText (using Olympus BX53 inverted microscope \(Olympus, Melville, NY\). The images were ) /S /Span >> endobj 4577 0 obj << /Pg 5060 0 R /P 5279 0 R /K 12 /ActualText (analyzed using the modified Image J software \(open source\), as described ) /S /Span >> endobj 4578 0 obj << /Pg 5060 0 R /P 5279 0 R /K 13 /ActualText (previously) /S /Span >> endobj 4579 0 obj << /Pg 5060 0 R /P 5279 0 R /K 14 /ActualText ([6]) /S /Span >> endobj 4580 0 obj << /Pg 5060 0 R /P 5279 0 R /K 15 /ActualText (.) /S /Span >> endobj 4581 0 obj << /Pg 5060 0 R /P 5279 0 R /K 16 /ActualText ( ) /S /Span >> endobj 4582 0 obj << /Pg 5060 0 R /P 5279 0 R /K 17 /ActualText (From each field, 10) /S /Span >> endobj 4583 0 obj << /Pg 5060 0 R /P 5279 0 R /K 18 /ActualText (-) /S /Span >> endobj 4584 0 obj << /Pg 5060 0 R /P 5279 0 R /K 19 /ActualText (12 areas of interest, free of airways and blood vessels, were picked for ) /S /Span >> endobj 4585 0 obj << /Pg 5060 0 R /P 5279 0 R /K 20 /ActualText (measurement of the number of intersections of virtual lines of kn) /S /Span >> endobj 4586 0 obj << /Pg 5060 0 R /P 5279 0 R /K 21 /ActualText (own length, with alveolar ) /S /Span >> endobj 4587 0 obj << /Pg 5060 0 R /P 5279 0 R /K 22 /ActualText (septa. An increase in the average distance between intercepts \(mean linear intercept\) indicates ) /S /Span >> endobj 4588 0 obj << /Pg 5060 0 R /P 5279 0 R /K 23 /ActualText (enlarged airspaces.) /S /Span >> endobj 4589 0 obj << /Pg 5060 0 R /P 5279 0 R /K 24 /ActualText ( ) /S /Span >> endobj 4569 0 obj << /Pg 5060 0 R /P 5278 0 R /K 4 /ActualText (Morphological analysis of lung sections ) /S /Span >> endobj 4570 0 obj << /Pg 5060 0 R /P 5278 0 R /K 5 /ActualText ( ) /S /Span >> endobj 4568 0 obj << /Pg 5060 0 R /P 5277 0 R /K 3 /ActualText ( ) /S /Span >> endobj 4565 0 obj << /Pg 5060 0 R /P 5276 0 R /K 0 /ActualText (minimum number of three ti) /S /Span >> endobj 4566 0 obj << /Pg 5060 0 R /P 5276 0 R /K 1 /ActualText (mes to ensure reasonable repeatability. ) /S /Span >> endobj 4567 0 obj << /Pg 5060 0 R /P 5276 0 R /K 2 /ActualText ( ) /S /Span >> endobj 4546 0 obj << /Pg 5059 0 R /P 5275 0 R /K 120 /ActualText (Mice were sedated with 0.1 g/kg ) /S /Span >> endobj 4547 0 obj << /Pg 5059 0 R /P 5275 0 R /K 121 /ActualText (intraperitoneal and 1% pentobarbital sodium, and the dose ) /S /Span >> endobj 4548 0 obj << /Pg 5059 0 R /P 5275 0 R /K 122 /ActualText (could be adjusted as necessary. The mice were intubated with a 2) /S /Span >> endobj 4549 0 obj << /Pg 5059 0 R /P 5275 0 R /K 123 /ActualText (-) /S /Span >> endobj 4550 0 obj << /Pg 5059 0 R /P 5275 0 R /K 124 /ActualText (in) /S /Span >> endobj 4551 0 obj << /Pg 5059 0 R /P 5275 0 R /K 125 /ActualText (-) /S /Span >> endobj 4552 0 obj << /Pg 5059 0 R /P 5275 0 R /K 126 /ActualText (long, 14) /S /Span >> endobj 4553 0 obj << /Pg 5059 0 R /P 5275 0 R /K 127 /ActualText (-) /S /Span >> endobj 4554 0 obj << /Pg 5059 0 R /P 5275 0 R /K 128 /ActualText (gauge ) /S /Span >> endobj 4555 0 obj << /Pg 5059 0 R /P 5275 0 R /K 129 /ActualText (angiocatheter which then connected to a rodent) /S /Span >> endobj 4556 0 obj << /Pg 5059 0 R /P 5275 0 R /K 130 /ActualText (-) /S /Span >> endobj 4557 0 obj << /Pg 5059 0 R /P 5275 0 R /K 131 /ActualText (specific forced maneuver system \(Pulmonary ) /S /Span >> endobj 4558 0 obj << /Pg 5059 0 R /P 5275 0 R /K 132 /ActualText (Function Testing, Buxco R) /S /Span >> endobj 4559 0 obj << /Pg 5059 0 R /P 5275 0 R /K 133 /ActualText (esearch Systems, USA\). A series of preprogrammed forced ) /S /Span >> endobj 4560 0 obj << /Pg 5059 0 R /P 5275 0 R /K 134 /ActualText (ventilation maneuvers were used to measure FVC \(forced vital capacity\) and FEV) /S /Span >> endobj 4561 0 obj << /Pg 5059 0 R /P 5275 0 R /K 135 /ActualText (20) /S /Span >> endobj 4562 0 obj << /Pg 5059 0 R /P 5275 0 R /K 136 /ActualText ( ) /S /Span >> endobj 4563 0 obj << /Pg 5059 0 R /P 5275 0 R /K 137 /ActualText (\(forced ) /S /Span >> endobj 4564 0 obj << /Pg 5059 0 R /P 5275 0 R /K 138 /ActualText (expiratory volume in 20 seconds\) within 10 minutes. Each measurement was performed a ) /S /Span >> endobj 4544 0 obj << /Pg 5059 0 R /P 5274 0 R /K 118 /ActualText (Measurement of lung function) /S /Span >> endobj 4545 0 obj << /Pg 5059 0 R /P 5274 0 R /K 119 /ActualText ( ) /S /Span >> endobj 4543 0 obj << /Pg 5059 0 R /P 5273 0 R /K 117 /ActualText ( ) /S /Span >> endobj 4476 0 obj << /Pg 5059 0 R /P 5272 0 R /K 50 /ActualText (Mouse lung white) /S /Span >> endobj 4477 0 obj << /Pg 5059 0 R /P 5272 0 R /K 51 /ActualText ( ) /S /Span >> endobj 4478 0 obj << /Pg 5059 0 R /P 5272 0 R /K 52 /ActualText (blood) /S /Span >> endobj 4479 0 obj << /Pg 5059 0 R /P 5272 0 R /K 53 /ActualText ( ) /S /Span >> endobj 4480 0 obj << /Pg 5059 0 R /P 5272 0 R /K 54 /ActualText (cells) /S /Span >> endobj 4481 0 obj << /Pg 5059 0 R /P 5272 0 R /K 55 /ActualText ( ) /S /Span >> endobj 4482 0 obj << /Pg 5059 0 R /P 5272 0 R /K 56 /ActualText (or ) /S /Span >> endobj 4483 0 obj << /Pg 5059 0 R /P 5272 0 R /K 57 /ActualText (human peripheral blood ) /S /Span >> endobj 4484 0 obj << /Pg 5059 0 R /P 5272 0 R /K 58 /ActualText (mononuclear) /S /Span >> endobj 4485 0 obj << /Pg 5059 0 R /P 5272 0 R /K 59 /ActualText ( ) /S /Span >> endobj 4486 0 obj << /Pg 5059 0 R /P 5272 0 R /K 60 /ActualText (cells were stained with ) /S /Span >> endobj 4487 0 obj << /Pg 5059 0 R /P 5272 0 R /K 61 /ActualText (indicated antibodies and were analysed by BD Fortessa LSR. ) /S /Span >> endobj 4488 0 obj << /Pg 5059 0 R /P 5272 0 R /K 62 /ActualText (The samples were analysed ) /S /Span >> endobj 4489 0 obj << /Pg 5059 0 R /P 5272 0 R /K 63 /ActualText (using the Flowjo software \(Treestar LLC. Ashland, Oregon, USA\). Anti) /S /Span >> endobj 4490 0 obj << /Pg 5059 0 R /P 5272 0 R /K 64 /ActualText (-) /S /Span >> endobj 4491 0 obj << /Pg 5059 0 R /P 5272 0 R /K 65 /ActualText (Mouse CD45 ) /S /Span >> endobj 4492 0 obj << /Pg 5059 0 R /P 5272 0 R /K 66 /ActualText (perCP) /S /Span >> endobj 4493 0 obj << /Pg 5059 0 R /P 5272 0 R /K 67 /ActualText (-) /S /Span >> endobj 4494 0 obj << /Pg 5059 0 R /P 5272 0 R /K 68 /ActualText (Cyanine 5.5, Anti) /S /Span >> endobj 4495 0 obj << /Pg 5059 0 R /P 5272 0 R /K 69 /ActualText (-) /S /Span >> endobj 4496 0 obj << /Pg 5059 0 R /P 5272 0 R /K 70 /ActualText (Mouse) /S /Span >> endobj 4497 0 obj << /Pg 5059 0 R /P 5272 0 R /K 71 /ActualText ( ) /S /Span >> endobj 4498 0 obj << /Pg 5059 0 R /P 5272 0 R /K 72 /ActualText (CD3e) /S /Span >> endobj 4499 0 obj << /Pg 5059 0 R /P 5272 0 R /K 73 /ActualText ( ) /S /Span >> endobj 4500 0 obj << /Pg 5059 0 R /P 5272 0 R /K 74 /ActualText (APC) /S /Span >> endobj 4501 0 obj << /Pg 5059 0 R /P 5272 0 R /K 75 /ActualText (-) /S /Span >> endobj 4502 0 obj << /Pg 5059 0 R /P 5272 0 R /K 76 /ActualText (Cyanine7, Anti) /S /Span >> endobj 4503 0 obj << /Pg 5059 0 R /P 5272 0 R /K 77 /ActualText (-) /S /Span >> endobj 4504 0 obj << /Pg 5059 0 R /P 5272 0 R /K 78 /ActualText (Mouse CD8a PE) /S /Span >> endobj 4505 0 obj << /Pg 5059 0 R /P 5272 0 R /K 79 /ActualText (-) /S /Span >> endobj 4506 0 obj << /Pg 5059 0 R /P 5272 0 R /K 80 /ActualText (Cyanine 7, ) /S /Span >> endobj 4507 0 obj << /Pg 5059 0 R /P 5272 0 R /K 81 /ActualText (Anti) /S /Span >> endobj 4508 0 obj << /Pg 5059 0 R /P 5272 0 R /K 82 /ActualText (-) /S /Span >> endobj 4509 0 obj << /Pg 5059 0 R /P 5272 0 R /K 83 /ActualText (Mouse CD4 PE) /S /Span >> endobj 4510 0 obj << /Pg 5059 0 R /P 5272 0 R /K 84 /ActualText (-) /S /Span >> endobj 4511 0 obj << /Pg 5059 0 R /P 5272 0 R /K 85 /ActualText (Cyanine7, Anti) /S /Span >> endobj 4512 0 obj << /Pg 5059 0 R /P 5272 0 R /K 86 /ActualText (-) /S /Span >> endobj 4513 0 obj << /Pg 5059 0 R /P 5272 0 R /K 87 /ActualText (Mouse) /S /Span >> endobj 4514 0 obj << /Pg 5059 0 R /P 5272 0 R /K 88 /ActualText ( ) /S /Span >> endobj 4515 0 obj << /Pg 5059 0 R /P 5272 0 R /K 89 /ActualText (IFN) /S /Span >> endobj 4516 0 obj << /Pg 5059 0 R /P 5272 0 R /K 90 /ActualText (G ) /S /Span >> endobj 4517 0 obj << /Pg 5059 0 R /P 5272 0 R /K 91 /ActualText (FITC, Anti) /S /Span >> endobj 4518 0 obj << /Pg 5059 0 R /P 5272 0 R /K 92 /ActualText (-) /S /Span >> endobj 4519 0 obj << /Pg 5059 0 R /P 5272 0 R /K 93 /ActualText (Mouse IL17) /S /Span >> endobj 4520 0 obj << /Pg 5059 0 R /P 5272 0 R /K 94 /ActualText (A ) /S /Span >> endobj 4521 0 obj << /Pg 5059 0 R /P 5272 0 R /K 95 /ActualText (APC,) /S /Span >> endobj 4522 0 obj << /Pg 5059 0 R /P 5272 0 R /K 96 /ActualText ( ) /S /Span >> endobj 4523 0 obj << /Pg 5059 0 R /P 5272 0 R /K 97 /ActualText (Anti) /S /Span >> endobj 4524 0 obj << /Pg 5059 0 R /P 5272 0 R /K 98 /ActualText (-) /S /Span >> endobj 4525 0 obj << /Pg 5059 0 R /P 5272 0 R /K 99 /ActualText (human CD45 APC, Anti) /S /Span >> endobj 4526 0 obj << /Pg 5059 0 R /P 5272 0 R /K 100 /ActualText (-) /S /Span >> endobj 4527 0 obj << /Pg 5059 0 R /P 5272 0 R /K 101 /ActualText (human CD8a eFluor450, Anti) /S /Span >> endobj 4528 0 obj << /Pg 5059 0 R /P 5272 0 R /K 102 /ActualText (-) /S /Span >> endobj 4529 0 obj << /Pg 5059 0 R /P 5272 0 R /K 103 /ActualText (human CD3 PE) /S /Span >> endobj 4530 0 obj << /Pg 5059 0 R /P 5272 0 R /K 104 /ActualText (-) /S /Span >> endobj 4531 0 obj << /Pg 5059 0 R /P 5272 0 R /K 105 /ActualText (Cyanine 7, ) /S /Span >> endobj 4532 0 obj << /Pg 5059 0 R /P 5272 0 R /K 106 /ActualText (Anti) /S /Span >> endobj 4533 0 obj << /Pg 5059 0 R /P 5272 0 R /K 107 /ActualText (-) /S /Span >> endobj 4534 0 obj << /Pg 5059 0 R /P 5272 0 R /K 108 /ActualText (human IFN) /S /Span >> endobj 4535 0 obj << /Pg 5059 0 R /P 5272 0 R /K 109 /ActualText (G ) /S /Span >> endobj 4536 0 obj << /Pg 5059 0 R /P 5272 0 R /K 110 /ActualText (Alexa Fluor488, and Anti) /S /Span >> endobj 4537 0 obj << /Pg 5059 0 R /P 5272 0 R /K 111 /ActualText (-) /S /Span >> endobj 4538 0 obj << /Pg 5059 0 R /P 5272 0 R /K 112 /ActualText (human IL) /S /Span >> endobj 4539 0 obj << /Pg 5059 0 R /P 5272 0 R /K 113 /ActualText (-) /S /Span >> endobj 4540 0 obj << /Pg 5059 0 R /P 5272 0 R /K 114 /ActualText (17A PE were all purchased from ) /S /Span >> endobj 4541 0 obj << /Pg 5059 0 R /P 5272 0 R /K 115 /ActualText (eBioscience, San Diego, CA, USA.) /S /Span >> endobj 4542 0 obj << /Pg 5059 0 R /P 5272 0 R /K 116 /ActualText ( ) /S /Span >> endobj 4474 0 obj << /Pg 5059 0 R /P 5271 0 R /K 48 /ActualText (Flow cytometry ) /S /Span >> endobj 4475 0 obj << /Pg 5059 0 R /P 5271 0 R /K 49 /ActualText ( ) /S /Span >> endobj 4473 0 obj << /Pg 5059 0 R /P 5270 0 R /K 47 /ActualText ( ) /S /Span >> endobj 4462 0 obj << /Pg 5059 0 R /P 5269 0 R /K 36 /ActualText (PBMC pellets were suspended and incubated in human T cell culture medium containing 5% ) /S /Span >> endobj 4463 0 obj << /Pg 5059 0 R /P 5269 0 R /K 37 /ActualText (autoplasma for 7 days in the presence of IL2 \(R&D systems\). Cells were then harvested and ) /S /Span >> endobj 4464 0 obj << /Pg 5059 0 R /P 5269 0 R /K 38 /ActualText (stained for GVAPGVGVAPGV/HLA) /S /Span >> endobj 4465 0 obj << /Pg 5059 0 R /P 5269 0 R /K 39 /ActualText (-) /S /Span >> endobj 4466 0 obj << /Pg 5059 0 R /P 5269 0 R /K 40 /ActualText (A*02:01 tetramer, anti) /S /Span >> endobj 4467 0 obj << /Pg 5059 0 R /P 5269 0 R /K 41 /ActualText (-) /S /Span >> endobj 4468 0 obj << /Pg 5059 0 R /P 5269 0 R /K 42 /ActualText (CD3, and anti) /S /Span >> endobj 4469 0 obj << /Pg 5059 0 R /P 5269 0 R /K 43 /ActualText (-) /S /Span >> endobj 4470 0 obj << /Pg 5059 0 R /P 5269 0 R /K 44 /ActualText (CD8 antibodies ) /S /Span >> endobj 4471 0 obj << /Pg 5059 0 R /P 5269 0 R /K 45 /ActualText (\(eBioscience\) for analysis of tetramer positive cells by flow cytometry.) /S /Span >> endobj 4472 0 obj << /Pg 5059 0 R /P 5269 0 R /K 46 /ActualText ( ) /S /Span >> endobj 4450 0 obj << /Pg 5059 0 R /P 5268 0 R /K 24 /ActualText (PE) /S /Span >> endobj 4451 0 obj << /Pg 5059 0 R /P 5268 0 R /K 25 /ActualText (-) /S /Span >> endobj 4452 0 obj << /Pg 5059 0 R /P 5268 0 R /K 26 /ActualText (conjugated GVAPGVGVAPGV/HLA) /S /Span >> endobj 4453 0 obj << /Pg 5059 0 R /P 5268 0 R /K 27 /ActualText (-) /S /Span >> endobj 4454 0 obj << /Pg 5059 0 R /P 5268 0 R /K 28 /ActualText (A*02:01 tetramer was s) /S /Span >> endobj 4455 0 obj << /Pg 5059 0 R /P 5268 0 R /K 29 /ActualText (ynthesized according to ) /S /Span >> endobj 4456 0 obj << /Pg 5059 0 R /P 5268 0 R /K 30 /ActualText (the protocol published ) /S /Span >> endobj 4457 0 obj << /Pg 5059 0 R /P 5268 0 R /K 31 /ActualText (previously) /S /Span >> endobj 4458 0 obj << /Pg 5059 0 R /P 5268 0 R /K 32 /ActualText ( ) /S /Span >> endobj 4459 0 obj << /Pg 5059 0 R /P 5268 0 R /K 33 /ActualText ([5]) /S /Span >> endobj 4460 0 obj << /Pg 5059 0 R /P 5268 0 R /K 34 /ActualText (. ) /S /Span >> endobj 4461 0 obj << /Pg 5059 0 R /P 5268 0 R /K 35 /ActualText ( ) /S /Span >> endobj 4426 0 obj << /Pg 5059 0 R /P 5267 0 R /K 0 /ActualText (possible sub) /S /Span >> endobj 4427 0 obj << /Pg 5059 0 R /P 5267 0 R /K 1 /ActualText (-) /S /Span >> endobj 4428 0 obj << /Pg 5059 0 R /P 5267 0 R /K 2 /ActualText (peptides \(with 8) /S /Span >> endobj 4429 0 obj << /Pg 5059 0 R /P 5267 0 R /K 3 /ActualText (-) /S /Span >> endobj 4430 0 obj << /Pg 5059 0 R /P 5267 0 R /K 4 /ActualText (14 residues\) hosted within the full) /S /Span >> endobj 4431 0 obj << /Pg 5059 0 R /P 5267 0 R /K 5 /ActualText (-) /S /Span >> endobj 4432 0 obj << /Pg 5059 0 R /P 5267 0 R /K 6 /ActualText (length were predicted to bind ) /S /Span >> endobj 4433 0 obj << /Pg 5059 0 R /P 5267 0 R /K 7 /ActualText (with all human HLA) /S /Span >> endobj 4434 0 obj << /Pg 5059 0 R /P 5267 0 R /K 8 /ActualText (-) /S /Span >> endobj 4435 0 obj << /Pg 5059 0 R /P 5267 0 R /K 9 /ActualText (A, ) /S /Span >> endobj 4436 0 obj << /Pg 5059 0 R /P 5267 0 R /K 10 /ActualText (-) /S /Span >> endobj 4437 0 obj << /Pg 5059 0 R /P 5267 0 R /K 11 /ActualText (B, ) /S /Span >> endobj 4438 0 obj << /Pg 5059 0 R /P 5267 0 R /K 12 /ActualText (-) /S /Span >> endobj 4439 0 obj << /Pg 5059 0 R /P 5267 0 R /K 13 /ActualText (C alleles in IEDB recommended 2.19 database. The predicted ) /S /Span >> endobj 4440 0 obj << /Pg 5059 0 R /P 5267 0 R /K 14 /ActualText (output is given in units of IC50 \(nmol/l\). Peptides with IC50 values <50 n) /S /Span >> endobj 4441 0 obj << /Pg 5059 0 R /P 5267 0 R /K 15 /ActualText (mol/l are considered ) /S /Span >> endobj 4442 0 obj << /Pg 5059 0 R /P 5267 0 R /K 16 /ActualText (with high affinity. GVAPGVGVAPGV and HLA) /S /Span >> endobj 4443 0 obj << /Pg 5059 0 R /P 5267 0 R /K 17 /ActualText (-) /S /Span >> endobj 4444 0 obj << /Pg 5059 0 R /P 5267 0 R /K 18 /ActualText (A*02:01 were paired as they were among ) /S /Span >> endobj 4445 0 obj << /Pg 5059 0 R /P 5267 0 R /K 19 /ActualText (those with high or intermediate affinity in prediction, and HLA) /S /Span >> endobj 4446 0 obj << /Pg 5059 0 R /P 5267 0 R /K 20 /ActualText (-) /S /Span >> endobj 4447 0 obj << /Pg 5059 0 R /P 5267 0 R /K 21 /ActualText (A*02:01 is a common allele ) /S /Span >> endobj 4448 0 obj << /Pg 5059 0 R /P 5267 0 R /K 22 /ActualText (in human. ) /S /Span >> endobj 4449 0 obj << /Pg 5059 0 R /P 5267 0 R /K 23 /ActualText ( ) /S /Span >> endobj 4421 0 obj << /Pg 5058 0 R /P 5266 0 R /K 72 /ActualText (Epitope prediction was performed on 2018/4/20 with the IEDB Analysis Resource web ) /S /Span >> endobj 4422 0 obj << /Pg 5058 0 R /P 5266 0 R /K 73 /ActualText (interface) /S /Span >> endobj 4423 0 obj << /Pg 5058 0 R /P 5266 0 R /K 74 /ActualText ( ) /S /Span >> endobj 4424 0 obj << /Pg 5058 0 R /P 5266 0 R /K 75 /ActualText ([3, 4]) /S /Span >> endobj 4425 0 obj << /Pg 5058 0 R /P 5266 0 R /K 76 /ActualText (. Briefly, the repeating peptide VGVAPG was input as specific sequence, all ) /S /Span >> endobj 4417 0 obj << /Pg 5058 0 R /P 5265 0 R /K 68 /ActualText (GVAPGVGVAPGV/HLA) /S /Span >> endobj 4418 0 obj << /Pg 5058 0 R /P 5265 0 R /K 69 /ActualText (-) /S /Span >> endobj 4419 0 obj << /Pg 5058 0 R /P 5265 0 R /K 70 /ActualText (A*02:01 tetramer prediction, synthesis and staining ) /S /Span >> endobj 4420 0 obj << /Pg 5058 0 R /P 5265 0 R /K 71 /ActualText ( ) /S /Span >> endobj 4416 0 obj << /Pg 5058 0 R /P 5264 0 R /K 67 /ActualText ( ) /S /Span >> endobj 4398 0 obj << /Pg 5058 0 R /P 5263 0 R /K 49 /ActualText (Heparin) /S /Span >> endobj 4399 0 obj << /Pg 5058 0 R /P 5263 0 R /K 50 /ActualText (-) /S /Span >> endobj 4400 0 obj << /Pg 5058 0 R /P 5263 0 R /K 51 /ActualText (anticoagulated ) /S /Span >> endobj 4401 0 obj << /Pg 5058 0 R /P 5263 0 R /K 52 /ActualText (peripheral blood from COPD patients or healthy controls were ) /S /Span >> endobj 4402 0 obj << /Pg 5058 0 R /P 5263 0 R /K 53 /ActualText (collected and centrifuged at 400 \t \tg for 5 min and the top layer containing plasma was ) /S /Span >> endobj 4403 0 obj << /Pg 5058 0 R /P 5263 0 R /K 54 /ActualText (removed. The remaining blood was diluted with an equal volume of normal saline, 5 ml of ) /S /Span >> endobj 4404 0 obj << /Pg 5058 0 R /P 5263 0 R /K 55 /ActualText (diluted blood was la) /S /Span >> endobj 4405 0 obj << /Pg 5058 0 R /P 5263 0 R /K 56 /ActualText (yered over 5 ml of the Ficoll) /S /Span >> endobj 4406 0 obj << /Pg 5058 0 R /P 5263 0 R /K 57 /ActualText (-) /S /Span >> endobj 4407 0 obj << /Pg 5058 0 R /P 5263 0 R /K 58 /ActualText (Paque PLUS \(GE Healthcare\). Gradients ) /S /Span >> endobj 4408 0 obj << /Pg 5058 0 R /P 5263 0 R /K 59 /ActualText (were centrifuged at 400 \t \tg for 30 min at room temperature in a swinging) /S /Span >> endobj 4409 0 obj << /Pg 5058 0 R /P 5263 0 R /K 60 /ActualText (-) /S /Span >> endobj 4410 0 obj << /Pg 5058 0 R /P 5263 0 R /K 61 /ActualText (bucket rotor ) /S /Span >> endobj 4411 0 obj << /Pg 5058 0 R /P 5263 0 R /K 62 /ActualText (without the brake applied. The PBMC interface was carefully removed by pipetting and ) /S /Span >> endobj 4412 0 obj << /Pg 5058 0 R /P 5263 0 R /K 63 /ActualText (washed with norma) /S /Span >> endobj 4413 0 obj << /Pg 5058 0 R /P 5263 0 R /K 64 /ActualText (l saline by centrifugation at 400 \t \tg for 5 min. PBMC were prepared for ) /S /Span >> endobj 4414 0 obj << /Pg 5058 0 R /P 5263 0 R /K 65 /ActualText (further use.) /S /Span >> endobj 4415 0 obj << /Pg 5058 0 R /P 5263 0 R /K 66 /ActualText ( ) /S /Span >> endobj 4396 0 obj << /Pg 5058 0 R /P 5262 0 R /K 47 /ActualText (PBMC isolation) /S /Span >> endobj 4397 0 obj << /Pg 5058 0 R /P 5262 0 R /K 48 /ActualText ( ) /S /Span >> endobj 4395 0 obj << /Pg 5058 0 R /P 5261 0 R /K 46 /ActualText ( ) /S /Span >> endobj 4379 0 obj << /Pg 5058 0 R /P 5260 0 R /K 30 /ActualText (EDTA) /S /Span >> endobj 4380 0 obj << /Pg 5058 0 R /P 5260 0 R /K 31 /ActualText (-) /S /Span >> endobj 4381 0 obj << /Pg 5058 0 R /P 5260 0 R /K 32 /ActualText (anticoagulated peripheral blood from COPD patients or healthy controls were ) /S /Span >> endobj 4382 0 obj << /Pg 5058 0 R /P 5260 0 R /K 33 /ActualText (collected, and genomic DNA was extracted using a universal genomic DNA kit \(CWbiotech) /S /Span >> endobj 4383 0 obj << /Pg 5058 0 R /P 5260 0 R /K 34 /ActualText (, ) /S /Span >> endobj 4384 0 obj << /Pg 5058 0 R /P 5260 0 R /K 35 /ActualText (China) /S /Span >> endobj 4385 0 obj << /Pg 5058 0 R /P 5260 0 R /K 36 /ActualText (\) following the manufacturer s procedure. High resolut) /S /Span >> endobj 4386 0 obj << /Pg 5058 0 R /P 5260 0 R /K 37 /ActualText (ion genotyping of HLA) /S /Span >> endobj 4387 0 obj << /Pg 5058 0 R /P 5260 0 R /K 38 /ActualText (-) /S /Span >> endobj 4388 0 obj << /Pg 5058 0 R /P 5260 0 R /K 39 /ActualText (A alleles ) /S /Span >> endobj 4389 0 obj << /Pg 5058 0 R /P 5260 0 R /K 40 /ActualText (was performed by Immunogenetics Laboratory, Blood center for Zhejiang Province using ) /S /Span >> endobj 4390 0 obj << /Pg 5058 0 R /P 5260 0 R /K 41 /ActualText (commercial polymerase chain reaction \(PCR\) plus sequence) /S /Span >> endobj 4391 0 obj << /Pg 5058 0 R /P 5260 0 R /K 42 /ActualText (-) /S /Span >> endobj 4392 0 obj << /Pg 5058 0 R /P 5260 0 R /K 43 /ActualText (based typing \(SBT\) kit \(One ) /S /Span >> endobj 4393 0 obj << /Pg 5058 0 R /P 5260 0 R /K 44 /ActualText (Lambda, Inc.\).) /S /Span >> endobj 4394 0 obj << /Pg 5058 0 R /P 5260 0 R /K 45 /ActualText ( ) /S /Span >> endobj 4375 0 obj << /Pg 5058 0 R /P 5259 0 R /K 26 /ActualText (Genotyping of HLA) /S /Span >> endobj 4376 0 obj << /Pg 5058 0 R /P 5259 0 R /K 27 /ActualText (-) /S /Span >> endobj 4377 0 obj << /Pg 5058 0 R /P 5259 0 R /K 28 /ActualText (A alleles ) /S /Span >> endobj 4378 0 obj << /Pg 5058 0 R /P 5259 0 R /K 29 /ActualText ( ) /S /Span >> endobj 4374 0 obj << /Pg 5058 0 R /P 5258 0 R /K 25 /ActualText ( ) /S /Span >> endobj 4363 0 obj << /Pg 5058 0 R /P 5257 0 R /K 14 /ActualText (Sputum was induced as previously ) /S /Span >> endobj 4364 0 obj << /Pg 5058 0 R /P 5257 0 R /K 15 /ActualText (described) /S /Span >> endobj 4365 0 obj << /Pg 5058 0 R /P 5257 0 R /K 16 /ActualText ( ) /S /Span >> endobj 4366 0 obj << /Pg 5058 0 R /P 5257 0 R /K 17 /ActualText ([2]) /S /Span >> endobj 4367 0 obj << /Pg 5058 0 R /P 5257 0 R /K 18 /ActualText (. A sample was considered adequate when the ) /S /Span >> endobj 4368 0 obj << /Pg 5058 0 R /P 5257 0 R /K 19 /ActualText (patient was able to expectorate at least 2 ml of sputum and the slides contained <10% ) /S /Span >> endobj 4369 0 obj << /Pg 5058 0 R /P 5257 0 R /K 20 /ActualText (squamous cells on differential cell counting. 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Images were scanned with an Olympus BX53 inverted ) /S /Span >> endobj 4348 0 obj << /Pg 5286 0 R /P 5253 0 R /K 119 /ActualText (microscope \(Olympus, Melville, NY\), and image quantitative analysis was performed as ) /S /Span >> endobj 4340 0 obj << /Pg 5286 0 R /P 5252 0 R /K 111 /ActualText (Immunohistochemistry and Immunofluorescence) /S /Span >> endobj 4341 0 obj << /Pg 5286 0 R /P 5252 0 R /K 112 /ActualText ( ) /S /Span >> endobj 4339 0 obj << /Pg 5286 0 R /P 5251 0 R /K 110 /ActualText ( ) /S /Span >> endobj 4308 0 obj << /Pg 5286 0 R /P 5250 0 R /K 79 /ActualText (The left lobes of lungs were fixed in ) /S /Span >> endobj 4309 0 obj << /Pg 5286 0 R /P 5250 0 R /K 80 /ActualText (4% paraformaldehyde) /S /Span >> endobj 4310 0 obj << /Pg 5286 0 R /P 5250 0 R /K 81 /ActualText ( ) /S /Span >> endobj 4311 0 obj << /Pg 5286 0 R /P 5250 0 R /K 82 /ActualText (at 4C for 24 h) /S /Span >> endobj 4312 0 obj << /Pg 5286 0 R /P 5250 0 R /K 83 /ActualText (. After paraffin ) /S /Span >> endobj 4313 0 obj << /Pg 5286 0 R /P 5250 0 R /K 84 /ActualText (embedding, the tissue sections were prepared \(3 m\). Lung sections were stained with ) /S /Span >> endobj 4314 0 obj << /Pg 5286 0 R /P 5250 0 R /K 85 /ActualText (hematoxylin/eosin \(H&E\), PAS or Masson trichrome. The H&E staining sections were ) /S /Span >> endobj 4315 0 obj << /Pg 5286 0 R /P 5250 0 R /K 86 /ActualText (semi) /S /Span >> endobj 4316 0 obj << /Pg 5286 0 R /P 5250 0 R /K 87 /ActualText (-) /S /Span >> endobj 4317 0 obj << /Pg 5286 0 R /P 5250 0 R /K 88 /ActualText (quantitated \(score: 0) /S /Span >> endobj 4318 0 obj << /Pg 5286 0 R /P 5250 0 R /K 89 /ActualText ( ) /S /Span >> endobj 4319 0 obj << /Pg 5286 0 R /P 5250 0 R /K 90 /ActualText (4\) with Olympus microscope \(10 20 magnification\) for the ) /S /Span >> endobj 4320 0 obj << /Pg 5286 0 R /P 5250 0 R /K 91 /ActualText (inflammatory situation. PAS stained goblet cells in airway epithelium were scored as ) /S /Span >> endobj 4321 0 obj << /Pg 5286 0 R /P 5250 0 R /K 92 /ActualText (described ) /S /Span >> endobj 4322 0 obj << /Pg 5286 0 R /P 5250 0 R /K 93 /ActualText (previously) /S /Span >> endobj 4323 0 obj << /Pg 5286 0 R /P 5250 0 R /K 94 /ActualText ( ) /S /Span >> endobj 4324 0 obj << /Pg 5286 0 R /P 5250 0 R /K 95 /ActualText (\() /S /Span >> endobj 4325 0 obj << /Pg 5286 0 R /P 5250 0 R /K 96 /ActualText (3\)) /S /Span >> endobj 4326 0 obj << /Pg 5286 0 R /P 5250 0 R /K 97 /ActualText (. Collagen area on the basal membrane of airway was analyzed ) /S /Span >> endobj 4327 0 obj << /Pg 5286 0 R /P 5250 0 R /K 98 /ActualText (by ) /S /Span >> endobj 4328 0 obj << /Pg 5286 0 R /P 5250 0 R /K 99 /ActualText (Leica) /S /Span >> endobj 4329 0 obj << /Pg 5286 0 R /P 5250 0 R /K 100 /ActualText (-) /S /Span >> endobj 4330 0 obj << /Pg 5286 0 R /P 5250 0 R /K 101 /ActualText (Qwin image) /S /Span >> endobj 4331 0 obj << /Pg 5286 0 R /P 5250 0 R /K 102 /ActualText (-) /S /Span >> endobj 4332 0 obj << /Pg 5286 0 R /P 5250 0 R /K 103 /ActualText (processing system \(Leica Imaging Systems, Bensheim, Germany\). The ) /S /Span >> endobj 4333 0 obj << /Pg 5286 0 R /P 5250 0 R /K 104 /ActualText (result was expressed as collagen staining area of per micrometer length of basement ) /S /Span >> endobj 4334 0 obj << /Pg 5286 0 R /P 5250 0 R /K 105 /ActualText (membrane of bronchioles. At least 10 bronchioles with 150 to 250 m of internal diamet) /S /Span >> endobj 4335 0 obj << /Pg 5286 0 R /P 5250 0 R /K 106 /ActualText (er ) /S /Span >> endobj 4336 0 obj << /Pg 5286 0 R /P 5250 0 R /K 107 /ActualText (were counted on each slide. All slides were examined in a random blinded fashion by 2 ) /S /Span >> endobj 4337 0 obj << /Pg 5286 0 R /P 5250 0 R /K 108 /ActualText (independent investigators.) /S /Span >> endobj 4338 0 obj << /Pg 5286 0 R /P 5250 0 R /K 109 /ActualText ( ) /S /Span >> endobj 4306 0 obj << /Pg 5286 0 R /P 5249 0 R /K 77 /ActualText (Lung histology and determination of collagen deposition) /S /Span >> endobj 4307 0 obj << /Pg 5286 0 R /P 5249 0 R /K 78 /ActualText ( ) /S /Span >> endobj 4305 0 obj << /Pg 5286 0 R /P 5248 0 R /K 76 /ActualText ( ) /S /Span >> endobj 4271 0 obj << /Pg 5286 0 R /P 5247 0 R /K 42 /ActualText (The concentration of ) /S /Span >> endobj 4272 0 obj << /Pg 5286 0 R /P 5247 0 R /K 43 /ActualText (CXCL1 ) /S /Span >> endobj 4273 0 obj << /Pg 5286 0 R /P 5247 0 R /K 44 /ActualText (\(MKC008B, R&D\), ) /S /Span >> endobj 4274 0 obj << /Pg 5286 0 R /P 5247 0 R /K 45 /ActualText (CXCL2 ) /S /Span >> endobj 4275 0 obj << /Pg 5286 0 R /P 5247 0 R /K 46 /ActualText (\(MM200, R&D\), ) /S /Span >> endobj 4276 0 obj << /Pg 5286 0 R /P 5247 0 R /K 47 /ActualText (IL6) /S /Span >> endobj 4277 0 obj << /Pg 5286 0 R /P 5247 0 R /K 48 /ActualText ( ) /S /Span >> endobj 4278 0 obj << /Pg 5286 0 R /P 5247 0 R /K 49 /ActualText (\(M60000B, ) /S /Span >> endobj 4279 0 obj << /Pg 5286 0 R /P 5247 0 R /K 50 /ActualText (R&D\), ) /S /Span >> endobj 4280 0 obj << /Pg 5286 0 R /P 5247 0 R /K 51 /ActualText (IFNG) /S /Span >> endobj 4281 0 obj << /Pg 5286 0 R /P 5247 0 R /K 52 /ActualText ( ) /S /Span >> endobj 4282 0 obj << /Pg 5286 0 R /P 5247 0 R /K 53 /ActualText (\(MZF00, R&D\), ) /S /Span >> endobj 4283 0 obj << /Pg 5286 0 R /P 5247 0 R /K 54 /ActualText (and ) /S /Span >> endobj 4284 0 obj << /Pg 5286 0 R /P 5247 0 R /K 55 /ActualText (IL17) /S /Span >> endobj 4285 0 obj << /Pg 5286 0 R /P 5247 0 R /K 56 /ActualText (A) /S /Span >> endobj 4286 0 obj << /Pg 5286 0 R /P 5247 0 R /K 57 /ActualText ( ) /S /Span >> endobj 4287 0 obj << /Pg 5286 0 R /P 5247 0 R /K 58 /ActualText (\(M1700, R&D\) in BALF supernatants or Lung ) /S /Span >> endobj 4288 0 obj << /Pg 5286 0 R /P 5247 0 R /K 59 /ActualText (homogenates,) /S /Span >> endobj 4289 0 obj << /Pg 5286 0 R /P 5247 0 R /K 60 /ActualText ( ) /S /Span >> endobj 4290 0 obj << /Pg 5286 0 R /P 5247 0 R /K 61 /ActualText (mouse) /S /Span >> endobj 4291 0 obj << /Pg 5286 0 R /P 5247 0 R /K 62 /ActualText ( ) /S /Span >> endobj 4292 0 obj << /Pg 5286 0 R /P 5247 0 R /K 63 /ActualText (cotinine \(EA100902, Or) /S /Span >> endobj 4293 0 obj << /Pg 5286 0 R /P 5247 0 R /K 64 /ActualText (iGene \) ) /S /Span >> endobj 4294 0 obj << /Pg 5286 0 R /P 5247 0 R /K 65 /ActualText (in) /S /Span >> endobj 4295 0 obj << /Pg 5286 0 R /P 5247 0 R /K 66 /ActualText ( ) /S /Span >> endobj 4296 0 obj << /Pg 5286 0 R /P 5247 0 R /K 67 /ActualText (serum) /S /Span >> endobj 4297 0 obj << /Pg 5286 0 R /P 5247 0 R /K 68 /ActualText (,) /S /Span >> endobj 4298 0 obj << /Pg 5286 0 R /P 5247 0 R /K 69 /ActualText ( ) /S /Span >> endobj 4299 0 obj << /Pg 5286 0 R /P 5247 0 R /K 70 /ActualText (human anti) /S /Span >> endobj 4300 0 obj << /Pg 5286 0 R /P 5247 0 R /K 71 /ActualText (-) /S /Span >> endobj 4301 0 obj << /Pg 5286 0 R /P 5247 0 R /K 72 /ActualText (elastin \(E01E0050, ) /S /Span >> endobj 4302 0 obj << /Pg 5286 0 R /P 5247 0 R /K 73 /ActualText (Bluegene\) in plasma, and IL17A \(BMS2017HS, Invitrogen\) in induced sputum were ) /S /Span >> endobj 4303 0 obj << /Pg 5286 0 R /P 5247 0 R /K 74 /ActualText (determined with ELISA kits following the manufacturer s protocol. ) /S /Span >> endobj 4304 0 obj << /Pg 5286 0 R /P 5247 0 R /K 75 /ActualText ( ) /S /Span >> endobj 4269 0 obj << /Pg 5286 0 R /P 5246 0 R /K 40 /ActualText (ELISA) /S /Span >> endobj 4270 0 obj << /Pg 5286 0 R /P 5246 0 R /K 41 /ActualText ( ) /S /Span >> endobj 4268 0 obj << /Pg 5286 0 R /P 5245 0 R /K 39 /ActualText ( ) /S /Span >> endobj 4229 0 obj << /Pg 5286 0 R /P 5244 0 R /K 0 /ActualText (triplicate. PCR primers used are shown as below: \(Mouse ) /S /Span >> endobj 4230 0 obj << /Pg 5286 0 R /P 5244 0 R /K 1 /ActualText (Gapdh) /S /Span >> endobj 4231 0 obj << /Pg 5286 0 R /P 5244 0 R /K 2 /ActualText (: Forword: ) /S /Span >> endobj 4232 0 obj << /Pg 5286 0 R /P 5244 0 R /K 3 /ActualText (5 ) /S /Span >> endobj 4233 0 obj << /Pg 5286 0 R /P 5244 0 R /K 4 /ActualText (-) /S /Span >> endobj 4234 0 obj << /Pg 5286 0 R /P 5244 0 R /K 5 /ActualText (AGGTCGGTGTGAACGGATTTG) /S /Span >> endobj 4235 0 obj << /Pg 5286 0 R /P 5244 0 R /K 6 /ActualText (-) /S /Span >> endobj 4236 0 obj << /Pg 5286 0 R /P 5244 0 R /K 7 /ActualText (3 , Reverse: 5 ) /S /Span >> endobj 4237 0 obj << /Pg 5286 0 R /P 5244 0 R /K 8 /ActualText (-) /S /Span >> endobj 4238 0 obj << /Pg 5286 0 R /P 5244 0 R /K 9 /ActualText (GGGGTCGTTGATGGCAACA) /S /Span >> endobj 4239 0 obj << /Pg 5286 0 R /P 5244 0 R /K 10 /ActualText (-) /S /Span >> endobj 4240 0 obj << /Pg 5286 0 R /P 5244 0 R /K 11 /ActualText (3 ; ) /S /Span >> endobj 4241 0 obj << /Pg 5286 0 R /P 5244 0 R /K 12 /ActualText (Mouse ) /S /Span >> endobj 4242 0 obj << /Pg 5286 0 R /P 5244 0 R /K 13 /ActualText (Muc5ac) /S /Span >> endobj 4243 0 obj << /Pg 5286 0 R /P 5244 0 R /K 14 /ActualText (: Forword: 5 ) /S /Span >> endobj 4244 0 obj << /Pg 5286 0 R /P 5244 0 R /K 15 /ActualText (-) /S /Span >> endobj 4245 0 obj << /Pg 5286 0 R /P 5244 0 R /K 16 /ActualText (CTGTGACATTATCCCATAAGCCC) /S /Span >> endobj 4246 0 obj << /Pg 5286 0 R /P 5244 0 R /K 17 /ActualText (-) /S /Span >> endobj 4247 0 obj << /Pg 5286 0 R /P 5244 0 R /K 18 /ActualText (3 , Reverse: ) /S /Span >> endobj 4248 0 obj << /Pg 5286 0 R /P 5244 0 R /K 19 /ActualText (5 ) /S /Span >> endobj 4249 0 obj << /Pg 5286 0 R /P 5244 0 R /K 20 /ActualText (-) /S /Span >> endobj 4250 0 obj << /Pg 5286 0 R /P 5244 0 R /K 21 /ActualText (AAGGGGTATAGCTGGCCTGA) /S /Span >> endobj 4251 0 obj << /Pg 5286 0 R /P 5244 0 R /K 22 /ActualText (-) /S /Span >> endobj 4252 0 obj << /Pg 5286 0 R /P 5244 0 R /K 23 /ActualText (3 ; Mouse ) /S /Span >> endobj 4253 0 obj << /Pg 5286 0 R /P 5244 0 R /K 24 /ActualText (M) /S /Span >> endobj 4254 0 obj << /Pg 5286 0 R /P 5244 0 R /K 25 /ActualText (m) /S /Span >> endobj 4255 0 obj << /Pg 5286 0 R /P 5244 0 R /K 26 /ActualText (p12) /S /Span >> endobj 4256 0 obj << /Pg 5286 0 R /P 5244 0 R /K 27 /ActualText (: Forword: ) /S /Span >> endobj 4257 0 obj << /Pg 5286 0 R /P 5244 0 R /K 28 /ActualText (5 ) /S /Span >> endobj 4258 0 obj << /Pg 5286 0 R /P 5244 0 R /K 29 /ActualText (-) /S /Span >> endobj 4259 0 obj << /Pg 5286 0 R /P 5244 0 R /K 30 /ActualText (CT) /S /Span >> endobj 4260 0 obj << /Pg 5286 0 R /P 5244 0 R /K 31 /ActualText (GCCCCATGAATGACAGTG) /S /Span >> endobj 4261 0 obj << /Pg 5286 0 R /P 5244 0 R /K 32 /ActualText (-) /S /Span >> endobj 4262 0 obj << /Pg 5286 0 R /P 5244 0 R /K 33 /ActualText (3 , Reverse: 5`) /S /Span >> endobj 4263 0 obj << /Pg 5286 0 R /P 5244 0 R /K 34 /ActualText (-) /S /Span >> endobj 4264 0 obj << /Pg 5286 0 R /P 5244 0 R /K 35 /ActualText (AGTTGCTTCTAAAC) /S /Span >> endobj 4265 0 obj << /Pg 5286 0 R /P 5244 0 R /K 36 /ActualText (-) /S /Span >> endobj 4266 0 obj << /Pg 5286 0 R /P 5244 0 R /K 37 /ActualText (3 \)) /S /Span >> endobj 4267 0 obj << /Pg 5286 0 R /P 5244 0 R /K 38 /ActualText ( ) /S /Span >> endobj 4209 0 obj << /Pg 5285 0 R /P 5243 0 R /K 74 /ActualText (RNA from BALF cells and lung homogenates was isolated with Trizol ) /S /Span >> endobj 4210 0 obj << /Pg 5285 0 R /P 5243 0 R /K 75 /ActualText (\(Invitrogen) /S /Span >> endobj 4211 0 obj << /Pg 5285 0 R /P 5243 0 R /K 76 /ActualText (, ) /S /Span >> endobj 4212 0 obj << /Pg 5285 0 R /P 5243 0 R /K 77 /ActualText (15596026\). Reverse transcription was performed with Reverse Transcription Reagents ) /S /Span >> endobj 4213 0 obj << /Pg 5285 0 R /P 5243 0 R /K 78 /ActualText (\(Takara Biotechnology, DRR037A\). Real) /S /Span >> endobj 4214 0 obj << /Pg 5285 0 R /P 5243 0 R /K 79 /ActualText (-) /S /Span >> endobj 4215 0 obj << /Pg 5285 0 R /P 5243 0 R /K 80 /ActualText (time RT) /S /Span >> endobj 4216 0 obj << /Pg 5285 0 R /P 5243 0 R /K 81 /ActualText (-) /S /Span >> endobj 4217 0 obj << /Pg 5285 0 R /P 5243 0 R /K 82 /ActualText (PCR was performed using the ) /S /Span >> endobj 4218 0 obj << /Pg 5285 0 R /P 5243 0 R /K 83 /ActualText (Ste) /S /Span >> endobj 4219 0 obj << /Pg 5285 0 R /P 5243 0 R /K 84 /ActualText (pOnePlus PCR system and gene expression assays \(Applied Biosystems, Foster City, CA, ) /S /Span >> endobj 4220 0 obj << /Pg 5285 0 R /P 5243 0 R /K 85 /ActualText (USA\). A ) /S /Span >> endobj 4221 0 obj << /Pg 5285 0 R /P 5243 0 R /K 86 /ActualText () /S /Span >> endobj 4222 0 obj << /Pg 5285 0 R /P 5243 0 R /K 87 /ActualText (Ct method was used to quantify mRNA levels for ) /S /Span >> endobj 4223 0 obj << /Pg 5285 0 R /P 5243 0 R /K 88 /ActualText (Muc5ac) /S /Span >> endobj 4224 0 obj << /Pg 5285 0 R /P 5243 0 R /K 89 /ActualText ( ) /S /Span >> endobj 4225 0 obj << /Pg 5285 0 R /P 5243 0 R /K 90 /ActualText (and ) /S /Span >> endobj 4226 0 obj << /Pg 5285 0 R /P 5243 0 R /K 91 /ActualText (Mmp12) /S /Span >> endobj 4227 0 obj << /Pg 5285 0 R /P 5243 0 R /K 92 /ActualText (. Gene ) /S /Span >> endobj 4228 0 obj << /Pg 5285 0 R /P 5243 0 R /K 93 /ActualText (expression was quantified using ABI and MS Excel software. Each sample was assayed in ) /S /Span >> endobj 4204 0 obj << /Pg 5285 0 R /P 5242 0 R /K 69 /ActualText (Quantitati) /S /Span >> endobj 4205 0 obj << /Pg 5285 0 R /P 5242 0 R /K 70 /ActualText (ve real) /S /Span >> endobj 4206 0 obj << /Pg 5285 0 R /P 5242 0 R /K 71 /ActualText (-) /S /Span >> endobj 4207 0 obj << /Pg 5285 0 R /P 5242 0 R /K 72 /ActualText (time PCR) /S /Span >> endobj 4208 0 obj << /Pg 5285 0 R /P 5242 0 R /K 73 /ActualText ( ) /S /Span >> endobj 4203 0 obj << /Pg 5285 0 R /P 5241 0 R /K 68 /ActualText ( ) /S /Span >> endobj 4178 0 obj << /Pg 5285 0 R /P 5240 0 R /K 43 /ActualText (Forty) /S /Span >> endobj 4179 0 obj << /Pg 5285 0 R /P 5240 0 R /K 44 /ActualText (-) /S /Span >> endobj 4180 0 obj << /Pg 5285 0 R /P 5240 0 R /K 45 /ActualText (eight hours after the last exposure to elastin, mice were sacrificed using an overdose of ) /S /Span >> endobj 4181 0 obj << /Pg 5285 0 R /P 5240 0 R /K 46 /ActualText (2) /S /Span >> endobj 4182 0 obj << /Pg 5285 0 R /P 5240 0 R /K 47 /ActualText (% pentobarbital sodium and tracheotomy was performed. ) /S /Span >> endobj 4183 0 obj << /Pg 5285 0 R /P 5240 0 R /K 48 /ActualText (Each mo) /S /Span >> endobj 4184 0 obj << /Pg 5285 0 R /P 5240 0 R /K 49 /ActualText (use ) /S /Span >> endobj 4185 0 obj << /Pg 5285 0 R /P 5240 0 R /K 50 /ActualText (was) /S /Span >> endobj 4186 0 obj << /Pg 5285 0 R /P 5240 0 R /K 51 /ActualText ( ) /S /Span >> endobj 4187 0 obj << /Pg 5285 0 R /P 5240 0 R /K 52 /ActualText (lavaged with 0.4 ) /S /Span >> endobj 4188 0 obj << /Pg 5285 0 R /P 5240 0 R /K 53 /ActualText (ml PBS) /S /Span >> endobj 4189 0 obj << /Pg 5285 0 R /P 5240 0 R /K 54 /ActualText ( ) /S /Span >> endobj 4190 0 obj << /Pg 5285 0 R /P 5240 0 R /K 55 /ActualText (by injecting into the lungs and drawing to collect cells for 3 times) /S /Span >> endobj 4191 0 obj << /Pg 5285 0 R /P 5240 0 R /K 56 /ActualText (. The total number ) /S /Span >> endobj 4192 0 obj << /Pg 5285 0 R /P 5240 0 R /K 57 /ActualText (of BALF cells was counted, then the remaining BALF was centrifuged \(400 g for 10min at ) /S /Span >> endobj 4193 0 obj << /Pg 5285 0 R /P 5240 0 R /K 58 /ActualText (4C\). The supernatant was retained for further ana) /S /Span >> endobj 4194 0 obj << /Pg 5285 0 R /P 5240 0 R /K 59 /ActualText (lysis, while the cell pellet was resuspended ) /S /Span >> endobj 4195 0 obj << /Pg 5285 0 R /P 5240 0 R /K 60 /ActualText (in PBS moderately and centrifuged on glass slides. Then cells on glass slides were stained ) /S /Span >> endobj 4196 0 obj << /Pg 5285 0 R /P 5240 0 R /K 61 /ActualText (with Wright) /S /Span >> endobj 4197 0 obj << /Pg 5285 0 R /P 5240 0 R /K 62 /ActualText ( ) /S /Span >> endobj 4198 0 obj << /Pg 5285 0 R /P 5240 0 R /K 63 /ActualText (Giemsa stain \(Baso, BA) /S /Span >> endobj 4199 0 obj << /Pg 5285 0 R /P 5240 0 R /K 64 /ActualText (-) /S /Span >> endobj 4200 0 obj << /Pg 5285 0 R /P 5240 0 R /K 65 /ActualText (4017\), and differential counts were assessed by ) /S /Span >> endobj 4201 0 obj << /Pg 5285 0 R /P 5240 0 R /K 66 /ActualText (counting 200 total cells.) /S /Span >> endobj 4202 0 obj << /Pg 5285 0 R /P 5240 0 R /K 67 /ActualText ( ) /S /Span >> endobj 4176 0 obj << /Pg 5285 0 R /P 5239 0 R /K 41 /ActualText (BALF collection ) /S /Span >> endobj 4177 0 obj << /Pg 5285 0 R /P 5239 0 R /K 42 /ActualText ( ) /S /Span >> endobj 4175 0 obj << /Pg 5285 0 R /P 5238 0 R /K 40 /ActualText ( ) /S /Span >> endobj 4143 0 obj << /Pg 5285 0 R /P 5237 0 R /K 8 /ActualText ( ) /S /Span >> endobj 4144 0 obj << /Pg 5285 0 R /P 5237 0 R /K 9 /ActualText ( ) /S /Span >> endobj 4145 0 obj << /Pg 5285 0 R /P 5237 0 R /K 10 /ActualText (Mouse mediastinal lymph nodes from air) /S /Span >> endobj 4146 0 obj << /Pg 5285 0 R /P 5237 0 R /K 11 /ActualText (-) /S /Span >> endobj 4147 0 obj << /Pg 5285 0 R /P 5237 0 R /K 12 /ActualText ( ) /S /Span >> endobj 4148 0 obj << /Pg 5285 0 R /P 5237 0 R /K 13 /ActualText (or CS) /S /Span >> endobj 4149 0 obj << /Pg 5285 0 R /P 5237 0 R /K 14 /ActualText (-) /S /Span >> endobj 4150 0 obj << /Pg 5285 0 R /P 5237 0 R /K 15 /ActualText ( ) /S /Span >> endobj 4151 0 obj << /Pg 5285 0 R /P 5237 0 R /K 16 /ActualText (sensitised C57BL/6 mice were harvested ) /S /Span >> endobj 4152 0 obj << /Pg 5285 0 R /P 5237 0 R /K 17 /ActualText (after euthanasia, and single) /S /Span >> endobj 4153 0 obj << /Pg 5285 0 R /P 5237 0 R /K 18 /ActualText (-) /S /Span >> endobj 4154 0 obj << /Pg 5285 0 R /P 5237 0 R /K 19 /ActualText (cell suspensions were prepared using sterile syringe plunger. Cells ) /S /Span >> endobj 4155 0 obj << /Pg 5285 0 R /P 5237 0 R /K 20 /ActualText (were finally strained through a 40) /S /Span >> endobj 4156 0 obj << /Pg 5285 0 R /P 5237 0 R /K 21 /ActualText (-) /S /Span >> endobj 4157 0 obj << /Pg 5285 0 R /P 5237 0 R /K 22 /ActualText (m) /S /Span >> endobj 4158 0 obj << /Pg 5285 0 R /P 5237 0 R /K 23 /ActualText (m filter. CD3) /S /Span >> endobj 4159 0 obj << /Pg 5285 0 R /P 5237 0 R /K 24 /ActualText (+) /S /Span >> endobj 4160 0 obj << /Pg 5285 0 R /P 5237 0 R /K 25 /ActualText ( ) /S /Span >> endobj 4161 0 obj << /Pg 5285 0 R /P 5237 0 R /K 26 /ActualText (T cells were purified with Mojosort!" ) /S /Span >> endobj 4162 0 obj << /Pg 5285 0 R /P 5237 0 R /K 27 /ActualText (mouse CD3 T cell isolation kit \(Biolegend, 480024\) according to the manufacturer s protocol ) /S /Span >> endobj 4163 0 obj << /Pg 5285 0 R /P 5237 0 R /K 28 /ActualText (and were delivered \(1x10) /S /Span >> endobj 4164 0 obj << /Pg 5285 0 R /P 5237 0 R /K 29 /ActualText (6) /S /Span >> endobj 4165 0 obj << /Pg 5285 0 R /P 5237 0 R /K 30 /ActualText ( ) /S /Span >> endobj 4166 0 obj << /Pg 5285 0 R /P 5237 0 R /K 31 /ActualText (cells/mouse\) intratracheally in 50 ) /S /Span >> endobj 4167 0 obj << /Pg 5285 0 R /P 5237 0 R /K 32 /ActualText (m) /S /Span >> endobj 4168 0 obj << /Pg 5285 0 R /P 5237 0 R /K 33 /ActualText (l PBS to nave recipient under ) /S /Span >> endobj 4169 0 obj << /Pg 5285 0 R /P 5237 0 R /K 34 /ActualText (anaesthesia. 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183 0 R] /S /P >> endobj 5376 0 obj << /Pg 5343 0 R /P 40 0 R /K [184 0 R 185 0 R 186 0 R 187 0 R 188 0 R 189 0 R] /S /P >> endobj 5377 0 obj << /Pg 5343 0 R /P 40 0 R /K [190 0 R 191 0 R 192 0 R 193 0 R 194 0 R 195 0 R 196 0 R 197 0 R] /S /P >> endobj 5378 0 obj << /Pg 5343 0 R /P 40 0 R /K [198 0 R 199 0 R 200 0 R 201 0 R 202 0 R 203 0 R 204 0 R] /S /P >> endobj 5379 0 obj << /Pg 5343 0 R /P 40 0 R /K [205 0 R 206 0 R 207 0 R 208 0 R] /S /P >> endobj 5380 0 obj << /Pg 5343 0 R /P 40 0 R /K [209 0 R 210 0 R 211 0 R 212 0 R] /S /P >> endobj 5381 0 obj << /Pg 5343 0 R /P 40 0 R /K [213 0 R 214 0 R 215 0 R 216 0 R 217 0 R 218 0 R 219 0 R 220 0 R 221 0 R] /S /P >> endobj 5382 0 obj << /Pg 5343 0 R /P 40 0 R /K [222 0 R 223 0 R 224 0 R 225 0 R 226 0 R 227 0 R 228 0 R 229 0 R 230 0 R 231 0 R 232 0 R 233 0 R 234 0 R] /S /P >> endobj 5383 0 obj << /Pg 5343 0 R /P 40 0 R /K [235 0 R 236 0 R 237 0 R 238 0 R 239 0 R 240 0 R 241 0 R 242 0 R 243 0 R 244 0 R] /S /P >> endobj 5384 0 obj << /Pg 5039 0 R /P 40 0 R /K [245 0 R 246 0 R 247 0 R] /S /P >> endobj 5385 0 obj << /Pg 5039 0 R /P 40 0 R /K [248 0 R 249 0 R 250 0 R 251 0 R] /S /P >> endobj 5386 0 obj << /Pg 5039 0 R /P 40 0 R /K [252 0 R 253 0 R 254 0 R 255 0 R] /S /P >> endobj 5387 0 obj << /Pg 5039 0 R /P 40 0 R /K 271 0 R /S /P >> endobj 5388 0 obj << /Pg 5039 0 R /P 40 0 R /K 272 0 R /S /P >> endobj 5389 0 obj << /Pg 5039 0 R /P 40 0 R /K [273 0 R 274 0 R 275 0 R] /S /P >> endobj 5390 0 obj << /Pg 5039 0 R /P 40 0 R /K [276 0 R 277 0 R 278 0 R 279 0 R 280 0 R 281 0 R 282 0 R 283 0 R 284 0 R 285 0 R 286 0 R 287 0 R 288 0 R 289 0 R 290 0 R 291 0 R 292 0 R 293 0 R 294 0 R 295 0 R] /S /P >> endobj 5391 0 obj << /Pg 5039 0 R /P 40 0 R /K 296 0 R /S /P >> endobj 5392 0 obj << /Pg 5039 0 R /P 40 0 R /K 297 0 R /S /P >> endobj 5393 0 obj << /Pg 5039 0 R /P 40 0 R /K [298 0 R 299 0 R 300 0 R] /S /P >> endobj 5394 0 obj << /Pg 5039 0 R /P 40 0 R /K [301 0 R 302 0 R 303 0 R 304 0 R 305 0 R 306 0 R 307 0 R 308 0 R 309 0 R 310 0 R 311 0 R 312 0 R 313 0 R 314 0 R 315 0 R 316 0 R 317 0 R 318 0 R 319 0 R 320 0 R 321 0 R 322 0 R 323 0 R 324 0 R 325 0 R 326 0 R 327 0 R] /S /P >> endobj 5395 0 obj << /Pg 5039 0 R /P 40 0 R /K 328 0 R /S /P >> endobj 5396 0 obj << /Pg 5039 0 R /P 40 0 R /K 329 0 R /S /P >> endobj 5397 0 obj << /Pg 5039 0 R /P 40 0 R /K 330 0 R /S /P >> endobj 5398 0 obj << /Pg 5039 0 R /P 40 0 R /K 331 0 R /S /P >> endobj 5399 0 obj << /Pg 5039 0 R /P 40 0 R /K [332 0 R 333 0 R] /S /P >> endobj 5400 0 obj << /Pg 5344 0 R /P 40 0 R /K [334 0 R 335 0 R] /S /P >> endobj 5401 0 obj << /Pg 5344 0 R /P 40 0 R /K [336 0 R 337 0 R 338 0 R 339 0 R 340 0 R 341 0 R 342 0 R 343 0 R 344 0 R 345 0 R 346 0 R 347 0 R] /S /P >> endobj 5402 0 obj << /Pg 5344 0 R /P 40 0 R /K [348 0 R 349 0 R 350 0 R 351 0 R 352 0 R 353 0 R 354 0 R 355 0 R 356 0 R 357 0 R 358 0 R 359 0 R 360 0 R 361 0 R 362 0 R 363 0 R 364 0 R 365 0 R 366 0 R 367 0 R 368 0 R 369 0 R 370 0 R 371 0 R 372 0 R 373 0 R 374 0 R 375 0 R 376 0 R 377 0 R 378 0 R 379 0 R 380 0 R 381 0 R 382 0 R 383 0 R 384 0 R 385 0 R 386 0 R 387 0 R 388 0 R 389 0 R 390 0 R 391 0 R 392 0 R 393 0 R 394 0 R 395 0 R 396 0 R 397 0 R 398 0 R 399 0 R 400 0 R 401 0 R 402 0 R 403 0 R 404 0 R 405 0 R 406 0 R] /S /P >> endobj 5403 0 obj << /Pg 5344 0 R /P 40 0 R /K [407 0 R 408 0 R 409 0 R 410 0 R 411 0 R 412 0 R 413 0 R 414 0 R 415 0 R 416 0 R 417 0 R 418 0 R 419 0 R 420 0 R 421 0 R 422 0 R 423 0 R 424 0 R 425 0 R 426 0 R 427 0 R 428 0 R 429 0 R 430 0 R 431 0 R 432 0 R 433 0 R 434 0 R 435 0 R 436 0 R 437 0 R 438 0 R 439 0 R 440 0 R 441 0 R 442 0 R 443 0 R 444 0 R 445 0 R 446 0 R 447 0 R 448 0 R 449 0 R 450 0 R 451 0 R 452 0 R 453 0 R 454 0 R 455 0 R 456 0 R 457 0 R 458 0 R 459 0 R 460 0 R 461 0 R 462 0 R 463 0 R 464 0 R 465 0 R 466 0 R 467 0 R 468 0 R 469 0 R 470 0 R 471 0 R 472 0 R 473 0 R 474 0 R 475 0 R 476 0 R 477 0 R 478 0 R 479 0 R 480 0 R] /S /P >> endobj 5404 0 obj << /Pg 5344 0 R /P 40 0 R /K [481 0 R 482 0 R 483 0 R 484 0 R 485 0 R 486 0 R 487 0 R 488 0 R 489 0 R 490 0 R 491 0 R 492 0 R 493 0 R 494 0 R 495 0 R 496 0 R 497 0 R 498 0 R 499 0 R 500 0 R 501 0 R 502 0 R] /S /P >> endobj 5405 0 obj << /Pg 5344 0 R /P 40 0 R /K 503 0 R /S /P >> endobj 5406 0 obj << /Pg 5344 0 R /P 40 0 R /K 504 0 R /S /P >> endobj 5407 0 obj << /Pg 5344 0 R /P 40 0 R /K [505 0 R 506 0 R] /S /P >> endobj 5408 0 obj << /Pg 5345 0 R /P 40 0 R /K [507 0 R 508 0 R] /S /P >> endobj 5409 0 obj << /Pg 5345 0 R /P 40 0 R /K [509 0 R 510 0 R 511 0 R 512 0 R 513 0 R 514 0 R 515 0 R 516 0 R 517 0 R 518 0 R 519 0 R 520 0 R 521 0 R 522 0 R 523 0 R 524 0 R 525 0 R 526 0 R 527 0 R 528 0 R 529 0 R 530 0 R 531 0 R 532 0 R 533 0 R 534 0 R 535 0 R 536 0 R 537 0 R 538 0 R 539 0 R 540 0 R 541 0 R 542 0 R 543 0 R 544 0 R 545 0 R 546 0 R 547 0 R 548 0 R 549 0 R 550 0 R 551 0 R 552 0 R 553 0 R 554 0 R 555 0 R 556 0 R 557 0 R] /S /P >> endobj 5410 0 obj << /Pg 5345 0 R /P 40 0 R /K [558 0 R 559 0 R 560 0 R 561 0 R 562 0 R 563 0 R 564 0 R 565 0 R 566 0 R 567 0 R 568 0 R 569 0 R 570 0 R 571 0 R 572 0 R 573 0 R 574 0 R 575 0 R 576 0 R 577 0 R 578 0 R 579 0 R 580 0 R 581 0 R 582 0 R 583 0 R 584 0 R 585 0 R 586 0 R 587 0 R 588 0 R 589 0 R 590 0 R 591 0 R 592 0 R 593 0 R 594 0 R 595 0 R 596 0 R 597 0 R 598 0 R 599 0 R 600 0 R 601 0 R 602 0 R 603 0 R 604 0 R 605 0 R 606 0 R 607 0 R 608 0 R 609 0 R 610 0 R 611 0 R 612 0 R 613 0 R 614 0 R 615 0 R 616 0 R 617 0 R 618 0 R 619 0 R 620 0 R 621 0 R 622 0 R 623 0 R 624 0 R 625 0 R 626 0 R 627 0 R 628 0 R 629 0 R 630 0 R 631 0 R 632 0 R 633 0 R 634 0 R 635 0 R 636 0 R 637 0 R 638 0 R 639 0 R 640 0 R 641 0 R 642 0 R 643 0 R 644 0 R 645 0 R 646 0 R 647 0 R 648 0 R 649 0 R 650 0 R 651 0 R 652 0 R 653 0 R 654 0 R 655 0 R 656 0 R 657 0 R 658 0 R 659 0 R 660 0 R] /S /P >> endobj 5411 0 obj << /Pg 5345 0 R /P 40 0 R /K [661 0 R 662 0 R 663 0 R 664 0 R 665 0 R 666 0 R 667 0 R 668 0 R 669 0 R 670 0 R 671 0 R 672 0 R 673 0 R 674 0 R 675 0 R 676 0 R 677 0 R 678 0 R 679 0 R 680 0 R 681 0 R 682 0 R 683 0 R 684 0 R 685 0 R 686 0 R 687 0 R 688 0 R 689 0 R 690 0 R 691 0 R 692 0 R 693 0 R 694 0 R 695 0 R 696 0 R 697 0 R 698 0 R 699 0 R 700 0 R 701 0 R 702 0 R 703 0 R 704 0 R 705 0 R 706 0 R 707 0 R 708 0 R 709 0 R 710 0 R 711 0 R 712 0 R 713 0 R 714 0 R 715 0 R 716 0 R 717 0 R 718 0 R 719 0 R 720 0 R 721 0 R 722 0 R 723 0 R 724 0 R 725 0 R 726 0 R 727 0 R 728 0 R] /S /P >> endobj 5412 0 obj << /Pg 5345 0 R /P 40 0 R /K 729 0 R /S /P >> endobj 5413 0 obj << /Pg 5345 0 R /P 40 0 R /K 730 0 R /S /P >> endobj 5414 0 obj << /Pg 5345 0 R /P 40 0 R /K [731 0 R 732 0 R] /S /P >> endobj 5415 0 obj << /Pg 5346 0 R /P 40 0 R /K [733 0 R 734 0 R 735 0 R] /S /P >> endobj 5416 0 obj << /Pg 5346 0 R /P 40 0 R /K [736 0 R 737 0 R] /S /P >> endobj 5417 0 obj << /Pg 5346 0 R /P 40 0 R /K 738 0 R /S /P >> endobj 5418 0 obj << /Pg 5346 0 R /P 40 0 R /K [739 0 R 740 0 R] /S /P >> endobj 5419 0 obj << /Pg 5346 0 R /P 40 0 R /K [741 0 R 742 0 R 743 0 R 744 0 R 745 0 R 746 0 R 747 0 R 748 0 R 749 0 R 750 0 R 751 0 R 752 0 R 753 0 R 754 0 R 755 0 R 756 0 R 757 0 R 758 0 R 759 0 R 760 0 R 761 0 R 762 0 R 763 0 R 764 0 R 765 0 R 766 0 R 767 0 R 768 0 R 769 0 R 770 0 R 771 0 R 772 0 R 773 0 R 774 0 R 775 0 R 776 0 R 777 0 R 778 0 R] /S /P >> endobj 5420 0 obj << /Pg 5346 0 R /P 40 0 R /K 779 0 R /S /P >> endobj 5421 0 obj << /Pg 5346 0 R /P 40 0 R /K [780 0 R 781 0 R] /S /P >> endobj 5422 0 obj << /Pg 5346 0 R /P 40 0 R /K [782 0 R 783 0 R 784 0 R 785 0 R 786 0 R 787 0 R 788 0 R 789 0 R 790 0 R 791 0 R 792 0 R 793 0 R 794 0 R 795 0 R 796 0 R 797 0 R 798 0 R 799 0 R 800 0 R 801 0 R 802 0 R 803 0 R 804 0 R 805 0 R 806 0 R 807 0 R 808 0 R 809 0 R 810 0 R 811 0 R 812 0 R 813 0 R 814 0 R 815 0 R 816 0 R 817 0 R 818 0 R 819 0 R 820 0 R 821 0 R 822 0 R 823 0 R 824 0 R 825 0 R 826 0 R] /S /P >> endobj 5423 0 obj << /Pg 5346 0 R /P 40 0 R /K [827 0 R 828 0 R 829 0 R 830 0 R 831 0 R 832 0 R 833 0 R 834 0 R 835 0 R 836 0 R 837 0 R 838 0 R 839 0 R 840 0 R 841 0 R 842 0 R 843 0 R 844 0 R 845 0 R 846 0 R 847 0 R 848 0 R 849 0 R 850 0 R 851 0 R 852 0 R 853 0 R 854 0 R 855 0 R 856 0 R 857 0 R 858 0 R 859 0 R 860 0 R 861 0 R 862 0 R 863 0 R 864 0 R 865 0 R 866 0 R 867 0 R 868 0 R 869 0 R 870 0 R 871 0 R 872 0 R 873 0 R 874 0 R 875 0 R 876 0 R 877 0 R 878 0 R 879 0 R 880 0 R 881 0 R 882 0 R 883 0 R 884 0 R 885 0 R 886 0 R 887 0 R 888 0 R 889 0 R 890 0 R 891 0 R 892 0 R 893 0 R] /S /P >> endobj 5424 0 obj << /Pg 5346 0 R /P 40 0 R /K [894 0 R 895 0 R 896 0 R 897 0 R 898 0 R 899 0 R 900 0 R 901 0 R 902 0 R 903 0 R 904 0 R 905 0 R 906 0 R 907 0 R 908 0 R 909 0 R 910 0 R 911 0 R 912 0 R 913 0 R 914 0 R 915 0 R 916 0 R 917 0 R 918 0 R] /S /P >> endobj 5425 0 obj << /Pg 5346 0 R /P 40 0 R /K 919 0 R /S /P >> endobj 5426 0 obj << /Pg 5346 0 R /P 40 0 R /K [920 0 R 921 0 R] /S /P >> endobj 5427 0 obj << /Pg 5346 0 R /P 40 0 R /K [922 0 R 923 0 R 924 0 R] /S /P >> endobj 5428 0 obj << /Pg 5347 0 R /P 40 0 R /K [925 0 R 926 0 R 927 0 R 928 0 R 929 0 R 930 0 R 931 0 R 932 0 R 933 0 R 934 0 R 935 0 R 936 0 R 937 0 R 938 0 R 939 0 R] /S /P >> endobj 5429 0 obj << /Pg 5347 0 R /P 40 0 R /K 940 0 R /S /P >> endobj 5430 0 obj << /Pg 5347 0 R /P 40 0 R /K [941 0 R 942 0 R 943 0 R 944 0 R 945 0 R 946 0 R 947 0 R] /S /P >> endobj 5431 0 obj << /Pg 5347 0 R /P 40 0 R /K [5561 0 R 5562 0 R 5563 0 R 5564 0 R 5565 0 R 5566 0 R 5567 0 R 5568 0 R 5569 0 R 5570 0 R 5571 0 R 5572 0 R 5573 0 R 5574 0 R 5575 0 R 5576 0 R 5577 0 R 5578 0 R 5579 0 R 5580 0 R] /S /Table >> endobj 5432 0 obj << /Pg 5347 0 R /P 40 0 R /K [948 0 R 949 0 R 950 0 R 951 0 R 952 0 R 953 0 R 954 0 R 955 0 R 956 0 R 957 0 R 958 0 R 959 0 R 960 0 R 961 0 R 962 0 R 963 0 R 964 0 R 965 0 R 966 0 R 967 0 R 968 0 R 969 0 R 970 0 R 971 0 R 972 0 R 973 0 R 974 0 R 975 0 R 976 0 R 977 0 R 978 0 R 979 0 R 980 0 R 981 0 R 982 0 R 983 0 R 984 0 R 985 0 R 986 0 R 987 0 R 988 0 R 989 0 R 990 0 R 991 0 R 992 0 R 993 0 R] /S /P >> endobj 5433 0 obj << /Pg 5348 0 R /P 40 0 R /K [1266 0 R 1267 0 R] /S /P >> endobj 5434 0 obj << /Pg 5348 0 R /P 40 0 R /K [1268 0 R 1269 0 R 1270 0 R 1271 0 R 1272 0 R 1273 0 R 1274 0 R 1275 0 R 1276 0 R 1277 0 R 1278 0 R 1279 0 R 1280 0 R 1281 0 R 1282 0 R 1283 0 R] /S /P >> endobj 5435 0 obj << /Pg 5348 0 R /P 40 0 R /K [1284 0 R 1285 0 R 1286 0 R 1287 0 R 1288 0 R 1289 0 R 1290 0 R 1291 0 R 1292 0 R 1293 0 R 1294 0 R 1295 0 R 1296 0 R 1297 0 R 1298 0 R 1299 0 R 1300 0 R 1301 0 R 1302 0 R 1303 0 R 1304 0 R 1305 0 R 1306 0 R 1307 0 R 1308 0 R 1309 0 R 1310 0 R 1311 0 R 1312 0 R 1313 0 R 1314 0 R 1315 0 R 1316 0 R 1317 0 R 1318 0 R 1319 0 R 1320 0 R 1321 0 R 1322 0 R 1323 0 R 1324 0 R 1325 0 R 1326 0 R 1327 0 R 1328 0 R 1329 0 R 1330 0 R 1331 0 R 1332 0 R 1333 0 R 1334 0 R 1335 0 R 1336 0 R 1337 0 R 1338 0 R 1339 0 R 1340 0 R 1341 0 R 1342 0 R 1343 0 R 1344 0 R 1345 0 R] /S /P >> endobj 5436 0 obj << /Pg 5348 0 R /P 40 0 R /K [1346 0 R 1347 0 R 1348 0 R 1349 0 R 1350 0 R 1351 0 R 1352 0 R 1353 0 R 1354 0 R 1355 0 R 1356 0 R 1357 0 R 1358 0 R 1359 0 R 1360 0 R 1361 0 R 1362 0 R 1363 0 R 1364 0 R 1365 0 R 1366 0 R 1367 0 R 1368 0 R 1369 0 R 1370 0 R 1371 0 R 1372 0 R 1373 0 R 1374 0 R 1375 0 R 1376 0 R 1377 0 R 1378 0 R 1379 0 R 1380 0 R 1381 0 R 1382 0 R 1383 0 R 1384 0 R 1385 0 R 1386 0 R 1387 0 R 1388 0 R 1389 0 R 1390 0 R 1391 0 R 1392 0 R 1393 0 R 1394 0 R 1395 0 R 1396 0 R 1397 0 R 1398 0 R 1399 0 R 1400 0 R 1401 0 R 1402 0 R 1403 0 R 1404 0 R 1405 0 R 1406 0 R 1407 0 R 1408 0 R 1409 0 R 1410 0 R 1411 0 R] /S /P >> endobj 5437 0 obj << /Pg 5348 0 R /P 40 0 R /K [1412 0 R 1413 0 R 1414 0 R 1415 0 R 1416 0 R 1417 0 R 1418 0 R 1419 0 R 1420 0 R 1421 0 R 1422 0 R 1423 0 R 1424 0 R 1425 0 R 1426 0 R 1427 0 R 1428 0 R 1429 0 R 1430 0 R 1431 0 R 1432 0 R 1433 0 R 1434 0 R 1435 0 R 1436 0 R 1437 0 R 1438 0 R 1439 0 R 1440 0 R 1441 0 R 1442 0 R 1443 0 R] /S /P >> endobj 5438 0 obj << /Pg 5581 0 R /P 40 0 R /K [1444 0 R 1445 0 R 1446 0 R 1447 0 R 1448 0 R 1449 0 R 1450 0 R 1451 0 R 1452 0 R 1453 0 R 1454 0 R 1455 0 R] /S /P >> endobj 5439 0 obj << /Pg 5581 0 R /P 40 0 R /K 1456 0 R /S /P >> endobj 5440 0 obj << /Pg 5581 0 R /P 40 0 R /K [1457 0 R 1458 0 R 1459 0 R 1460 0 R 1461 0 R 1462 0 R 1463 0 R 1464 0 R 1465 0 R 1466 0 R 1467 0 R] /S /P >> endobj 5441 0 obj << /Pg 5581 0 R /P 40 0 R /K [1468 0 R 1469 0 R 1470 0 R 1471 0 R 1472 0 R 1473 0 R 1474 0 R 1475 0 R 1476 0 R 1477 0 R 1478 0 R 1479 0 R 1480 0 R 1481 0 R 1482 0 R 1483 0 R 1484 0 R 1485 0 R 1486 0 R 1487 0 R 1488 0 R 1489 0 R 1490 0 R 1491 0 R 1492 0 R 1493 0 R 1494 0 R 1495 0 R 1496 0 R 1497 0 R 1498 0 R 1499 0 R 1500 0 R 1501 0 R 1502 0 R 1503 0 R 1504 0 R 1505 0 R 1506 0 R 1507 0 R 1508 0 R 1509 0 R 1510 0 R 1511 0 R 1512 0 R 1513 0 R 1514 0 R 1515 0 R 1516 0 R 1517 0 R 1518 0 R 1519 0 R 1520 0 R 1521 0 R 1522 0 R 1523 0 R 1524 0 R 1525 0 R 1526 0 R 1527 0 R 1528 0 R 1529 0 R 1530 0 R 1531 0 R 1532 0 R 1533 0 R 1534 0 R 1535 0 R 1536 0 R 1537 0 R 1538 0 R 1539 0 R 1540 0 R 1541 0 R 1542 0 R 1543 0 R 1544 0 R] /S /P >> endobj 5442 0 obj << /Pg 5581 0 R /P 40 0 R /K 1545 0 R /S /P >> endobj 5443 0 obj << /Pg 5581 0 R /P 40 0 R /K [1546 0 R 1547 0 R 1548 0 R 1549 0 R 1550 0 R 1551 0 R 1552 0 R 1553 0 R 1554 0 R 1555 0 R 1556 0 R 1557 0 R 1558 0 R 1559 0 R] /S /P >> endobj 5444 0 obj << /Pg 5581 0 R /P 40 0 R /K [1560 0 R 1561 0 R 1562 0 R 1563 0 R 1564 0 R 1565 0 R 1566 0 R 1567 0 R 1568 0 R 1569 0 R 1570 0 R 1571 0 R 1572 0 R 1573 0 R 1574 0 R 1575 0 R 1576 0 R 1577 0 R 1578 0 R 1579 0 R 1580 0 R 1581 0 R 1582 0 R 1583 0 R 1584 0 R 1585 0 R 1586 0 R 1587 0 R 1588 0 R 1589 0 R 1590 0 R 1591 0 R 1592 0 R 1593 0 R 1594 0 R 1595 0 R 1596 0 R 1597 0 R 1598 0 R 1599 0 R 1600 0 R 1601 0 R 1602 0 R 1603 0 R 1604 0 R 1605 0 R 1606 0 R 1607 0 R 1608 0 R 1609 0 R 1610 0 R 1611 0 R] /S /P >> endobj 5445 0 obj << /Pg 5581 0 R /P 40 0 R /K [1612 0 R 1613 0 R 1614 0 R 1615 0 R 1616 0 R 1617 0 R 1618 0 R 1619 0 R 1620 0 R 1621 0 R 1622 0 R 1623 0 R 1624 0 R 1625 0 R 1626 0 R 1627 0 R 1628 0 R 1629 0 R 1630 0 R 1631 0 R 1632 0 R 1633 0 R 1634 0 R 1635 0 R 1636 0 R 1637 0 R 1638 0 R 1639 0 R 1640 0 R 1641 0 R 1642 0 R 1643 0 R 1644 0 R 1645 0 R 1646 0 R 1647 0 R 1648 0 R 1649 0 R 1650 0 R 1651 0 R 1652 0 R 1653 0 R 1654 0 R 1655 0 R 1656 0 R 1657 0 R 1658 0 R 1659 0 R 1660 0 R 1661 0 R 1662 0 R 1663 0 R 1664 0 R 1665 0 R 1666 0 R 1667 0 R 1668 0 R 1669 0 R 1670 0 R 1671 0 R 1672 0 R 1673 0 R 1674 0 R 1675 0 R 1676 0 R 1677 0 R 1678 0 R 1679 0 R 1680 0 R 1681 0 R 1682 0 R 1683 0 R 1684 0 R 1685 0 R 1686 0 R 1687 0 R] /S /P >> endobj 5446 0 obj << /Pg 5582 0 R /P 40 0 R /K [1688 0 R 1689 0 R 1690 0 R 1691 0 R 1692 0 R 1693 0 R 1694 0 R 1695 0 R 1696 0 R 1697 0 R 1698 0 R 1699 0 R 1700 0 R 1701 0 R 1702 0 R 1703 0 R 1704 0 R 1705 0 R 1706 0 R 1707 0 R 1708 0 R 1709 0 R 1710 0 R 1711 0 R 1712 0 R 1713 0 R 1714 0 R 1715 0 R 1716 0 R 1717 0 R 1718 0 R 1719 0 R 1720 0 R 1721 0 R 1722 0 R 1723 0 R 1724 0 R 1725 0 R 1726 0 R 1727 0 R 1728 0 R 1729 0 R 1730 0 R] /S /P >> endobj 5447 0 obj << /Pg 5582 0 R /P 40 0 R /K 1731 0 R /S /P >> endobj 5448 0 obj << /Pg 5582 0 R /P 40 0 R /K [1732 0 R 1733 0 R 1734 0 R] /S /P >> endobj 5449 0 obj << /Pg 5582 0 R /P 40 0 R /K [1735 0 R 1736 0 R 1737 0 R 1738 0 R 1739 0 R 1740 0 R 1741 0 R 1742 0 R 1743 0 R 1744 0 R 1745 0 R 1746 0 R 1747 0 R 1748 0 R 1749 0 R 1750 0 R 1751 0 R 1752 0 R 1753 0 R 1754 0 R 1755 0 R 1756 0 R 1757 0 R 1758 0 R 1759 0 R 1760 0 R 1761 0 R 1762 0 R 1763 0 R 1764 0 R 1765 0 R 1766 0 R 1767 0 R 1768 0 R 1769 0 R 1770 0 R 1771 0 R 1772 0 R 1773 0 R 1774 0 R 1775 0 R 1776 0 R 1777 0 R 1778 0 R 1779 0 R 1780 0 R 1781 0 R 1782 0 R 1783 0 R 1784 0 R 1785 0 R 1786 0 R 1787 0 R 1788 0 R 1789 0 R 1790 0 R 1791 0 R 1792 0 R 1793 0 R 1794 0 R 1795 0 R] /S /P >> endobj 5450 0 obj << /Pg 5582 0 R /P 40 0 R /K [1796 0 R 1797 0 R 1798 0 R 1799 0 R 1800 0 R 1801 0 R 1802 0 R 1803 0 R 1804 0 R 1805 0 R 1806 0 R] /S /P >> endobj 5451 0 obj << /Pg 5582 0 R /P 40 0 R /K 1807 0 R /S /P >> endobj 5452 0 obj << /Pg 5582 0 R /P 40 0 R /K [1808 0 R 1809 0 R 1810 0 R 1811 0 R 1812 0 R 1813 0 R 1814 0 R 1815 0 R 1816 0 R 1817 0 R] /S /P >> endobj 5453 0 obj << /Pg 5582 0 R /P 40 0 R /K [1818 0 R 1819 0 R 1820 0 R 1821 0 R 1822 0 R 1823 0 R 1824 0 R 1825 0 R 1826 0 R 1827 0 R 1828 0 R 1829 0 R 1830 0 R 1831 0 R 1832 0 R 1833 0 R 1834 0 R 1835 0 R 1836 0 R 1837 0 R 1838 0 R 1839 0 R 1840 0 R 1841 0 R 1842 0 R 1843 0 R] /S /P >> endobj 5454 0 obj << /Pg 5582 0 R /P 40 0 R /K [1844 0 R 1845 0 R 1846 0 R 1847 0 R 1848 0 R 1849 0 R 1850 0 R 1851 0 R 1852 0 R 1853 0 R 1854 0 R 1855 0 R 1856 0 R 1857 0 R 1858 0 R 1859 0 R 1860 0 R 1861 0 R 1862 0 R 1863 0 R 1864 0 R 1865 0 R 1866 0 R 1867 0 R 1868 0 R 1869 0 R 1870 0 R 1871 0 R 1872 0 R 1873 0 R 1874 0 R 1875 0 R 1876 0 R 1877 0 R 1878 0 R 1879 0 R 1880 0 R 1881 0 R 1882 0 R 1883 0 R 1884 0 R 1885 0 R 1886 0 R 1887 0 R 1888 0 R 1889 0 R 1890 0 R 1891 0 R 1892 0 R 1893 0 R 1894 0 R 1895 0 R 1896 0 R 1897 0 R 1898 0 R 1899 0 R 1900 0 R 1901 0 R 1902 0 R 1903 0 R 1904 0 R 1905 0 R 1906 0 R 1907 0 R 1908 0 R 1909 0 R 1910 0 R 1911 0 R 1912 0 R 1913 0 R 1914 0 R 1915 0 R 1916 0 R 1917 0 R 1918 0 R 1919 0 R 1920 0 R] /S /P >> endobj 5455 0 obj << /Pg 5583 0 R /P 40 0 R /K [1921 0 R 1922 0 R 1923 0 R 1924 0 R 1925 0 R 1926 0 R 1927 0 R 1928 0 R 1929 0 R 1930 0 R] /S /P >> endobj 5456 0 obj << /Pg 5583 0 R /P 40 0 R /K [1931 0 R 1932 0 R 1933 0 R 1934 0 R 1935 0 R 1936 0 R 1937 0 R 1938 0 R 1939 0 R 1940 0 R 1941 0 R 1942 0 R 1943 0 R 1944 0 R 1945 0 R 1946 0 R 1947 0 R 1948 0 R 1949 0 R 1950 0 R 1951 0 R 1952 0 R 1953 0 R 1954 0 R 1955 0 R 1956 0 R 1957 0 R 1958 0 R 1959 0 R 1960 0 R 1961 0 R 1962 0 R 1963 0 R 1964 0 R 1965 0 R 1966 0 R 1967 0 R 1968 0 R 1969 0 R 1970 0 R 1971 0 R 1972 0 R 1973 0 R 1974 0 R] /S /P >> endobj 5457 0 obj << /Pg 5583 0 R /P 40 0 R /K [1975 0 R 1976 0 R 1977 0 R 1978 0 R 1979 0 R 1980 0 R 1981 0 R 1982 0 R 1983 0 R 1984 0 R 1985 0 R 1986 0 R] /S /P >> endobj 5458 0 obj << /Pg 5583 0 R /P 40 0 R /K 1987 0 R /S /P >> endobj 5459 0 obj << /Pg 5583 0 R /P 40 0 R /K [1988 0 R 1989 0 R 1990 0 R 1991 0 R 1992 0 R] /S /P >> endobj 5460 0 obj << /Pg 5583 0 R /P 40 0 R /K [1993 0 R 1994 0 R 1995 0 R 1996 0 R 1997 0 R 1998 0 R 1999 0 R 2000 0 R 2001 0 R 2002 0 R 2003 0 R 2004 0 R 2005 0 R 2006 0 R 2007 0 R 2008 0 R 2009 0 R 2010 0 R 2011 0 R 2012 0 R 2013 0 R 2014 0 R 2015 0 R 2016 0 R 2017 0 R 2018 0 R 2019 0 R 2020 0 R 2021 0 R 2022 0 R 2023 0 R 2024 0 R 2025 0 R 2026 0 R 2027 0 R 2028 0 R 2029 0 R 2030 0 R 2031 0 R 2032 0 R 2033 0 R 2034 0 R 2035 0 R 2036 0 R 2037 0 R 2038 0 R 2039 0 R 2040 0 R 2041 0 R 2042 0 R 2043 0 R 2044 0 R 2045 0 R 2046 0 R 2047 0 R 2048 0 R 2049 0 R 2050 0 R 2051 0 R 2052 0 R] /S /P >> endobj 5461 0 obj << /Pg 5583 0 R /P 40 0 R /K [2053 0 R 2054 0 R 2055 0 R 2056 0 R 2057 0 R 2058 0 R 2059 0 R 2060 0 R 2061 0 R 2062 0 R 2063 0 R 2064 0 R 2065 0 R 2066 0 R 2067 0 R 2068 0 R 2069 0 R 2070 0 R 2071 0 R 2072 0 R 2073 0 R 2074 0 R 2075 0 R 2076 0 R 2077 0 R 2078 0 R 2079 0 R 2080 0 R 2081 0 R 2082 0 R 2083 0 R 2084 0 R 2085 0 R 2086 0 R 2087 0 R 2088 0 R 2089 0 R 2090 0 R 2091 0 R 2092 0 R 2093 0 R 2094 0 R 2095 0 R 2096 0 R 2097 0 R 2098 0 R 2099 0 R 2100 0 R] /S /P >> endobj 5462 0 obj << /Pg 5583 0 R /P 40 0 R /K [2101 0 R 2102 0 R 2103 0 R 2104 0 R 2105 0 R 2106 0 R 2107 0 R 2108 0 R 2109 0 R 2110 0 R] /S /P >> endobj 5463 0 obj << /Pg 5583 0 R /P 40 0 R /K 2111 0 R /S /P >> endobj 5464 0 obj << /Pg 5583 0 R /P 40 0 R /K [2112 0 R 2113 0 R 2114 0 R 2115 0 R 2116 0 R 2117 0 R 2118 0 R] /S /P >> endobj 5465 0 obj << /Pg 5584 0 R /P 40 0 R /K [2119 0 R 2120 0 R 2121 0 R 2122 0 R 2123 0 R 2124 0 R 2125 0 R 2126 0 R 2127 0 R 2128 0 R 2129 0 R 2130 0 R 2131 0 R 2132 0 R 2133 0 R 2134 0 R 2135 0 R 2136 0 R 2137 0 R 2138 0 R 2139 0 R 2140 0 R 2141 0 R 2142 0 R 2143 0 R 2144 0 R 2145 0 R 2146 0 R 2147 0 R 2148 0 R 2149 0 R 2150 0 R 2151 0 R 2152 0 R 2153 0 R 2154 0 R 2155 0 R 2156 0 R 2157 0 R 2158 0 R 2159 0 R 2160 0 R 2161 0 R 2162 0 R 2163 0 R 2164 0 R 2165 0 R 2166 0 R 2167 0 R 2168 0 R 2169 0 R 2170 0 R 2171 0 R 2172 0 R 2173 0 R 2174 0 R 2175 0 R 2176 0 R 2177 0 R 2178 0 R 2179 0 R 2180 0 R 2181 0 R 2182 0 R 2183 0 R 2184 0 R 2185 0 R 2186 0 R 2187 0 R 2188 0 R 2189 0 R 2190 0 R 2191 0 R 2192 0 R 2193 0 R 2194 0 R 2195 0 R 2196 0 R 2197 0 R 2198 0 R 2199 0 R 2200 0 R 2201 0 R 2202 0 R 2203 0 R 2204 0 R 2205 0 R 2206 0 R 2207 0 R 2208 0 R 2209 0 R 2210 0 R 2211 0 R 2212 0 R 2213 0 R 2214 0 R 2215 0 R 2216 0 R 2217 0 R 2218 0 R 2219 0 R 2220 0 R 2221 0 R 2222 0 R 2223 0 R 2224 0 R 2225 0 R 2226 0 R 2227 0 R 2228 0 R 2229 0 R 2230 0 R 2231 0 R 2232 0 R 2233 0 R 2234 0 R 2235 0 R 2236 0 R 2237 0 R 2238 0 R 2239 0 R 2240 0 R 2241 0 R 2242 0 R 2243 0 R] /S /P >> endobj 5466 0 obj << /Pg 5584 0 R /P 40 0 R /K 2244 0 R /S /P >> endobj 5467 0 obj << /Pg 5584 0 R /P 40 0 R /K [2245 0 R 2246 0 R 2247 0 R 2248 0 R] /S /P >> endobj 5468 0 obj << /Pg 5584 0 R /P 40 0 R /K [2249 0 R 2250 0 R 2251 0 R 2252 0 R 2253 0 R 2254 0 R 2255 0 R 2256 0 R 2257 0 R 2258 0 R 2259 0 R 2260 0 R 2261 0 R 2262 0 R 2263 0 R 2264 0 R 2265 0 R 2266 0 R 2267 0 R 2268 0 R 2269 0 R 2270 0 R 2271 0 R 2272 0 R 2273 0 R 2274 0 R 2275 0 R 2276 0 R 2277 0 R 2278 0 R 2279 0 R 2280 0 R 2281 0 R 2282 0 R 2283 0 R 2284 0 R 2285 0 R 2286 0 R 2287 0 R 2288 0 R 2289 0 R 2290 0 R 2291 0 R 2292 0 R 2293 0 R 2294 0 R 2295 0 R 2296 0 R 2297 0 R 2298 0 R 2299 0 R 2300 0 R 2301 0 R 2302 0 R 2303 0 R 2304 0 R 2305 0 R] /S /P >> endobj 5469 0 obj << /Pg 5584 0 R /P 40 0 R /K 2306 0 R /S /P >> endobj 5470 0 obj << /Pg 5584 0 R /P 40 0 R /K [2307 0 R 2308 0 R] /S /P >> endobj 5471 0 obj << /Pg 5585 0 R /P 40 0 R /K [2309 0 R 2310 0 R] /S /P >> endobj 5472 0 obj << /Pg 5585 0 R /P 40 0 R /K [2311 0 R 2312 0 R 2313 0 R 2314 0 R 2315 0 R 2316 0 R 2317 0 R 2318 0 R 2319 0 R 2320 0 R 2321 0 R 2322 0 R 2323 0 R 2324 0 R 2325 0 R 2326 0 R 2327 0 R 2328 0 R 2329 0 R 2330 0 R 2331 0 R 2332 0 R 2333 0 R 2334 0 R 2335 0 R 2336 0 R 2337 0 R 2338 0 R 2339 0 R 2340 0 R 2341 0 R 2342 0 R 2343 0 R 2344 0 R 2345 0 R 2346 0 R 2347 0 R 2348 0 R 2349 0 R 2350 0 R 2351 0 R 2352 0 R 2353 0 R 2354 0 R 2355 0 R 2356 0 R 2357 0 R] /S /P >> endobj 5473 0 obj << /Pg 5585 0 R /P 40 0 R /K [2358 0 R 2359 0 R 2360 0 R 2361 0 R 2362 0 R 2363 0 R 2364 0 R 2365 0 R 2366 0 R 2367 0 R 2368 0 R 2369 0 R 2370 0 R 2371 0 R 2372 0 R 2373 0 R 2374 0 R 2375 0 R 2376 0 R 2377 0 R 2378 0 R 2379 0 R 2380 0 R 2381 0 R 2382 0 R 2383 0 R 2384 0 R 2385 0 R 2386 0 R 2387 0 R 2388 0 R 2389 0 R 2390 0 R 2391 0 R 2392 0 R 2393 0 R 2394 0 R 2395 0 R 2396 0 R 2397 0 R 2398 0 R 2399 0 R 2400 0 R 2401 0 R 2402 0 R 2403 0 R 2404 0 R 2405 0 R 2406 0 R 2407 0 R 2408 0 R 2409 0 R 2410 0 R 2411 0 R 2412 0 R 2413 0 R 2414 0 R 2415 0 R 2416 0 R 2417 0 R 2418 0 R 2419 0 R 2420 0 R 2421 0 R 2422 0 R 2423 0 R 2424 0 R 2425 0 R 2426 0 R 2427 0 R 2428 0 R 2429 0 R 2430 0 R 2431 0 R 2432 0 R 2433 0 R 2434 0 R 2435 0 R 2436 0 R 2437 0 R 2438 0 R 2439 0 R 2440 0 R 2441 0 R 2442 0 R 2443 0 R 2444 0 R 2445 0 R 2446 0 R 2447 0 R 2448 0 R 2449 0 R 2450 0 R 2451 0 R 2452 0 R 2453 0 R 2454 0 R 2455 0 R 2456 0 R 2457 0 R 2458 0 R 2459 0 R 2460 0 R 2461 0 R 2462 0 R 2463 0 R 2464 0 R 2465 0 R 2466 0 R 2467 0 R 2468 0 R 2469 0 R 2470 0 R 2471 0 R 2472 0 R] /S /P >> endobj 5474 0 obj << /Pg 5585 0 R /P 40 0 R /K [2473 0 R 2474 0 R 2475 0 R 2476 0 R 2477 0 R 2478 0 R 2479 0 R 2480 0 R 2481 0 R 2482 0 R 2483 0 R 2484 0 R 2485 0 R 2486 0 R 2487 0 R 2488 0 R 2489 0 R 2490 0 R 2491 0 R 2492 0 R 2493 0 R 2494 0 R 2495 0 R 2496 0 R 2497 0 R 2498 0 R 2499 0 R 2500 0 R 2501 0 R 2502 0 R 2503 0 R 2504 0 R 2505 0 R 2506 0 R 2507 0 R 2508 0 R 2509 0 R 2510 0 R 2511 0 R 2512 0 R 2513 0 R 2514 0 R 2515 0 R 2516 0 R 2517 0 R 2518 0 R 2519 0 R 2520 0 R 2521 0 R 2522 0 R 2523 0 R 2524 0 R 2525 0 R 2526 0 R 2527 0 R 2528 0 R 2529 0 R 2530 0 R 2531 0 R 2532 0 R 2533 0 R 2534 0 R 2535 0 R 2536 0 R 2537 0 R 2538 0 R 2539 0 R 2540 0 R 2541 0 R 2542 0 R 2543 0 R 2544 0 R 2545 0 R 2546 0 R 2547 0 R 2548 0 R 2549 0 R 2550 0 R 2551 0 R 2552 0 R] /S /P >> endobj 5475 0 obj << /Pg 5586 0 R /P 40 0 R /K [2553 0 R 2554 0 R 2555 0 R 2556 0 R 2557 0 R 2558 0 R 2559 0 R 2560 0 R 2561 0 R 2562 0 R 2563 0 R 2564 0 R 2565 0 R 2566 0 R 2567 0 R 2568 0 R 2569 0 R 2570 0 R 2571 0 R 2572 0 R 2573 0 R 2574 0 R 2575 0 R 2576 0 R 2577 0 R 2578 0 R 2579 0 R 2580 0 R 2581 0 R 2582 0 R 2583 0 R 2584 0 R 2585 0 R 2586 0 R 2587 0 R 2588 0 R 2589 0 R 2590 0 R 2591 0 R 2592 0 R 2593 0 R 2594 0 R 2595 0 R 2596 0 R 2597 0 R 2598 0 R 2599 0 R 2600 0 R 2601 0 R 2602 0 R 2603 0 R 2604 0 R 2605 0 R 2606 0 R 2607 0 R] /S /P >> endobj 5476 0 obj << /Pg 5586 0 R /P 40 0 R /K [2608 0 R 2609 0 R 2610 0 R 2611 0 R 2612 0 R 2613 0 R 2614 0 R 2615 0 R 2616 0 R 2617 0 R 2618 0 R 2619 0 R 2620 0 R 2621 0 R 2622 0 R 2623 0 R 2624 0 R 2625 0 R 2626 0 R 2627 0 R 2628 0 R 2629 0 R 2630 0 R 2631 0 R 2632 0 R 2633 0 R 2634 0 R 2635 0 R 2636 0 R 2637 0 R 2638 0 R 2639 0 R 2640 0 R 2641 0 R 2642 0 R 2643 0 R 2644 0 R 2645 0 R 2646 0 R 2647 0 R 2648 0 R 2649 0 R 2650 0 R 2651 0 R 2652 0 R 2653 0 R 2654 0 R 2655 0 R 2656 0 R 2657 0 R 2658 0 R 2659 0 R 2660 0 R 2661 0 R 2662 0 R 2663 0 R 2664 0 R 2665 0 R 2666 0 R 2667 0 R 2668 0 R 2669 0 R 2670 0 R 2671 0 R 2672 0 R 2673 0 R 2674 0 R 2675 0 R 2676 0 R 2677 0 R 2678 0 R 2679 0 R 2680 0 R 2681 0 R 2682 0 R 2683 0 R 2684 0 R 2685 0 R 2686 0 R 2687 0 R] /S /P >> endobj 5477 0 obj << /Pg 5586 0 R /P 40 0 R /K [2688 0 R 2689 0 R 2690 0 R 2691 0 R 2692 0 R 2693 0 R 2694 0 R 2695 0 R 2696 0 R 2697 0 R 2698 0 R 2699 0 R 2700 0 R 2701 0 R 2702 0 R 2703 0 R 2704 0 R 2705 0 R 2706 0 R 2707 0 R 2708 0 R 2709 0 R 2710 0 R 2711 0 R 2712 0 R 2713 0 R 2714 0 R 2715 0 R 2716 0 R 2717 0 R 2718 0 R 2719 0 R 2720 0 R 2721 0 R 2722 0 R 2723 0 R 2724 0 R 2725 0 R 2726 0 R 2727 0 R 2728 0 R 2729 0 R 2730 0 R 2731 0 R 2732 0 R 2733 0 R 2734 0 R 2735 0 R 2736 0 R 2737 0 R 2738 0 R 2739 0 R 2740 0 R 2741 0 R 2742 0 R 2743 0 R 2744 0 R 2745 0 R 2746 0 R 2747 0 R 2748 0 R 2749 0 R 2750 0 R 2751 0 R 2752 0 R 2753 0 R 2754 0 R 2755 0 R 2756 0 R 2757 0 R 2758 0 R 2759 0 R 2760 0 R 2761 0 R] /S /P >> endobj 5478 0 obj << /Pg 5587 0 R /P 40 0 R /K [2762 0 R 2763 0 R 2764 0 R 2765 0 R 2766 0 R 2767 0 R 2768 0 R 2769 0 R 2770 0 R 2771 0 R 2772 0 R 2773 0 R 2774 0 R 2775 0 R 2776 0 R 2777 0 R 2778 0 R 2779 0 R 2780 0 R 2781 0 R 2782 0 R 2783 0 R 2784 0 R 2785 0 R 2786 0 R 2787 0 R 2788 0 R 2789 0 R 2790 0 R 2791 0 R 2792 0 R 2793 0 R 2794 0 R 2795 0 R 2796 0 R 2797 0 R 2798 0 R 2799 0 R 2800 0 R 2801 0 R 2802 0 R] /S /P >> endobj 5479 0 obj << /Pg 5587 0 R /P 40 0 R /K [2803 0 R 2804 0 R 2805 0 R 2806 0 R 2807 0 R 2808 0 R 2809 0 R 2810 0 R 2811 0 R 2812 0 R 2813 0 R 2814 0 R 2815 0 R 2816 0 R 2817 0 R 2818 0 R 2819 0 R 2820 0 R 2821 0 R 2822 0 R 2823 0 R 2824 0 R 2825 0 R 2826 0 R 2827 0 R 2828 0 R 2829 0 R 2830 0 R 2831 0 R] /S /P >> endobj 5480 0 obj << /Pg 5587 0 R /P 40 0 R /K [2832 0 R 2833 0 R 2834 0 R 2835 0 R 2836 0 R 2837 0 R 2838 0 R 2839 0 R 2840 0 R 2841 0 R 2842 0 R 2843 0 R 2844 0 R 2845 0 R 2846 0 R 2847 0 R 2848 0 R 2849 0 R 2850 0 R 2851 0 R 2852 0 R 2853 0 R 2854 0 R 2855 0 R 2856 0 R 2857 0 R 2858 0 R 2859 0 R 2860 0 R 2861 0 R 2862 0 R 2863 0 R 2864 0 R 2865 0 R 2866 0 R 2867 0 R 2868 0 R 2869 0 R 2870 0 R 2871 0 R 2872 0 R 2873 0 R 2874 0 R 2875 0 R 2876 0 R 2877 0 R 2878 0 R 2879 0 R 2880 0 R 2881 0 R 2882 0 R 2883 0 R 2884 0 R 2885 0 R 2886 0 R 2887 0 R 2888 0 R 2889 0 R 2890 0 R 2891 0 R 2892 0 R 2893 0 R 2894 0 R 2895 0 R 2896 0 R 2897 0 R 2898 0 R 2899 0 R 2900 0 R 2901 0 R 2902 0 R 2903 0 R 2904 0 R 2905 0 R 2906 0 R 2907 0 R 2908 0 R 2909 0 R 2910 0 R 2911 0 R 2912 0 R 2913 0 R] /S /P >> endobj 5481 0 obj << /Pg 5587 0 R /P 40 0 R /K [2914 0 R 2915 0 R 2916 0 R 2917 0 R 2918 0 R 2919 0 R 2920 0 R 2921 0 R 2922 0 R 2923 0 R 2924 0 R 2925 0 R 2926 0 R 2927 0 R 2928 0 R 2929 0 R 2930 0 R 2931 0 R] /S /P >> endobj 5482 0 obj << /Pg 5587 0 R /P 40 0 R /K [2932 0 R 2933 0 R] /S /P >> endobj 5483 0 obj << /Pg 5588 0 R /P 40 0 R /K [2934 0 R 2935 0 R] /S /P >> endobj 5484 0 obj << /Pg 5588 0 R /P 40 0 R /K [2936 0 R 2937 0 R 2938 0 R 2939 0 R 2940 0 R 2941 0 R 2942 0 R 2943 0 R 2944 0 R 2945 0 R 2946 0 R 2947 0 R 2948 0 R 2949 0 R 2950 0 R 2951 0 R 2952 0 R 2953 0 R 2954 0 R 2955 0 R 2956 0 R 2957 0 R 2958 0 R 2959 0 R 2960 0 R 2961 0 R 2962 0 R 2963 0 R 2964 0 R 2965 0 R 2966 0 R 2967 0 R 2968 0 R 2969 0 R 2970 0 R 2971 0 R 2972 0 R 2973 0 R 2974 0 R 2975 0 R 2976 0 R 2977 0 R 2978 0 R 2979 0 R 2980 0 R 2981 0 R 2982 0 R 2983 0 R 2984 0 R 2985 0 R 2986 0 R 2987 0 R 2988 0 R 2989 0 R 2990 0 R 2991 0 R 2992 0 R 2993 0 R 2994 0 R 2995 0 R 2996 0 R 2997 0 R 2998 0 R 2999 0 R 3000 0 R 3001 0 R 3002 0 R 3003 0 R 3004 0 R 3005 0 R 3006 0 R 3007 0 R 3008 0 R 3009 0 R 3010 0 R 3011 0 R 3012 0 R 3013 0 R 3014 0 R 3015 0 R 3016 0 R] /S /P >> endobj 5485 0 obj << /Pg 5588 0 R /P 40 0 R /K 3017 0 R /S /P >> endobj 5486 0 obj << /Pg 5588 0 R /P 40 0 R /K [3018 0 R 3019 0 R] /S /P >> endobj 5487 0 obj << /Pg 5588 0 R /P 40 0 R /K [3020 0 R 3021 0 R 3022 0 R 3023 0 R 3024 0 R 3025 0 R 3026 0 R 3027 0 R 3028 0 R 3029 0 R 3030 0 R 3031 0 R 3032 0 R 3033 0 R] /S /P >> endobj 5488 0 obj << /Pg 5588 0 R /P 40 0 R /K 3034 0 R /S /P >> endobj 5489 0 obj << /Pg 5588 0 R /P 40 0 R /K [3035 0 R 3036 0 R] /S /P >> endobj 5490 0 obj << /Pg 5588 0 R /P 40 0 R /K [3037 0 R 3038 0 R] /S /P >> endobj 5491 0 obj << /Pg 5588 0 R /P 40 0 R /K 3039 0 R /S /P >> endobj 5492 0 obj << /Pg 5588 0 R /P 40 0 R /K [3040 0 R 3041 0 R] /S /P >> endobj 5493 0 obj << /Pg 5036 0 R /P 40 0 R /K [3042 0 R 3043 0 R] /S /P >> endobj 5494 0 obj << /Pg 5036 0 R /P 40 0 R /K [3044 0 R 3045 0 R 3046 0 R 3047 0 R 3048 0 R 3049 0 R 3050 0 R 3051 0 R] /S /P >> endobj 5495 0 obj << /Pg 5036 0 R /P 40 0 R /K [3052 0 R 3053 0 R 3054 0 R 3055 0 R 3056 0 R 3057 0 R 3058 0 R 3059 0 R 3060 0 R] /S /P >> endobj 5496 0 obj << /Pg 5036 0 R /P 40 0 R /K [3061 0 R 3062 0 R 3063 0 R 3064 0 R 3065 0 R 3066 0 R 3067 0 R 3068 0 R 3069 0 R 3070 0 R 3071 0 R 3072 0 R 3073 0 R 3074 0 R] /S /P >> endobj 5497 0 obj << /Pg 5036 0 R /P 40 0 R /K [3075 0 R 3076 0 R 3077 0 R 3078 0 R 3079 0 R 3080 0 R 3081 0 R 3082 0 R 3083 0 R] /S /P >> endobj 5498 0 obj << /Pg 5036 0 R /P 40 0 R /K [3084 0 R 3085 0 R 3086 0 R 3087 0 R 3088 0 R 3089 0 R 3090 0 R 3091 0 R 3092 0 R 3093 0 R 3094 0 R 3095 0 R] /S /P >> endobj 5499 0 obj << /Pg 5036 0 R /P 40 0 R /K [3096 0 R 3097 0 R 3098 0 R 3099 0 R 3100 0 R 3101 0 R 3102 0 R 3103 0 R 3104 0 R 3105 0 R 3106 0 R 3107 0 R] /S /P >> endobj 5500 0 obj << /Pg 5036 0 R /P 40 0 R /K [3108 0 R 3109 0 R 3110 0 R 3111 0 R 3112 0 R 3113 0 R 3114 0 R 3115 0 R 3116 0 R 3117 0 R 3118 0 R 3119 0 R 3120 0 R 3121 0 R 3122 0 R 3123 0 R 3124 0 R 3125 0 R 3126 0 R 3127 0 R] /S /P >> endobj 5501 0 obj << /Pg 5036 0 R /P 40 0 R /K [3128 0 R 3129 0 R 3130 0 R 3131 0 R 3132 0 R 3133 0 R 3134 0 R 3135 0 R 3136 0 R 3137 0 R 3138 0 R 3139 0 R 3140 0 R 3141 0 R 3142 0 R] /S /P >> endobj 5502 0 obj << /Pg 5036 0 R /P 40 0 R /K [3143 0 R 3144 0 R 3145 0 R 3146 0 R 3147 0 R 3148 0 R 3149 0 R 3150 0 R 3151 0 R 3152 0 R] /S /P >> endobj 5503 0 obj << /Pg 5036 0 R /P 40 0 R /K [3153 0 R 3154 0 R 3155 0 R 3156 0 R 3157 0 R 3158 0 R 3159 0 R 3160 0 R 3161 0 R 3162 0 R 3163 0 R 3164 0 R 3165 0 R] /S /P >> endobj 5504 0 obj << /Pg 5036 0 R /P 40 0 R /K [3166 0 R 3167 0 R 3168 0 R 3169 0 R 3170 0 R 3171 0 R 3172 0 R 3173 0 R 3174 0 R 3175 0 R 3176 0 R 3177 0 R 3178 0 R 3179 0 R 3180 0 R 3181 0 R 3182 0 R 3183 0 R] /S /P >> endobj 5037 0 obj << /Pg 5036 0 R /P 40 0 R /K [3184 0 R 3185 0 R 3186 0 R 3187 0 R 3188 0 R 3189 0 R 4131 0 R 3201 0 R 3202 0 R] /S /P >> endobj 5505 0 obj << /Pg 5036 0 R /P 40 0 R /K [3203 0 R 3204 0 R 3205 0 R 3206 0 R 3207 0 R 3208 0 R 3209 0 R 3210 0 R 3211 0 R 3212 0 R 3213 0 R 3214 0 R 3215 0 R] /S /P >> endobj 5506 0 obj << /Pg 5036 0 R /P 40 0 R /K [3216 0 R 3217 0 R 3218 0 R 3219 0 R 3220 0 R 3221 0 R 3222 0 R 3223 0 R 3224 0 R 3225 0 R] /S /P >> endobj 5507 0 obj << /Pg 5036 0 R /P 40 0 R /K [3226 0 R 3227 0 R 3228 0 R 3229 0 R 3230 0 R 3231 0 R 3232 0 R 3233 0 R 3234 0 R 3235 0 R 3236 0 R 3237 0 R 3238 0 R] /S /P >> endobj 5508 0 obj << /Pg 5036 0 R /P 40 0 R /K [3239 0 R 3240 0 R 3241 0 R 3242 0 R 3243 0 R 3244 0 R 3245 0 R 3246 0 R 3247 0 R 3248 0 R 3249 0 R 3250 0 R 3251 0 R 3252 0 R 3253 0 R 3254 0 R 3255 0 R 3256 0 R 3257 0 R 3258 0 R] /S /P >> endobj 5509 0 obj << /Pg 5036 0 R /P 40 0 R /K [3259 0 R 3260 0 R 3261 0 R 3262 0 R] /S /P >> endobj 5510 0 obj << /Pg 5589 0 R /P 40 0 R /K [3263 0 R 3264 0 R 3265 0 R 3266 0 R 3267 0 R 3268 0 R 3269 0 R] /S /P >> endobj 5511 0 obj << /Pg 5589 0 R /P 40 0 R /K [3270 0 R 3271 0 R 3272 0 R 3273 0 R 3274 0 R 3275 0 R 3276 0 R 3277 0 R 3278 0 R 3279 0 R 3280 0 R 3281 0 R] /S /P >> endobj 5512 0 obj << /Pg 5589 0 R /P 40 0 R /K [3282 0 R 3283 0 R 3284 0 R 3285 0 R 3286 0 R 3287 0 R 3288 0 R 3289 0 R 3290 0 R 3291 0 R 3292 0 R] /S /P >> endobj 5513 0 obj << /Pg 5589 0 R /P 40 0 R /K [3293 0 R 3294 0 R 3295 0 R 3296 0 R 3297 0 R 3298 0 R 3299 0 R 3300 0 R 3301 0 R] /S /P >> endobj 5514 0 obj << /Pg 5589 0 R /P 40 0 R /K [3302 0 R 3303 0 R 3304 0 R 3305 0 R 3306 0 R 3307 0 R 3308 0 R 3309 0 R 3310 0 R 3311 0 R 3312 0 R 3313 0 R] /S /P >> endobj 5515 0 obj << /Pg 5589 0 R /P 40 0 R /K [3314 0 R 3315 0 R 3316 0 R 3317 0 R 3318 0 R 3319 0 R 3320 0 R 3321 0 R 3322 0 R 3323 0 R 3324 0 R 3325 0 R 3326 0 R 3327 0 R 3328 0 R 3329 0 R 3330 0 R 3331 0 R] /S /P >> endobj 5516 0 obj << /Pg 5589 0 R /P 40 0 R /K [3332 0 R 3333 0 R 3334 0 R 3335 0 R 3336 0 R 3337 0 R 3338 0 R 3339 0 R 3340 0 R 3341 0 R 3342 0 R 3343 0 R 3344 0 R] /S /P >> endobj 5517 0 obj << /Pg 5589 0 R /P 40 0 R /K [3345 0 R 3346 0 R 3347 0 R 3348 0 R 3349 0 R 3350 0 R 3351 0 R 3352 0 R 3353 0 R 3354 0 R 3355 0 R 3356 0 R 3357 0 R] /S /P >> endobj 5518 0 obj << /Pg 5589 0 R /P 40 0 R /K [3358 0 R 3359 0 R 3360 0 R 3361 0 R 3362 0 R 3363 0 R 3364 0 R 3365 0 R 3366 0 R 3367 0 R 3368 0 R 3369 0 R 3370 0 R 3371 0 R 3372 0 R 3373 0 R] /S /P >> endobj 5519 0 obj << /Pg 5589 0 R /P 40 0 R /K [3374 0 R 3375 0 R 3376 0 R 3377 0 R 3378 0 R 3379 0 R 3380 0 R 3381 0 R 3382 0 R 3383 0 R 3384 0 R 3385 0 R 3386 0 R 3387 0 R] /S /P >> endobj 5520 0 obj << /Pg 5589 0 R /P 40 0 R /K [3388 0 R 3389 0 R 3390 0 R 3391 0 R 3392 0 R 3393 0 R 3394 0 R 3395 0 R 3396 0 R 3397 0 R 3398 0 R 3399 0 R 3400 0 R 3401 0 R 3402 0 R 3403 0 R 3404 0 R 3405 0 R 3406 0 R] /S /P >> endobj 5521 0 obj << /Pg 5589 0 R /P 40 0 R /K [3407 0 R 3408 0 R 3409 0 R 3410 0 R 3411 0 R 3412 0 R 3413 0 R 3414 0 R 3415 0 R 3416 0 R 3417 0 R 3418 0 R 3419 0 R 3420 0 R 3421 0 R 3422 0 R] /S /P >> endobj 5522 0 obj << /Pg 5589 0 R /P 40 0 R /K [3423 0 R 3424 0 R 3425 0 R 3426 0 R 3427 0 R 3428 0 R 3429 0 R 3430 0 R 3431 0 R 3432 0 R 3433 0 R 3434 0 R 3435 0 R 3436 0 R 3437 0 R 3438 0 R 3439 0 R 3440 0 R 3441 0 R] /S /P >> endobj 5523 0 obj << /Pg 5589 0 R /P 40 0 R /K [3442 0 R 3443 0 R 3444 0 R 3445 0 R 3446 0 R 3447 0 R 3448 0 R 3449 0 R 3450 0 R 3451 0 R 3452 0 R 3453 0 R 3454 0 R 3455 0 R 3456 0 R 3457 0 R 3458 0 R 3459 0 R 3460 0 R] /S /P >> endobj 5524 0 obj << /Pg 5589 0 R /P 40 0 R /K [3461 0 R 3462 0 R 3463 0 R 3464 0 R 3465 0 R 3466 0 R 3467 0 R 3468 0 R 3469 0 R 3470 0 R 3471 0 R 3472 0 R 3473 0 R 3474 0 R] /S /P >> endobj 5525 0 obj << /Pg 5589 0 R /P 40 0 R /K [3475 0 R 3476 0 R 3477 0 R 3478 0 R 3479 0 R 3480 0 R 3481 0 R 3482 0 R 3483 0 R 3484 0 R 3485 0 R 3486 0 R] /S /P >> endobj 5526 0 obj << /Pg 5590 0 R /P 40 0 R /K [3487 0 R 3488 0 R 3489 0 R 3490 0 R] /S /P >> endobj 5527 0 obj << /Pg 5590 0 R /P 40 0 R /K [3491 0 R 3492 0 R 3493 0 R 3494 0 R 3495 0 R 3496 0 R 3497 0 R 3498 0 R 3499 0 R 3500 0 R 3501 0 R 3502 0 R 3503 0 R] /S /P >> endobj 5528 0 obj << /Pg 5590 0 R /P 40 0 R /K [3504 0 R 3505 0 R 3506 0 R 3507 0 R 3508 0 R 3509 0 R 3510 0 R 3511 0 R 3512 0 R 3513 0 R 3514 0 R 3515 0 R 3516 0 R 3517 0 R 3518 0 R 3519 0 R] /S /P >> endobj 5529 0 obj << /Pg 5590 0 R /P 40 0 R /K [3520 0 R 3521 0 R 3522 0 R 3523 0 R 3524 0 R 3525 0 R 3526 0 R 3527 0 R 3528 0 R 3529 0 R 3530 0 R 3531 0 R] /S /P >> endobj 5530 0 obj << /Pg 5590 0 R /P 40 0 R /K [3532 0 R 3533 0 R 3534 0 R 3535 0 R 3536 0 R 3537 0 R 3538 0 R 3539 0 R 3540 0 R 3541 0 R 3542 0 R 3543 0 R 3544 0 R 3545 0 R 3546 0 R 3547 0 R 3548 0 R 3549 0 R] /S /P >> endobj 5531 0 obj << /Pg 5590 0 R /P 40 0 R /K 3550 0 R /S /P >> endobj 5532 0 obj << /Pg 5590 0 R /P 40 0 R /K 3551 0 R /S /P >> endobj 5533 0 obj << /Pg 5590 0 R /P 40 0 R /K 3552 0 R /S /P >> endobj 5534 0 obj << /Pg 5590 0 R /P 40 0 R /K 3553 0 R /S /P >> endobj 5535 0 obj << /Pg 5590 0 R /P 40 0 R /K 3554 0 R /S /P >> endobj 5536 0 obj << /Pg 5590 0 R /P 40 0 R /K 3555 0 R /S /P >> endobj 5537 0 obj << /Pg 5590 0 R /P 40 0 R /K 3556 0 R /S /P >> endobj 5538 0 obj << /Pg 5590 0 R /P 40 0 R /K 3557 0 R /S /P >> endobj 5539 0 obj << /Pg 5590 0 R /P 40 0 R /K 3558 0 R /S /P >> endobj 5540 0 obj << /Pg 5590 0 R /P 40 0 R /K [3559 0 R 3560 0 R] /S /P >> endobj 5541 0 obj << /Pg 5591 0 R /P 40 0 R /K [3561 0 R 3562 0 R] /S /P >> endobj 5542 0 obj << /Pg 5591 0 R /P 40 0 R /K 3563 0 R /S /P >> endobj 5543 0 obj << /Pg 5591 0 R /P 40 0 R /K [3564 0 R 3565 0 R 3566 0 R 3567 0 R 3568 0 R 3569 0 R 3570 0 R 3571 0 R 3572 0 R 3573 0 R 3574 0 R 3575 0 R 3576 0 R 3577 0 R 3578 0 R 3579 0 R 3580 0 R 3581 0 R 3582 0 R 3583 0 R 3584 0 R 3585 0 R 3586 0 R 3587 0 R 3588 0 R 3589 0 R 3590 0 R 3591 0 R 3592 0 R 3593 0 R 3594 0 R 3595 0 R 3596 0 R 3597 0 R 3598 0 R 3599 0 R 3600 0 R 3601 0 R 3602 0 R 3603 0 R 3604 0 R 3605 0 R 3606 0 R 3607 0 R 3608 0 R 3609 0 R 3610 0 R 3611 0 R 3612 0 R 3613 0 R 3614 0 R 3615 0 R 3616 0 R 3617 0 R 3618 0 R 3619 0 R 3620 0 R 3621 0 R 3622 0 R 3623 0 R 3624 0 R 3625 0 R 3626 0 R 3627 0 R 3628 0 R 3629 0 R 3630 0 R 3631 0 R 3632 0 R 3633 0 R 3634 0 R] /S /P >> endobj 5544 0 obj << /Pg 5591 0 R /P 40 0 R /K 3635 0 R /S /P >> endobj 5545 0 obj << /Pg 5591 0 R /P 40 0 R /K [3636 0 R 3637 0 R 3638 0 R 3639 0 R 3640 0 R 3641 0 R 3642 0 R 3643 0 R 3644 0 R 3645 0 R 3646 0 R 3647 0 R 3648 0 R 3649 0 R 3650 0 R 3651 0 R 3652 0 R 3653 0 R 3654 0 R 3655 0 R 3656 0 R 3657 0 R 3658 0 R 3659 0 R 3660 0 R 3661 0 R 3662 0 R 3663 0 R 3664 0 R 3665 0 R 3666 0 R 3667 0 R 3668 0 R 3669 0 R 3670 0 R 3671 0 R 3672 0 R 3673 0 R 3674 0 R 3675 0 R 3676 0 R 3677 0 R 3678 0 R 3679 0 R 3680 0 R 3681 0 R 3682 0 R 3683 0 R 3684 0 R 3685 0 R 3686 0 R 3687 0 R 3688 0 R 3689 0 R 3690 0 R 3691 0 R 3692 0 R 3693 0 R 3694 0 R 3695 0 R 3696 0 R 3697 0 R 3698 0 R 3699 0 R 3700 0 R 3701 0 R 3702 0 R 3703 0 R 3704 0 R 3705 0 R 3706 0 R 3707 0 R 3708 0 R 3709 0 R 3710 0 R 3711 0 R 3712 0 R 3713 0 R 3714 0 R 3715 0 R 3716 0 R 3717 0 R 3718 0 R 3719 0 R] /S /P >> endobj 5546 0 obj << /Pg 5592 0 R /P 40 0 R /K [3720 0 R 3721 0 R 3722 0 R 3723 0 R 3724 0 R 3725 0 R 3726 0 R 3727 0 R 3728 0 R 3729 0 R 3730 0 R 3731 0 R 3732 0 R 3733 0 R 3734 0 R 3735 0 R 3736 0 R 3737 0 R 3738 0 R 3739 0 R 3740 0 R 3741 0 R 3742 0 R] /S /P >> endobj 5547 0 obj << /Pg 5592 0 R /P 40 0 R /K 3743 0 R /S /P >> endobj 5548 0 obj << /Pg 5592 0 R /P 40 0 R /K [3744 0 R 3745 0 R 3746 0 R 3747 0 R] /S /P >> endobj 5549 0 obj << /Pg 5592 0 R /P 40 0 R /K [3748 0 R 3749 0 R 3750 0 R 3751 0 R 3752 0 R 3753 0 R 3754 0 R 3755 0 R 3756 0 R 3757 0 R 3758 0 R 3759 0 R 3760 0 R 3761 0 R 3762 0 R 3763 0 R 3764 0 R 3765 0 R 3766 0 R 3767 0 R 3768 0 R 3769 0 R 3770 0 R 3771 0 R 3772 0 R 3773 0 R 3774 0 R 3775 0 R 3776 0 R 3777 0 R 3778 0 R 3779 0 R 3780 0 R 3781 0 R 3782 0 R 3783 0 R 3784 0 R 3785 0 R 3786 0 R 3787 0 R 3788 0 R 3789 0 R 3790 0 R 3791 0 R 3792 0 R 3793 0 R 3794 0 R 3795 0 R 3796 0 R 3797 0 R 3798 0 R 3799 0 R 3800 0 R 3801 0 R 3802 0 R 3803 0 R 3804 0 R 3805 0 R 3806 0 R] /S /P >> endobj 5550 0 obj << /Pg 5592 0 R /P 40 0 R /K 3807 0 R /S /P >> endobj 5551 0 obj << /Pg 5592 0 R /P 40 0 R /K [3808 0 R 3809 0 R 3810 0 R 3811 0 R 3812 0 R 3813 0 R 3814 0 R 3815 0 R 3816 0 R 3817 0 R 3818 0 R 3819 0 R 3820 0 R 3821 0 R 3822 0 R 3823 0 R 3824 0 R 3825 0 R 3826 0 R 3827 0 R 3828 0 R 3829 0 R 3830 0 R 3831 0 R 3832 0 R 3833 0 R 3834 0 R 3835 0 R 3836 0 R 3837 0 R 3838 0 R 3839 0 R 3840 0 R 3841 0 R 3842 0 R 3843 0 R 3844 0 R 3845 0 R 3846 0 R 3847 0 R 3848 0 R 3849 0 R 3850 0 R 3851 0 R 3852 0 R 3853 0 R 3854 0 R 3855 0 R 3856 0 R 3857 0 R 3858 0 R 3859 0 R 3860 0 R 3861 0 R 3862 0 R 3863 0 R 3864 0 R 3865 0 R 3866 0 R 3867 0 R 3868 0 R 3869 0 R 3870 0 R 3871 0 R 3872 0 R 3873 0 R 3874 0 R 3875 0 R 3876 0 R 3877 0 R 3878 0 R 3879 0 R 3880 0 R 3881 0 R 3882 0 R 3883 0 R 3884 0 R 3885 0 R 3886 0 R 3887 0 R 3888 0 R 3889 0 R 3890 0 R 3891 0 R 3892 0 R 3893 0 R 3894 0 R 3895 0 R 3896 0 R 3897 0 R 3898 0 R 3899 0 R 3900 0 R 3901 0 R] /S /P >> endobj 5552 0 obj << /Pg 5593 0 R /P 40 0 R /K [3902 0 R 3903 0 R 3904 0 R 3905 0 R 3906 0 R 3907 0 R 3908 0 R 3909 0 R 3910 0 R 3911 0 R 3912 0 R 3913 0 R 3914 0 R 3915 0 R 3916 0 R 3917 0 R] /S /P >> endobj 5553 0 obj << /Pg 5593 0 R /P 40 0 R /K 3918 0 R /S /P >> endobj 5554 0 obj << /Pg 5593 0 R /P 40 0 R /K [3919 0 R 3920 0 R 3921 0 R 3922 0 R 3923 0 R 3924 0 R 3925 0 R 3926 0 R 3927 0 R 3928 0 R 3929 0 R 3930 0 R 3931 0 R 3932 0 R 3933 0 R 3934 0 R 3935 0 R 3936 0 R 3937 0 R 3938 0 R 3939 0 R 3940 0 R 3941 0 R 3942 0 R 3943 0 R 3944 0 R 3945 0 R 3946 0 R 3947 0 R 3948 0 R 3949 0 R 3950 0 R 3951 0 R 3952 0 R 3953 0 R 3954 0 R 3955 0 R 3956 0 R 3957 0 R 3958 0 R 3959 0 R 3960 0 R 3961 0 R 3962 0 R 3963 0 R 3964 0 R 3965 0 R 3966 0 R 3967 0 R 3968 0 R 3969 0 R 3970 0 R 3971 0 R 3972 0 R 3973 0 R 3974 0 R 3975 0 R 3976 0 R 3977 0 R 3978 0 R 3979 0 R 3980 0 R 3981 0 R 3982 0 R 3983 0 R 3984 0 R 3985 0 R 3986 0 R 3987 0 R 3988 0 R 3989 0 R 3990 0 R 3991 0 R 3992 0 R 3993 0 R 3994 0 R 3995 0 R 3996 0 R 3997 0 R 3998 0 R 3999 0 R 4000 0 R 4001 0 R 4002 0 R 4003 0 R 4004 0 R 4005 0 R 4006 0 R 4007 0 R 4008 0 R 4009 0 R] /S /P >> endobj 5555 0 obj << /Pg 5593 0 R /P 40 0 R /K 4010 0 R /S /P >> endobj 5556 0 obj << /Pg 5593 0 R /P 40 0 R /K [4011 0 R 4012 0 R 4013 0 R 4014 0 R 4015 0 R 4016 0 R 4017 0 R 4018 0 R 4019 0 R 4020 0 R 4021 0 R 4022 0 R 4023 0 R 4024 0 R 4025 0 R 4026 0 R 4027 0 R 4028 0 R 4029 0 R 4030 0 R 4031 0 R 4032 0 R 4033 0 R 4034 0 R 4035 0 R 4036 0 R 4037 0 R 4038 0 R 4039 0 R 4040 0 R 4041 0 R 4042 0 R 4043 0 R 4044 0 R 4045 0 R 4046 0 R 4047 0 R 4048 0 R 4049 0 R 4050 0 R 4051 0 R 4052 0 R 4053 0 R 4054 0 R 4055 0 R 4056 0 R 4057 0 R 4058 0 R 4059 0 R 4060 0 R 4061 0 R 4062 0 R 4063 0 R 4064 0 R 4065 0 R 4066 0 R 4067 0 R 4068 0 R 4069 0 R 4070 0 R 4071 0 R 4072 0 R 4073 0 R 4074 0 R 4075 0 R 4076 0 R] /S /P >> endobj 5557 0 obj << /Pg 5594 0 R /P 40 0 R /K [4077 0 R 4078 0 R 4079 0 R 4080 0 R 4081 0 R 4082 0 R 4083 0 R 4084 0 R 4085 0 R 4086 0 R 4087 0 R 4088 0 R] /S /P >> endobj 5558 0 obj << /Pg 5594 0 R /P 40 0 R /K 4089 0 R /S /P >> endobj 5559 0 obj << /Pg 5594 0 R /P 40 0 R /K [4090 0 R 4091 0 R 4092 0 R 4093 0 R 4094 0 R 4095 0 R 4096 0 R 4097 0 R 4098 0 R 4099 0 R 4100 0 R 4101 0 R 4102 0 R 4103 0 R 4104 0 R 4105 0 R 4106 0 R 4107 0 R 4108 0 R 4109 0 R 4110 0 R 4111 0 R 4112 0 R 4113 0 R 4114 0 R 4115 0 R 4116 0 R 4117 0 R 4118 0 R 4119 0 R 4120 0 R 4121 0 R 4122 0 R 4123 0 R 4124 0 R 4125 0 R 4126 0 R 4127 0 R 4128 0 R 4129 0 R] /S /P >> endobj 5560 0 obj << /Pg 5594 0 R /P 40 0 R /K 4130 0 R /S /P >> endobj 4130 0 obj << /Pg 5594 0 R /P 5560 0 R /K 53 /ActualText ( ) /S /Span >> endobj 5594 0 obj << /Group << /CS /DeviceRGB /Type /Group /S /Transparency >> /StructParents 22 /Parent 9 0 R /Resources << /Font << /F2 5029 0 R /F1 5026 0 R /F3 5031 0 R >> /ProcSet [/PDF /Text /ImageB /ImageC /ImageI] >> /MediaBox [0 0 595.44 842.04] /Tabs /S /Type /Page /Contents 5595 0 R >> endobj 5595 0 obj << /Filter /FlateDecode /Length 2539 >> stream xZYo6~7V!J8 $xe1wK*J-bTux^~8jַ߯f}^.ӓbLT3O{j5VWTfXsbnqcm[Upݻ?lÓ7܍_բ];F~6s-#G90 ܘ6yrIUo8'_5ZhDj#DNVxAևH0 @`+Uo?T'G{Wj`AմlԂ H7L)ŋ+ X9Z-C`?g  R+L\8,΁h|EY#΋5:0spF['=xmrsǼؠRr"Ş3CX pپoQP5Հ]j-ir,ט&9FYUQ@KõZ#?9gqio!nqA~Dr~t3FԻ:rӱ.X1Ѡ5PċIH9gjT#wc+z`Hw|I 6'nWĶ>_H;[:WSY }x? f0bc`H@}Wg*uټX=R Hzy~+-pɃ=q&o W!kYgZBr؃Q+=b#fuzQ] kg{`**X: ǧ4(V. *Ϳ,quF&筕rzcF]lVl^ɳ{'fCq.l7o{Ҁ)!ҵnMɤRol(ԏc;F`}C1a!M&NJE8Bk)Ǭu$$F]R>c(Ǚԏ. AzY wsh<؁I>vzrbu,\OMewLTjn}c_6𡤳W>AW1 oX^]VI&K8?[y[uw^oꮋpy_]`+Ǝ >ÆFDN%]y^_ C1մ2֩]\19 Kf{;aRyaҒi[GA6`aJxL #xrZCSFź,CIKDO^!侨eCjI2)Fo(c{n87sDēxpa:I3y63P<$ PBޘt',rq'VΞ?4Mʩ|ɅݞYSK|cy]oc}L.Hс@$.oxp?li?4WQ:nNM^Jͺ!b EQ6* Od:̥(&p%ͻCżr8]OcT'YN}1iO*Ymg m;BN}dXa]Gm#lr2DA;2ڶ+\h n"P"$`Jj\V\EEǢz08I2@yJ0{g|imPHڱumtӦ&Лh8[ F/Y(HCSpm$I97 endstream endobj 9 0 obj << /Parent 2 0 R /Kids [5590 0 R 5591 0 R 5592 0 R 5593 0 R 5594 0 R] /Type /Pages /Count 5 >> endobj 5590 0 obj << /Group << /CS /DeviceRGB /Type /Group /S /Transparency >> /StructParents 18 /Parent 9 0 R /Resources << /ExtGState << /GS37 5355 0 R >> /Font << /F2 5029 0 R /F8 5596 0 R /F7 5597 0 R >> /ProcSet [/PDF /Text /ImageB /ImageC /ImageI] >> /MediaBox [0 0 595.44 842.04] /Tabs /S /Type /Page /Contents 5598 0 R >> endobj 5591 0 obj << /Group << /CS /DeviceRGB /Type /Group /S /Transparency >> /StructParents 19 /Parent 9 0 R /Resources << /Font << /F2 5029 0 R /F1 5026 0 R /F6 5352 0 R /F4 5034 0 R >> /ProcSet [/PDF /Text /ImageB /ImageC /ImageI] >> /MediaBox [0 0 595.44 842.04] /Tabs /S /Type /Page /Contents 5599 0 R >> endobj 5592 0 obj << /Group << /CS /DeviceRGB /Type /Group /S /Transparency >> /StructParents 20 /Parent 9 0 R /Resources << /Font << /F2 5029 0 R /F1 5026 0 R /F6 5352 0 R /F4 5034 0 R >> /ProcSet [/PDF /Text /ImageB /ImageC /ImageI] >> /MediaBox [0 0 595.44 842.04] /Tabs /S /Type /Page /Contents 5600 0 R >> endobj 5593 0 obj << /Group << /CS /DeviceRGB /Type /Group /S /Transparency >> /StructParents 21 /Parent 9 0 R /Resources << /ExtGState << /GS37 5355 0 R /GS36 5356 0 R >> /Font << /F2 5029 0 R /F1 5026 0 R /F6 5352 0 R /F4 5034 0 R /F3 5031 0 R >> /ProcSet [/PDF /Text /ImageB /ImageC /ImageI] >> /MediaBox [0 0 595.44 842.04] /Tabs /S /Type /Page /Contents 5601 0 R >> endobj 5601 0 obj << /Filter /FlateDecode /Length 5068 >> stream x=َdTTHflcHfjHcj Lf0SZ,ZU 8|t?xpzN{u;a^OqН/=AÛw7>{o^<9۫Q€  Υ 1'QJ(xt'RP3;0F z  \a0' s r$|#|1[wZ3`<`?'=4s >NL0[؎"N3OsHw⦚ZS 1wLbIenB ѡN`ߝ<$? 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|,zcM `SF|#,o7fWGFHRvԛ 0݁K aW!Vr(22XNè/7r#zf&H%D"Ewa@t(zED`Q,LJg @bofp/ss8@ )@ 8@ A w#&Db"1@L &Db"1@L &Db"1@L &Db"1@L &Db"1@L &Db"1@L &Db"1@L &Db"1@L &Db"1@L &Db"1Eh+C 3DF9uaH 7-I]rte@p TAN&lQتs`6N4XRuɄ$k.[LS*?ܺda@֓5"pk o~%}p=)nWφ=Q+:{߮ɛqzeXݯ|OQuyTA!no2{V=/|.mq,~ODUd-}=뮬r"(yR-'qWIK~h*P^M zC=I)n;>Ћ Rkp+J1>!gfFx|&AgJ-e?OKoFDOZ>b{mMϱv;hҺE@#'Ԣ-Oh>Q~/en]{DHdž5d>̂/ffb.SPifn8iť^^K_ջ'ݲwrܣ'V-Vwٳss?Qh~f sA=W` >.y'3.{@LyGKelQ>;r:{5b=ZRtg6J~[eꍒa,t7̓H qWg-cb q֥ڲ&nN&} OdB;τSU:WoL<9igk`bgb`76707707&A?@n˂2ffj"wdzCEC^ chЀ)2̀4G.w BB2 |q$q.M~4zʷ55np󹺫|:~gf{39F+HL?Tv%>ER=: 6#+YS'|geYBZ]jk4vdi+< Rk80ZmlFfEyגFؕS挝_%~jcz3x3-rvnOщ~sEY?rVD>٣ܡ]< Aئ#U]h:;RR!Orױ eS4le8,KHkxiOWm HTS$7H9z} Tyo ?p6|Mj/Jܑf)tXEU{l;BI^g$&]R}iqhl@YUE߼> ^{ksg'?fQlse^jVd_w,5i9d:#8 c_aP,v~0׃ 1A_g)KdVƆ.0\7a7cf775Mh<XϏ K;iF<1I1U>UIߣʿo)j~Ģp<ֱ7ޓ:ŝv-trSorsYnl℠aںA;s]fLqi "-K;$E72L塀n_{sKR5/la žEѪO݅y5'ρFW^{q$ !rߊbBBHqLL)kɔUɒϸڹ5FgydOnĽJNNkH # qȶ:oc)Ws 8!`pbg|xY#<߿7Π0nH|5 Hl]wh~E'i܃i^ AڞڄՃ5,z*Rw\?z*jrÓ.2u#4b>|zo(- 戬˦C NL&^ͲTz ,x7qR/"r`]yK%'Ll;b+ȖڄY3 Kd1yU=,6dE챘tCݲI~0N[*E* :٧k/V?ؾ;L.=}G+ $'&o"H̑kެj\y;Wn'ipbw7Kюc {@hA`UeFQGwO蔈mrJAuKz@eOkC=Y% eE9n_ΝfɌ*Rh-^*.xxFg76?zQ{;@9놳|^VSOs}&I ~RW'WaÜOZ HTTbse {oD;:襺F~Dn1/i(1 ?)qiޘϨl $y63DQq9 =*n+O3G9C`Νt#]_r82e u*B)dJ['ȔTp m¬hye1 C"qN&J|ElcXŠIPK5Wh9Q_ 0TQU ܕVw3Q`Bvΰmr_Jm6{12e Tʹ$wvҵ3ݦ_tIIL >/r@1>-rX-~D ŽV#½EBf}ƬܢK*.=1금S3xudͻS_G הx-j0OQ˛h[E'Z$dEYi})Qb"Ë=^ ~x1WNYh};:? 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117 /ActualText (BALF.) /S /Span >> endobj 3838 0 obj << /Pg 5592 0 R /P 5551 0 R /K 118 /ActualText ( ) /S /Span >> endobj 3839 0 obj << /Pg 5592 0 R /P 5551 0 R /K 119 /ActualText (\(c\)) /S /Span >> endobj 3840 0 obj << /Pg 5592 0 R /P 5551 0 R /K 120 /ActualText ( ) /S /Span >> endobj 3841 0 obj << /Pg 5592 0 R /P 5551 0 R /K 121 /ActualText (Time course of the amounts of neutrophils or macrophages in BALF intratracheally ) /S /Span >> endobj 3842 0 obj << /Pg 5592 0 R /P 5551 0 R /K 122 /ActualText (treated with LPS \(1 ) /S /Span >> endobj 3843 0 obj << /Pg 5592 0 R /P 5551 0 R /K 123 /ActualText (m) /S /Span >> endobj 3844 0 obj << /Pg 5592 0 R /P 5551 0 R /K 124 /ActualText (g for twice\) or exposed to CS for 2 weeks.) /S /Span >> endobj 3845 0 obj << /Pg 5592 0 R /P 5551 0 R /K 125 /ActualText ( ) /S /Span >> endobj 3846 0 obj << /Pg 5592 0 R /P 5551 0 R /K 126 /ActualText (\() /S /Span >> endobj 3847 0 obj << /Pg 5592 0 R /P 5551 0 R /K 127 /ActualText (d) /S /Span >> endobj 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172 /ActualText ( ) /S /Span >> endobj 3893 0 obj << /Pg 5592 0 R /P 5551 0 R /K 173 /ActualText (\() /S /Span >> endobj 3894 0 obj << /Pg 5592 0 R /P 5551 0 R /K 174 /ActualText (h) /S /Span >> endobj 3895 0 obj << /Pg 5592 0 R /P 5551 0 R /K 175 /ActualText (\) ) /S /Span >> endobj 3896 0 obj << /Pg 5592 0 R /P 5551 0 R /K 176 /ActualText (were detected. Mac: macrophages; ) /S /Span >> endobj 3897 0 obj << /Pg 5592 0 R /P 5551 0 R /K 177 /ActualText (Neu: neutrophils; Lym: lymphocytes; Eos: eosinophils. Th) /S /Span >> endobj 3898 0 obj << /Pg 5592 0 R /P 5551 0 R /K 178 /ActualText (roughout, data are representative of ) /S /Span >> endobj 3899 0 obj << /Pg 5592 0 R /P 5551 0 R /K 179 /ActualText (5) /S /Span >> endobj 3900 0 obj << /Pg 5592 0 R /P 5551 0 R /K 180 /ActualText (-) /S /Span >> endobj 3901 0 obj << /Pg 5592 0 R /P 5551 0 R /K 181 /ActualText (6 mice and were replicated in at least 3 independent experiments. Data are mean s.e.m. *P ) /S /Span >> endobj 3807 0 obj << /Pg 5592 0 R /P 5550 0 R /K 87 /ActualText ( ) /S /Span >> endobj 3748 0 obj << /Pg 5592 0 R /P 5549 0 R /K 28 /ActualText (Il17a) /S /Span >> endobj 3749 0 obj << /Pg 5592 0 R /P 5549 0 R /K 29 /ActualText (-) /S /Span >> endobj 3750 0 obj << /Pg 5592 0 R /P 5549 0 R /K 30 /ActualText (/) /S /Span >> endobj 3751 0 obj << /Pg 5592 0 R /P 5549 0 R /K 31 /ActualText (-) /S /Span >> endobj 3752 0 obj << /Pg 5592 0 R /P 5549 0 R /K 32 /ActualText ( ) /S /Span >> endobj 3753 0 obj << /Pg 5592 0 R /P 5549 0 R /K 33 /ActualText (mice and wildtype controls \(WT\) were sensitized with CS and challenged with elastin, ) /S /Span >> endobj 3754 0 obj << /Pg 5592 0 R /P 5549 0 R /K 34 /ActualText (and the inflammatory cell counts in BALF ) /S /Span >> endobj 3755 0 obj << /Pg 5592 0 R /P 5549 0 R /K 35 /ActualText (\() /S /Span >> endobj 3756 0 obj << /Pg 5592 0 R /P 5549 0 R /K 36 /ActualText (a) /S /Span >> endobj 3757 0 obj << /Pg 5592 0 R /P 5549 0 R /K 37 /ActualText (\)) /S /Span >> endobj 3758 0 obj << /Pg 5592 0 R /P 5549 0 R /K 38 /ActualText (, H&E staining and the semi) /S /Span >> endobj 3759 0 obj << /Pg 5592 0 R /P 5549 0 R /K 39 /ActualText (-) /S /Span >> endobj 3760 0 obj << /Pg 5592 0 R /P 5549 0 R /K 40 /ActualText (quantified ) /S /Span >> endobj 3761 0 obj << /Pg 5592 0 R /P 5549 0 R /K 41 /ActualText (inflammatory scoring ) /S /Span >> endobj 3762 0 obj << /Pg 5592 0 R /P 5549 0 R /K 42 /ActualText (\() /S /Span >> endobj 3763 0 obj << /Pg 5592 0 R /P 5549 0 R /K 43 /ActualText (b) /S /Span >> endobj 3764 0 obj << /Pg 5592 0 R /P 5549 0 R /K 44 /ActualText (\)) /S /Span >> endobj 3765 0 obj << /Pg 5592 0 R /P 5549 0 R /K 45 /ActualText (,) /S /Span >> endobj 3766 0 obj << /Pg 5592 0 R /P 5549 0 R /K 46 /ActualText ( ) /S /Span >> endobj 3767 0 obj << /Pg 5592 0 R /P 5549 0 R /K 47 /ActualText (t) /S /Span >> endobj 3768 0 obj << /Pg 5592 0 R /P 5549 0 R /K 48 /ActualText (he concentrations of CXCL1, CXCL2, and IL6 in BALF) /S /Span >> endobj 3769 0 obj << /Pg 5592 0 R /P 5549 0 R /K 49 /ActualText ( ) /S /Span >> endobj 3770 0 obj << /Pg 5592 0 R /P 5549 0 R /K 50 /ActualText (\() /S /Span >> endobj 3771 0 obj << /Pg 5592 0 R /P 5549 0 R /K 51 /ActualText (c) /S /Span >> endobj 3772 0 obj << /Pg 5592 0 R /P 5549 0 R /K 52 /ActualText (\)) /S /Span >> endobj 3773 0 obj << /Pg 5592 0 R /P 5549 0 R /K 53 /ActualText (, ) /S /Span >> endobj 3774 0 obj << /Pg 5592 0 R /P 5549 0 R /K 54 /ActualText (the ) /S /Span >> endobj 3775 0 obj << /Pg 5592 0 R /P 5549 0 R /K 55 /ActualText (expression of ) /S /Span >> endobj 3776 0 obj << /Pg 5592 0 R /P 5549 0 R /K 56 /ActualText (Muc5ac) /S /Span >> endobj 3777 0 obj << /Pg 5592 0 R /P 5549 0 R /K 57 /ActualText ( ) /S /Span >> endobj 3778 0 obj << /Pg 5592 0 R /P 5549 0 R /K 58 /ActualText (in lung tissues ) /S /Span >> endobj 3779 0 obj << /Pg 5592 0 R /P 5549 0 R /K 59 /ActualText (\() /S /Span >> endobj 3780 0 obj << /Pg 5592 0 R /P 5549 0 R /K 60 /ActualText (d) /S /Span >> endobj 3781 0 obj << /Pg 5592 0 R /P 5549 0 R /K 61 /ActualText (\)) /S /Span >> endobj 3782 0 obj << /Pg 5592 0 R /P 5549 0 R /K 62 /ActualText (, and ) /S /Span >> endobj 3783 0 obj << /Pg 5592 0 R /P 5549 0 R /K 63 /ActualText (the ) /S /Span >> endobj 3784 0 obj << /Pg 5592 0 R /P 5549 0 R /K 64 /ActualText (mucus hyperproduction in airways ) /S /Span >> endobj 3785 0 obj << /Pg 5592 0 R /P 5549 0 R /K 65 /ActualText (\() /S /Span >> endobj 3786 0 obj << /Pg 5592 0 R /P 5549 0 R /K 66 /ActualText (e) /S /Span >> endobj 3787 0 obj << /Pg 5592 0 R /P 5549 0 R /K 67 /ActualText (\)) /S /Span >> endobj 3788 0 obj << /Pg 5592 0 R /P 5549 0 R /K 68 /ActualText ( ) /S /Span >> endobj 3789 0 obj << /Pg 5592 0 R /P 5549 0 R /K 69 /ActualText (were ) /S /Span >> endobj 3790 0 obj << /Pg 5592 0 R /P 5549 0 R /K 70 /ActualText (detected. ) /S /Span >> endobj 3791 0 obj << /Pg 5592 0 R /P 5549 0 R /K 71 /ActualText (\() /S /Span >> endobj 3792 0 obj << /Pg 5592 0 R /P 5549 0 R /K 72 /ActualText (f) /S /Span >> endobj 3793 0 obj << /Pg 5592 0 R /P 5549 0 R /K 73 /ActualText (\) IL17A neutralization attenuates the inflammatory cell counts in BALF. IL17A ) /S /Span >> endobj 3794 0 obj << /Pg 5592 0 R /P 5549 0 R /K 74 /ActualText (neutralization antibody \(5 ) /S /Span >> endobj 3795 0 obj << /Pg 5592 0 R /P 5549 0 R /K 75 /ActualText (m) /S /Span >> endobj 3796 0 obj << /Pg 5592 0 R /P 5549 0 R /K 76 /ActualText (g\) was administrated intratracheally together ) /S /Span >> endobj 3797 0 obj << /Pg 5592 0 R /P 5549 0 R /K 77 /ActualText (with elastin ) /S /Span >> endobj 3798 0 obj << /Pg 5592 0 R /P 5549 0 R /K 78 /ActualText (challenge. ) /S /Span >> endobj 3799 0 obj << /Pg 5592 0 R /P 5549 0 R /K 79 /ActualText (Mac: macrophages; Neu: neutrophils; Lym: lymphocytes; Eos: eosinophils. ) /S /Span >> endobj 3800 0 obj << /Pg 5592 0 R /P 5549 0 R /K 80 /ActualText (Throughout, data are representative of 3) /S /Span >> endobj 3801 0 obj << /Pg 5592 0 R /P 5549 0 R /K 81 /ActualText (-) /S /Span >> endobj 3802 0 obj << /Pg 5592 0 R /P 5549 0 R /K 82 /ActualText (6 mice. Data are mean s.e.m. **P < 0.01, ***P < ) /S /Span >> endobj 3803 0 obj << /Pg 5592 0 R /P 5549 0 R /K 83 /ActualText (0.001 by one) /S /Span >> endobj 3804 0 obj << /Pg 5592 0 R /P 5549 0 R /K 84 /ActualText (-) /S /Span >> endobj 3805 0 obj << /Pg 5592 0 R /P 5549 0 R /K 85 /ActualText (way ANOVA.) /S /Span >> endobj 3806 0 obj << /Pg 5592 0 R /P 5549 0 R /K 86 /ActualText ( ) /S /Span >> endobj 3744 0 obj << /Pg 5592 0 R /P 5548 0 R /K 24 /ActualText (Figure 3. ) /S /Span >> endobj 3745 0 obj << /Pg 5592 0 R /P 5548 0 R /K 25 /ActualText (IL17A mediates the airway inflammation induced by CS sensitization and elastin ) /S /Span >> endobj 3746 0 obj << /Pg 5592 0 R /P 5548 0 R /K 26 /ActualText (challenge) /S /Span >> endobj 3747 0 obj << /Pg 5592 0 R /P 5548 0 R /K 27 /ActualText ( ) /S /Span >> endobj 3743 0 obj << /Pg 5592 0 R /P 5547 0 R /K 23 /ActualText ( ) /S /Span >> endobj 3720 0 obj << /Pg 5592 0 R /P 5546 0 R /K 0 /ActualText (and elastin challenge. ) /S /Span >> endobj 3721 0 obj << /Pg 5592 0 R /P 5546 0 R /K 1 /ActualText (\() /S /Span >> endobj 3722 0 obj << /Pg 5592 0 R /P 5546 0 R /K 2 /ActualText (g) /S /Span >> endobj 3723 0 obj << /Pg 5592 0 R /P 5546 0 R /K 3 /ActualText (\)) /S /Span >> endobj 3724 0 obj << /Pg 5592 0 R /P 5546 0 R /K 4 /ActualText ( ) /S /Span >> endobj 3725 0 obj << /Pg 5592 0 R /P 5546 0 R /K 5 /ActualText (Inflammatory cells in BALF. ) /S /Span >> endobj 3726 0 obj << /Pg 5592 0 R /P 5546 0 R /K 6 /ActualText (\() /S /Span >> endobj 3727 0 obj << /Pg 5592 0 R /P 5546 0 R /K 7 /ActualText (h) /S /Span >> endobj 3728 0 obj << /Pg 5592 0 R /P 5546 0 R /K 8 /ActualText (\) ) /S /Span >> endobj 3729 0 obj << /Pg 5592 0 R /P 5546 0 R /K 9 /ActualText (The concentrations of CXCL1, ) /S /Span >> endobj 3730 0 obj << /Pg 5592 0 R /P 5546 0 R /K 10 /ActualText (CXCL2, and IL6 in BALF.) /S /Span >> endobj 3731 0 obj << /Pg 5592 0 R /P 5546 0 R /K 11 /ActualText ( ) /S /Span >> endobj 3732 0 obj << /Pg 5592 0 R /P 5546 0 R /K 12 /ActualText (Mac: macrophages; Neu: neutrophils; Lym: lymphocytes; Eos) /S /Span >> endobj 3733 0 obj << /Pg 5592 0 R /P 5546 0 R /K 13 /ActualText (: ) /S /Span >> endobj 3734 0 obj << /Pg 5592 0 R /P 5546 0 R /K 14 /ActualText (eosinophils. Throughout, data are representative of 3) /S /Span >> endobj 3735 0 obj << /Pg 5592 0 R /P 5546 0 R /K 15 /ActualText (-) /S /Span >> endobj 3736 0 obj << /Pg 5592 0 R /P 5546 0 R /K 16 /ActualText (6 mice. Data are mean s.e.m. ) /S /Span >> endobj 3737 0 obj << /Pg 5592 0 R /P 5546 0 R /K 17 /ActualText (*P < ) /S /Span >> endobj 3738 0 obj << /Pg 5592 0 R /P 5546 0 R /K 18 /ActualText (0.05, ) /S /Span >> endobj 3739 0 obj << /Pg 5592 0 R /P 5546 0 R /K 19 /ActualText (**P < 0.01, ***P < 0.001 by one) /S /Span >> endobj 3740 0 obj << /Pg 5592 0 R /P 5546 0 R /K 20 /ActualText (-) /S /Span >> endobj 3741 0 obj << /Pg 5592 0 R /P 5546 0 R /K 21 /ActualText (way ANOVA.) /S /Span >> endobj 3742 0 obj << /Pg 5592 0 R /P 5546 0 R /K 22 /ActualText ( ) /S /Span >> endobj 3636 0 obj << /Pg 5591 0 R /P 5545 0 R /K 75 /ActualText (F) /S /Span >> endobj 3637 0 obj << /Pg 5591 0 R /P 5545 0 R /K 76 /ActualText (igure 2. ) /S /Span >> endobj 3638 0 obj << /Pg 5591 0 R /P 5545 0 R /K 77 /ActualText (Cigarette smoke) /S /Span >> endobj 3639 0 obj << /Pg 5591 0 R /P 5545 0 R /K 78 /ActualText (-) /S /Span >> endobj 3640 0 obj << /Pg 5591 0 R /P 5545 0 R /K 79 /ActualText (sensitized T cell immunity facilitates mice to elastin) /S /Span >> endobj 3641 0 obj << /Pg 5591 0 R /P 5545 0 R /K 80 /ActualText (-) /S /Span >> endobj 3642 0 obj << /Pg 5591 0 R /P 5545 0 R /K 81 /ActualText (induced ) /S /Span >> endobj 3643 0 obj << /Pg 5591 0 R /P 5545 0 R /K 82 /ActualText (airway inflammation) /S /Span >> endobj 3644 0 obj << /Pg 5591 0 R /P 5545 0 R /K 83 /ActualText (.) /S /Span >> endobj 3645 0 obj << /Pg 5591 0 R /P 5545 0 R /K 84 /ActualText ( ) /S /Span >> endobj 3646 0 obj << /Pg 5591 0 R /P 5545 0 R /K 85 /ActualText (\() /S /Span >> endobj 3647 0 obj << /Pg 5591 0 R /P 5545 0 R /K 86 /ActualText (a) /S /Span >> endobj 3648 0 obj << /Pg 5591 0 R /P 5545 0 R /K 87 /ActualText (-) /S /Span >> endobj 3649 0 obj << /Pg 5591 0 R /P 5545 0 R /K 88 /ActualText (c) /S /Span >> endobj 3650 0 obj << /Pg 5591 0 R /P 5545 0 R /K 89 /ActualText (\) ) /S /Span >> endobj 3651 0 obj << /Pg 5591 0 R /P 5545 0 R /K 90 /ActualText (Mice were ) /S /Span >> endobj 3652 0 obj << /Pg 5591 0 R /P 5545 0 R /K 91 /ActualText (exposed to CS for 2 weeks, and seated at room air for ) /S /Span >> endobj 3653 0 obj << /Pg 5591 0 R /P 5545 0 R /K 92 /ActualText (4, 12, or 24 weeks. Mice were then challenged with elastin \(Eln, ) /S /Span >> endobj 3654 0 obj << /Pg 5591 0 R /P 5545 0 R /K 93 /ActualText (10) /S /Span >> endobj 3655 0 obj << /Pg 5591 0 R /P 5545 0 R /K 94 /ActualText (0 ) /S /Span >> endobj 3656 0 obj << /Pg 5591 0 R /P 5545 0 R /K 95 /ActualText (m) /S /Span >> endobj 3657 0 obj << /Pg 5591 0 R /P 5545 0 R /K 96 /ActualText (g\) intratracheally \(i.t.\) ) /S /Span >> endobj 3658 0 obj << /Pg 5591 0 R /P 5545 0 R /K 97 /ActualText (once a day for 3 days and were sacrificed 48 h after the last elastin challenge.) /S /Span >> endobj 3659 0 obj << /Pg 5591 0 R /P 5545 0 R /K 98 /ActualText ( ) /S /Span >> endobj 3660 0 obj << /Pg 5591 0 R /P 5545 0 R /K 99 /ActualText (\(a\)) /S /Span >> endobj 3661 0 obj << /Pg 5591 0 R /P 5545 0 R /K 100 /ActualText ( ) /S /Span >> endobj 3662 0 obj << /Pg 5591 0 R /P 5545 0 R /K 101 /ActualText (Total ) /S /Span >> endobj 3663 0 obj << /Pg 5591 0 R /P 5545 0 R /K 102 /ActualText (inflammatory ce) /S /Span >> endobj 3664 0 obj << /Pg 5591 0 R /P 5545 0 R /K 103 /ActualText (ll and neutrophil counts in the BALF. ) /S /Span >> endobj 3665 0 obj << /Pg 5591 0 R /P 5545 0 R /K 104 /ActualText (\(b\)) /S /Span >> endobj 3666 0 obj << /Pg 5591 0 R /P 5545 0 R /K 105 /ActualText ( ) /S /Span >> endobj 3667 0 obj << /Pg 5591 0 R /P 5545 0 R /K 106 /ActualText (Representative images of H&E ) /S /Span >> endobj 3668 0 obj << /Pg 5591 0 R /P 5545 0 R /K 107 /ActualText (staining and the semi) /S /Span >> endobj 3669 0 obj << /Pg 5591 0 R /P 5545 0 R /K 108 /ActualText (-) /S /Span >> endobj 3670 0 obj << /Pg 5591 0 R /P 5545 0 R /K 109 /ActualText (quantified scoring of mouse lung sections.) /S /Span >> endobj 3671 0 obj << /Pg 5591 0 R /P 5545 0 R /K 110 /ActualText ( ) /S /Span >> endobj 3672 0 obj << /Pg 5591 0 R /P 5545 0 R /K 111 /ActualText (\(c\)) /S /Span >> endobj 3673 0 obj << /Pg 5591 0 R /P 5545 0 R /K 112 /ActualText ( ) /S /Span >> endobj 3674 0 obj << /Pg 5591 0 R /P 5545 0 R /K 113 /ActualText (Concentrations of ) /S /Span >> endobj 3675 0 obj << /Pg 5591 0 R /P 5545 0 R /K 114 /ActualText (C) /S /Span >> endobj 3676 0 obj << /Pg 5591 0 R /P 5545 0 R /K 115 /ActualText (XCL1) /S /Span >> endobj 3677 0 obj << /Pg 5591 0 R /P 5545 0 R /K 116 /ActualText ( ) /S /Span >> endobj 3678 0 obj << /Pg 5591 0 R /P 5545 0 R /K 117 /ActualText (in BALF \(left panel\) and lung tissues \(right panel\).) /S /Span >> endobj 3679 0 obj << /Pg 5591 0 R /P 5545 0 R /K 118 /ActualText ( ) /S /Span >> endobj 3680 0 obj << /Pg 5591 0 R /P 5545 0 R /K 119 /ActualText (\() /S /Span >> endobj 3681 0 obj << /Pg 5591 0 R /P 5545 0 R /K 120 /ActualText (d) /S /Span >> endobj 3682 0 obj << /Pg 5591 0 R /P 5545 0 R /K 121 /ActualText (-) /S /Span >> endobj 3683 0 obj << /Pg 5591 0 R /P 5545 0 R /K 122 /ActualText (f) /S /Span >> endobj 3684 0 obj << /Pg 5591 0 R /P 5545 0 R /K 123 /ActualText (\) CD3) /S /Span >> endobj 3685 0 obj << /Pg 5591 0 R /P 5545 0 R /K 124 /ActualText (+) /S /Span >> endobj 3686 0 obj << /Pg 5591 0 R /P 5545 0 R /K 125 /ActualText ( ) /S /Span >> endobj 3687 0 obj << /Pg 5591 0 R /P 5545 0 R /K 126 /ActualText (T cells from ) /S /Span >> endobj 3688 0 obj << /Pg 5591 0 R /P 5545 0 R /K 127 /ActualText (mediastinal lym) /S /Span >> endobj 3689 0 obj << /Pg 5591 0 R /P 5545 0 R /K 128 /ActualText (ph nodes of air controls or CS) /S /Span >> endobj 3690 0 obj << /Pg 5591 0 R /P 5545 0 R /K 129 /ActualText (-) /S /Span >> endobj 3691 0 obj << /Pg 5591 0 R /P 5545 0 R /K 130 /ActualText (sensitized mice were transferred to nave ) /S /Span >> endobj 3692 0 obj << /Pg 5591 0 R /P 5545 0 R /K 131 /ActualText (recipients) /S /Span >> endobj 3693 0 obj << /Pg 5591 0 R /P 5545 0 R /K 132 /ActualText ( ) /S /Span >> endobj 3694 0 obj << /Pg 5591 0 R /P 5545 0 R /K 133 /ActualText (and were challenged with elastin, and the inflammatory cell counts ) /S /Span >> endobj 3695 0 obj << /Pg 5591 0 R /P 5545 0 R /K 134 /ActualText (\(d\)) /S /Span >> endobj 3696 0 obj << /Pg 5591 0 R /P 5545 0 R /K 135 /ActualText (, ) /S /Span >> endobj 3697 0 obj << /Pg 5591 0 R /P 5545 0 R /K 136 /ActualText (inflammatory factors of CXCL1, CXCL2 and IL6 in BALF ) /S /Span >> endobj 3698 0 obj << /Pg 5591 0 R /P 5545 0 R /K 137 /ActualText (\(e\)) /S /Span >> endobj 3699 0 obj << /Pg 5591 0 R /P 5545 0 R /K 138 /ActualText ( ) /S /Span >> endobj 3700 0 obj << /Pg 5591 0 R /P 5545 0 R /K 139 /ActualText (and T cell responses in lung ) /S /Span >> endobj 3701 0 obj << /Pg 5591 0 R /P 5545 0 R /K 140 /ActualText (\(f\) ) /S /Span >> endobj 3702 0 obj << /Pg 5591 0 R /P 5545 0 R /K 141 /ActualText (were ) /S /Span >> endobj 3703 0 obj << /Pg 5591 0 R /P 5545 0 R /K 142 /ActualText (detected) /S /Span >> endobj 3704 0 obj << /Pg 5591 0 R /P 5545 0 R /K 143 /ActualText (.) /S /Span >> endobj 3705 0 obj << /Pg 5591 0 R /P 5545 0 R /K 144 /ActualText ( ) /S /Span >> endobj 3706 0 obj << /Pg 5591 0 R /P 5545 0 R /K 145 /ActualText (\() /S /Span >> endobj 3707 0 obj << /Pg 5591 0 R /P 5545 0 R /K 146 /ActualText (g) /S /Span >> endobj 3708 0 obj << /Pg 5591 0 R /P 5545 0 R /K 147 /ActualText (-) /S /Span >> endobj 3709 0 obj << /Pg 5591 0 R /P 5545 0 R /K 148 /ActualText (h) /S /Span >> endobj 3710 0 obj << /Pg 5591 0 R /P 5545 0 R /K 149 /ActualText (\)) /S /Span >> endobj 3711 0 obj << /Pg 5591 0 R /P 5545 0 R /K 150 /ActualText ( ) /S /Span >> endobj 3712 0 obj << /Pg 5591 0 R /P 5545 0 R /K 151 /ActualText (Rag1) /S /Span >> endobj 3713 0 obj << /Pg 5591 0 R /P 5545 0 R /K 152 /ActualText (-) /S /Span >> endobj 3714 0 obj << /Pg 5591 0 R /P 5545 0 R /K 153 /ActualText (/) /S /Span >> endobj 3715 0 obj << /Pg 5591 0 R /P 5545 0 R /K 154 /ActualText (-) /S /Span >> endobj 3716 0 obj << /Pg 5591 0 R /P 5545 0 R /K 155 /ActualText ( ) /S /Span >> endobj 3717 0 obj << /Pg 5591 0 R /P 5545 0 R /K 156 /ActualText (attenuated the airway inflammation induced by CS) /S /Span >> endobj 3718 0 obj << /Pg 5591 0 R /P 5545 0 R /K 157 /ActualText (-) /S /Span >> endobj 3719 0 obj << /Pg 5591 0 R /P 5545 0 R /K 158 /ActualText (sensitization ) /S /Span >> endobj 3635 0 obj << /Pg 5591 0 R /P 5544 0 R /K 74 /ActualText ( ) /S /Span >> endobj 3564 0 obj << /Pg 5591 0 R /P 5543 0 R /K 3 /ActualText (Figure 1. Exposure to ) /S /Span >> endobj 3565 0 obj << /Pg 5591 0 R /P 5543 0 R /K 4 /ActualText (CS sensitizes to elastin and elicits IL17A) /S /Span >> endobj 3566 0 obj << /Pg 5591 0 R /P 5543 0 R /K 5 /ActualText (-) /S /Span >> endobj 3567 0 obj << /Pg 5591 0 R /P 5543 0 R /K 6 /ActualText (predominant immune ) /S /Span >> endobj 3568 0 obj << /Pg 5591 0 R /P 5543 0 R /K 7 /ActualText (responses and bronchitis) /S /Span >> endobj 3569 0 obj << /Pg 5591 0 R /P 5543 0 R /K 8 /ActualText (-) /S /Span >> endobj 3570 0 obj << /Pg 5591 0 R /P 5543 0 R /K 9 /ActualText (like airway inflammation. \(a\)) /S /Span >> endobj 3571 0 obj << /Pg 5591 0 R /P 5543 0 R /K 10 /ActualText ( ) /S /Span >> endobj 3572 0 obj << /Pg 5591 0 R /P 5543 0 R /K 11 /ActualText (Experimental outline. Mice were ) /S /Span >> endobj 3573 0 obj << /Pg 5591 0 R /P 5543 0 R /K 12 /ActualText (exposed to CS or room air for 2 weeks, and were hosted at room air for another 2 weeks. ) /S /Span >> endobj 3574 0 obj << /Pg 5591 0 R /P 5543 0 R /K 13 /ActualText (Mice were then chal) /S /Span >> endobj 3575 0 obj << /Pg 5591 0 R /P 5543 0 R /K 14 /ActualText (lenged with elastin \(Eln, ) /S /Span >> endobj 3576 0 obj << /Pg 5591 0 R /P 5543 0 R /K 15 /ActualText (10) /S /Span >> endobj 3577 0 obj << /Pg 5591 0 R /P 5543 0 R /K 16 /ActualText (0 ) /S /Span >> endobj 3578 0 obj << /Pg 5591 0 R /P 5543 0 R /K 17 /ActualText (m) /S /Span >> endobj 3579 0 obj << /Pg 5591 0 R /P 5543 0 R /K 18 /ActualText (g\) or normal saline \(NS\) intratracheally ) /S /Span >> endobj 3580 0 obj << /Pg 5591 0 R /P 5543 0 R /K 19 /ActualText (\(i.t.\) for 3 times at day 29, 30, and 31, and were sacrificed 48 h after the last elastin challenge. ) /S /Span >> endobj 3581 0 obj << /Pg 5591 0 R /P 5543 0 R /K 20 /ActualText (For the traditional CS model, mice were exposed to CS for 3 months, as detailed in ) /S /Span >> endobj 3582 0 obj << /Pg 5591 0 R /P 5543 0 R /K 21 /ActualText (Materials ) /S /Span >> endobj 3583 0 obj << /Pg 5591 0 R /P 5543 0 R /K 22 /ActualText (and Methods. ) /S /Span >> endobj 3584 0 obj << /Pg 5591 0 R /P 5543 0 R /K 23 /ActualText (\(b\) ) /S /Span >> endobj 3585 0 obj << /Pg 5591 0 R /P 5543 0 R /K 24 /ActualText (Inflammatory cell counts in the BALF. ) /S /Span >> endobj 3586 0 obj << /Pg 5591 0 R /P 5543 0 R /K 25 /ActualText (\(c\)) /S /Span >> endobj 3587 0 obj << /Pg 5591 0 R /P 5543 0 R /K 26 /ActualText ( ) /S /Span >> endobj 3588 0 obj << /Pg 5591 0 R /P 5543 0 R /K 27 /ActualText (Representative images and ) /S /Span >> endobj 3589 0 obj << /Pg 5591 0 R /P 5543 0 R /K 28 /ActualText (semi) /S /Span >> endobj 3590 0 obj << /Pg 5591 0 R /P 5543 0 R /K 29 /ActualText (-) /S /Span >> endobj 3591 0 obj << /Pg 5591 0 R /P 5543 0 R /K 30 /ActualText (quantified 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Fig. 1b. ) /S /Span >> endobj 3624 0 obj << /Pg 5591 0 R /P 5543 0 R /K 63 /ActualText (Mac: macrophages; Neu: neutrophils; Lym: ) /S /Span >> endobj 3625 0 obj << /Pg 5591 0 R /P 5543 0 R /K 64 /ActualText (lymphocytes; Eos: eosinophils. ) /S /Span >> endobj 3626 0 obj << /Pg 5591 0 R /P 5543 0 R /K 65 /ActualText (Throughout, data are representative of 5) /S /Span >> endobj 3627 0 obj << /Pg 5591 0 R /P 5543 0 R /K 66 /ActualText (-) /S /Span >> endobj 3628 0 obj << /Pg 5591 0 R /P 5543 0 R /K 67 /ActualText (6 mice and were ) /S /Span >> endobj 3629 0 obj << /Pg 5591 0 R /P 5543 0 R /K 68 /ActualText (replicated in at least 3 ind) /S /Span >> endobj 3630 0 obj << /Pg 5591 0 R /P 5543 0 R /K 69 /ActualText (ependent experiments. 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) /S /Span >> endobj 2869 0 obj << /Pg 5587 0 R /P 5480 0 R /K 107 /ActualText (injury ) /S /Span >> endobj 2870 0 obj << /Pg 5587 0 R /P 5480 0 R /K 108 /ActualText ([3) /S /Span >> endobj 2871 0 obj << /Pg 5587 0 R /P 5480 0 R /K 109 /ActualText (2) /S /Span >> endobj 2872 0 obj << /Pg 5587 0 R /P 5480 0 R /K 110 /ActualText (-) /S /Span >> endobj 2873 0 obj << /Pg 5587 0 R /P 5480 0 R /K 111 /ActualText (3) /S /Span >> endobj 2874 0 obj << /Pg 5587 0 R /P 5480 0 R /K 112 /ActualText (4) /S /Span >> endobj 2875 0 obj << /Pg 5587 0 R /P 5480 0 R /K 113 /ActualText (]) /S /Span >> endobj 2876 0 obj << /Pg 5587 0 R /P 5480 0 R /K 114 /ActualText ( ) /S /Span >> endobj 2877 0 obj << /Pg 5587 0 R /P 5480 0 R /K 115 /ActualText (and ) /S /Span >> endobj 2878 0 obj << /Pg 5587 0 R /P 5480 0 R /K 116 /ActualText (Il17a) /S /Span >> endobj 2879 0 obj << /Pg 5587 0 R /P 5480 0 R /K 117 /ActualText (-) /S /Span >> endobj 2880 0 obj << /Pg 5587 0 R /P 5480 0 R /K 118 /ActualText (/) /S /Span >> endobj 2881 0 obj << /Pg 5587 0 R /P 5480 0 R /K 119 /ActualText (-) /S /Span >> endobj 2882 0 obj << /Pg 5587 0 R /P 5480 0 R /K 120 /ActualText ( ) /S /Span >> endobj 2883 0 obj << /Pg 5587 0 R /P 5480 0 R /K 121 /ActualText (mice exposed to CS are ) /S /Span >> endobj 2884 0 obj << /Pg 5587 0 R /P 5480 0 R /K 122 /ActualText (protected from airspace ) /S /Span >> endobj 2885 0 obj << /Pg 5587 0 R /P 5480 0 R /K 123 /ActualText (enlargement ) /S /Span >> endobj 2886 0 obj << /Pg 5587 0 R /P 5480 0 R /K 124 /ActualText ([3) /S /Span >> endobj 2887 0 obj << /Pg 5587 0 R /P 5480 0 R /K 125 /ActualText (5) /S /Span >> endobj 2888 0 obj << /Pg 5587 0 R /P 5480 0 R /K 126 /ActualText (, 3) /S /Span >> endobj 2889 0 obj << /Pg 5587 0 R /P 5480 0 R /K 127 /ActualText (6) /S /Span >> endobj 2890 0 obj << /Pg 5587 0 R /P 5480 0 R /K 128 /ActualText (]) /S /Span >> endobj 2891 0 obj << /Pg 5587 0 R /P 5480 0 R /K 129 /ActualText (. In line with these, IL17A was significantly ) /S /Span >> endobj 2892 0 obj << /Pg 5587 0 R /P 5480 0 R /K 130 /ActualText (increased in the elastin) /S /Span >> endobj 2893 0 obj << /Pg 5587 0 R /P 5480 0 R /K 131 /ActualText (-) /S /Span >> endobj 2894 0 obj << /Pg 5587 0 R /P 5480 0 R /K 132 /ActualText (driven airway inflammation) /S /Span >> endobj 2895 0 obj << /Pg 5587 0 R /P 5480 0 R /K 133 /ActualText ( ) /S /Span >> endobj 2896 0 obj << /Pg 5587 0 R /P 5480 0 R /K 134 /ActualText (in mice) /S /Span >> endobj 2897 0 obj << /Pg 5587 0 R /P 5480 0 R /K 135 /ActualText ( ) /S /Span >> endobj 2898 0 obj << /Pg 5587 0 R /P 5480 0 R /K 136 /ActualText (that were previously exposed ) /S /Span >> endobj 2899 0 obj << /Pg 5587 0 R /P 5480 0 R /K 137 /ActualText (to ) /S /Span >> endobj 2900 0 obj << /Pg 5587 0 R /P 5480 0 R /K 138 /ActualText (cigarette smoke) /S /Span >> endobj 2901 0 obj << /Pg 5587 0 R /P 5480 0 R /K 139 /ActualText (. ) /S /Span >> endobj 2902 0 obj << /Pg 5587 0 R /P 5480 0 R /K 140 /ActualText (Genetic deficiency) /S /Span >> endobj 2903 0 obj << /Pg 5587 0 R /P 5480 0 R /K 141 /ActualText ( ) /S /Span >> endobj 2904 0 obj << /Pg 5587 0 R /P 5480 0 R /K 142 /ActualText (or neutralization of IL17A decrease) /S /Span >> endobj 2905 0 obj << /Pg 5587 0 R /P 5480 0 R /K 143 /ActualText (d elastin) /S /Span >> endobj 2906 0 obj << /Pg 5587 0 R /P 5480 0 R /K 144 /ActualText (-) /S /Span >> endobj 2907 0 obj << /Pg 5587 0 R /P 5480 0 R /K 145 /ActualText (driven ) /S /Span >> endobj 2908 0 obj << /Pg 5587 0 R /P 5480 0 R /K 146 /ActualText (inflammation, indicating a pivotal role of Th17/IL17A in ) /S /Span >> endobj 2909 0 obj << /Pg 5587 0 R /P 5480 0 R /K 147 /ActualText (the ) /S /Span >> endobj 2910 0 obj << /Pg 5587 0 R /P 5480 0 R /K 148 /ActualText (pathogenesis) /S /Span >> endobj 2911 0 obj << /Pg 5587 0 R /P 5480 0 R /K 149 /ActualText ( ) /S /Span >> endobj 2912 0 obj << /Pg 5587 0 R /P 5480 0 R /K 150 /ActualText (of COPD.) /S /Span >> endobj 2913 0 obj << /Pg 5587 0 R /P 5480 0 R /K 151 /ActualText ( ) /S /Span >> endobj 2803 0 obj << /Pg 5587 0 R /P 5479 0 R /K 41 /ActualText ( ) /S /Span >> endobj 2804 0 obj << /Pg 5587 0 R /P 5479 0 R /K 42 /ActualText (It is noteworthy that COPD/emphysema generally develops decades after smoking cessation, ) /S /Span >> endobj 2805 0 obj << /Pg 5587 0 R /P 5479 0 R /K 43 /ActualText (accompanied by increased inflammation in patients  airways) /S /Span >> endobj 2806 0 obj << /Pg 5587 0 R /P 5479 0 R /K 44 /ActualText (. These observations are) /S /Span >> endobj 2807 0 obj << /Pg 5587 0 R /P 5479 0 R /K 45 /ActualText ( ) /S /Span >> endobj 2808 0 obj << /Pg 5587 0 R /P 5479 0 R /K 46 /ActualText (consistent ) /S /Span >> endobj 2809 0 obj << /Pg 5587 0 R /P 5479 0 R /K 47 /ActualText (with our results in ) /S /Span >> endobj 2810 0 obj << /Pg 5587 0 R /P 5479 0 R /K 48 /ActualText (the ) /S /Span >> endobj 2811 0 obj << /Pg 5587 0 R /P 5479 0 R /K 49 /ActualText (murine model where mice ) /S /Span >> endobj 2812 0 obj << /Pg 5587 0 R /P 5479 0 R /K 50 /ActualText (developed airway inflam) /S /Span >> endobj 2813 0 obj << /Pg 5587 0 R /P 5479 0 R /K 51 /ActualText (mation following ) /S /Span >> endobj 2814 0 obj << /Pg 5587 0 R /P 5479 0 R /K 52 /ActualText (elastin ) /S /Span >> endobj 2815 0 obj << /Pg 5587 0 R /P 5479 0 R /K 53 /ActualText (challenge 6 months after ) /S /Span >> endobj 2816 0 obj << /Pg 5587 0 R /P 5479 0 R /K 54 /ActualText (an initial) /S /Span >> endobj 2817 0 obj << /Pg 5587 0 R /P 5479 0 R /K 55 /ActualText ( ) /S /Span >> endobj 2818 0 obj << /Pg 5587 0 R /P 5479 0 R /K 56 /ActualText (2 weeks of CS exposure. Therefore, CS exposure may ) /S /Span >> endobj 2819 0 obj << /Pg 5587 0 R /P 5479 0 R /K 57 /ActualText (sensitize ) /S /Span >> endobj 2820 0 obj << /Pg 5587 0 R /P 5479 0 R /K 58 /ActualText (to elastin forming an ) /S /Span >> endobj 2821 0 obj << /Pg 5587 0 R /P 5479 0 R /K 59 /ActualText (immune memory) /S /Span >> endobj 2822 0 obj << /Pg 5587 0 R /P 5479 0 R /K 60 /ActualText ( ) /S /Span >> endobj 2823 0 obj << /Pg 5587 0 R /P 5479 0 R /K 61 /ActualText (that) /S /Span >> endobj 2824 0 obj << /Pg 5587 0 R /P 5479 0 R /K 62 /ActualText ( ) /S /Span >> endobj 2825 0 obj << /Pg 5587 0 R /P 5479 0 R /K 63 /ActualText (perpetuates inflammatory processes ) /S /Span >> endobj 2826 0 obj << /Pg 5587 0 R /P 5479 0 R /K 64 /ActualText (long ) /S /Span >> endobj 2827 0 obj << /Pg 5587 0 R /P 5479 0 R /K 65 /ActualText (after an ) /S /Span >> endobj 2828 0 obj << /Pg 5587 0 R /P 5479 0 R /K 66 /ActualText (individual ) /S /Span >> endobj 2829 0 obj << /Pg 5587 0 R /P 5479 0 R /K 67 /ActualText (quits smoking) /S /Span >> endobj 2830 0 obj << /Pg 5587 0 R /P 5479 0 R /K 68 /ActualText (.) /S /Span >> endobj 2831 0 obj << /Pg 5587 0 R /P 5479 0 R /K 69 /ActualText ( ) /S /Span >> endobj 2762 0 obj << /Pg 5587 0 R /P 5478 0 R /K 0 /ActualText (tetramers that contained ) /S /Span >> endobj 2763 0 obj << /Pg 5587 0 R /P 5478 0 R /K 1 /ActualText (elastin peptides ) /S /Span >> endobj 2764 0 obj << /Pg 5587 0 R /P 5478 0 R /K 2 /ActualText (with) /S /Span >> endobj 2765 0 obj << /Pg 5587 0 R /P 5478 0 R /K 3 /ActualText ( ) /S /Span >> endobj 2766 0 obj << /Pg 5587 0 R /P 5478 0 R /K 4 /ActualText (th) /S /Span >> endobj 2767 0 obj << /Pg 5587 0 R /P 5478 0 R /K 5 /ActualText (ese) /S /Span >> endobj 2768 0 obj << /Pg 5587 0 R /P 5478 0 R /K 6 /ActualText ( ) /S /Span >> endobj 2769 0 obj << /Pg 5587 0 R /P 5478 0 R /K 7 /ActualText (motif) /S /Span >> endobj 2770 0 obj << /Pg 5587 0 R /P 5478 0 R /K 8 /ActualText (s) /S /Span >> endobj 2771 0 obj << /Pg 5587 0 R /P 5478 0 R /K 9 /ActualText ( ) /S /Span >> endobj 2772 0 obj << /Pg 5587 0 R /P 5478 0 R /K 10 /ActualText (were) /S /Span >> endobj 2773 0 obj << /Pg 5587 0 R /P 5478 0 R /K 11 /ActualText ( ) /S /Span >> endobj 2774 0 obj << /Pg 5587 0 R /P 5478 0 R /K 12 /ActualText (able to direct) /S /Span >> endobj 2775 0 obj << /Pg 5587 0 R /P 5478 0 R /K 13 /ActualText (ly ) /S /Span >> endobj 2776 0 obj << /Pg 5587 0 R /P 5478 0 R /K 14 /ActualText (bind) /S /Span >> endobj 2777 0 obj << /Pg 5587 0 R /P 5478 0 R /K 15 /ActualText ( ) /S /Span >> endobj 2778 0 obj << /Pg 5587 0 R /P 5478 0 R /K 16 /ActualText (T cells from ) /S /Span >> endobj 2779 0 obj << /Pg 5587 0 R /P 5478 0 R /K 17 /ActualText (COPD patients) /S /Span >> endobj 2780 0 obj << /Pg 5587 0 R /P 5478 0 R /K 18 /ActualText ( ) /S /Span >> endobj 2781 0 obj << /Pg 5587 0 R /P 5478 0 R /K 19 /ActualText (and induce airway inflammation in CS) /S /Span >> endobj 2782 0 obj << /Pg 5587 0 R /P 5478 0 R /K 20 /ActualText (-) /S /Span >> endobj 2783 0 obj << /Pg 5587 0 R /P 5478 0 R /K 21 /ActualText (sensitized mice. ) /S /Span >> endobj 2784 0 obj << /Pg 5587 0 R /P 5478 0 R /K 22 /ActualText (We further show that ) /S /Span >> endobj 2785 0 obj << /Pg 5587 0 R /P 5478 0 R /K 23 /ActualText (antibodies that target the active motif in elastin ) /S /Span >> endobj 2786 0 obj << /Pg 5587 0 R /P 5478 0 R /K 24 /ActualText (effectively attenuat) /S /Span >> endobj 2787 0 obj << /Pg 5587 0 R /P 5478 0 R /K 25 /ActualText (ed) /S /Span >> endobj 2788 0 obj << /Pg 5587 0 R /P 5478 0 R /K 26 /ActualText ( ) /S /Span >> endobj 2789 0 obj << /Pg 5587 0 R /P 5478 0 R /K 27 /ActualText (airway inflammation ) /S /Span >> endobj 2790 0 obj << /Pg 5587 0 R /P 5478 0 R /K 28 /ActualText (when ) /S /Span >> endobj 2791 0 obj << /Pg 5587 0 R /P 5478 0 R /K 29 /ActualText (delivered during cigarette smoke exposure. ) /S /Span >> endobj 2792 0 obj << /Pg 5587 0 R /P 5478 0 R /K 30 /ActualText (Thus) /S /Span >> endobj 2793 0 obj << /Pg 5587 0 R /P 5478 0 R /K 31 /ActualText (, the MMP12) /S /Span >> endobj 2794 0 obj << /Pg 5587 0 R /P 5478 0 R /K 32 /ActualText (-) /S /Span >> endobj 2795 0 obj << /Pg 5587 0 R /P 5478 0 R /K 33 /ActualText (generated elastin peptides induced ) /S /Span >> endobj 2796 0 obj << /Pg 5587 0 R /P 5478 0 R /K 34 /ActualText (by CS exposure not only are chemotactic factors for monocytes, but also initiate T cell responses, ) /S /Span >> endobj 2797 0 obj << /Pg 5587 0 R /P 5478 0 R /K 35 /ActualText (thereby driving subsequent airway inflammation) /S /Span >> endobj 2798 0 obj << /Pg 5587 0 R /P 5478 0 R /K 36 /ActualText (, ) /S /Span >> endobj 2799 0 obj << /Pg 5587 0 R /P 5478 0 R /K 37 /ActualText (mucus production) /S /Span >> endobj 2800 0 obj << /Pg 5587 0 R /P 5478 0 R /K 38 /ActualText (, and airspace enlargement) /S /Span >> endobj 2801 0 obj << /Pg 5587 0 R /P 5478 0 R /K 39 /ActualText (. ) /S /Span >> endobj 2802 0 obj << /Pg 5587 0 R /P 5478 0 R /K 40 /ActualText ( ) /S /Span >> endobj 2688 0 obj << /Pg 5586 0 R /P 5477 0 R /K 135 /ActualText ( ) /S /Span >> endobj 2689 0 obj << /Pg 5586 0 R /P 5477 0 R /K 136 /ActualText (Our current study emphasizes the pivotal role of MMP12 in initiating CS) /S /Span >> endobj 2690 0 obj << /Pg 5586 0 R /P 5477 0 R /K 137 /ActualText (-) /S /Span >> endobj 2691 0 obj << /Pg 5586 0 R /P 5477 0 R /K 138 /ActualText (induced autoimmune ) /S /Span >> endobj 2692 0 obj << /Pg 5586 0 R /P 5477 0 R /K 139 /ActualText (responses. In line with previous report) /S /Span >> endobj 2693 0 obj << /Pg 5586 0 R /P 5477 0 R /K 140 /ActualText (s) /S /Span >> endobj 2694 0 obj << /Pg 5586 0 R /P 5477 0 R /K 141 /ActualText ( ) /S /Span >> endobj 2695 0 obj << /Pg 5586 0 R /P 5477 0 R /K 142 /ActualText (showing that ) /S /Span >> endobj 2696 0 obj << /Pg 5586 0 R /P 5477 0 R /K 143 /ActualText (Mmp12) /S /Span >> endobj 2697 0 obj << /Pg 5586 0 R /P 5477 0 R /K 144 /ActualText (-) /S /Span >> endobj 2698 0 obj << /Pg 5586 0 R /P 5477 0 R /K 145 /ActualText (/) /S /Span >> endobj 2699 0 obj << /Pg 5586 0 R /P 5477 0 R /K 146 /ActualText (-) /S /Span >> endobj 2700 0 obj << /Pg 5586 0 R /P 5477 0 R /K 147 /ActualText ( ) /S /Span >> endobj 2701 0 obj << /Pg 5586 0 R /P 5477 0 R /K 148 /ActualText (mice are protected from ) /S /Span >> endobj 2702 0 obj << /Pg 5586 0 R /P 5477 0 R /K 149 /ActualText (CS) /S /Span >> endobj 2703 0 obj << /Pg 5586 0 R /P 5477 0 R /K 150 /ActualText (-) /S /Span >> endobj 2704 0 obj << /Pg 5586 0 R /P 5477 0 R /K 151 /ActualText (induced airway inflammation and ) /S /Span >> endobj 2705 0 obj << /Pg 5586 0 R /P 5477 0 R /K 152 /ActualText (emphysema ) /S /Span >> endobj 2706 0 obj << /Pg 5586 0 R /P 5477 0 R /K 153 /ActualText ([) /S /Span >> endobj 2707 0 obj << /Pg 5586 0 R /P 5477 0 R /K 154 /ActualText (15) /S /Span >> endobj 2708 0 obj << /Pg 5586 0 R /P 5477 0 R /K 155 /ActualText (]) /S /Span >> endobj 2709 0 obj << /Pg 5586 0 R /P 5477 0 R /K 156 /ActualText (, ) /S /Span >> endobj 2710 0 obj << /Pg 5586 0 R /P 5477 0 R /K 157 /ActualText (Mmp12) /S /Span >> endobj 2711 0 obj << /Pg 5586 0 R /P 5477 0 R /K 158 /ActualText ( ) /S /Span >> endobj 2712 0 obj << /Pg 5586 0 R /P 5477 0 R /K 159 /ActualText (deficiency ) /S /Span >> endobj 2713 0 obj << /Pg 5586 0 R /P 5477 0 R /K 160 /ActualText (markedly ) /S /Span >> endobj 2714 0 obj << /Pg 5586 0 R /P 5477 0 R /K 161 /ActualText (attenuated) /S /Span >> endobj 2715 0 obj << /Pg 5586 0 R /P 5477 0 R /K 162 /ActualText ( ) /S /Span >> endobj 2716 0 obj << /Pg 5586 0 R /P 5477 0 R /K 163 /ActualText (elastin) /S /Span >> endobj 2717 0 obj << /Pg 5586 0 R /P 5477 0 R /K 164 /ActualText (-) /S /Span >> endobj 2718 0 obj << /Pg 5586 0 R /P 5477 0 R /K 165 /ActualText (induced airway inflammation and mucus hyperproduction) /S /Span >> endobj 2719 0 obj << /Pg 5586 0 R /P 5477 0 R /K 166 /ActualText ( ) /S /Span >> endobj 2720 0 obj << /Pg 5586 0 R /P 5477 0 R /K 167 /ActualText (in mice with prior exposure to ) /S /Span >> endobj 2721 0 obj << /Pg 5586 0 R /P 5477 0 R /K 168 /ActualText (cigarette smoke) /S /Span >> endobj 2722 0 obj << /Pg 5586 0 R /P 5477 0 R /K 169 /ActualText (. ) /S /Span >> endobj 2723 0 obj << /Pg 5586 0 R /P 5477 0 R /K 170 /ActualText (Other MMPs in ) /S /Span >> endobj 2724 0 obj << /Pg 5586 0 R /P 5477 0 R /K 171 /ActualText (Mmp12) /S /Span >> endobj 2725 0 obj << /Pg 5586 0 R /P 5477 0 R /K 172 /ActualText (-) /S /Span >> endobj 2726 0 obj << /Pg 5586 0 R /P 5477 0 R /K 173 /ActualText (/) /S /Span >> endobj 2727 0 obj << /Pg 5586 0 R /P 5477 0 R /K 174 /ActualText (-) /S /Span >> endobj 2728 0 obj << /Pg 5586 0 R /P 5477 0 R /K 175 /ActualText ( ) /S /Span >> endobj 2729 0 obj << /Pg 5586 0 R /P 5477 0 R /K 176 /ActualText (alveolar macrophages were not altered \(data not ) /S /Span >> endobj 2730 0 obj << /Pg 5586 0 R /P 5477 0 R /K 177 /ActualText (shown\), and thus we could not rule out) /S /Span >> endobj 2731 0 obj << /Pg 5586 0 R /P 5477 0 R /K 178 /ActualText ( ) /S /Span >> endobj 2732 0 obj << /Pg 5586 0 R /P 5477 0 R /K 179 /ActualText (the functions of other MMPs in our study. ) /S /Span >> endobj 2733 0 obj << /Pg 5586 0 R /P 5477 0 R /K 180 /ActualText (In fact, ) /S /Span >> endobj 2734 0 obj << /Pg 5586 0 R /P 5477 0 R /K 181 /ActualText (MMP12 ) /S /Span >> endobj 2735 0 obj << /Pg 5586 0 R /P 5477 0 R /K 182 /ActualText (deficiency only partially attenuated inflammatory processes) /S /Span >> endobj 2736 0 obj << /Pg 5586 0 R /P 5477 0 R /K 183 /ActualText (, supporting ) /S /Span >> endobj 2737 0 obj << /Pg 5586 0 R /P 5477 0 R /K 184 /ActualText (the participation of) /S /Span >> endobj 2738 0 obj << /Pg 5586 0 R /P 5477 0 R /K 185 /ActualText ( ) /S /Span >> endobj 2739 0 obj << /Pg 5586 0 R /P 5477 0 R /K 186 /ActualText (other ) /S /Span >> endobj 2740 0 obj << /Pg 5586 0 R /P 5477 0 R /K 187 /ActualText (MMPs in CS) /S /Span >> endobj 2741 0 obj << /Pg 5586 0 R /P 5477 0 R /K 188 /ActualText (-) /S /Span >> endobj 2742 0 obj << /Pg 5586 0 R /P 5477 0 R /K 189 /ActualText (induced autoimmunity.) /S /Span >> endobj 2743 0 obj << /Pg 5586 0 R /P 5477 0 R /K 190 /ActualText ( ) /S /Span >> endobj 2744 0 obj << /Pg 5586 0 R /P 5477 0 R /K 191 /ActualText (One of the mechanisms ) /S /Span >> endobj 2745 0 obj << /Pg 5586 0 R /P 5477 0 R /K 192 /ActualText (that implicates) /S /Span >> endobj 2746 0 obj << /Pg 5586 0 R /P 5477 0 R /K 193 /ActualText ( ) /S /Span >> endobj 2747 0 obj << /Pg 5586 0 R /P 5477 0 R /K 194 /ActualText (MMP12 in ) /S /Span >> endobj 2748 0 obj << /Pg 5586 0 R /P 5477 0 R /K 195 /ActualText (the ) /S /Span >> endobj 2749 0 obj << /Pg 5586 0 R /P 5477 0 R /K 196 /ActualText (pathogenesis of ) /S /Span >> endobj 2750 0 obj << /Pg 5586 0 R /P 5477 0 R /K 197 /ActualText (COPD is the production of elastin fragments ) /S /Span >> endobj 2751 0 obj << /Pg 5586 0 R /P 5477 0 R /K 198 /ActualText (that ) /S /Span >> endobj 2752 0 obj << /Pg 5586 0 R /P 5477 0 R /K 199 /ActualText (contain GXXPG or XGXPG ) /S /Span >> endobj 2753 0 obj << /Pg 5586 0 R /P 5477 0 R /K 200 /ActualText (moti) /S /Span >> endobj 2754 0 obj << /Pg 5586 0 R /P 5477 0 R /K 201 /ActualText (fs) /S /Span >> endobj 2755 0 obj << /Pg 5586 0 R /P 5477 0 R /K 202 /ActualText (, ) /S /Span >> endobj 2756 0 obj << /Pg 5586 0 R /P 5477 0 R /K 203 /ActualText (which exert monocyte chemotactic ) /S /Span >> endobj 2757 0 obj << /Pg 5586 0 R /P 5477 0 R /K 204 /ActualText (activity ) /S /Span >> endobj 2758 0 obj << /Pg 5586 0 R /P 5477 0 R /K 205 /ActualText ([) /S /Span >> endobj 2759 0 obj << /Pg 5586 0 R /P 5477 0 R /K 206 /ActualText (17) /S /Span >> endobj 2760 0 obj << /Pg 5586 0 R /P 5477 0 R /K 207 /ActualText (]) /S /Span >> endobj 2761 0 obj << /Pg 5586 0 R /P 5477 0 R /K 208 /ActualText (. In our current study, we found that ) /S /Span >> endobj 2608 0 obj << /Pg 5586 0 R /P 5476 0 R /K 55 /ActualText ( ) /S /Span >> endobj 2609 0 obj << /Pg 5586 0 R /P 5476 0 R /K 56 /ActualText (Airway mucus hypersecretion is one of the cardinal features for both COPD and asthma. ) /S /Span >> endobj 2610 0 obj << /Pg 5586 0 R /P 5476 0 R /K 57 /ActualText (However, unlike i) /S /Span >> endobj 2611 0 obj << /Pg 5586 0 R /P 5476 0 R /K 58 /ActualText (ncreased mucus hyperproduction in asthma models) /S /Span >> endobj 2612 0 obj << /Pg 5586 0 R /P 5476 0 R /K 59 /ActualText ( ) /S /Span >> endobj 2613 0 obj << /Pg 5586 0 R /P 5476 0 R /K 60 /ActualText (or by particulate matter ) /S /Span >> endobj 2614 0 obj << /Pg 5586 0 R /P 5476 0 R /K 61 /ActualText ([2) /S /Span >> endobj 2615 0 obj << /Pg 5586 0 R /P 5476 0 R /K 62 /ActualText (2) /S /Span >> endobj 2616 0 obj << /Pg 5586 0 R /P 5476 0 R /K 63 /ActualText (-) /S /Span >> endobj 2617 0 obj << /Pg 5586 0 R /P 5476 0 R /K 64 /ActualText (2) /S /Span >> endobj 2618 0 obj << /Pg 5586 0 R /P 5476 0 R /K 65 /ActualText (4) /S /Span >> endobj 2619 0 obj << /Pg 5586 0 R /P 5476 0 R /K 66 /ActualText (]) /S /Span >> endobj 2620 0 obj << /Pg 5586 0 R /P 5476 0 R /K 67 /ActualText (, ) /S /Span >> endobj 2621 0 obj << /Pg 5586 0 R /P 5476 0 R /K 68 /ActualText (there is ) /S /Span >> endobj 2622 0 obj << /Pg 5586 0 R /P 5476 0 R /K 69 /ActualText (little ) /S /Span >> endobj 2623 0 obj << /Pg 5586 0 R /P 5476 0 R /K 70 /ActualText (evidence that) /S /Span >> endobj 2624 0 obj << /Pg 5586 0 R /P 5476 0 R /K 71 /ActualText ( ) /S /Span >> endobj 2625 0 obj << /Pg 5586 0 R /P 5476 0 R /K 72 /ActualText (CS exposure) /S /Span >> endobj 2626 0 obj << /Pg 5586 0 R /P 5476 0 R /K 73 /ActualText ( ) /S /Span >> endobj 2627 0 obj << /Pg 5586 0 R /P 5476 0 R /K 74 /ActualText (increases mucus production in mouse models) /S /Span >> endobj 2628 0 obj << /Pg 5586 0 R /P 5476 0 R /K 75 /ActualText (. ) /S /Span >> endobj 2629 0 obj << /Pg 5586 0 R /P 5476 0 R /K 76 /ActualText (In ) /S /Span >> endobj 2630 0 obj << /Pg 5586 0 R /P 5476 0 R /K 77 /ActualText (contrast, a) /S /Span >> endobj 2631 0 obj << /Pg 5586 0 R /P 5476 0 R /K 78 /ActualText (crolein, a reactive ) /S /Span >> endobj 2632 0 obj << /Pg 5586 0 R /P 5476 0 R /K 79 /ActualText (, ) /S /Span >> endobj 2633 0 obj << /Pg 5586 0 R /P 5476 0 R /K 80 /ActualText (-) /S /Span >> endobj 2634 0 obj << /Pg 5586 0 R /P 5476 0 R /K 81 /ActualText (unsaturated aldehyde) /S /Span >> endobj 2635 0 obj << /Pg 5586 0 R /P 5476 0 R /K 82 /ActualText ( ) /S /Span >> endobj 2636 0 obj << /Pg 5586 0 R /P 5476 0 R /K 83 /ActualText (and) /S /Span >> endobj 2637 0 obj << /Pg 5586 0 R /P 5476 0 R /K 84 /ActualText ( ) /S /Span >> endobj 2638 0 obj << /Pg 5586 0 R /P 5476 0 R /K 85 /ActualText (component of cigarette smoke, ) /S /Span >> endobj 2639 0 obj << /Pg 5586 0 R /P 5476 0 R /K 86 /ActualText (readily) /S /Span >> endobj 2640 0 obj << /Pg 5586 0 R /P 5476 0 R /K 87 /ActualText ( ) /S /Span >> endobj 2641 0 obj << /Pg 5586 0 R /P 5476 0 R /K 88 /ActualText (induce) /S /Span >> endobj 2642 0 obj << /Pg 5586 0 R /P 5476 0 R /K 89 /ActualText (s) /S /Span >> endobj 2643 0 obj << /Pg 5586 0 R /P 5476 0 R /K 90 /ActualText ( ) /S /Span >> endobj 2644 0 obj << /Pg 5586 0 R /P 5476 0 R /K 91 /ActualText (mucus production in murine ) /S /Span >> endobj 2645 0 obj << /Pg 5586 0 R /P 5476 0 R /K 92 /ActualText (airways ) /S /Span >> endobj 2646 0 obj << /Pg 5586 0 R /P 5476 0 R /K 93 /ActualText ([2) /S /Span >> endobj 2647 0 obj << /Pg 5586 0 R /P 5476 0 R /K 94 /ActualText (5) /S /Span >> endobj 2648 0 obj << /Pg 5586 0 R /P 5476 0 R /K 95 /ActualText (, 2) /S /Span >> endobj 2649 0 obj << /Pg 5586 0 R /P 5476 0 R /K 96 /ActualText (6) /S /Span >> endobj 2650 0 obj << /Pg 5586 0 R /P 5476 0 R /K 97 /ActualText (]) /S /Span >> endobj 2651 0 obj << /Pg 5586 0 R /P 5476 0 R /K 98 /ActualText (. Our findings identify elastin as an effective ) /S /Span >> endobj 2652 0 obj << /Pg 5586 0 R /P 5476 0 R /K 99 /ActualText (stimulator to trigger mucin expression in CS) /S /Span >> endobj 2653 0 obj << /Pg 5586 0 R /P 5476 0 R /K 100 /ActualText (-) /S /Span >> endobj 2654 0 obj << /Pg 5586 0 R /P 5476 0 R /K 101 /ActualText (exposed) /S /Span >> endobj 2655 0 obj << /Pg 5586 0 R /P 5476 0 R /K 102 /ActualText ( ) /S /Span >> endobj 2656 0 obj << /Pg 5586 0 R /P 5476 0 R /K 103 /ActualText (mice) /S /Span >> endobj 2657 0 obj << /Pg 5586 0 R /P 5476 0 R /K 104 /ActualText ( ) /S /Span >> endobj 2658 0 obj << /Pg 5586 0 R /P 5476 0 R /K 105 /ActualText (and) /S /Span >> endobj 2659 0 obj << /Pg 5586 0 R /P 5476 0 R /K 106 /ActualText (,) /S /Span >> endobj 2660 0 obj << /Pg 5586 0 R /P 5476 0 R /K 107 /ActualText ( ) /S /Span >> endobj 2661 0 obj << /Pg 5586 0 R /P 5476 0 R /K 108 /ActualText (thus) /S /Span >> endobj 2662 0 obj << /Pg 5586 0 R /P 5476 0 R /K 109 /ActualText (,) /S /Span >> endobj 2663 0 obj << /Pg 5586 0 R /P 5476 0 R /K 110 /ActualText ( ) /S /Span >> endobj 2664 0 obj << /Pg 5586 0 R /P 5476 0 R /K 111 /ActualText (provide ) /S /Span >> endobj 2665 0 obj << /Pg 5586 0 R /P 5476 0 R /K 112 /ActualText (a novel) /S /Span >> endobj 2666 0 obj << /Pg 5586 0 R /P 5476 0 R /K 113 /ActualText ( ) /S /Span >> endobj 2667 0 obj << /Pg 5586 0 R /P 5476 0 R /K 114 /ActualText (model for ) /S /Span >> endobj 2668 0 obj << /Pg 5586 0 R /P 5476 0 R /K 115 /ActualText (mucus research) /S /Span >> endobj 2669 0 obj << /Pg 5586 0 R /P 5476 0 R /K 116 /ActualText ( ) /S /Span >> endobj 2670 0 obj << /Pg 5586 0 R /P 5476 0 R /K 117 /ActualText (with relevance to COPD) /S /Span >> endobj 2671 0 obj << /Pg 5586 0 R /P 5476 0 R /K 118 /ActualText (. In fact, we have observed that elastin peptides ) /S /Span >> endobj 2672 0 obj << /Pg 5586 0 R /P 5476 0 R /K 119 /ActualText (containing the active GXXPG conformational ) /S /Span >> endobj 2673 0 obj << /Pg 5586 0 R /P 5476 0 R /K 120 /ActualText (motif ) /S /Span >> endobj 2674 0 obj << /Pg 5586 0 R /P 5476 0 R /K 121 /ActualText ([2) /S /Span >> endobj 2675 0 obj << /Pg 5586 0 R /P 5476 0 R /K 122 /ActualText (7) /S /Span >> endobj 2676 0 obj << /Pg 5586 0 R /P 5476 0 R /K 123 /ActualText (, ) /S /Span >> endobj 2677 0 obj << /Pg 5586 0 R /P 5476 0 R /K 124 /ActualText (2) /S /Span >> endobj 2678 0 obj << /Pg 5586 0 R /P 5476 0 R /K 125 /ActualText (8) /S /Span >> endobj 2679 0 obj << /Pg 5586 0 R /P 5476 0 R /K 126 /ActualText (]) /S /Span >> endobj 2680 0 obj << /Pg 5586 0 R /P 5476 0 R /K 127 /ActualText (, but not elastin protein, induced ) /S /Span >> endobj 2681 0 obj << /Pg 5586 0 R /P 5476 0 R /K 128 /ActualText (MUC5AC expression in human bronchial epithelial cells \(data not shown\), suggesting a critical ) /S /Span >> endobj 2682 0 obj << /Pg 5586 0 R /P 5476 0 R /K 129 /ActualText (role ) /S /Span >> endobj 2683 0 obj << /Pg 5586 0 R /P 5476 0 R /K 130 /ActualText (of these active peptides in driving mucus production) /S /Span >> endobj 2684 0 obj << /Pg 5586 0 R /P 5476 0 R /K 131 /ActualText ( ) /S /Span >> endobj 2685 0 obj << /Pg 5586 0 R /P 5476 0 R /K 132 /ActualText (in COPD) /S /Span >> endobj 2686 0 obj << /Pg 5586 0 R /P 5476 0 R /K 133 /ActualText (.) /S /Span >> endobj 2687 0 obj << /Pg 5586 0 R /P 5476 0 R /K 134 /ActualText ( ) /S /Span >> endobj 2553 0 obj << /Pg 5586 0 R /P 5475 0 R /K 0 /ActualText (model ) /S /Span >> endobj 2554 0 obj << /Pg 5586 0 R /P 5475 0 R /K 1 /ActualText (presented here ) /S /Span >> endobj 2555 0 obj << /Pg 5586 0 R /P 5475 0 R /K 2 /ActualText (overcomes these ) /S /Span >> endobj 2556 0 obj << /Pg 5586 0 R /P 5475 0 R /K 3 /ActualText (limitations) /S /Span >> endobj 2557 0 obj << /Pg 5586 0 R /P 5475 0 R /K 4 /ActualText (. We observed) /S /Span >> endobj 2558 0 obj << /Pg 5586 0 R /P 5475 0 R /K 5 /ActualText ( ) /S /Span >> endobj 2559 0 obj << /Pg 5586 0 R /P 5475 0 R /K 6 /ActualText (moderate levels of neutrophilic ) /S /Span >> endobj 2560 0 obj << /Pg 5586 0 R /P 5475 0 R /K 7 /ActualText (airway inflammation \(30) /S /Span >> endobj 2561 0 obj << /Pg 5586 0 R /P 5475 0 R /K 8 /ActualText (-) /S /Span >> endobj 2562 0 obj << /Pg 5586 0 R /P 5475 0 R /K 9 /ActualText (60% of neutrophils in BALF, comparable with proportion) /S /Span >> endobj 2563 0 obj << /Pg 5586 0 R /P 5475 0 R /K 10 /ActualText (s) /S /Span >> endobj 2564 0 obj << /Pg 5586 0 R /P 5475 0 R /K 11 /ActualText ( ) /S /Span >> endobj 2565 0 obj << /Pg 5586 0 R /P 5475 0 R /K 12 /ActualText (of ) /S /Span >> endobj 2566 0 obj << /Pg 5586 0 R /P 5475 0 R /K 13 /ActualText (eosinophils in asthma model) /S /Span >> endobj 2567 0 obj << /Pg 5586 0 R /P 5475 0 R /K 14 /ActualText (s) /S /Span >> endobj 2568 0 obj << /Pg 5586 0 R /P 5475 0 R /K 15 /ActualText (\), ) /S /Span >> endobj 2569 0 obj << /Pg 5586 0 R /P 5475 0 R /K 16 /ActualText (a ) /S /Span >> endobj 2570 0 obj << /Pg 5586 0 R /P 5475 0 R /K 17 /ActualText (Th1/Th17 ) /S /Span >> endobj 2571 0 obj << /Pg 5586 0 R /P 5475 0 R /K 18 /ActualText (inflammatory signature) /S /Span >> endobj 2572 0 obj << /Pg 5586 0 R /P 5475 0 R /K 19 /ActualText (, marked mucus ) /S /Span >> endobj 2573 0 obj << /Pg 5586 0 R /P 5475 0 R /K 20 /ActualText (hyperproduction, increased airway rem) /S /Span >> endobj 2574 0 obj << /Pg 5586 0 R /P 5475 0 R /K 21 /ActualText (odelling, declined lung function, and enlarged airspace) /S /Span >> endobj 2575 0 obj << /Pg 5586 0 R /P 5475 0 R /K 22 /ActualText (s) /S /Span >> endobj 2576 0 obj << /Pg 5586 0 R /P 5475 0 R /K 23 /ActualText (. ) /S /Span >> endobj 2577 0 obj << /Pg 5586 0 R /P 5475 0 R /K 24 /ActualText (It ) /S /Span >> endobj 2578 0 obj << /Pg 5586 0 R /P 5475 0 R /K 25 /ActualText (required 1 month to establish ) /S /Span >> endobj 2579 0 obj << /Pg 5586 0 R /P 5475 0 R /K 26 /ActualText (airway inflammation) /S /Span >> endobj 2580 0 obj << /Pg 5586 0 R /P 5475 0 R /K 27 /ActualText (,) /S /Span >> endobj 2581 0 obj << /Pg 5586 0 R /P 5475 0 R /K 28 /ActualText ( ) /S /Span >> endobj 2582 0 obj << /Pg 5586 0 R /P 5475 0 R /K 29 /ActualText (mimicking human bronchitis) /S /Span >> endobj 2583 0 obj << /Pg 5586 0 R /P 5475 0 R /K 30 /ActualText (,) /S /Span >> endobj 2584 0 obj << /Pg 5586 0 R /P 5475 0 R /K 31 /ActualText ( ) /S /Span >> endobj 2585 0 obj << /Pg 5586 0 R /P 5475 0 R /K 32 /ActualText (and 2 months ) /S /Span >> endobj 2586 0 obj << /Pg 5586 0 R /P 5475 0 R /K 33 /ActualText (to ) /S /Span >> endobj 2587 0 obj << /Pg 5586 0 R /P 5475 0 R /K 34 /ActualText (establish ) /S /Span >> endobj 2588 0 obj << /Pg 5586 0 R /P 5475 0 R /K 35 /ActualText (parenchymal damage. In addition, our model provides ) /S /Span >> endobj 2589 0 obj << /Pg 5586 0 R /P 5475 0 R /K 36 /ActualText ( proof of concept  that ) /S /Span >> endobj 2590 0 obj << /Pg 5586 0 R /P 5475 0 R /K 37 /ActualText (autoimmune pro) /S /Span >> endobj 2591 0 obj << /Pg 5586 0 R /P 5475 0 R /K 38 /ActualText (cesses are induced by cigarette smoke) /S /Span >> endobj 2592 0 obj << /Pg 5586 0 R /P 5475 0 R /K 39 /ActualText ( ) /S /Span >> endobj 2593 0 obj << /Pg 5586 0 R /P 5475 0 R /K 40 /ActualText (exposure and may provide a ) /S /Span >> endobj 2594 0 obj << /Pg 5586 0 R /P 5475 0 R /K 41 /ActualText (novel ) /S /Span >> endobj 2595 0 obj << /Pg 5586 0 R /P 5475 0 R /K 42 /ActualText (framework ) /S /Span >> endobj 2596 0 obj << /Pg 5586 0 R /P 5475 0 R /K 43 /ActualText (for building animal models for ) /S /Span >> endobj 2597 0 obj << /Pg 5586 0 R /P 5475 0 R /K 44 /ActualText (other) /S /Span >> endobj 2598 0 obj << /Pg 5586 0 R /P 5475 0 R /K 45 /ActualText ( ) /S /Span >> endobj 2599 0 obj << /Pg 5586 0 R /P 5475 0 R /K 46 /ActualText (autoimmune diseases) /S /Span >> endobj 2600 0 obj << /Pg 5586 0 R /P 5475 0 R /K 47 /ActualText (.) /S /Span >> endobj 2601 0 obj << /Pg 5586 0 R /P 5475 0 R /K 48 /ActualText ( ) /S /Span >> endobj 2602 0 obj << /Pg 5586 0 R /P 5475 0 R /K 49 /ActualText (This is of clinical relevance, ) /S /Span >> endobj 2603 0 obj << /Pg 5586 0 R /P 5475 0 R /K 50 /ActualText (as cigarette smoke exposure is a known risk factor for a number of autoimmune diseases, ) /S /Span >> endobj 2604 0 obj << /Pg 5586 0 R /P 5475 0 R /K 51 /ActualText (such as) /S /Span >> endobj 2605 0 obj << /Pg 5586 0 R /P 5475 0 R /K 52 /ActualText ( ) /S /Span >> endobj 2606 0 obj << /Pg 5586 0 R /P 5475 0 R /K 53 /ActualText (rheumatoid arthritis. ) /S /Span >> endobj 2607 0 obj << /Pg 5586 0 R /P 5475 0 R /K 54 /ActualText ( ) /S /Span >> endobj 5585 0 obj << /Group << /CS /DeviceRGB /Type /Group /S /Transparency >> /StructParents 12 /Parent 7 0 R /Resources << /Font << /F2 5029 0 R /F1 5026 0 R /F4 5034 0 R >> /ProcSet [/PDF /Text /ImageB /ImageC /ImageI] >> /MediaBox [0 0 595.44 842.04] /Tabs /S /Type /Page /Contents 5613 0 R >> endobj 5613 0 obj << /Filter /FlateDecode /Length 5791 >> stream x]Y~0/foyv7`H#F7vdQvׇųk],d^?˫oonvݷ^}ًNֻ?'v8zMFF^zl?f1o[Ĵ;\e;{bJc*!liS)7)FӲ>\-0#oa`2ԸS|?|>H38~  5اHϞNƑsOoϑDz^=1[+)SW". uaK!^zyrϖdJAT"jQK"'♸=%Ié#?@aPΨ VfXGz>֐ ;ba/PqI$()Ǐh{a>L*UK=H5ѳrwV!& wlr J %{J1z&|g=}PȄgbGBhCQ{3a[(f7'c<$)8sPrSW!YpE`1CsV$4I dE62Idr:^mZj[I &]v#KLJ?5IY:SV:U83W%WE.odƧ MnUrsVA Jrm7`r$1>-Z'xFzag4jg| x_lٛ 8"Zd) V!v 1+H3-}A?ug0,K"ո{E dIDQ!M)/Z7`)$L ;8SHQ9k4 'Þ]YԢt2~@Rl#a'a=)#`JxhHۆʮ1 `-Z E3Zly'Ma fղ{S`^3:(.V"( zc FC吐̵E"cQ_xnEɉldp[ p )~HX:iiaP4Vm iJ%O|~]4u>z}1 p KRXN@ʝZ%F;Cy&B2oԉi"h=S X!*~B Ҷ3Pk,SMJڃ4jf޽%f>|fJ; W3o0ŠpP~Y2Jҙs1j.)Vq:J: 9`va-PaLiO?B2 <.B'N:w,L2W\1Tj(EAHZ|:}xP}^!.XR]~Gr120#O$ɚI~:xy#<|6Lauꊱ7N'5,;Pw!`31PTAqÊطfJ[ƺH\=?ponC;p%Syp4[&4XS1K'%#W > i4JːhSC ΜYct KEi>$cB]< ` ʹ9%) za8H^ӦI]w`(Ζ̦Ko0ƟFeHCJCt;Z6(a- enm;S9fזVJ`'J2!V-'hSn(T 82V̎;:dSA;RDoS:4d; dK%9Xj(FCXSX(L#0*A04%eYΘЭF*) fzl|#z0z(bT#8 ؃>ceA! 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In ) /S /Span >> endobj 2396 0 obj << /Pg 5585 0 R /P 5473 0 R /K 87 /ActualText (line with these) /S /Span >> endobj 2397 0 obj << /Pg 5585 0 R /P 5473 0 R /K 88 /ActualText ( ) /S /Span >> endobj 2398 0 obj << /Pg 5585 0 R /P 5473 0 R /K 89 /ActualText (observations) /S /Span >> endobj 2399 0 obj << /Pg 5585 0 R /P 5473 0 R /K 90 /ActualText (, we found that antibod) /S /Span >> endobj 2400 0 obj << /Pg 5585 0 R /P 5473 0 R /K 91 /ActualText (ies) /S /Span >> endobj 2401 0 obj << /Pg 5585 0 R /P 5473 0 R /K 92 /ActualText ( ) /S /Span >> endobj 2402 0 obj << /Pg 5585 0 R /P 5473 0 R /K 93 /ActualText (against elastin) /S /Span >> endobj 2403 0 obj << /Pg 5585 0 R /P 5473 0 R /K 94 /ActualText ( ) /S /Span >> endobj 2404 0 obj << /Pg 5585 0 R /P 5473 0 R /K 95 /ActualText (were) /S /Span >> endobj 2405 0 obj << /Pg 5585 0 R /P 5473 0 R /K 96 /ActualText ( ) /S /Span >> endobj 2406 0 obj << /Pg 5585 0 R /P 5473 0 R /K 97 /ActualText (significantly elevated in ) /S /Span >> endobj 2407 0 obj << /Pg 5585 0 R /P 5473 0 R /K 98 /ActualText (COPD patients) /S /Span >> endobj 2408 0 obj << /Pg 5585 0 R /P 5473 0 R /K 99 /ActualText ( ) /S /Span >> endobj 2409 0 obj << /Pg 5585 0 R /P 5473 0 R /K 100 /ActualText (and that T cells from COPD patients bear T cell receptors that recognize elastin ) /S /Span >> endobj 2410 0 obj << /Pg 5585 0 R /P 5473 0 R /K 101 /ActualText (peptides) /S /Span >> endobj 2411 0 obj << /Pg 5585 0 R /P 5473 0 R /K 102 /ActualText (. Moreover, we demonstrate that elastin ) /S /Span >> endobj 2412 0 obj << /Pg 5585 0 R /P 5473 0 R /K 103 /ActualText (s) /S /Span >> endobj 2413 0 obj << /Pg 5585 0 R /P 5473 0 R /K 104 /ActualText (ensitization and challenge induced the ) /S /Span >> endobj 2414 0 obj << /Pg 5585 0 R /P 5473 0 R /K 105 /ActualText (Th1) /S /Span >> endobj 2415 0 obj << /Pg 5585 0 R /P 5473 0 R /K 106 /ActualText (-) /S /Span >> endobj 2416 0 obj << /Pg 5585 0 R /P 5473 0 R /K 107 /ActualText (predominant immune response and subsequent bronchitis) /S /Span >> endobj 2417 0 obj << /Pg 5585 0 R /P 5473 0 R /K 108 /ActualText (-) /S /Span >> endobj 2418 0 obj << /Pg 5585 0 R /P 5473 0 R /K 109 /ActualText (like phenotypes) /S /Span >> endobj 2419 0 obj << /Pg 5585 0 R /P 5473 0 R /K 110 /ActualText (, demonstrati) /S /Span >> endobj 2420 0 obj << /Pg 5585 0 R /P 5473 0 R /K 111 /ActualText (ng that ) /S /Span >> endobj 2421 0 obj << /Pg 5585 0 R /P 5473 0 R /K 112 /ActualText (elastin, as a self) /S /Span >> endobj 2422 0 obj << /Pg 5585 0 R /P 5473 0 R /K 113 /ActualText (-) /S /Span >> endobj 2423 0 obj << /Pg 5585 0 R /P 5473 0 R /K 114 /ActualText (antigen, is able to elicit autoimmune responses in mice. ) /S /Span >> endobj 2424 0 obj << /Pg 5585 0 R /P 5473 0 R /K 115 /ActualText (Finally, ) /S /Span >> endobj 2425 0 obj << /Pg 5585 0 R /P 5473 0 R /K 116 /ActualText (CS) /S /Span >> endobj 2426 0 obj << /Pg 5585 0 R /P 5473 0 R /K 117 /ActualText (-) /S /Span >> endobj 2427 0 obj << /Pg 5585 0 R /P 5473 0 R /K 118 /ActualText (exposed) /S /Span >> endobj 2428 0 obj << /Pg 5585 0 R /P 5473 0 R /K 119 /ActualText ( ) /S /Span >> endobj 2429 0 obj << /Pg 5585 0 R /P 5473 0 R /K 120 /ActualText (mice ) /S /Span >> endobj 2430 0 obj << /Pg 5585 0 R /P 5473 0 R /K 121 /ActualText (were ) /S /Span >> endobj 2431 0 obj << /Pg 5585 0 R /P 5473 0 R /K 122 /ActualText (sensitized to) /S /Span >> endobj 2432 0 obj << /Pg 5585 0 R /P 5473 0 R /K 123 /ActualText ( ) /S /Span >> endobj 2433 0 obj << /Pg 5585 0 R /P 5473 0 R /K 124 /ActualText (elastin, ) /S /Span >> endobj 2434 0 obj << /Pg 5585 0 R /P 5473 0 R /K 125 /ActualText (providing experimental evidence that) /S /Span >> endobj 2435 0 obj << /Pg 5585 0 R /P 5473 0 R /K 126 /ActualText ( ) /S /Span >> endobj 2436 0 obj << /Pg 5585 0 R /P 5473 0 R /K 127 /ActualText (elastin) /S /Span >> endobj 2437 0 obj << /Pg 5585 0 R /P 5473 0 R /K 128 /ActualText (-) /S /Span >> endobj 2438 0 obj << /Pg 5585 0 R /P 5473 0 R /K 129 /ActualText (mediated autoimmunity ) /S /Span >> endobj 2439 0 obj << /Pg 5585 0 R /P 5473 0 R /K 130 /ActualText (may contribute to the) /S /Span >> endobj 2440 0 obj << /Pg 5585 0 R /P 5473 0 R /K 131 /ActualText ( ) /S /Span >> endobj 2441 0 obj << /Pg 5585 0 R /P 5473 0 R /K 132 /ActualText (pathogenesis) /S /Span >> endobj 2442 0 obj << /Pg 5585 0 R /P 5473 0 R /K 133 /ActualText ( ) /S /Span >> endobj 2443 0 obj << /Pg 5585 0 R /P 5473 0 R /K 134 /ActualText (of COPD) /S /Span >> endobj 2444 0 obj << /Pg 5585 0 R /P 5473 0 R /K 135 /ActualText (.) /S /Span >> endobj 2445 0 obj << /Pg 5585 0 R /P 5473 0 R /K 136 /ActualText ( ) /S /Span >> endobj 2446 0 obj << /Pg 5585 0 R /P 5473 0 R /K 137 /ActualText (Gu et al) /S /Span >> endobj 2447 0 obj << /Pg 5585 0 R /P 5473 0 R /K 138 /ActualText ( ) /S /Span >> endobj 2448 0 obj << /Pg 5585 0 R /P 5473 0 R /K 139 /ActualText (have recently demonstrated that ) /S /Span >> endobj 2449 0 obj << /Pg 5585 0 R /P 5473 0 R /K 140 /ActualText (CD4) /S /Span >> endobj 2450 0 obj << /Pg 5585 0 R /P 5473 0 R /K 141 /ActualText (+) /S /Span >> endobj 2451 0 obj << /Pg 5585 0 R /P 5473 0 R /K 142 /ActualText ( ) /S /Span >> endobj 2452 0 obj << /Pg 5585 0 R /P 5473 0 R /K 143 /ActualText (T ) /S /Span >> endobj 2453 0 obj << /Pg 5585 0 R /P 5473 0 R /K 144 /ActualText (cells from CS) /S /Span >> endobj 2454 0 obj << /Pg 5585 0 R /P 5473 0 R /K 145 /ActualText (-) /S /Span >> endobj 2455 0 obj << /Pg 5585 0 R /P 5473 0 R /K 146 /ActualText (exposed mice were reactive to elastin fragment) /S /Span >> endobj 2456 0 obj << /Pg 5585 0 R /P 5473 0 R /K 147 /ActualText (s) /S /Span >> endobj 2457 0 obj << /Pg 5585 0 R /P 5473 0 R /K 148 /ActualText ( ) /S /Span >> endobj 2458 0 obj << /Pg 5585 0 R /P 5473 0 R /K 149 /ActualText (in vitro) /S /Span >> endobj 2459 0 obj << /Pg 5585 0 R /P 5473 0 R /K 150 /ActualText (, and that ) /S /Span >> endobj 2460 0 obj << /Pg 5585 0 R /P 5473 0 R /K 151 /ActualText (mice immunized ) /S /Span >> endobj 2461 0 obj << /Pg 5585 0 R /P 5473 0 R /K 152 /ActualText (with a combination of human ) /S /Span >> endobj 2462 0 obj << /Pg 5585 0 R /P 5473 0 R /K 153 /ActualText (and) /S /Span >> endobj 2463 0 obj << /Pg 5585 0 R /P 5473 0 R /K 154 /ActualText ( ) /S /Span >> endobj 2464 0 obj << /Pg 5585 0 R /P 5473 0 R /K 155 /ActualText (rat ) /S /Span >> endobj 2465 0 obj << /Pg 5585 0 R /P 5473 0 R /K 156 /ActualText (elastin fragments) /S /Span >> endobj 2466 0 obj << /Pg 5585 0 R /P 5473 0 R /K 157 /ActualText ( ) /S /Span >> endobj 2467 0 obj << /Pg 5585 0 R /P 5473 0 R /K 158 /ActualText (showed increased infiltration of innate and ) /S /Span >> endobj 2468 0 obj << /Pg 5585 0 R /P 5473 0 R /K 159 /ActualText (adaptive immune cells to the lungs and developed emphysema) /S /Span >> endobj 2469 0 obj << /Pg 5585 0 R /P 5473 0 R /K 160 /ActualText ( ) /S /Span >> endobj 2470 0 obj << /Pg 5585 0 R /P 5473 0 R /K 161 /ActualText ([18], completely in agreement with ) /S /Span >> endobj 2471 0 obj << /Pg 5585 0 R /P 5473 0 R /K 162 /ActualText (our conclusions.) /S /Span >> endobj 2472 0 obj << /Pg 5585 0 R /P 5473 0 R /K 163 /ActualText ( ) /S /Span >> endobj 2311 0 obj << /Pg 5585 0 R /P 5472 0 R /K 2 /ActualText (The nature of the molecular mechanisms that drive CS) /S /Span >> endobj 2312 0 obj << /Pg 5585 0 R /P 5472 0 R /K 3 /ActualText (-) /S /Span >> endobj 2313 0 obj << /Pg 5585 0 R /P 5472 0 R /K 4 /ActualText (induced airway inflammation remains a ) /S /Span >> endobj 2314 0 obj << /Pg 5585 0 R /P 5472 0 R /K 5 /ActualText (central) /S /Span >> endobj 2315 0 obj << /Pg 5585 0 R /P 5472 0 R /K 6 /ActualText ( ) /S /Span >> endobj 2316 0 obj << /Pg 5585 0 R /P 5472 0 R /K 7 /ActualText (question in COPD research. Building on previous ) /S /Span >> endobj 2317 0 obj << /Pg 5585 0 R /P 5472 0 R /K 8 /ActualText (reports) /S /Span >> endobj 2318 0 obj << /Pg 5585 0 R /P 5472 0 R /K 9 /ActualText ( ) /S /Span >> endobj 2319 0 obj << /Pg 5585 0 R /P 5472 0 R /K 10 /ActualText (that elastin may ) /S /Span >> endobj 2320 0 obj << /Pg 5585 0 R /P 5472 0 R /K 11 /ActualText (drive) /S /Span >> endobj 2321 0 obj << /Pg 5585 0 R /P 5472 0 R /K 12 /ActualText ( ) /S /Span >> endobj 2322 0 obj << /Pg 5585 0 R /P 5472 0 R /K 13 /ActualText (autoimmune ) /S /Span >> endobj 2323 0 obj << /Pg 5585 0 R /P 5472 0 R /K 14 /ActualText (processes) /S /Span >> endobj 2324 0 obj << /Pg 5585 0 R /P 5472 0 R /K 15 /ActualText ( ) /S /Span >> endobj 2325 0 obj << /Pg 5585 0 R /P 5472 0 R /K 16 /ActualText (in COPD) /S /Span >> endobj 2326 0 obj << /Pg 5585 0 R /P 5472 0 R /K 17 /ActualText ( ) /S /Span >> endobj 2327 0 obj << /Pg 5585 0 R /P 5472 0 R /K 18 /ActualText ([) /S /Span >> endobj 2328 0 obj << /Pg 5585 0 R /P 5472 0 R /K 19 /ActualText (6) /S /Span >> endobj 2329 0 obj << /Pg 5585 0 R /P 5472 0 R /K 20 /ActualText (, ) /S /Span >> endobj 2330 0 obj << /Pg 5585 0 R /P 5472 0 R /K 21 /ActualText (7) /S /Span >> endobj 2331 0 obj << /Pg 5585 0 R /P 5472 0 R /K 22 /ActualText (]) /S /Span >> endobj 2332 0 obj << /Pg 5585 0 R /P 5472 0 R /K 23 /ActualText (, we ) /S /Span >> endobj 2333 0 obj << /Pg 5585 0 R /P 5472 0 R /K 24 /ActualText (demonstrate) /S /Span >> endobj 2334 0 obj << /Pg 5585 0 R /P 5472 0 R /K 25 /ActualText ( ) /S /Span >> endobj 2335 0 obj << /Pg 5585 0 R /P 5472 0 R /K 26 /ActualText (that) /S /Span >> endobj 2336 0 obj << /Pg 5585 0 R /P 5472 0 R /K 27 /ActualText (, in mice exposed to cigarette smoke,) /S /Span >> endobj 2337 0 obj << /Pg 5585 0 R /P 5472 0 R /K 28 /ActualText ( ) /S /Span >> endobj 2338 0 obj << /Pg 5585 0 R /P 5472 0 R /K 29 /ActualText (elastin serves as a self) /S /Span >> endobj 2339 0 obj << /Pg 5585 0 R /P 5472 0 R /K 30 /ActualText (-) /S /Span >> endobj 2340 0 obj << /Pg 5585 0 R /P 5472 0 R /K 31 /ActualText (antigen and ) /S /Span >> endobj 2341 0 obj << /Pg 5585 0 R /P 5472 0 R /K 32 /ActualText (drives) /S /Span >> endobj 2342 0 obj << /Pg 5585 0 R /P 5472 0 R /K 33 /ActualText ( ) /S /Span >> endobj 2343 0 obj << /Pg 5585 0 R /P 5472 0 R /K 34 /ActualText (adaptive immune ) /S /Span >> endobj 2344 0 obj << /Pg 5585 0 R /P 5472 0 R /K 35 /ActualText (responses) /S /Span >> endobj 2345 0 obj << /Pg 5585 0 R /P 5472 0 R /K 36 /ActualText ( ) /S /Span >> endobj 2346 0 obj << /Pg 5585 0 R /P 5472 0 R /K 37 /ActualText (that ) /S /Span >> endobj 2347 0 obj << /Pg 5585 0 R /P 5472 0 R /K 38 /ActualText (facilitates sensitization ) /S /Span >> endobj 2348 0 obj << /Pg 5585 0 R /P 5472 0 R /K 39 /ActualText (to subsequent elastin) /S /Span >> endobj 2349 0 obj << /Pg 5585 0 R /P 5472 0 R /K 40 /ActualText (-) /S /Span >> endobj 2350 0 obj << /Pg 5585 0 R /P 5472 0 R /K 41 /ActualText (induced) /S /Span >> endobj 2351 0 obj << /Pg 5585 0 R /P 5472 0 R /K 42 /ActualText ( ) /S /Span >> endobj 2352 0 obj << /Pg 5585 0 R /P 5472 0 R /K 43 /ActualText (COPD) /S /Span >> endobj 2353 0 obj << /Pg 5585 0 R /P 5472 0 R /K 44 /ActualText (-) /S /Span >> endobj 2354 0 obj << /Pg 5585 0 R /P 5472 0 R /K 45 /ActualText (like ) /S /Span >> endobj 2355 0 obj << /Pg 5585 0 R /P 5472 0 R /K 46 /ActualText (pathologies) /S /Span >> endobj 2356 0 obj << /Pg 5585 0 R /P 5472 0 R /K 47 /ActualText (. ) /S /Span >> endobj 2357 0 obj << /Pg 5585 0 R /P 5472 0 R /K 48 /ActualText ( ) /S /Span >> endobj 2309 0 obj << /Pg 5585 0 R /P 5471 0 R /K 0 /ActualText (Discussion ) /S /Span >> endobj 2310 0 obj << /Pg 5585 0 R /P 5471 0 R /K 1 /ActualText ( ) /S /Span >> endobj 2307 0 obj << /Pg 5584 0 R /P 5470 0 R /K 188 /ActualText ( ) /S /Span >> endobj 2308 0 obj << /Pg 5584 0 R /P 5470 0 R /K 189 /ActualText ( ) /S /Span >> endobj 2306 0 obj << /Pg 5584 0 R /P 5469 0 R /K 187 /ActualText ( ) /S /Span >> endobj 2249 0 obj << /Pg 5584 0 R /P 5468 0 R /K 130 /ActualText ( ) /S /Span >> endobj 2250 0 obj << /Pg 5584 0 R /P 5468 0 R /K 131 /ActualText (Finally, we examined elastin) /S /Span >> endobj 2251 0 obj << /Pg 5584 0 R /P 5468 0 R /K 132 /ActualText (-) /S /Span >> endobj 2252 0 obj << /Pg 5584 0 R /P 5468 0 R /K 133 /ActualText (mediated immune responses in COPD patients \() /S /Span >> endobj 2253 0 obj << /Pg 5584 0 R /P 5468 0 R /K 134 /ActualText (Table 1) /S /Span >> endobj 2254 0 obj << /Pg 5584 0 R /P 5468 0 R /K 135 /ActualText (\). In ) /S /Span >> endobj 2255 0 obj << /Pg 5584 0 R /P 5468 0 R /K 136 /ActualText (agreement with previous reports ) /S /Span >> endobj 2256 0 obj << /Pg 5584 0 R /P 5468 0 R /K 137 /ActualText ([) /S /Span >> endobj 2257 0 obj << /Pg 5584 0 R /P 5468 0 R /K 138 /ActualText (6) /S /Span >> endobj 2258 0 obj << /Pg 5584 0 R /P 5468 0 R /K 139 /ActualText (, ) /S /Span >> endobj 2259 0 obj << /Pg 5584 0 R /P 5468 0 R /K 140 /ActualText (7) /S /Span >> endobj 2260 0 obj << /Pg 5584 0 R /P 5468 0 R /K 141 /ActualText (]) /S /Span >> endobj 2261 0 obj << /Pg 5584 0 R /P 5468 0 R /K 142 /ActualText (, the levels of elastin antibod) /S /Span >> endobj 2262 0 obj << /Pg 5584 0 R /P 5468 0 R /K 143 /ActualText (ies) /S /Span >> endobj 2263 0 obj << /Pg 5584 0 R /P 5468 0 R /K 144 /ActualText ( ) /S /Span >> endobj 2264 0 obj << /Pg 5584 0 R /P 5468 0 R /K 145 /ActualText (in plasma were elevated in ) /S /Span >> endobj 2265 0 obj << /Pg 5584 0 R /P 5468 0 R /K 146 /ActualText (COPD patients \() /S /Span >> endobj 2266 0 obj << /Pg 5584 0 R /P 5468 0 R /K 147 /ActualText (Fig. ) /S /Span >> endobj 2267 0 obj << /Pg 5584 0 R /P 5468 0 R /K 148 /ActualText (7) /S /Span >> endobj 2268 0 obj << /Pg 5584 0 R /P 5468 0 R /K 149 /ActualText (a) /S /Span >> endobj 2269 0 obj << /Pg 5584 0 R /P 5468 0 R /K 150 /ActualText (\). ) /S /Span >> endobj 2270 0 obj << /Pg 5584 0 R /P 5468 0 R /K 151 /ActualText (Moreover,) /S /Span >> endobj 2271 0 obj << /Pg 5584 0 R /P 5468 0 R /K 152 /ActualText ( ) /S /Span >> endobj 2272 0 obj << /Pg 5584 0 R /P 5468 0 R /K 153 /ActualText (levels of Th1, Th17, and Tc17 in peripheral blood were ) /S /Span >> endobj 2273 0 obj << /Pg 5584 0 R /P 5468 0 R /K 154 /ActualText (significantly increased in COPD patients relative to healthy controls \() /S /Span >> endobj 2274 0 obj << /Pg 5584 0 R /P 5468 0 R /K 155 /ActualText (Fig. ) /S /Span >> endobj 2275 0 obj << /Pg 5584 0 R /P 5468 0 R /K 156 /ActualText (7) /S /Span >> endobj 2276 0 obj << /Pg 5584 0 R /P 5468 0 R /K 157 /ActualText (b) /S /Span >> endobj 2277 0 obj << /Pg 5584 0 R /P 5468 0 R /K 158 /ActualText (\). In induced ) /S /Span >> endobj 2278 0 obj << /Pg 5584 0 R /P 5468 0 R /K 159 /ActualText (sputum, ) /S /Span >> endobj 2279 0 obj << /Pg 5584 0 R /P 5468 0 R /K 160 /ActualText (protein levels of IL17A were increased \() /S /Span >> endobj 2280 0 obj << /Pg 5584 0 R /P 5468 0 R /K 161 /ActualText (Fig. ) /S /Span >> endobj 2281 0 obj << /Pg 5584 0 R /P 5468 0 R /K 162 /ActualText (7) /S /Span >> endobj 2282 0 obj << /Pg 5584 0 R /P 5468 0 R /K 163 /ActualText (c) /S /Span >> endobj 2283 0 obj << /Pg 5584 0 R /P 5468 0 R /K 164 /ActualText (\). To address whether the pathogenic T cells in ) /S /Span >> endobj 2284 0 obj << /Pg 5584 0 R /P 5468 0 R /K 165 /ActualText (COPD could interact with elastin fragments, we selected an HLA) /S /Span >> endobj 2285 0 obj << /Pg 5584 0 R /P 5468 0 R /K 166 /ActualText (-) /S /Span >> endobj 2286 0 obj << /Pg 5584 0 R /P 5468 0 R /K 167 /ActualText (A*02:01 allele which presents a ) /S /Span >> endobj 2287 0 obj << /Pg 5584 0 R /P 5468 0 R /K 168 /ActualText (relatively high affinity to human elastin peptide GVAPGVGV) /S /Span >> endobj 2288 0 obj << /Pg 5584 0 R /P 5468 0 R /K 169 /ActualText (APGV to synthesize a tetramer. ) /S /Span >> endobj 2289 0 obj << /Pg 5584 0 R /P 5468 0 R /K 170 /ActualText (P) /S /Span >> endobj 2290 0 obj << /Pg 5584 0 R /P 5468 0 R /K 171 /ActualText (eripheral blood mononuclear cells \(PBMCs\) from COPD patients with this allele exhibited ) /S /Span >> endobj 2291 0 obj << /Pg 5584 0 R /P 5468 0 R /K 172 /ActualText (significantly higher positive tetramer staining relative to those from healthy controls with the same ) /S /Span >> endobj 2292 0 obj << /Pg 5584 0 R /P 5468 0 R /K 173 /ActualText (allele \() /S /Span >> endobj 2293 0 obj << /Pg 5584 0 R /P 5468 0 R /K 174 /ActualText (Fig. ) /S /Span >> endobj 2294 0 obj << /Pg 5584 0 R /P 5468 0 R /K 175 /ActualText (7) /S /Span >> endobj 2295 0 obj << /Pg 5584 0 R /P 5468 0 R /K 176 /ActualText (d) /S /Span >> endobj 2296 0 obj << /Pg 5584 0 R /P 5468 0 R /K 177 /ActualText (\).) /S /Span >> endobj 2297 0 obj << /Pg 5584 0 R /P 5468 0 R /K 178 /ActualText ( ) /S /Span >> endobj 2298 0 obj << /Pg 5584 0 R /P 5468 0 R /K 179 /ActualText (Taken together, ) /S /Span >> endobj 2299 0 obj << /Pg 5584 0 R /P 5468 0 R /K 180 /ActualText (these clinical data are in line with our observations in our animal ) /S /Span >> endobj 2300 0 obj << /Pg 5584 0 R /P 5468 0 R /K 181 /ActualText (studies, suggesting that elastin drives IL17A) /S /Span >> endobj 2301 0 obj << /Pg 5584 0 R /P 5468 0 R /K 182 /ActualText (-) /S /Span >> endobj 2302 0 obj << /Pg 5584 0 R /P 5468 0 R /K 183 /ActualText (predominant auto) /S /Span >> endobj 2303 0 obj << /Pg 5584 0 R /P 5468 0 R /K 184 /ActualText (-) /S /Span >> endobj 2304 0 obj << /Pg 5584 0 R /P 5468 0 R /K 185 /ActualText (immune processes in COPD.) /S /Span >> endobj 2305 0 obj << /Pg 5584 0 R /P 5468 0 R /K 186 /ActualText ( ) /S /Span >> endobj 2245 0 obj << /Pg 5584 0 R /P 5467 0 R /K 126 /ActualText (Elastin) /S /Span >> endobj 2246 0 obj << /Pg 5584 0 R /P 5467 0 R /K 127 /ActualText (-) /S /Span >> endobj 2247 0 obj << /Pg 5584 0 R /P 5467 0 R /K 128 /ActualText (mediated autoimmunity is elevated in COPD patients) /S /Span >> endobj 2248 0 obj << /Pg 5584 0 R /P 5467 0 R /K 129 /ActualText ( ) /S /Span >> endobj 2244 0 obj << /Pg 5584 0 R /P 5466 0 R /K 125 /ActualText ( ) /S /Span >> endobj 2119 0 obj << /Pg 5584 0 R /P 5465 0 R /K 0 /ActualText ( ) /S /Span >> endobj 2120 0 obj << /Pg 5584 0 R /P 5465 0 R /K 1 /ActualText (Next, we asked whether ) /S /Span >> endobj 2121 0 obj << /Pg 5584 0 R /P 5465 0 R /K 2 /ActualText (repeated elastin challenge in ) /S /Span >> endobj 2122 0 obj << /Pg 5584 0 R /P 5465 0 R /K 3 /ActualText (CS) /S /Span >> endobj 2123 0 obj << /Pg 5584 0 R /P 5465 0 R /K 4 /ActualText (-) /S /Span >> endobj 2124 0 obj << /Pg 5584 0 R /P 5465 0 R /K 5 /ActualText (exposed mice ) /S /Span >> endobj 2125 0 obj << /Pg 5584 0 R /P 5465 0 R /K 6 /ActualText (would) /S /Span >> endobj 2126 0 obj << /Pg 5584 0 R /P 5465 0 R /K 7 /ActualText ( ) /S /Span >> endobj 2127 0 obj << /Pg 5584 0 R /P 5465 0 R /K 8 /ActualText (elicit) /S /Span >> endobj 2128 0 obj << /Pg 5584 0 R /P 5465 0 R /K 9 /ActualText ( ) /S /Span >> endobj 2129 0 obj << /Pg 5584 0 R /P 5465 0 R /K 10 /ActualText (airway ) /S /Span >> endobj 2130 0 obj << /Pg 5584 0 R /P 5465 0 R /K 11 /ActualText (remodelling and airspace enlargement in mice, mimicking human emphysema. ) /S /Span >> endobj 2131 0 obj << /Pg 5584 0 R /P 5465 0 R /K 12 /ActualText (Mice were exposed ) /S /Span >> endobj 2132 0 obj << /Pg 5584 0 R /P 5465 0 R /K 13 /ActualText (to) /S /Span >> endobj 2133 0 obj << /Pg 5584 0 R /P 5465 0 R /K 14 /ActualText ( ) /S /Span >> endobj 2134 0 obj << /Pg 5584 0 R /P 5465 0 R /K 15 /ActualText (CS) /S /Span >> endobj 2135 0 obj << /Pg 5584 0 R /P 5465 0 R /K 16 /ActualText ( ) /S /Span >> endobj 2136 0 obj << /Pg 5584 0 R /P 5465 0 R /K 17 /ActualText (and then ) /S /Span >> endobj 2137 0 obj << /Pg 5584 0 R /P 5465 0 R /K 18 /ActualText (challenged with elastin every other day for 30 ) /S /Span >> endobj 2138 0 obj << /Pg 5584 0 R /P 5465 0 R /K 19 /ActualText (consecutive ) /S /Span >> endobj 2139 0 obj << /Pg 5584 0 R /P 5465 0 R /K 20 /ActualText (days \() /S /Span >> endobj 2140 0 obj << /Pg 5584 0 R /P 5465 0 R /K 21 /ActualText (Fig. ) /S /Span >> endobj 2141 0 obj << /Pg 5584 0 R /P 5465 0 R /K 22 /ActualText (6) /S /Span >> endobj 2142 0 obj << /Pg 5584 0 R /P 5465 0 R /K 23 /ActualText (a) /S /Span >> endobj 2143 0 obj << /Pg 5584 0 R /P 5465 0 R /K 24 /ActualText (\). ) /S /Span >> endobj 2144 0 obj << /Pg 5584 0 R /P 5465 0 R /K 25 /ActualText (Repeated) /S /Span >> endobj 2145 0 obj << /Pg 5584 0 R /P 5465 0 R /K 26 /ActualText ( ) /S /Span >> endobj 2146 0 obj << /Pg 5584 0 R /P 5465 0 R /K 27 /ActualText (elastin challenge ) /S /Span >> endobj 2147 0 obj << /Pg 5584 0 R /P 5465 0 R /K 28 /ActualText (decreased the ) /S /Span >> endobj 2148 0 obj << /Pg 5584 0 R /P 5465 0 R /K 29 /ActualText (body weight \() /S /Span >> endobj 2149 0 obj << /Pg 5584 0 R /P 5465 0 R /K 30 /ActualText (Fig. 6b) /S /Span >> endobj 2150 0 obj << /Pg 5584 0 R /P 5465 0 R /K 31 /ActualText (\) and ) /S /Span >> endobj 2151 0 obj << /Pg 5584 0 R /P 5465 0 R /K 32 /ActualText (induced a sustained ) /S /Span >> endobj 2152 0 obj << /Pg 5584 0 R /P 5465 0 R /K 33 /ActualText (neutrophilic airway inflammation \() /S /Span >> endobj 2153 0 obj << /Pg 5584 0 R /P 5465 0 R /K 34 /ActualText (Fig. ) /S /Span >> endobj 2154 0 obj << /Pg 5584 0 R /P 5465 0 R /K 35 /ActualText (6c) /S /Span >> endobj 2155 0 obj << /Pg 5584 0 R /P 5465 0 R /K 36 /ActualText (\). ) /S /Span >> endobj 2156 0 obj << /Pg 5584 0 R /P 5465 0 R /K 37 /ActualText (Persistent inflammation was associated with a marked ) /S /Span >> endobj 2157 0 obj << /Pg 5584 0 R /P 5465 0 R /K 38 /ActualText (decline in) /S /Span >> endobj 2158 0 obj << /Pg 5584 0 R /P 5465 0 R /K 39 /ActualText ( ) /S /Span >> endobj 2159 0 obj << /Pg 5584 0 R /P 5465 0 R /K 40 /ActualText (FEV) /S /Span >> endobj 2160 0 obj << /Pg 5584 0 R /P 5465 0 R /K 41 /ActualText (20) /S /Span >> endobj 2161 0 obj << /Pg 5584 0 R /P 5465 0 R /K 42 /ActualText ( ) /S /Span >> endobj 2162 0 obj << /Pg 5584 0 R /P 5465 0 R /K 43 /ActualText (and the FEV) /S /Span >> endobj 2163 0 obj << /Pg 5584 0 R /P 5465 0 R /K 44 /ActualText (20) /S /Span >> endobj 2164 0 obj << /Pg 5584 0 R /P 5465 0 R /K 45 /ActualText (/FVC \() /S /Span >> endobj 2165 0 obj << /Pg 5584 0 R /P 5465 0 R /K 46 /ActualText (Fig. ) /S /Span >> endobj 2166 0 obj << /Pg 5584 0 R /P 5465 0 R /K 47 /ActualText (6d) /S /Span >> endobj 2167 0 obj << /Pg 5584 0 R /P 5465 0 R /K 48 /ActualText (\)) /S /Span >> endobj 2168 0 obj << /Pg 5584 0 R /P 5465 0 R /K 49 /ActualText (, ) /S /Span >> endobj 2169 0 obj << /Pg 5584 0 R /P 5465 0 R /K 50 /ActualText (increased the collagen deposition around airways ) /S /Span >> endobj 2170 0 obj << /Pg 5584 0 R /P 5465 0 R /K 51 /ActualText (\() /S /Span >> endobj 2171 0 obj << /Pg 5584 0 R /P 5465 0 R /K 52 /ActualText (Fig. ) /S /Span >> endobj 2172 0 obj << /Pg 5584 0 R /P 5465 0 R /K 53 /ActualText (6e) /S /Span >> endobj 2173 0 obj << /Pg 5584 0 R /P 5465 0 R /K 54 /ActualText (\) and ) /S /Span >> endobj 2174 0 obj << /Pg 5584 0 R /P 5465 0 R /K 55 /ActualText (evidence) /S /Span >> endobj 2175 0 obj << /Pg 5584 0 R /P 5465 0 R /K 56 /ActualText ( ) /S /Span >> endobj 2176 0 obj << /Pg 5584 0 R /P 5465 0 R /K 57 /ActualText (of) /S /Span >> endobj 2177 0 obj << /Pg 5584 0 R /P 5465 0 R /K 58 /ActualText ( ) /S /Span >> endobj 2178 0 obj << /Pg 5584 0 R /P 5465 0 R /K 59 /ActualText (mucus hyperproduct) /S /Span >> endobj 2179 0 obj << /Pg 5584 0 R /P 5465 0 R /K 60 /ActualText (io) /S /Span >> endobj 2180 0 obj << /Pg 5584 0 R /P 5465 0 R /K 61 /ActualText (n ) /S /Span >> endobj 2181 0 obj << /Pg 5584 0 R /P 5465 0 R /K 62 /ActualText (\() /S /Span >> endobj 2182 0 obj << /Pg 5584 0 R /P 5465 0 R /K 63 /ActualText (Fig. ) /S /Span >> endobj 2183 0 obj << /Pg 5584 0 R /P 5465 0 R /K 64 /ActualText (6f) /S /Span >> endobj 2184 0 obj << /Pg 5584 0 R /P 5465 0 R /K 65 /ActualText (\). ) /S /Span >> endobj 2185 0 obj << /Pg 5584 0 R /P 5465 0 R /K 66 /ActualText (Moreover, repeated) /S /Span >> endobj 2186 0 obj << /Pg 5584 0 R /P 5465 0 R /K 67 /ActualText ( ) /S /Span >> endobj 2187 0 obj << /Pg 5584 0 R /P 5465 0 R /K 68 /ActualText (elastin challenge ) /S /Span >> endobj 2188 0 obj << /Pg 5584 0 R /P 5465 0 R /K 69 /ActualText (led to a) /S /Span >> endobj 2189 0 obj << /Pg 5584 0 R /P 5465 0 R /K 70 /ActualText ( ) /S /Span >> endobj 2190 0 obj << /Pg 5584 0 R /P 5465 0 R /K 71 /ActualText (significant increase in lung airspace, as determined by comparative histologic examination ) /S /Span >> endobj 2191 0 obj << /Pg 5584 0 R /P 5465 0 R /K 72 /ActualText (and mean linear intercept \(MLI\) measurements \() /S /Span >> endobj 2192 0 obj << /Pg 5584 0 R /P 5465 0 R /K 73 /ActualText (Fig. ) /S /Span >> endobj 2193 0 obj << /Pg 5584 0 R /P 5465 0 R /K 74 /ActualText (6g) /S /Span >> endobj 2194 0 obj << /Pg 5584 0 R /P 5465 0 R /K 75 /ActualText (\). ) /S /Span >> endobj 2195 0 obj << /Pg 5584 0 R /P 5465 0 R /K 76 /ActualText (These data suggest that CS exposure and ) /S /Span >> endobj 2196 0 obj << /Pg 5584 0 R /P 5465 0 R /K 77 /ActualText (chronic ) /S /Span >> endobj 2197 0 obj << /Pg 5584 0 R /P 5465 0 R /K 78 /ActualText (elastin challenge induced a COPD) /S /Span >> endobj 2198 0 obj << /Pg 5584 0 R /P 5465 0 R /K 79 /ActualText (-) /S /Span >> endobj 2199 0 obj << /Pg 5584 0 R /P 5465 0 R /K 80 /ActualText (like phenotype) /S /Span >> endobj 2200 0 obj << /Pg 5584 0 R /P 5465 0 R /K 81 /ActualText ( ) /S /Span >> endobj 2201 0 obj << /Pg 5584 0 R /P 5465 0 R /K 82 /ActualText (similar) /S /Span >> endobj 2202 0 obj << /Pg 5584 0 R /P 5465 0 R /K 83 /ActualText ( ) /S /Span >> endobj 2203 0 obj << /Pg 5584 0 R /P 5465 0 R /K 84 /ActualText (to what is observed ) /S /Span >> endobj 2204 0 obj << /Pg 5584 0 R /P 5465 0 R /K 85 /ActualText (in mice) /S /Span >> endobj 2205 0 obj << /Pg 5584 0 R /P 5465 0 R /K 86 /ActualText ( ) /S /Span >> endobj 2206 0 obj << /Pg 5584 0 R /P 5465 0 R /K 87 /ActualText (exposed to ) /S /Span >> endobj 2207 0 obj << /Pg 5584 0 R /P 5465 0 R /K 88 /ActualText (CS for 6 months \() /S /Span >> endobj 2208 0 obj << /Pg 5584 0 R /P 5465 0 R /K 89 /ActualText (Supplementary Fig. ) /S /Span >> endobj 2209 0 obj << /Pg 5584 0 R /P 5465 0 R /K 90 /ActualText (6) /S /Span >> endobj 2210 0 obj << /Pg 5584 0 R /P 5465 0 R /K 91 /ActualText (\). It should be noted that in the chronic CS ) /S /Span >> endobj 2211 0 obj << /Pg 5584 0 R /P 5465 0 R /K 92 /ActualText (model, mic) /S /Span >> endobj 2212 0 obj << /Pg 5584 0 R /P 5465 0 R /K 93 /ActualText (e) /S /Span >> endobj 2213 0 obj << /Pg 5584 0 R /P 5465 0 R /K 94 /ActualText ( ) /S /Span >> endobj 2214 0 obj << /Pg 5584 0 R /P 5465 0 R /K 95 /ActualText (were generally 4 months older than those in the CS) /S /Span >> endobj 2215 0 obj << /Pg 5584 0 R /P 5465 0 R /K 96 /ActualText (-) /S /Span >> endobj 2216 0 obj << /Pg 5584 0 R /P 5465 0 R /K 97 /ActualText (elastin model. T) /S /Span >> endobj 2217 0 obj << /Pg 5584 0 R /P 5465 0 R /K 98 /ActualText (hough the levels ) /S /Span >> endobj 2218 0 obj << /Pg 5584 0 R /P 5465 0 R /K 99 /ActualText (of collagen deposition) /S /Span >> endobj 2219 0 obj << /Pg 5584 0 R /P 5465 0 R /K 100 /ActualText ( ) /S /Span >> endobj 2220 0 obj << /Pg 5584 0 R /P 5465 0 R /K 101 /ActualText (and the airspace enlargement were comparable between ) /S /Span >> endobj 2221 0 obj << /Pg 5584 0 R /P 5465 0 R /K 102 /ActualText (the) /S /Span >> endobj 2222 0 obj << /Pg 5584 0 R /P 5465 0 R /K 103 /ActualText ( ) /S /Span >> endobj 2223 0 obj << /Pg 5584 0 R /P 5465 0 R /K 104 /ActualText (two models, ) /S /Span >> endobj 2224 0 obj << /Pg 5584 0 R /P 5465 0 R /K 105 /ActualText (airway inflammation and mucus production induced by 6 months CS exposure appeared to ) /S /Span >> endobj 2225 0 obj << /Pg 5584 0 R /P 5465 0 R /K 106 /ActualText (be ) /S /Span >> endobj 2226 0 obj << /Pg 5584 0 R /P 5465 0 R /K 107 /ActualText (less ) /S /Span >> endobj 2227 0 obj << /Pg 5584 0 R /P 5465 0 R /K 108 /ActualText (severe compared to) /S /Span >> endobj 2228 0 obj << /Pg 5584 0 R /P 5465 0 R /K 109 /ActualText ( ) /S /Span >> endobj 2229 0 obj << /Pg 5584 0 R /P 5465 0 R /K 110 /ActualText (the CS) /S /Span >> endobj 2230 0 obj << /Pg 5584 0 R /P 5465 0 R /K 111 /ActualText (-) /S /Span >> endobj 2231 0 obj << /Pg 5584 0 R /P 5465 0 R /K 112 /ActualText (elastin model \() /S /Span >> endobj 2232 0 obj << /Pg 5584 0 R /P 5465 0 R /K 113 /ActualText (Supplementary) /S /Span >> endobj 2233 0 obj << /Pg 5584 0 R /P 5465 0 R /K 114 /ActualText ( ) /S /Span >> endobj 2234 0 obj << /Pg 5584 0 R /P 5465 0 R /K 115 /ActualText (Fig. ) /S /Span >> endobj 2235 0 obj << /Pg 5584 0 R /P 5465 0 R /K 116 /ActualText (6) /S /Span >> endobj 2236 0 obj << /Pg 5584 0 R /P 5465 0 R /K 117 /ActualText ( ) /S /Span >> endobj 2237 0 obj << /Pg 5584 0 R /P 5465 0 R /K 118 /ActualText (vs) /S /Span >> endobj 2238 0 obj << /Pg 5584 0 R /P 5465 0 R /K 119 /ActualText ( ) /S /Span >> endobj 2239 0 obj << /Pg 5584 0 R /P 5465 0 R /K 120 /ActualText (Fig. ) /S /Span >> endobj 2240 0 obj << /Pg 5584 0 R /P 5465 0 R /K 121 /ActualText (6) /S /Span >> endobj 2241 0 obj << /Pg 5584 0 R /P 5465 0 R /K 122 /ActualText (\)) /S /Span >> endobj 2242 0 obj << /Pg 5584 0 R /P 5465 0 R /K 123 /ActualText (.) /S /Span >> endobj 2243 0 obj << /Pg 5584 0 R /P 5465 0 R /K 124 /ActualText ( ) /S /Span >> endobj 2112 0 obj << /Pg 5583 0 R /P 5464 0 R /K 191 /ActualText (CS sensitization and sub) /S /Span >> endobj 2113 0 obj << /Pg 5583 0 R /P 5464 0 R /K 192 /ActualText (-) /S /Span >> endobj 2114 0 obj << /Pg 5583 0 R /P 5464 0 R /K 193 /ActualText (chronic elastin challenge induce an emphysema) /S /Span >> endobj 2115 0 obj << /Pg 5583 0 R /P 5464 0 R /K 194 /ActualText (-) /S /Span >> endobj 2116 0 obj << /Pg 5583 0 R /P 5464 0 R /K 195 /ActualText (like phenotype in ) /S /Span >> endobj 2117 0 obj << /Pg 5583 0 R /P 5464 0 R /K 196 /ActualText (mice) /S /Span >> endobj 2118 0 obj << /Pg 5583 0 R /P 5464 0 R /K 197 /ActualText ( ) /S /Span >> endobj 2111 0 obj << /Pg 5583 0 R /P 5463 0 R /K 190 /ActualText ( ) /S /Span >> endobj 2101 0 obj << /Pg 5583 0 R /P 5462 0 R /K 180 /ActualText (These data suggest that the elastin fragments induced by CS ) /S /Span >> endobj 2102 0 obj << /Pg 5583 0 R /P 5462 0 R /K 181 /ActualText (exposure) /S /Span >> endobj 2103 0 obj << /Pg 5583 0 R /P 5462 0 R /K 182 /ActualText (, most likely the GXXPG ) /S /Span >> endobj 2104 0 obj << /Pg 5583 0 R /P 5462 0 R /K 183 /ActualText (and XGXPG motifs, ) /S /Span >> endobj 2105 0 obj << /Pg 5583 0 R /P 5462 0 R /K 184 /ActualText (facilitate sensitization to elastin and ) /S /Span >> endobj 2106 0 obj << /Pg 5583 0 R /P 5462 0 R /K 185 /ActualText (orchestrate inflammatory responses and ) /S /Span >> endobj 2107 0 obj << /Pg 5583 0 R /P 5462 0 R /K 186 /ActualText (mucus hyperproduction in the CS) /S /Span >> endobj 2108 0 obj << /Pg 5583 0 R /P 5462 0 R /K 187 /ActualText (-) /S /Span >> endobj 2109 0 obj << /Pg 5583 0 R /P 5462 0 R /K 188 /ActualText (elastin model.) /S /Span >> endobj 2110 0 obj << /Pg 5583 0 R /P 5462 0 R /K 189 /ActualText ( ) /S /Span >> endobj 2053 0 obj << /Pg 5583 0 R /P 5461 0 R /K 132 /ActualText ( ) /S /Span >> endobj 2054 0 obj << /Pg 5583 0 R /P 5461 0 R /K 133 /ActualText (We next treated mice with BA4 antibody during CS sensitization \() /S /Span >> endobj 2055 0 obj << /Pg 5583 0 R /P 5461 0 R /K 134 /ActualText (Fig. ) /S /Span >> endobj 2056 0 obj << /Pg 5583 0 R /P 5461 0 R /K 135 /ActualText (5) /S /Span >> endobj 2057 0 obj << /Pg 5583 0 R /P 5461 0 R /K 136 /ActualText (f) /S /Span >> endobj 2058 0 obj << /Pg 5583 0 R /P 5461 0 R /K 137 /ActualText (\). This antibody has ) /S /Span >> endobj 2059 0 obj << /Pg 5583 0 R /P 5461 0 R /K 138 /ActualText (been shown to block the activity of endogenous elastin peptides, including the GXXPG and ) /S /Span >> endobj 2060 0 obj << /Pg 5583 0 R /P 5461 0 R /K 139 /ActualText (XGXPG motifs, and reduce the CS) /S /Span >> endobj 2061 0 obj << /Pg 5583 0 R /P 5461 0 R /K 140 /ActualText (-) /S /Span >> endobj 2062 0 obj << /Pg 5583 0 R /P 5461 0 R /K 141 /ActualText (induced airway inflammation in mice ) /S /Span >> endobj 2063 0 obj << /Pg 5583 0 R /P 5461 0 R /K 142 /ActualText ([) /S /Span >> endobj 2064 0 obj << /Pg 5583 0 R /P 5461 0 R /K 143 /ActualText (17) /S /Span >> endobj 2065 0 obj << /Pg 5583 0 R /P 5461 0 R /K 144 /ActualText (]) /S /Span >> endobj 2066 0 obj << /Pg 5583 0 R /P 5461 0 R /K 145 /ActualText (. ) /S /Span >> endobj 2067 0 obj << /Pg 5583 0 R /P 5461 0 R /K 146 /ActualText (N) /S /Span >> endobj 2068 0 obj << /Pg 5583 0 R /P 5461 0 R /K 147 /ActualText (eutrophilic ) /S /Span >> endobj 2069 0 obj << /Pg 5583 0 R /P 5461 0 R /K 148 /ActualText (airway inflammation ) /S /Span >> endobj 2070 0 obj << /Pg 5583 0 R /P 5461 0 R /K 149 /ActualText (following elastin challenge in ) /S /Span >> endobj 2071 0 obj << /Pg 5583 0 R /P 5461 0 R /K 150 /ActualText (CS) /S /Span >> endobj 2072 0 obj << /Pg 5583 0 R /P 5461 0 R /K 151 /ActualText (-) /S /Span >> endobj 2073 0 obj << /Pg 5583 0 R /P 5461 0 R /K 152 /ActualText (exposed mice ) /S /Span >> endobj 2074 0 obj << /Pg 5583 0 R /P 5461 0 R /K 153 /ActualText (was ) /S /Span >> endobj 2075 0 obj << /Pg 5583 0 R /P 5461 0 R /K 154 /ActualText (significantly) /S /Span >> endobj 2076 0 obj << /Pg 5583 0 R /P 5461 0 R /K 155 /ActualText ( ) /S /Span >> endobj 2077 0 obj << /Pg 5583 0 R /P 5461 0 R /K 156 /ActualText (attenuated ) /S /Span >> endobj 2078 0 obj << /Pg 5583 0 R /P 5461 0 R /K 157 /ActualText (by BA4 ) /S /Span >> endobj 2079 0 obj << /Pg 5583 0 R /P 5461 0 R /K 158 /ActualText (antibody ) /S /Span >> endobj 2080 0 obj << /Pg 5583 0 R /P 5461 0 R /K 159 /ActualText (treatment \() /S /Span >> endobj 2081 0 obj << /Pg 5583 0 R /P 5461 0 R /K 160 /ActualText (Fig. ) /S /Span >> endobj 2082 0 obj << /Pg 5583 0 R /P 5461 0 R /K 161 /ActualText (5) /S /Span >> endobj 2083 0 obj << /Pg 5583 0 R /P 5461 0 R /K 162 /ActualText (g) /S /Span >> endobj 2084 0 obj << /Pg 5583 0 R /P 5461 0 R /K 163 /ActualText (-) /S /Span >> endobj 2085 0 obj << /Pg 5583 0 R /P 5461 0 R /K 164 /ActualText (i) /S /Span >> endobj 2086 0 obj << /Pg 5583 0 R /P 5461 0 R /K 165 /ActualText (\). ) /S /Span >> endobj 2087 0 obj << /Pg 5583 0 R /P 5461 0 R /K 166 /ActualText (M) /S /Span >> endobj 2088 0 obj << /Pg 5583 0 R /P 5461 0 R /K 167 /ActualText (ucus hyperproduction induced by CS ) /S /Span >> endobj 2089 0 obj << /Pg 5583 0 R /P 5461 0 R /K 168 /ActualText (exposure) /S /Span >> endobj 2090 0 obj << /Pg 5583 0 R /P 5461 0 R /K 169 /ActualText ( ) /S /Span >> endobj 2091 0 obj << /Pg 5583 0 R /P 5461 0 R /K 170 /ActualText (and ) /S /Span >> endobj 2092 0 obj << /Pg 5583 0 R /P 5461 0 R /K 171 /ActualText (elastin challenge was also markedly ) /S /Span >> endobj 2093 0 obj << /Pg 5583 0 R /P 5461 0 R /K 172 /ActualText (reduced) /S /Span >> endobj 2094 0 obj << /Pg 5583 0 R /P 5461 0 R /K 173 /ActualText ( ) /S /Span >> endobj 2095 0 obj << /Pg 5583 0 R /P 5461 0 R /K 174 /ActualText (by BA4 \() /S /Span >> endobj 2096 0 obj << /Pg 5583 0 R /P 5461 0 R /K 175 /ActualText (Fig. ) /S /Span >> endobj 2097 0 obj << /Pg 5583 0 R /P 5461 0 R /K 176 /ActualText (5) /S /Span >> endobj 2098 0 obj << /Pg 5583 0 R /P 5461 0 R /K 177 /ActualText (j) /S /Span >> endobj 2099 0 obj << /Pg 5583 0 R /P 5461 0 R /K 178 /ActualText (\).) /S /Span >> endobj 2100 0 obj << /Pg 5583 0 R /P 5461 0 R /K 179 /ActualText ( ) /S /Span >> endobj 1993 0 obj << /Pg 5583 0 R /P 5460 0 R /K 72 /ActualText (One of the pathologic mechanisms for MMP12 in COPD is due to the production of elastin ) /S /Span >> endobj 1994 0 obj << /Pg 5583 0 R /P 5460 0 R /K 73 /ActualText (fragments containing GXXPG or XGXPG conformational moti) /S /Span >> endobj 1995 0 obj << /Pg 5583 0 R /P 5460 0 R /K 74 /ActualText (v) /S /Span >> endobj 1996 0 obj << /Pg 5583 0 R /P 5460 0 R /K 75 /ActualText (es) /S /Span >> endobj 1997 0 obj << /Pg 5583 0 R /P 5460 0 R /K 76 /ActualText ( ) /S /Span >> endobj 1998 0 obj << /Pg 5583 0 R /P 5460 0 R /K 77 /ActualText (\(where X is a hydrophobic ) /S /Span >> endobj 1999 0 obj << /Pg 5583 0 R /P 5460 0 R /K 78 /ActualText (amino acid\) ) /S /Span >> endobj 2000 0 obj << /Pg 5583 0 R /P 5460 0 R /K 79 /ActualText (which exert a high monocyte chemotactic activity ) /S /Span >> endobj 2001 0 obj << /Pg 5583 0 R /P 5460 0 R /K 80 /ActualText ([) /S /Span >> endobj 2002 0 obj << /Pg 5583 0 R /P 5460 0 R /K 81 /ActualText (17) /S /Span >> endobj 2003 0 obj << /Pg 5583 0 R /P 5460 0 R /K 82 /ActualText (]) /S /Span >> endobj 2004 0 obj << /Pg 5583 0 R /P 5460 0 R /K 83 /ActualText (. To ) /S /Span >> endobj 2005 0 obj << /Pg 5583 0 R /P 5460 0 R /K 84 /ActualText (investigate) /S /Span >> endobj 2006 0 obj << /Pg 5583 0 R /P 5460 0 R /K 85 /ActualText ( ) /S /Span >> endobj 2007 0 obj << /Pg 5583 0 R /P 5460 0 R /K 86 /ActualText (whether th) /S /Span >> endobj 2008 0 obj << /Pg 5583 0 R /P 5460 0 R /K 87 /ActualText (ese) /S /Span >> endobj 2009 0 obj << /Pg 5583 0 R /P 5460 0 R /K 88 /ActualText ( ) /S /Span >> endobj 2010 0 obj << /Pg 5583 0 R /P 5460 0 R /K 89 /ActualText (moti) /S /Span >> endobj 2011 0 obj << /Pg 5583 0 R /P 5460 0 R /K 90 /ActualText (ves) /S /Span >> endobj 2012 0 obj << /Pg 5583 0 R /P 5460 0 R /K 91 /ActualText ( ) /S /Span >> endobj 2013 0 obj << /Pg 5583 0 R /P 5460 0 R /K 92 /ActualText (contribute to inflammatory processes in the CS) /S /Span >> endobj 2014 0 obj << /Pg 5583 0 R /P 5460 0 R /K 93 /ActualText (-) /S /Span >> endobj 2015 0 obj << /Pg 5583 0 R /P 5460 0 R /K 94 /ActualText (elastin model) /S /Span >> endobj 2016 0 obj << /Pg 5583 0 R /P 5460 0 R /K 95 /ActualText (, CS) /S /Span >> endobj 2017 0 obj << /Pg 5583 0 R /P 5460 0 R /K 96 /ActualText (-) /S /Span >> endobj 2018 0 obj << /Pg 5583 0 R /P 5460 0 R /K 97 /ActualText (exposed) /S /Span >> endobj 2019 0 obj << /Pg 5583 0 R /P 5460 0 R /K 98 /ActualText ( ) /S /Span >> endobj 2020 0 obj << /Pg 5583 0 R /P 5460 0 R /K 99 /ActualText (mice ) /S /Span >> endobj 2021 0 obj << /Pg 5583 0 R /P 5460 0 R /K 100 /ActualText (were ) /S /Span >> endobj 2022 0 obj << /Pg 5583 0 R /P 5460 0 R /K 101 /ActualText (challenged with peptides containing repeating VGVAPG) /S /Span >> endobj 2023 0 obj << /Pg 5583 0 R /P 5460 0 R /K 102 /ActualText ( ) /S /Span >> endobj 2024 0 obj << /Pg 5583 0 R /P 5460 0 R /K 103 /ActualText (sequences) /S /Span >> endobj 2025 0 obj << /Pg 5583 0 R /P 5460 0 R /K 104 /ActualText (, a common) /S /Span >> endobj 2026 0 obj << /Pg 5583 0 R /P 5460 0 R /K 105 /ActualText ( ) /S /Span >> endobj 2027 0 obj << /Pg 5583 0 R /P 5460 0 R /K 106 /ActualText (and) /S /Span >> endobj 2028 0 obj << /Pg 5583 0 R /P 5460 0 R /K 107 /ActualText ( ) /S /Span >> endobj 2029 0 obj << /Pg 5583 0 R /P 5460 0 R /K 108 /ActualText (repeated ) /S /Span >> endobj 2030 0 obj << /Pg 5583 0 R /P 5460 0 R /K 109 /ActualText (motif) /S /Span >> endobj 2031 0 obj << /Pg 5583 0 R /P 5460 0 R /K 110 /ActualText ( ) /S /Span >> endobj 2032 0 obj << /Pg 5583 0 R /P 5460 0 R /K 111 /ActualText (in human elastin. ) /S /Span >> endobj 2033 0 obj << /Pg 5583 0 R /P 5460 0 R /K 112 /ActualText (VGVAPG ) /S /Span >> endobj 2034 0 obj << /Pg 5583 0 R /P 5460 0 R /K 113 /ActualText (peptides ) /S /Span >> endobj 2035 0 obj << /Pg 5583 0 R /P 5460 0 R /K 114 /ActualText (elicited a) /S /Span >> endobj 2036 0 obj << /Pg 5583 0 R /P 5460 0 R /K 115 /ActualText ( ) /S /Span >> endobj 2037 0 obj << /Pg 5583 0 R /P 5460 0 R /K 116 /ActualText (bronchitis) /S /Span >> endobj 2038 0 obj << /Pg 5583 0 R /P 5460 0 R /K 117 /ActualText (-) /S /Span >> endobj 2039 0 obj << /Pg 5583 0 R /P 5460 0 R /K 118 /ActualText (like phenotypes ) /S /Span >> endobj 2040 0 obj << /Pg 5583 0 R /P 5460 0 R /K 119 /ActualText (in CS) /S /Span >> endobj 2041 0 obj << /Pg 5583 0 R /P 5460 0 R /K 120 /ActualText (-) /S /Span >> endobj 2042 0 obj << /Pg 5583 0 R /P 5460 0 R /K 121 /ActualText (exposed) /S /Span >> endobj 2043 0 obj << /Pg 5583 0 R /P 5460 0 R /K 122 /ActualText ( ) /S /Span >> endobj 2044 0 obj << /Pg 5583 0 R /P 5460 0 R /K 123 /ActualText (mice ) /S /Span >> endobj 2045 0 obj << /Pg 5583 0 R /P 5460 0 R /K 124 /ActualText (\() /S /Span >> endobj 2046 0 obj << /Pg 5583 0 R /P 5460 0 R /K 125 /ActualText (Fig. ) /S /Span >> endobj 2047 0 obj << /Pg 5583 0 R /P 5460 0 R /K 126 /ActualText (5) /S /Span >> endobj 2048 0 obj << /Pg 5583 0 R /P 5460 0 R /K 127 /ActualText (a) /S /Span >> endobj 2049 0 obj << /Pg 5583 0 R /P 5460 0 R /K 128 /ActualText (-) /S /Span >> endobj 2050 0 obj << /Pg 5583 0 R /P 5460 0 R /K 129 /ActualText (e) /S /Span >> endobj 2051 0 obj << /Pg 5583 0 R /P 5460 0 R /K 130 /ActualText (\). ) /S /Span >> endobj 2052 0 obj << /Pg 5583 0 R /P 5460 0 R /K 131 /ActualText ( ) /S /Span >> endobj 1988 0 obj << /Pg 5583 0 R /P 5459 0 R /K 67 /ActualText (CS) /S /Span >> endobj 1989 0 obj << /Pg 5583 0 R /P 5459 0 R /K 68 /ActualText (-) /S /Span >> endobj 1990 0 obj << /Pg 5583 0 R /P 5459 0 R /K 69 /ActualText (induced elastin fragments drive the subsequent airway inflammation and mucus ) /S /Span >> endobj 1991 0 obj << /Pg 5583 0 R /P 5459 0 R /K 70 /ActualText (production) /S /Span >> endobj 1992 0 obj << /Pg 5583 0 R /P 5459 0 R /K 71 /ActualText ( ) /S /Span >> endobj 1987 0 obj << /Pg 5583 0 R /P 5458 0 R /K 66 /ActualText ( ) /S /Span >> endobj 1975 0 obj << /Pg 5583 0 R /P 5457 0 R /K 54 /ActualText (These data suggest that induction of ) /S /Span >> endobj 1976 0 obj << /Pg 5583 0 R /P 5457 0 R /K 55 /ActualText (MMP12) /S /Span >> endobj 1977 0 obj << /Pg 5583 0 R /P 5457 0 R /K 56 /ActualText ( ) /S /Span >> endobj 1978 0 obj << /Pg 5583 0 R /P 5457 0 R /K 57 /ActualText (is) /S /Span >> endobj 1979 0 obj << /Pg 5583 0 R /P 5457 0 R /K 58 /ActualText ( ) /S /Span >> endobj 1980 0 obj << /Pg 5583 0 R /P 5457 0 R /K 59 /ActualText (critical for CS) /S /Span >> endobj 1981 0 obj << /Pg 5583 0 R /P 5457 0 R /K 60 /ActualText (-) /S /Span >> endobj 1982 0 obj << /Pg 5583 0 R /P 5457 0 R /K 61 /ActualText (induced ) /S /Span >> endobj 1983 0 obj << /Pg 5583 0 R /P 5457 0 R /K 62 /ActualText (sensitization to elastin, ) /S /Span >> endobj 1984 0 obj << /Pg 5583 0 R /P 5457 0 R /K 63 /ActualText (while neutrophils and neutrophil elastase play a ) /S /Span >> endobj 1985 0 obj << /Pg 5583 0 R /P 5457 0 R /K 64 /ActualText (less important role.) /S /Span >> endobj 1986 0 obj << /Pg 5583 0 R /P 5457 0 R /K 65 /ActualText ( ) /S /Span >> endobj 1931 0 obj << /Pg 5583 0 R /P 5456 0 R /K 10 /ActualText ( ) /S /Span >> endobj 1932 0 obj << /Pg 5583 0 R /P 5456 0 R /K 11 /ActualText ( ) /S /Span >> endobj 1933 0 obj << /Pg 5583 0 R /P 5456 0 R /K 12 /ActualText (To assess the function of MMP) /S /Span >> endobj 1934 0 obj << /Pg 5583 0 R /P 5456 0 R /K 13 /ActualText (-) /S /Span >> endobj 1935 0 obj << /Pg 5583 0 R /P 5456 0 R /K 14 /ActualText (12, we exposed ) /S /Span >> endobj 1936 0 obj << /Pg 5583 0 R /P 5456 0 R /K 15 /ActualText (Mmp12) /S /Span >> endobj 1937 0 obj << /Pg 5583 0 R /P 5456 0 R /K 16 /ActualText (-) /S /Span >> endobj 1938 0 obj << /Pg 5583 0 R /P 5456 0 R /K 17 /ActualText (/) /S /Span >> endobj 1939 0 obj << /Pg 5583 0 R /P 5456 0 R /K 18 /ActualText (-) /S /Span >> endobj 1940 0 obj << /Pg 5583 0 R /P 5456 0 R /K 19 /ActualText ( ) /S /Span >> endobj 1941 0 obj << /Pg 5583 0 R /P 5456 0 R /K 20 /ActualText (mice to cigarette smoke followed by ) /S /Span >> endobj 1942 0 obj << /Pg 5583 0 R /P 5456 0 R /K 21 /ActualText (elastin challenge. In wild type mice, ) /S /Span >> endobj 1943 0 obj << /Pg 5583 0 R /P 5456 0 R /K 22 /ActualText (Mmp12) /S /Span >> endobj 1944 0 obj << /Pg 5583 0 R /P 5456 0 R /K 23 /ActualText ( ) /S /Span >> endobj 1945 0 obj << /Pg 5583 0 R /P 5456 0 R /K 24 /ActualText (mRNA expression ) /S /Span >> endobj 1946 0 obj << /Pg 5583 0 R /P 5456 0 R /K 25 /ActualText (was markedly increased in BALF ) /S /Span >> endobj 1947 0 obj << /Pg 5583 0 R /P 5456 0 R /K 26 /ActualText (cells ) /S /Span >> endobj 1948 0 obj << /Pg 5583 0 R /P 5456 0 R /K 27 /ActualText (of mice exposed ) /S /Span >> endobj 1949 0 obj << /Pg 5583 0 R /P 5456 0 R /K 28 /ActualText (to ) /S /Span >> endobj 1950 0 obj << /Pg 5583 0 R /P 5456 0 R /K 29 /ActualText (CS) /S /Span >> endobj 1951 0 obj << /Pg 5583 0 R /P 5456 0 R /K 30 /ActualText ( ) /S /Span >> endobj 1952 0 obj << /Pg 5583 0 R /P 5456 0 R /K 31 /ActualText (\() /S /Span >> endobj 1953 0 obj << /Pg 5583 0 R /P 5456 0 R /K 32 /ActualText (Fig. ) /S /Span >> endobj 1954 0 obj << /Pg 5583 0 R /P 5456 0 R /K 33 /ActualText (4) /S /Span >> endobj 1955 0 obj << /Pg 5583 0 R /P 5456 0 R /K 34 /ActualText (d) /S /Span >> endobj 1956 0 obj << /Pg 5583 0 R /P 5456 0 R /K 35 /ActualText (\). ) /S /Span >> endobj 1957 0 obj << /Pg 5583 0 R /P 5456 0 R /K 36 /ActualText (Importantly) /S /Span >> endobj 1958 0 obj << /Pg 5583 0 R /P 5456 0 R /K 37 /ActualText (, ) /S /Span >> endobj 1959 0 obj << /Pg 5583 0 R /P 5456 0 R /K 38 /ActualText (Mmp12) /S /Span >> endobj 1960 0 obj << /Pg 5583 0 R /P 5456 0 R /K 39 /ActualText (-) /S /Span >> endobj 1961 0 obj << /Pg 5583 0 R /P 5456 0 R /K 40 /ActualText (/) /S /Span >> endobj 1962 0 obj << /Pg 5583 0 R /P 5456 0 R /K 41 /ActualText (-) /S /Span >> endobj 1963 0 obj << /Pg 5583 0 R /P 5456 0 R /K 42 /ActualText ( ) /S /Span >> endobj 1964 0 obj << /Pg 5583 0 R /P 5456 0 R /K 43 /ActualText (mice displayed markedly reduced ) /S /Span >> endobj 1965 0 obj << /Pg 5583 0 R /P 5456 0 R /K 44 /ActualText (neutrophilic inflammation in BALF) /S /Span >> endobj 1966 0 obj << /Pg 5583 0 R /P 5456 0 R /K 45 /ActualText ( ) /S /Span >> endobj 1967 0 obj << /Pg 5583 0 R /P 5456 0 R /K 46 /ActualText (and airways, decreased production of inflammatory cytokines, ) /S /Span >> endobj 1968 0 obj << /Pg 5583 0 R /P 5456 0 R /K 47 /ActualText (and diminished mucus production \() /S /Span >> endobj 1969 0 obj << /Pg 5583 0 R /P 5456 0 R /K 48 /ActualText (Fig. ) /S /Span >> endobj 1970 0 obj << /Pg 5583 0 R /P 5456 0 R /K 49 /ActualText (4e) /S /Span >> endobj 1971 0 obj << /Pg 5583 0 R /P 5456 0 R /K 50 /ActualText (-) /S /Span >> endobj 1972 0 obj << /Pg 5583 0 R /P 5456 0 R /K 51 /ActualText (h) /S /Span >> endobj 1973 0 obj << /Pg 5583 0 R /P 5456 0 R /K 52 /ActualText (\). ) /S /Span >> endobj 1974 0 obj << /Pg 5583 0 R /P 5456 0 R /K 53 /ActualText ( ) /S /Span >> endobj 1921 0 obj << /Pg 5583 0 R /P 5455 0 R /K 0 /ActualText (less effective ) /S /Span >> endobj 1922 0 obj << /Pg 5583 0 R /P 5455 0 R /K 1 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Interestingly, OVA ) /S /Span >> endobj 1830 0 obj << /Pg 5582 0 R /P 5453 0 R /K 142 /ActualText (failed to induce airway inflammation in CS) /S /Span >> endobj 1831 0 obj << /Pg 5582 0 R /P 5453 0 R /K 143 /ActualText (-) /S /Span >> endobj 1832 0 obj << /Pg 5582 0 R /P 5453 0 R /K 144 /ActualText (exposed mice \() /S /Span >> endobj 1833 0 obj << /Pg 5582 0 R /P 5453 0 R /K 145 /ActualText (Fig. ) /S /Span >> endobj 1834 0 obj << /Pg 5582 0 R /P 5453 0 R /K 146 /ActualText (4) /S /Span >> endobj 1835 0 obj << /Pg 5582 0 R /P 5453 0 R /K 147 /ActualText (b) /S /Span >> endobj 1836 0 obj << /Pg 5582 0 R /P 5453 0 R /K 148 /ActualText (\), suggesting that C) /S /Span >> endobj 1837 0 obj << /Pg 5582 0 R /P 5453 0 R /K 149 /ActualText (S) /S /Span >> endobj 1838 0 obj << /Pg 5582 0 R /P 5453 0 R /K 150 /ActualText (-) /S /Span >> endobj 1839 0 obj << /Pg 5582 0 R /P 5453 0 R /K 151 /ActualText (induced ) /S /Span >> endobj 1840 0 obj << /Pg 5582 0 R /P 5453 0 R /K 152 /ActualText 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endobj 1816 0 obj << /Pg 5582 0 R /P 5452 0 R /K 128 /ActualText (elastin) /S /Span >> endobj 1817 0 obj << /Pg 5582 0 R /P 5452 0 R /K 129 /ActualText ( ) /S /Span >> endobj 1807 0 obj << /Pg 5582 0 R /P 5451 0 R /K 119 /ActualText ( ) /S /Span >> endobj 1796 0 obj << /Pg 5582 0 R /P 5450 0 R /K 108 /ActualText (Taken together, these findings show that ) /S /Span >> endobj 1797 0 obj << /Pg 5582 0 R /P 5450 0 R /K 109 /ActualText (CS) /S /Span >> endobj 1798 0 obj << /Pg 5582 0 R /P 5450 0 R /K 110 /ActualText ( ) /S /Span >> endobj 1799 0 obj << /Pg 5582 0 R /P 5450 0 R /K 111 /ActualText (exposure sensitizes to elastin and that the ) /S /Span >> endobj 1800 0 obj << /Pg 5582 0 R /P 5450 0 R /K 112 /ActualText (inflammatory processes ) /S /Span >> endobj 1801 0 obj << /Pg 5582 0 R /P 5450 0 R /K 113 /ActualText (following exposure to elastin were IL17) /S /Span >> endobj 1802 0 obj << /Pg 5582 0 R /P 5450 0 R /K 114 /ActualText (A) /S /Span >> endobj 1803 0 obj << /Pg 5582 0 R /P 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/Span >> endobj 1743 0 obj << /Pg 5582 0 R /P 5449 0 R /K 55 /ActualText (responses, we ) /S /Span >> endobj 1744 0 obj << /Pg 5582 0 R /P 5449 0 R /K 56 /ActualText (next ) /S /Span >> endobj 1745 0 obj << /Pg 5582 0 R /P 5449 0 R /K 57 /ActualText (investigated) /S /Span >> endobj 1746 0 obj << /Pg 5582 0 R /P 5449 0 R /K 58 /ActualText ( ) /S /Span >> endobj 1747 0 obj << /Pg 5582 0 R /P 5449 0 R /K 59 /ActualText (the ) /S /Span >> endobj 1748 0 obj << /Pg 5582 0 R /P 5449 0 R /K 60 /ActualText (role of ) /S /Span >> endobj 1749 0 obj << /Pg 5582 0 R /P 5449 0 R /K 61 /ActualText (IL17A) /S /Span >> endobj 1750 0 obj << /Pg 5582 0 R /P 5449 0 R /K 62 /ActualText ( ) /S /Span >> endobj 1751 0 obj << /Pg 5582 0 R /P 5449 0 R /K 63 /ActualText (in this model. ) /S /Span >> endobj 1752 0 obj << /Pg 5582 0 R /P 5449 0 R /K 64 /ActualText (A) /S /Span >> endobj 1753 0 obj << /Pg 5582 0 R /P 5449 0 R /K 65 /ActualText (ll the bronchitis) /S /Span >> endobj 1754 0 obj << /Pg 5582 0 R /P 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167 /ActualText ( ) /S /Span >> endobj 1546 0 obj << /Pg 5581 0 R /P 5443 0 R /K 102 /ActualText (C) /S /Span >> endobj 1547 0 obj << /Pg 5581 0 R /P 5443 0 R /K 103 /ActualText (igarette smoke) /S /Span >> endobj 1548 0 obj << /Pg 5581 0 R /P 5443 0 R /K 104 /ActualText (-) /S /Span >> endobj 1549 0 obj << /Pg 5581 0 R /P 5443 0 R /K 105 /ActualText (sensitized T cell immunity ) /S /Span >> endobj 1550 0 obj << /Pg 5581 0 R /P 5443 0 R /K 106 /ActualText (facilitates) /S /Span >> endobj 1551 0 obj << /Pg 5581 0 R /P 5443 0 R /K 107 /ActualText ( ) /S /Span >> endobj 1552 0 obj << /Pg 5581 0 R /P 5443 0 R /K 108 /ActualText (mice ) /S /Span >> endobj 1553 0 obj << /Pg 5581 0 R /P 5443 0 R /K 109 /ActualText (to) /S /Span >> endobj 1554 0 obj << /Pg 5581 0 R /P 5443 0 R /K 110 /ActualText ( ) /S /Span >> endobj 1555 0 obj << /Pg 5581 0 R /P 5443 0 R /K 111 /ActualText (elastin) /S /Span >> endobj 1556 0 obj << /Pg 5581 0 R /P 5443 0 R /K 112 /ActualText (-) /S /Span >> endobj 1557 0 obj 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/P 5441 0 R /K 31 /ActualText (towards this antigen. ) /S /Span >> endobj 1476 0 obj << /Pg 5581 0 R /P 5441 0 R /K 32 /ActualText (To explore whether ) /S /Span >> endobj 1477 0 obj << /Pg 5581 0 R /P 5441 0 R /K 33 /ActualText (mice can be sensitized to elastin, ) /S /Span >> endobj 1478 0 obj << /Pg 5581 0 R /P 5441 0 R /K 34 /ActualText (mice were ) /S /Span >> endobj 1479 0 obj << /Pg 5581 0 R /P 5441 0 R /K 35 /ActualText (injected intraperitoneally) /S /Span >> endobj 1480 0 obj << /Pg 5581 0 R /P 5441 0 R /K 36 /ActualText ( ) /S /Span >> endobj 1481 0 obj << /Pg 5581 0 R /P 5441 0 R /K 37 /ActualText (with ) /S /Span >> endobj 1482 0 obj << /Pg 5581 0 R /P 5441 0 R /K 38 /ActualText (elastin and ) /S /Span >> endobj 1483 0 obj << /Pg 5581 0 R /P 5441 0 R /K 39 /S /Span >> endobj 1484 0 obj << /Pg 5581 0 R /P 5441 0 R /K 40 /ActualText ( ) /S /Span >> endobj 1485 0 obj << /Pg 5581 0 R /P 5441 0 R /K 41 /ActualText (followed by intratracheal challenge with elastin ) /S /Span >> endobj 1486 0 obj << /Pg 5581 0 R /P 5441 0 R /K 42 /S /Span >> endobj 1487 0 obj << /Pg 5581 0 R /P 5441 0 R /K 43 /S /Span >> endobj 1488 0 obj << /Pg 5581 0 R /P 5441 0 R /K 44 /S /Span >> endobj 1489 0 obj << /Pg 5581 0 R /P 5441 0 R /K 45 /S /Span >> endobj 1490 0 obj << /Pg 5581 0 R /P 5441 0 R /K 46 /S /Span >> endobj 1491 0 obj << /Pg 5581 0 R /P 5441 0 R /K 47 /S /Span >> endobj 1492 0 obj << /Pg 5581 0 R /P 5441 0 R /K 48 /S /Span >> endobj 1493 0 obj << /Pg 5581 0 R /P 5441 0 R /K 49 /ActualText (.) /S /Span >> endobj 1494 0 obj << /Pg 5581 0 R /P 5441 0 R /K 50 /ActualText ( ) /S /Span >> endobj 1495 0 obj << /Pg 5581 0 R /P 5441 0 R /K 51 /ActualText (E) /S /Span >> endobj 1496 0 obj << /Pg 5581 0 R /P 5441 0 R /K 52 /ActualText (lastin sensitization and challenge induced ) /S /Span >> endobj 1497 0 obj << /Pg 5581 0 R /P 5441 0 R /K 53 /ActualText (a) /S /Span >> endobj 1498 0 obj << /Pg 5581 0 R /P 5441 0 R /K 54 /ActualText ( ) /S /Span >> endobj 1499 0 obj << /Pg 5581 0 R /P 5441 0 R /K 55 /ActualText (bronchitis) /S /Span >> endobj 1500 0 obj << /Pg 5581 0 R /P 5441 0 R /K 56 /ActualText (-) /S /Span >> endobj 1501 0 obj << /Pg 5581 0 R /P 5441 0 R /K 57 /ActualText (like phenotype ) /S /Span >> endobj 1502 0 obj << /Pg 5581 0 R /P 5441 0 R /K 58 /ActualText (c) /S /Span >> endobj 1503 0 obj << /Pg 5581 0 R /P 5441 0 R /K 59 /ActualText (omparable to the ) /S /Span >> endobj 1504 0 obj << /Pg 5581 0 R /P 5441 0 R /K 60 /ActualText (CS) /S /Span >> endobj 1505 0 obj << /Pg 5581 0 R /P 5441 0 R /K 61 /ActualText (-) /S /Span >> endobj 1506 0 obj << /Pg 5581 0 R /P 5441 0 R /K 62 /ActualText (elastin model ) /S /Span >> endobj 1507 0 obj << /Pg 5581 0 R /P 5441 0 R /K 63 /S /Span >> endobj 1508 0 obj << /Pg 5581 0 R /P 5441 0 R /K 64 /S /Span >> endobj 1509 0 obj << /Pg 5581 0 R /P 5441 0 R /K 65 /S /Span >> endobj 1510 0 obj << /Pg 5581 0 R /P 5441 0 R /K 66 /S /Span >> endobj 1511 0 obj << /Pg 5581 0 R /P 5441 0 R /K 67 /S /Span >> endobj 1512 0 obj << /Pg 5581 0 R /P 5441 0 R /K 68 /S /Span >> endobj 1513 0 obj << /Pg 5581 0 R /P 5441 0 R /K 69 /S /Span >> endobj 1514 0 obj << /Pg 5581 0 R /P 5441 0 R /K 70 /S /Span >> endobj 1515 0 obj << /Pg 5581 0 R /P 5441 0 R /K 71 /S /Span >> endobj 1516 0 obj << /Pg 5581 0 R /P 5441 0 R /K 72 /S /Span >> endobj 1517 0 obj << /Pg 5581 0 R /P 5441 0 R /K 73 /S /Span >> endobj 1518 0 obj << /Pg 5581 0 R /P 5441 0 R /K 74 /ActualText (T cell respons) /S /Span >> endobj 1519 0 obj << /Pg 5581 0 R /P 5441 0 R /K 75 /ActualText (e) /S /Span >> endobj 1520 0 obj << /Pg 5581 0 R /P 5441 0 R /K 76 /ActualText ( ) /S /Span >> endobj 1521 0 obj << /Pg 5581 0 R /P 5441 0 R /K 77 /ActualText (was) /S /Span >> endobj 1522 0 obj << /Pg 5581 0 R /P 5441 0 R /K 78 /ActualText ( ) /S /Span >> endobj 1523 0 obj << /Pg 5581 0 R /P 5441 0 R /K 79 /ActualText (Th1) /S /Span >> endobj 1524 0 obj << /Pg 5581 0 R /P 5441 0 R /K 80 /ActualText (-) /S /Span >> endobj 1525 0 obj << /Pg 5581 0 R /P 5441 0 R /K 81 /ActualText (predominant ) /S /Span >> endobj 1526 0 obj << /Pg 5581 0 R /P 5441 0 R /K 82 /S /Span >> endobj 1527 0 obj << /Pg 5581 0 R /P 5441 0 R /K 83 /S /Span >> endobj 1528 0 obj << /Pg 5581 0 R /P 5441 0 R /K 84 /S /Span >> endobj 1529 0 obj << /Pg 5581 0 R /P 5441 0 R /K 85 /S /Span >> endobj 1530 0 obj << /Pg 5581 0 R /P 5441 0 R /K 86 /S /Span >> endobj 1531 0 obj << /Pg 5581 0 R /P 5441 0 R /K 87 /S /Span >> endobj 1532 0 obj << /Pg 5581 0 R /P 5441 0 R /K 88 /S /Span >> endobj 1533 0 obj << /Pg 5581 0 R /P 5441 0 R /K 89 /S /Span >> endobj 1534 0 obj << /Pg 5581 0 R /P 5441 0 R /K 90 /S /Span >> endobj 1535 0 obj << /Pg 5581 0 R /P 5441 0 R /K 91 /S /Span >> endobj 1536 0 obj << /Pg 5581 0 R /P 5441 0 R /K 92 /S /Span >> endobj 1537 0 obj << /Pg 5581 0 R /P 5441 0 R /K 93 /S /Span >> endobj 1538 0 obj << /Pg 5581 0 R /P 5441 0 R /K 94 /S /Span >> endobj 1539 0 obj << /Pg 5581 0 R /P 5441 0 R /K 95 /S /Span >> endobj 1540 0 obj << /Pg 5581 0 R /P 5441 0 R /K 96 /S /Span >> endobj 1541 0 obj << /Pg 5581 0 R /P 5441 0 R /K 97 /S /Span >> endobj 1542 0 obj << /Pg 5581 0 R /P 5441 0 R /K 98 /S /Span >> endobj 1543 0 obj << /Pg 5581 0 R /P 5441 0 R /K 99 /S /Span >> endobj 1544 0 obj << /Pg 5581 0 R /P 5441 0 R /K 100 /ActualText ( ) /S /Span >> endobj 1457 0 obj << /Pg 5581 0 R /P 5440 0 R /K 13 /ActualText (Sensitization and short) /S /Span >> endobj 1458 0 obj << /Pg 5581 0 R /P 5440 0 R /K 14 /ActualText (-) /S /Span >> endobj 1459 0 obj << /Pg 5581 0 R /P 5440 0 R /K 15 /ActualText (term challenge with mouse elastin induce) /S /Span >> endobj 1460 0 obj << /Pg 5581 0 R /P 5440 0 R /K 16 /ActualText ( ) /S /Span >> endobj 1461 0 obj << /Pg 5581 0 R /P 5440 0 R /K 17 /ActualText (Th1 immune responses) /S /Span >> endobj 1462 0 obj << /Pg 5581 0 R /P 5440 0 R /K 18 /ActualText ( ) /S /Span >> endobj 1463 0 obj << /Pg 5581 0 R /P 5440 0 R /K 19 /ActualText (and ) /S /Span >> endobj 1464 0 obj << /Pg 5581 0 R /P 5440 0 R /K 20 /ActualText (a bronchitis) /S /Span >> endobj 1465 0 obj << /Pg 5581 0 R /P 5440 0 R /K 21 /ActualText (-) /S /Span >> endobj 1466 0 obj << /Pg 5581 0 R /P 5440 0 R /K 22 /ActualText (like airway inflammation in mice.) /S /Span >> endobj 1467 0 obj << /Pg 5581 0 R /P 5440 0 R /K 23 /ActualText ( ) /S /Span >> endobj 1456 0 obj << /Pg 5581 0 R /P 5439 0 R /K 12 /ActualText ( ) /S /Span >> endobj 1444 0 obj << /Pg 5581 0 R /P 5438 0 R /K 0 /ActualText (bronchitis) /S /Span >> endobj 1445 0 obj << /Pg 5581 0 R /P 5438 0 R /K 1 /ActualText (-) /S /Span >> endobj 1446 0 obj << /Pg 5581 0 R /P 5438 0 R /K 2 /ActualText (like phenotype) /S /Span >> endobj 1447 0 obj << /Pg 5581 0 R /P 5438 0 R /K 3 /ActualText ( ) /S /Span >> endobj 1448 0 obj << /Pg 5581 0 R /P 5438 0 R /K 4 /ActualText (that appears more robust than) /S /Span >> endobj 1449 0 obj << /Pg 5581 0 R /P 5438 0 R /K 5 /ActualText ( ) /S /Span >> endobj 1450 0 obj << /Pg 5581 0 R /P 5438 0 R /K 6 /ActualText (chronic ) /S /Span >> endobj 1451 0 obj << /Pg 5581 0 R /P 5438 0 R /K 7 /ActualText (CS exposure) /S /Span >> endobj 1452 0 obj << /Pg 5581 0 R /P 5438 0 R /K 8 /ActualText ( ) /S /Span >> endobj 1453 0 obj << /Pg 5581 0 R /P 5438 0 R /K 9 /ActualText (alone) /S /Span >> endobj 1454 0 obj << /Pg 5581 0 R /P 5438 0 R /K 10 /ActualText (. ) /S /Span >> endobj 1455 0 obj << /Pg 5581 0 R /P 5438 0 R /K 11 /ActualText ( ) /S /Span >> endobj 1412 0 obj << /Pg 5348 0 R /P 5437 0 R /K 146 /ActualText (As a direct) /S /Span >> endobj 1413 0 obj << /Pg 5348 0 R /P 5437 0 R /K 147 /ActualText ( ) /S /Span >> endobj 1414 0 obj << /Pg 5348 0 R /P 5437 0 R /K 148 /ActualText (comparison, ) /S /Span >> endobj 1415 0 obj << /Pg 5348 0 R /P 5437 0 R /K 149 /ActualText (inflammatory parameters in the ) /S /Span >> endobj 1416 0 obj << /Pg 5348 0 R /P 5437 0 R /K 150 /ActualText (CS) /S /Span >> endobj 1417 0 obj << /Pg 5348 0 R /P 5437 0 R /K 151 /ActualText (-) /S /Span >> endobj 1418 0 obj << /Pg 5348 0 R /P 5437 0 R /K 152 /ActualText (elastin model were compared with ) /S /Span >> endobj 1419 0 obj << /Pg 5348 0 R /P 5437 0 R /K 153 /ActualText (the chronic CS exposure. CS exposure for 3 months induced airway ) /S /Span >> endobj 1420 0 obj << /Pg 5348 0 R /P 5437 0 R /K 154 /ActualText (inflammation) /S /Span >> endobj 1421 0 obj << /Pg 5348 0 R /P 5437 0 R /K 155 /ActualText (, while ) /S /Span >> endobj 1422 0 obj << /Pg 5348 0 R /P 5437 0 R /K 156 /ActualText (all ) /S /Span >> endobj 1423 0 obj << /Pg 5348 0 R /P 5437 0 R /K 157 /ActualText (inflammatory ) /S /Span >> endobj 1424 0 obj << /Pg 5348 0 R /P 5437 0 R /K 158 /ActualText (markers were lower than those ) /S /Span >> endobj 1425 0 obj << /Pg 5348 0 R /P 5437 0 R /K 159 /ActualText (observed ) /S /Span >> endobj 1426 0 obj << /Pg 5348 0 R /P 5437 0 R /K 160 /ActualText (in the CS) /S /Span >> endobj 1427 0 obj << /Pg 5348 0 R /P 5437 0 R /K 161 /ActualText (-) /S /Span >> endobj 1428 0 obj << /Pg 5348 0 R /P 5437 0 R /K 162 /ActualText (elastin model) /S /Span >> endobj 1429 0 obj << /Pg 5348 0 R /P 5437 0 R /K 163 /ActualText ( ) /S /Span >> endobj 1430 0 obj << /Pg 5348 0 R /P 5437 0 R /K 164 /ActualText (\() /S /Span >> endobj 1431 0 obj << /Pg 5348 0 R /P 5437 0 R /K 165 /ActualText (Fig. 1b) /S /Span >> endobj 1432 0 obj << /Pg 5348 0 R /P 5437 0 R /K 166 /ActualText (-) /S /Span >> endobj 1433 0 obj << /Pg 5348 0 R /P 5437 0 R /K 167 /ActualText (i) /S /Span >> endobj 1434 0 obj << /Pg 5348 0 R /P 5437 0 R /K 168 /ActualText (\)) /S /Span >> endobj 1435 0 obj << /Pg 5348 0 R /P 5437 0 R /K 169 /ActualText (.) /S /Span >> endobj 1436 0 obj << /Pg 5348 0 R /P 5437 0 R /K 170 /ActualText ( ) /S /Span >> endobj 1437 0 obj << /Pg 5348 0 R /P 5437 0 R /K 171 /ActualText (Collectively, these data demonstrate that CS ) /S /Span >> endobj 1438 0 obj << /Pg 5348 0 R /P 5437 0 R /K 172 /ActualText (exposure) /S /Span >> endobj 1439 0 obj << /Pg 5348 0 R /P 5437 0 R /K 173 /ActualText ( ) /S /Span >> endobj 1440 0 obj << /Pg 5348 0 R /P 5437 0 R /K 174 /ActualText (and elastin challenge ) /S /Span >> endobj 1441 0 obj << /Pg 5348 0 R /P 5437 0 R /K 175 /ActualText (induces) /S /Span >> endobj 1442 0 obj << /Pg 5348 0 R /P 5437 0 R /K 176 /ActualText ( ) /S /Span >> endobj 1443 0 obj << /Pg 5348 0 R /P 5437 0 R /K 177 /ActualText (a ) /S /Span >> endobj 1346 0 obj << /Pg 5348 0 R /P 5436 0 R /K 80 /ActualText ( ) /S /Span >> endobj 1347 0 obj << /Pg 5348 0 R /P 5436 0 R /K 81 /ActualText (Flow cytometric ana) /S /Span >> endobj 1348 0 obj << /Pg 5348 0 R /P 5436 0 R /K 82 /ActualText (lysis of lung T cells ) /S /Span >> endobj 1349 0 obj << /Pg 5348 0 R /P 5436 0 R /K 83 /ActualText (\() /S /Span >> endobj 1350 0 obj << /Pg 5348 0 R /P 5436 0 R /K 84 /ActualText (Supplementary Fig. 2) /S /Span >> endobj 1351 0 obj << /Pg 5348 0 R /P 5436 0 R /K 85 /ActualText (\) ) /S /Span >> endobj 1352 0 obj << /Pg 5348 0 R /P 5436 0 R /K 86 /ActualText (showed that CS exposure ) /S /Span >> endobj 1353 0 obj << /Pg 5348 0 R /P 5436 0 R /K 87 /ActualText (followed by ) /S /Span >> endobj 1354 0 obj << /Pg 5348 0 R /P 5436 0 R /K 88 /ActualText (elastin challenge ) /S /Span >> endobj 1355 0 obj << /Pg 5348 0 R /P 5436 0 R /K 89 /ActualText (induced an IL17A) /S /Span >> endobj 1356 0 obj << /Pg 5348 0 R /P 5436 0 R /K 90 /ActualText (-) /S /Span >> endobj 1357 0 obj << /Pg 5348 0 R /P 5436 0 R /K 91 /ActualText (predominant T cell response) /S /Span >> endobj 1358 0 obj << /Pg 5348 0 R /P 5436 0 R /K 92 /ActualText ( ) /S /Span >> endobj 1359 0 obj << /Pg 5348 0 R /P 5436 0 R /K 93 /ActualText (\() /S /Span >> endobj 1360 0 obj << /Pg 5348 0 R /P 5436 0 R /K 94 /ActualText (Fig. 1e) /S /Span >> endobj 1361 0 obj << /Pg 5348 0 R /P 5436 0 R /K 95 /ActualText (\)) /S /Span >> endobj 1362 0 obj << /Pg 5348 0 R /P 5436 0 R /K 96 /ActualText (, while no ) /S /Span >> endobj 1363 0 obj << /Pg 5348 0 R /P 5436 0 R /K 97 /ActualText (increase was observed in IFN) /S /Span >> endobj 1364 0 obj << /Pg 5348 0 R /P 5436 0 R /K 98 /ActualText (g) /S /Span >> endobj 1365 0 obj << /Pg 5348 0 R /P 5436 0 R /K 99 /ActualText ( ) /S /Span >> endobj 1366 0 obj << /Pg 5348 0 R /P 5436 0 R /K 100 /ActualText (positive T cells. Contrasting these observations, ) /S /Span >> endobj 1367 0 obj << /Pg 5348 0 R /P 5436 0 R /K 101 /ActualText (IFN) /S /Span >> endobj 1368 0 obj << /Pg 5348 0 R /P 5436 0 R /K 102 /ActualText (g) /S /Span >> endobj 1369 0 obj << /Pg 5348 0 R /P 5436 0 R /K 103 /ActualText ( ) /S /Span >> endobj 1370 0 obj << /Pg 5348 0 R /P 5436 0 R /K 104 /ActualText (and IL17A ) /S /Span >> endobj 1371 0 obj << /Pg 5348 0 R /P 5436 0 R /K 105 /ActualText (were both significantly elevated in lung tissues ) /S /Span >> endobj 1372 0 obj << /Pg 5348 0 R /P 5436 0 R /K 106 /ActualText (of) /S /Span >> endobj 1373 0 obj << /Pg 5348 0 R /P 5436 0 R /K 107 /ActualText ( ) /S /Span >> endobj 1374 0 obj << /Pg 5348 0 R /P 5436 0 R /K 108 /ActualText (CS ) /S /Span >> endobj 1375 0 obj << /Pg 5348 0 R /P 5436 0 R /K 109 /ActualText (exposed) /S /Span >> endobj 1376 0 obj << /Pg 5348 0 R /P 5436 0 R /K 110 /ActualText ( ) /S /Span >> endobj 1377 0 obj << /Pg 5348 0 R /P 5436 0 R /K 111 /ActualText (and elastin challenge) /S /Span >> endobj 1378 0 obj << /Pg 5348 0 R /P 5436 0 R /K 112 /ActualText (d mice) /S /Span >> endobj 1379 0 obj << /Pg 5348 0 R /P 5436 0 R /K 113 /ActualText ( ) /S /Span >> endobj 1380 0 obj << /Pg 5348 0 R /P 5436 0 R /K 114 /ActualText (\() /S /Span >> endobj 1381 0 obj << /Pg 5348 0 R /P 5436 0 R /K 115 /ActualText (Fig. ) /S /Span >> endobj 1382 0 obj << /Pg 5348 0 R /P 5436 0 R /K 116 /ActualText (1f) /S /Span >> endobj 1383 0 obj << /Pg 5348 0 R /P 5436 0 R /K 117 /ActualText (\).) /S /Span >> endobj 1384 0 obj << /Pg 5348 0 R /P 5436 0 R /K 118 /ActualText ( ) /S /Span >> endobj 1385 0 obj << /Pg 5348 0 R /P 5436 0 R /K 119 /ActualText (CS ) /S /Span >> endobj 1386 0 obj << /Pg 5348 0 R /P 5436 0 R /K 120 /ActualText (exposure) /S /Span >> endobj 1387 0 obj << /Pg 5348 0 R /P 5436 0 R /K 121 /ActualText ( ) /S /Span >> endobj 1388 0 obj << /Pg 5348 0 R /P 5436 0 R /K 122 /ActualText (and elastin challenge also induced a remarkable mucus hyperproduction, as ) /S /Span >> endobj 1389 0 obj << /Pg 5348 0 R /P 5436 0 R /K 123 /ActualText (evidenced) /S /Span >> endobj 1390 0 obj << /Pg 5348 0 R /P 5436 0 R /K 124 /ActualText ( ) /S /Span >> endobj 1391 0 obj << /Pg 5348 0 R /P 5436 0 R /K 125 /ActualText (by enhanced ) /S /Span >> endobj 1392 0 obj << /Pg 5348 0 R /P 5436 0 R /K 126 /ActualText (Muc5ac) /S /Span >> endobj 1393 0 obj << /Pg 5348 0 R /P 5436 0 R /K 127 /ActualText ( ) /S /Span >> endobj 1394 0 obj << /Pg 5348 0 R /P 5436 0 R /K 128 /S /Span >> endobj 1395 0 obj << /Pg 5348 0 R /P 5436 0 R /K 129 /ActualText (mRNA transcripts, ) /S /Span >> endobj 1396 0 obj << /Pg 5348 0 R /P 5436 0 R /K 130 /ActualText (Periodic acid) /S /Span >> endobj 1397 0 obj << /Pg 5348 0 R /P 5436 0 R /K 131 /ActualText ( ) /S /Span >> endobj 1398 0 obj << /Pg 5348 0 R /P 5436 0 R /K 132 /ActualText (Schiff \() /S /Span >> endobj 1399 0 obj << /Pg 5348 0 R /P 5436 0 R /K 133 /ActualText (PAS) /S /Span >> endobj 1400 0 obj << /Pg 5348 0 R /P 5436 0 R /K 134 /ActualText (\)) /S /Span >> endobj 1401 0 obj << /Pg 5348 0 R /P 5436 0 R /K 135 /ActualText (-) /S /Span >> endobj 1402 0 obj << /Pg 5348 0 R /P 5436 0 R /K 136 /ActualText (positive staining, and MUC5AC expression in ) /S /Span >> endobj 1403 0 obj << /Pg 5348 0 R /P 5436 0 R /K 137 /ActualText (the) /S /Span >> endobj 1404 0 obj << /Pg 5348 0 R /P 5436 0 R /K 138 /ActualText ( ) /S /Span >> endobj 1405 0 obj << /Pg 5348 0 R /P 5436 0 R /K 139 /ActualText (airway epithelium ) /S /Span >> endobj 1406 0 obj << /Pg 5348 0 R /P 5436 0 R /K 140 /ActualText (\() /S /Span >> endobj 1407 0 obj << /Pg 5348 0 R /P 5436 0 R /K 141 /ActualText (Fig. 1g) /S /Span >> endobj 1408 0 obj << /Pg 5348 0 R /P 5436 0 R /K 142 /ActualText (-) /S /Span >> endobj 1409 0 obj << /Pg 5348 0 R /P 5436 0 R /K 143 /ActualText (i) /S /Span >> endobj 1410 0 obj << /Pg 5348 0 R /P 5436 0 R /K 144 /ActualText (\).) /S /Span >> endobj 1411 0 obj << /Pg 5348 0 R /P 5436 0 R /K 145 /ActualText ( ) /S /Span >> endobj 1284 0 obj << /Pg 5348 0 R /P 5435 0 R /K 18 /S /Span >> endobj 1285 0 obj << /Pg 5348 0 R /P 5435 0 R /K 19 /ActualText (mice were ) /S /Span >> endobj 1286 0 obj << /Pg 5348 0 R /P 5435 0 R /K 20 /ActualText (exposed to) /S /Span >> endobj 1287 0 obj << /Pg 5348 0 R /P 5435 0 R /K 21 /ActualText ( ) /S /Span >> endobj 1288 0 obj << /Pg 5348 0 R /P 5435 0 R /K 22 /ActualText (CS for two ) /S /Span >> endobj 1289 0 obj << /Pg 5348 0 R /P 5435 0 R /K 23 /ActualText (weeks followed by two weeks of rest prior to challenge with ) /S /Span >> endobj 1290 0 obj << /Pg 5348 0 R /P 5435 0 R /K 24 /ActualText (elastin ) /S /Span >> endobj 1291 0 obj << /Pg 5348 0 R /P 5435 0 R /K 25 /ActualText (on three consecutive days ) /S /Span >> endobj 1292 0 obj << /Pg 5348 0 R /P 5435 0 R /K 26 /ActualText (\() /S /Span >> endobj 1293 0 obj << /Pg 5348 0 R /P 5435 0 R /K 27 /ActualText (Fig. ) /S /Span >> endobj 1294 0 obj << /Pg 5348 0 R /P 5435 0 R /K 28 /ActualText (1a) /S /Span >> endobj 1295 0 obj << /Pg 5348 0 R /P 5435 0 R /K 29 /ActualText (\). ) /S /Span >> endobj 1296 0 obj << /Pg 5348 0 R /P 5435 0 R /K 30 /ActualText (E) /S /Span >> endobj 1297 0 obj << /Pg 5348 0 R /P 5435 0 R /K 31 /ActualText (lastin challenge ) /S /Span >> endobj 1298 0 obj << /Pg 5348 0 R /P 5435 0 R /K 32 /ActualText (in CS) /S /Span >> endobj 1299 0 obj << /Pg 5348 0 R /P 5435 0 R /K 33 /ActualText (-) /S /Span >> endobj 1300 0 obj << /Pg 5348 0 R /P 5435 0 R /K 34 /ActualText (exposed mice ) /S /Span >> endobj 1301 0 obj << /Pg 5348 0 R /P 5435 0 R /K 35 /ActualText (induced significant neutrophilic airway inflammation, ) /S /Span >> endobj 1302 0 obj << /Pg 5348 0 R /P 5435 0 R /K 36 /ActualText (reaching) /S /Span >> endobj 1303 0 obj << /Pg 5348 0 R /P 5435 0 R /K 37 /ActualText ( ) /S /Span >> endobj 1304 0 obj << /Pg 5348 0 R /P 5435 0 R /K 38 /ActualText (up to 60% ) /S /Span >> endobj 1305 0 obj << /Pg 5348 0 R /P 5435 0 R /K 39 /ActualText (neutrophil) /S /Span >> endobj 1306 0 obj << /Pg 5348 0 R /P 5435 0 R /K 40 /ActualText (s) /S /Span >> endobj 1307 0 obj << /Pg 5348 0 R /P 5435 0 R /K 41 /ActualText ( ) /S /Span >> endobj 1308 0 obj << /Pg 5348 0 R /P 5435 0 R /K 42 /ActualText (in ) /S /Span >> endobj 1309 0 obj << /Pg 5348 0 R /P 5435 0 R /K 43 /ActualText (the ) /S /Span >> endobj 1310 0 obj << /Pg 5348 0 R /P 5435 0 R /K 44 /S /Span >> endobj 1311 0 obj << /Pg 5348 0 R /P 5435 0 R /K 45 /ActualText ( ) /S /Span >> endobj 1312 0 obj << /Pg 5348 0 R /P 5435 0 R /K 46 /ActualText (\() /S /Span >> endobj 1313 0 obj << /Pg 5348 0 R /P 5435 0 R /K 47 /ActualText (Fig. 1b) /S /Span >> endobj 1314 0 obj << /Pg 5348 0 R /P 5435 0 R /K 48 /ActualText (\).) /S /Span >> endobj 1315 0 obj << /Pg 5348 0 R /P 5435 0 R /K 49 /ActualText ( ) /S /Span >> endobj 1316 0 obj << /Pg 5348 0 R /P 5435 0 R /K 50 /ActualText (Histological ) /S /Span >> endobj 1317 0 obj << /Pg 5348 0 R /P 5435 0 R /K 51 /ActualText (assessment showed neutrophilic airway inflammation, corroborating the observation) /S /Span >> endobj 1318 0 obj << /Pg 5348 0 R /P 5435 0 R /K 52 /ActualText (s) /S /Span >> endobj 1319 0 obj << /Pg 5348 0 R /P 5435 0 R /K 53 /ActualText ( ) /S /Span >> endobj 1320 0 obj << /Pg 5348 0 R /P 5435 0 R /K 54 /ActualText (in the BALF ) /S /Span >> endobj 1321 0 obj << /Pg 5348 0 R /P 5435 0 R /K 55 /ActualText (\() /S /Span >> endobj 1322 0 obj << /Pg 5348 0 R /P 5435 0 R /K 56 /ActualText (Fig. 1c\)) /S /Span >> endobj 1323 0 obj << /Pg 5348 0 R /P 5435 0 R /K 57 /ActualText (. ) /S /Span >> endobj 1324 0 obj << /Pg 5348 0 R /P 5435 0 R /K 58 /ActualText (N) /S /Span >> endobj 1325 0 obj << /Pg 5348 0 R /P 5435 0 R /K 59 /ActualText (eutrophilic inflammation was associated with increased expression of IL6, CXCL1, ) /S /Span >> endobj 1326 0 obj << /Pg 5348 0 R /P 5435 0 R /K 60 /ActualText (and CXCL2 in BALF \() /S /Span >> endobj 1327 0 obj << /Pg 5348 0 R /P 5435 0 R /K 61 /ActualText (Fig. 1d) /S /Span >> endobj 1328 0 obj << /Pg 5348 0 R /P 5435 0 R /K 62 /ActualText (\). ) /S /Span >> endobj 1329 0 obj << /Pg 5348 0 R /P 5435 0 R /K 63 /ActualText (Neutrophilic inflammation elicited by elastin challenge in ) /S /Span >> endobj 1330 0 obj << /Pg 5348 0 R /P 5435 0 R /K 64 /ActualText (CS) /S /Span >> endobj 1331 0 obj << /Pg 5348 0 R /P 5435 0 R /K 65 /ActualText (-) /S /Span >> endobj 1332 0 obj << /Pg 5348 0 R /P 5435 0 R /K 66 /ActualText (exposed mice was dose dependent \() /S /Span >> endobj 1333 0 obj << /Pg 5348 0 R /P 5435 0 R /K 67 /ActualText (Supplementary Fig. 1) /S /Span >> endobj 1334 0 obj << /Pg 5348 0 R /P 5435 0 R /K 68 /ActualText (\). While decreased, we observed ) /S /Span >> endobj 1335 0 obj << /Pg 5348 0 R /P 5435 0 R /K 69 /ActualText (significant neutrophilic airway inflammation and increased expression of CXCL1, CXCL2, and ) /S /Span >> endobj 1336 0 obj << /Pg 5348 0 R /P 5435 0 R /K 70 /ActualText (IL) /S /Span >> endobj 1337 0 obj << /Pg 5348 0 R /P 5435 0 R /K 71 /ActualText (-) /S /Span >> endobj 1338 0 obj << /Pg 5348 0 R /P 5435 0 R /K 72 /ActualText (6 ) /S /Span >> endobj 1339 0 obj << /Pg 5348 0 R /P 5435 0 R /K 73 /ActualText (following challenge with lower concentration of elastin. Of note, n) /S /Span >> endobj 1340 0 obj << /Pg 5348 0 R /P 5435 0 R /K 74 /ActualText (o inflammation was ) /S /Span >> endobj 1341 0 obj << /Pg 5348 0 R /P 5435 0 R /K 75 /ActualText (observed in mice exposed to CS and challenged with vehicle \(normal saline\) or mice challenged ) /S /Span >> endobj 1342 0 obj << /Pg 5348 0 R /P 5435 0 R /K 76 /ActualText (with ) /S /Span >> endobj 1343 0 obj << /Pg 5348 0 R /P 5435 0 R /K 77 /S /Span >> endobj 1344 0 obj << /Pg 5348 0 R /P 5435 0 R /K 78 /ActualText ( ) /S /Span >> endobj 1345 0 obj << /Pg 5348 0 R /P 5435 0 R /K 79 /ActualText ( ) /S /Span >> endobj 1268 0 obj << /Pg 5348 0 R /P 5434 0 R /K 2 /ActualText (Exposure to ) /S /Span >> endobj 1269 0 obj << /Pg 5348 0 R /P 5434 0 R /K 3 /ActualText (CS ) /S /Span >> endobj 1270 0 obj << /Pg 5348 0 R /P 5434 0 R /K 4 /ActualText (sensitizes to) /S /Span >> endobj 1271 0 obj << /Pg 5348 0 R /P 5434 0 R /K 5 /ActualText ( ) /S /Span >> endobj 1272 0 obj << /Pg 5348 0 R /P 5434 0 R /K 6 /ActualText (elastin ) /S /Span >> endobj 1273 0 obj << /Pg 5348 0 R /P 5434 0 R /K 7 /ActualText (and elicits) /S /Span >> endobj 1274 0 obj << /Pg 5348 0 R /P 5434 0 R /K 8 /ActualText ( ) /S /Span >> endobj 1275 0 obj << /Pg 5348 0 R /P 5434 0 R /K 9 /ActualText (IL) /S /Span >> endobj 1276 0 obj << /Pg 5348 0 R /P 5434 0 R /K 10 /ActualText (17A) /S /Span >> endobj 1277 0 obj << /Pg 5348 0 R /P 5434 0 R /K 11 /ActualText (-) /S /Span >> endobj 1278 0 obj << /Pg 5348 0 R /P 5434 0 R /K 12 /ActualText (predominant immune responses and ) /S /Span >> endobj 1279 0 obj << /Pg 5348 0 R /P 5434 0 R /K 13 /ActualText (bronchitis) /S /Span >> endobj 1280 0 obj << /Pg 5348 0 R /P 5434 0 R /K 14 /ActualText (-) /S /Span >> endobj 1281 0 obj << /Pg 5348 0 R /P 5434 0 R /K 15 /ActualText (like airway inflammation) /S /Span >> endobj 1282 0 obj << /Pg 5348 0 R /P 5434 0 R /K 16 /ActualText (.) /S /Span >> endobj 1283 0 obj << /Pg 5348 0 R /P 5434 0 R /K 17 /ActualText ( ) /S /Span >> endobj 1266 0 obj << /Pg 5348 0 R /P 5433 0 R /K 0 /ActualText (Results) /S /Span >> endobj 1267 0 obj << /Pg 5348 0 R /P 5433 0 R /K 1 /ActualText ( ) /S /Span >> endobj 948 0 obj << /Pg 5347 0 R /P 5432 0 R /K 23 /ActualText (Data are presented as mean ) /S /Span >> endobj 949 0 obj << /Pg 5347 0 R /P 5432 0 R /K 24 /ActualText () /S /Span >> endobj 950 0 obj << /Pg 5347 0 R /P 5432 0 R /K 25 /ActualText ( ) /S /Span >> endobj 951 0 obj << /Pg 5347 0 R /P 5432 0 R /K 26 /ActualText (SEM) /S /Span >> endobj 952 0 obj << /Pg 5347 0 R /P 5432 0 R /K 27 /ActualText (. BMI: body mass index; FEV1: ) /S /Span >> endobj 953 0 obj << /Pg 5347 0 R /P 5432 0 R /K 28 /ActualText (f) /S /Span >> endobj 954 0 obj << /Pg 5347 0 R /P 5432 0 R /K 29 /ActualText (orced ) /S /Span >> endobj 955 0 obj << /Pg 5347 0 R /P 5432 0 R /K 30 /ActualText (e) /S /Span >> endobj 956 0 obj << /Pg 5347 0 R /P 5432 0 R /K 31 /ActualText (xpiratory ) /S /Span >> endobj 957 0 obj << /Pg 5347 0 R /P 5432 0 R /K 32 /ActualText (v) /S /Span >> endobj 958 0 obj << /Pg 5347 0 R /P 5432 0 R /K 33 /ActualText (olume in ) /S /Span >> endobj 959 0 obj << /Pg 5347 0 R /P 5432 0 R /K 34 /ActualText (the first second; FVC: forced vital capacity; ICS: inhaled corticosteroid; ) /S /Span >> endobj 960 0 obj << /Pg 5347 0 R /P 5432 0 R /K 35 /ActualText (GOLD:) /S /Span >> endobj 961 0 obj << /Pg 5347 0 R /P 5432 0 R /K 36 /ActualText ( ) /S /Span >> endobj 962 0 obj << /Pg 5347 0 R /P 5432 0 R /K 37 /ActualText (g) /S /Span >> endobj 963 0 obj << /Pg 5347 0 R /P 5432 0 R /K 38 /ActualText (lobal ) /S /Span >> endobj 964 0 obj << /Pg 5347 0 R /P 5432 0 R /K 39 /ActualText (i) /S /Span >> endobj 965 0 obj << /Pg 5347 0 R /P 5432 0 R /K 40 /ActualText (nitiative ) /S /Span >> endobj 966 0 obj << /Pg 5347 0 R /P 5432 0 R /K 41 /ActualText (for ) /S /Span >> endobj 967 0 obj << /Pg 5347 0 R /P 5432 0 R /K 42 /ActualText (c) /S /Span >> endobj 968 0 obj << /Pg 5347 0 R /P 5432 0 R /K 43 /ActualText (hronic ) /S /Span >> endobj 969 0 obj << /Pg 5347 0 R /P 5432 0 R /K 44 /ActualText (o) /S /Span >> endobj 970 0 obj << /Pg 5347 0 R /P 5432 0 R /K 45 /ActualText (bstructive ) /S /Span >> endobj 971 0 obj << /Pg 5347 0 R /P 5432 0 R /K 46 /ActualText (l) /S /Span >> endobj 972 0 obj << /Pg 5347 0 R /P 5432 0 R /K 47 /ActualText (ung ) /S /Span >> endobj 973 0 obj << /Pg 5347 0 R /P 5432 0 R /K 48 /ActualText (d) /S /Span >> endobj 974 0 obj << /Pg 5347 0 R /P 5432 0 R /K 49 /ActualText (isease; LABA: long) /S /Span >> endobj 975 0 obj << /Pg 5347 0 R /P 5432 0 R /K 50 /ActualText (-) /S /Span >> endobj 976 0 obj << /Pg 5347 0 R /P 5432 0 R /K 51 /ActualText (acting ) /S /Span >> endobj 977 0 obj << /Pg 5347 0 R /P 5432 0 R /K 52 /ActualText (b) /S /Span >> endobj 978 0 obj << /Pg 5347 0 R /P 5432 0 R /K 53 /ActualText (-) /S /Span >> endobj 979 0 obj << /Pg 5347 0 R /P 5432 0 R /K 54 /ActualText (agonist; LAMA:) /S /Span >> endobj 980 0 obj << /Pg 5347 0 R /P 5432 0 R /K 55 /ActualText ( ) /S /Span >> endobj 981 0 obj << /Pg 5347 0 R /P 5432 0 R /K 56 /ActualText (long) /S /Span >> endobj 982 0 obj << /Pg 5347 0 R /P 5432 0 R /K 57 /ActualText (-) /S /Span >> endobj 983 0 obj << /Pg 5347 0 R /P 5432 0 R /K 58 /ActualText (acting ) /S /Span >> endobj 984 0 obj << /Pg 5347 0 R /P 5432 0 R /K 59 /ActualText (muscarinic agonist; NA: not applicable; SABA: short) /S /Span >> endobj 985 0 obj << /Pg 5347 0 R /P 5432 0 R /K 60 /ActualText (-) /S /Span >> endobj 986 0 obj << /Pg 5347 0 R /P 5432 0 R /K 61 /ActualText (acting ) /S /Span >> endobj 987 0 obj << /Pg 5347 0 R /P 5432 0 R /K 62 /ActualText (b) /S /Span >> endobj 988 0 obj << /Pg 5347 0 R /P 5432 0 R /K 63 /ActualText (2) /S /Span >> endobj 989 0 obj << /Pg 5347 0 R /P 5432 0 R /K 64 /ActualText (-) /S /Span >> endobj 990 0 obj << /Pg 5347 0 R /P 5432 0 R /K 65 /ActualText (a) /S /Span >> endobj 991 0 obj << /Pg 5347 0 R /P 5432 0 R /K 66 /ActualText (gonist. ) /S /Span >> endobj 992 0 obj << /Pg 5347 0 R /P 5432 0 R /K 67 /ActualText ( ) /S /Span >> endobj 993 0 obj << /Pg 5347 0 R /P 5432 0 R /K 68 /ActualText ( ) /S /Span >> endobj 5561 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5618 0 R 5619 0 R 5620 0 R 5621 0 R 1006 0 R] /S /TR >> endobj 5562 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5622 0 R 5623 0 R 5624 0 R 5625 0 R 1023 0 R] /S /TR >> endobj 5563 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5626 0 R 5627 0 R 5628 0 R 5629 0 R] /S /TR >> endobj 5564 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5630 0 R 5631 0 R 5632 0 R 5633 0 R 1051 0 R] /S /TR >> endobj 5565 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5634 0 R 5635 0 R 5636 0 R 5637 0 R] /S /TR >> endobj 5566 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5638 0 R 5639 0 R 5640 0 R 5641 0 R 1079 0 R] /S /TR >> endobj 5567 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5642 0 R 5643 0 R 5644 0 R 5645 0 R] /S /TR >> endobj 5568 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5646 0 R 5647 0 R 5648 0 R 5649 0 R 1108 0 R] /S /TR >> endobj 5569 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5650 0 R 5651 0 R 5652 0 R 5653 0 R] /S /TR >> endobj 5570 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5654 0 R 5655 0 R 5656 0 R 5657 0 R 1135 0 R] /S /TR >> endobj 5571 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5658 0 R 5659 0 R 5660 0 R 5661 0 R] /S /TR >> endobj 5572 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5662 0 R 5663 0 R 5664 0 R 5665 0 R 1158 0 R] /S /TR >> endobj 5573 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5666 0 R 5667 0 R 5668 0 R 5669 0 R] /S /TR >> endobj 5574 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5670 0 R 5671 0 R 5672 0 R 5673 0 R 1185 0 R] /S /TR >> endobj 5575 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5674 0 R 5675 0 R 5676 0 R 5677 0 R] /S /TR >> endobj 5576 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5678 0 R 5679 0 R 5680 0 R 5681 0 R 1211 0 R] /S /TR >> endobj 5577 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5682 0 R 5683 0 R 5684 0 R 5685 0 R] /S /TR >> endobj 5578 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5686 0 R 5687 0 R 5688 0 R 5689 0 R 1238 0 R] /S /TR >> endobj 5579 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5690 0 R 5691 0 R 5692 0 R 5693 0 R] /S /TR >> endobj 5580 0 obj << /Pg 5347 0 R /P 5431 0 R /K [5694 0 R 5695 0 R 5696 0 R 5697 0 R 1265 0 R] /S /TR >> endobj 5694 0 obj << /Pg 5347 0 R /P 5580 0 R /K [1248 0 R 5698 0 R] /S /TD >> endobj 5695 0 obj << /Pg 5347 0 R /P 5580 0 R /K [1253 0 R 5699 0 R] /S /TD >> endobj 5696 0 obj << /Pg 5347 0 R /P 5580 0 R /K [1257 0 R 5700 0 R] /S /TD >> endobj 5697 0 obj << /Pg 5347 0 R /P 5580 0 R /K [1261 0 R 5701 0 R] /S /TD >> endobj 1265 0 obj << /Pg 5347 0 R /P 5580 0 R /K 340 /S /Span >> endobj 1261 0 obj << /Pg 5347 0 R /P 5697 0 R /K 336 /S /Span >> endobj 5701 0 obj << /Pg 5347 0 R /P 5697 0 R /K [1262 0 R 1263 0 R 1264 0 R] /S /P >> endobj 1262 0 obj << /Pg 5347 0 R /P 5701 0 R /K 337 /S /Span >> endobj 1263 0 obj << /Pg 5347 0 R /P 5701 0 R /K 338 /ActualText (NA) /S /Span >> endobj 1264 0 obj << /Pg 5347 0 R /P 5701 0 R /K 339 /ActualText ( ) /S /Span >> endobj 1257 0 obj << /Pg 5347 0 R /P 5696 0 R /K 332 /S /Span >> endobj 5700 0 obj << /Pg 5347 0 R /P 5696 0 R /K [1258 0 R 1259 0 R 1260 0 R] /S /P >> endobj 1258 0 obj << /Pg 5347 0 R /P 5700 0 R /K 333 /S /Span >> endobj 1259 0 obj << /Pg 5347 0 R /P 5700 0 R /K 334 /ActualText (12, \(71\)) /S /Span >> endobj 1260 0 obj << /Pg 5347 0 R /P 5700 0 R /K 335 /ActualText ( ) /S /Span >> endobj 1253 0 obj << /Pg 5347 0 R /P 5695 0 R /K 328 /S /Span >> endobj 5699 0 obj << /Pg 5347 0 R /P 5695 0 R /K [1254 0 R 1255 0 R 1256 0 R] /S /P >> endobj 1254 0 obj << /Pg 5347 0 R /P 5699 0 R /K 329 /S /Span >> endobj 1255 0 obj << /Pg 5347 0 R /P 5699 0 R /K 330 /ActualText (NA) /S /Span >> endobj 1256 0 obj << /Pg 5347 0 R /P 5699 0 R /K 331 /ActualText ( ) /S /Span >> endobj 1248 0 obj << /Pg 5347 0 R /P 5694 0 R /K 323 /S /Span >> endobj 5698 0 obj << /Pg 5347 0 R /P 5694 0 R /K [1249 0 R 1250 0 R 1251 0 R 1252 0 R] /S /P >> endobj 1249 0 obj << /Pg 5347 0 R /P 5698 0 R /K 324 /S /Span >> endobj 1250 0 obj << /Pg 5347 0 R /P 5698 0 R /K 325 /ActualText ( ) /S /Span >> endobj 1251 0 obj << /Pg 5347 0 R /P 5698 0 R /K 326 /ActualText (Theophylline) /S /Span >> endobj 1252 0 obj << /Pg 5347 0 R /P 5698 0 R /K 327 /ActualText ( ) /S /Span >> endobj 5690 0 obj << /Pg 5347 0 R /P 5579 0 R /K 5702 0 R /S /TD >> endobj 5691 0 obj << /Pg 5347 0 R /P 5579 0 R /K 5703 0 R /S /TD >> endobj 5692 0 obj << /Pg 5347 0 R /P 5579 0 R /K 5704 0 R /S /TD >> endobj 5693 0 obj << /Pg 5347 0 R /P 5579 0 R /K 5705 0 R /S /TD >> endobj 5705 0 obj << /Pg 5347 0 R /P 5693 0 R /K [1246 0 R 1247 0 R] /S /P >> endobj 1246 0 obj << /Pg 5347 0 R /P 5705 0 R /K 321 /ActualText (NA) /S 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(Clinical characteristics of healthy controls and hospitalized COPD patients) /S /Span >> endobj 947 0 obj << /Pg 5347 0 R /P 5430 0 R /K 22 /ActualText ( ) /S /Span >> endobj 940 0 obj << /Pg 5347 0 R /P 5429 0 R /K 15 /ActualText ( ) /S /Span >> endobj 925 0 obj << /Pg 5347 0 R /P 5428 0 R /K 0 /ActualText (induced sputum. The diagnosis of COPD was made according to the GOLD \(Global Initiative for ) /S /Span >> endobj 926 0 obj << /Pg 5347 0 R /P 5428 0 R /K 1 /ActualText (Chronic Obstructive Lung Disease\) guidelines) /S /Span >> endobj 927 0 obj << /Pg 5347 0 R /P 5428 0 R /K 2 /ActualText ( ) /S /Span >> endobj 928 0 obj << /Pg 5347 0 R /P 5428 0 R /K 3 /ActualText ([1) /S /Span >> endobj 929 0 obj << /Pg 5347 0 R /P 5428 0 R /K 4 /ActualText (2) /S /Span >> endobj 930 0 obj << /Pg 5347 0 R /P 5428 0 R /K 5 /ActualText (]) /S /Span >> endobj 931 0 obj << /Pg 5347 0 R /P 5428 0 R /K 6 /ActualText (. Exclusion criteria were as follows: any ) /S /Span >> endobj 932 0 obj << /Pg 5347 0 R /P 5428 0 R /K 7 /ActualText (chronic cardiopulmonary disease other than COPD and an inability to give written informed ) /S /Span >> endobj 933 0 obj << /Pg 5347 0 R /P 5428 0 R /K 8 /ActualText (consent or cooperate with the stud) /S /Span >> endobj 934 0 obj << /Pg 5347 0 R /P 5428 0 R /K 9 /ActualText (y investigators. Clinical characteristics are described in Table 1. ) /S /Span >> endobj 935 0 obj << /Pg 5347 0 R /P 5428 0 R /K 10 /ActualText (The study subjects were recruited from two medical centers. The study protocols were approved ) /S /Span >> endobj 936 0 obj << /Pg 5347 0 R /P 5428 0 R /K 11 /ActualText (by the Ethics of Research Committee of the Second Affiliated Hospital, Zhejiang University. ) /S /Span >> endobj 937 0 obj << /Pg 5347 0 R /P 5428 0 R /K 12 /ActualText (Wr) /S /Span >> endobj 938 0 obj << /Pg 5347 0 R /P 5428 0 R /K 13 /ActualText (itten informed consent was obtained from all participants.) /S /Span >> endobj 939 0 obj << /Pg 5347 0 R /P 5428 0 R /K 14 /ActualText ( ) /S /Span >> endobj 922 0 obj << /Pg 5346 0 R /P 5427 0 R /K 189 /ActualText (We enrolled healthy ) /S /Span >> endobj 923 0 obj << /Pg 5346 0 R /P 5427 0 R /K 190 /ActualText (control) /S /Span >> endobj 924 0 obj << /Pg 5346 0 R /P 5427 0 R /K 191 /ActualText (s and hospitalized COPD patients for collecting peripheral blood and ) /S /Span >> endobj 920 0 obj << /Pg 5346 0 R /P 5426 0 R /K 187 /ActualText (Human study subjects) /S /Span >> endobj 921 0 obj << /Pg 5346 0 R /P 5426 0 R /K 188 /ActualText ( ) /S /Span >> endobj 919 0 obj << /Pg 5346 0 R /P 5425 0 R /K 186 /ActualText ( ) /S /Span >> endobj 894 0 obj << /Pg 5346 0 R /P 5424 0 R /K 161 /ActualText (L) /S /Span >> endobj 895 0 obj << /Pg 5346 0 R /P 5424 0 R /K 162 /ActualText (ipopolysaccharide ) /S /Span >> endobj 896 0 obj << /Pg 5346 0 R /P 5424 0 R /K 163 /ActualText (\(LPS: L2630, Sigma) /S /Span >> endobj 897 0 obj << /Pg 5346 0 R /P 5424 0 R /K 164 /ActualText (-) /S /Span >> endobj 898 0 obj << /Pg 5346 0 R /P 5424 0 R /K 165 /ActualText (Aldrich) /S /Span >> endobj 899 0 obj << /Pg 5346 0 R /P 5424 0 R /K 166 /ActualText (, 1 ) /S /Span >> endobj 900 0 obj << /Pg 5346 0 R /P 5424 0 R /K 167 /ActualText (m) /S /Span >> endobj 901 0 obj << /Pg 5346 0 R /P 5424 0 R /K 168 /ActualText (g in) /S /Span >> endobj 902 0 obj << /Pg 5346 0 R /P 5424 0 R /K 169 /ActualText ( ) /S /Span >> endobj 903 0 obj << /Pg 5346 0 R /P 5424 0 R /K 170 /ActualText (50) /S /Span >> endobj 904 0 obj << /Pg 5346 0 R /P 5424 0 R /K 171 /ActualText ( ) /S /Span >> endobj 905 0 obj << /Pg 5346 0 R /P 5424 0 R /K 172 /ActualText (m) /S /Span >> endobj 906 0 obj << /Pg 5346 0 R /P 5424 0 R /K 173 /ActualText (l saline) /S /Span >> endobj 907 0 obj << /Pg 5346 0 R /P 5424 0 R /K 174 /ActualText (\) ) /S /Span >> endobj 908 0 obj << /Pg 5346 0 R /P 5424 0 R /K 175 /ActualText (was) /S /Span >> endobj 909 0 obj << /Pg 5346 0 R /P 5424 0 R /K 176 /ActualText ( ) /S /Span >> endobj 910 0 obj << /Pg 5346 0 R /P 5424 0 R /K 177 /ActualText (delivered ) /S /Span >> endobj 911 0 obj << /Pg 5346 0 R /P 5424 0 R /K 178 /ActualText (intratracheally) /S /Span >> endobj 912 0 obj << /Pg 5346 0 R /P 5424 0 R /K 179 /ActualText ( ) /S /Span >> endobj 913 0 obj << /Pg 5346 0 R /P 5424 0 R /K 180 /ActualText (using a) /S /Span >> endobj 914 0 obj << /Pg 5346 0 R /P 5424 0 R /K 181 /ActualText ( ) /S /Span >> endobj 915 0 obj << /Pg 5346 0 R /P 5424 0 R /K 182 /ActualText (micro) /S /Span >> endobj 916 0 obj << /Pg 5346 0 R /P 5424 0 R /K 183 /ActualText (-) /S /Span >> endobj 917 0 obj << /Pg 5346 0 R /P 5424 0 R /K 184 /ActualText (syringe. ) /S /Span >> endobj 918 0 obj << /Pg 5346 0 R /P 5424 0 R /K 185 /ActualText ( ) /S /Span >> endobj 827 0 obj << /Pg 5346 0 R /P 5423 0 R /K 94 /ActualText (Mouse elastin \(E6402) /S /Span >> endobj 828 0 obj << /Pg 5346 0 R /P 5423 0 R /K 95 /ActualText (-) /S /Span >> endobj 829 0 obj << /Pg 5346 0 R /P 5423 0 R /K 96 /ActualText (SPEC\) was purchased from Sigma) /S /Span >> endobj 830 0 obj << /Pg 5346 0 R /P 5423 0 R /K 97 /ActualText (-) /S /Span >> endobj 831 0 obj << /Pg 5346 0 R /P 5423 0 R /K 98 /ActualText (Aldrich. ) /S /Span >> endobj 832 0 obj << /Pg 5346 0 R /P 5423 0 R /K 99 /ActualText (2 mg/ml e) /S /Span >> endobj 833 0 obj << /Pg 5346 0 R /P 5423 0 R /K 100 /ActualText (lastin was ) /S /Span >> endobj 834 0 obj << /Pg 5346 0 R /P 5423 0 R /K 101 /ActualText (suspended ) /S /Span >> endobj 835 0 obj << /Pg 5346 0 R /P 5423 0 R /K 102 /ActualText (in sterile saline ) /S /Span >> endobj 836 0 obj << /Pg 5346 0 R /P 5423 0 R /K 103 /ActualText (and sonicated) /S /Span >> endobj 837 0 obj << /Pg 5346 0 R /P 5423 0 R /K 104 /ActualText (. 100 ) /S /Span >> endobj 838 0 obj << /Pg 5346 0 R /P 5423 0 R /K 105 /ActualText (m) /S /Span >> endobj 839 0 obj << /Pg 5346 0 R /P 5423 0 R /K 106 /ActualText (g elastin in 50 ) /S /Span >> endobj 840 0 obj << /Pg 5346 0 R /P 5423 0 R /K 107 /ActualText (m) /S /Span >> endobj 841 0 obj << /Pg 5346 0 R /P 5423 0 R /K 108 /ActualText (l) /S /Span >> endobj 842 0 obj << /Pg 5346 0 R /P 5423 0 R /K 109 /ActualText ( ) /S /Span >> endobj 843 0 obj << /Pg 5346 0 R /P 5423 0 R /K 110 /ActualText (saline ) /S /Span >> endobj 844 0 obj << /Pg 5346 0 R /P 5423 0 R /K 111 /ActualText (was administered ) /S /Span >> endobj 845 0 obj << /Pg 5346 0 R /P 5423 0 R /K 112 /ActualText (intratracheally) /S /Span >> endobj 846 0 obj << /Pg 5346 0 R /P 5423 0 R /K 113 /ActualText (. 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Ow--vla- th߾XDSUmrWS&#Ho)ϸh$#BHaLRS5td #{x"elITR\d9&Z$:r[#]@LRE5g5yAf ݷqXHb^7k̤J5DVauZv/s ]tkF8a,kq`n@y$ȝ|a'k ClpZ%|9tvXK@;w*-7{~i4'q$BCjEnVXzd 5Sa9WQۍ% h쥑k,C"΍t?z~lJ[- LLx=ȍ-:sƜ@Xv!gQmiϺs:!;&߰t] g^'HDi%T]cvsU]&F׸D5۸{IDHqRI6Ʒ>ky-tʆ[J}zwۼlZd neOOaĢ7 @@837?"t2Hu7يì|[a9v=0n'Ǫu[lAA:Å+*w g{}h׺@wlh;f۬Hk Z+k杺dl>$>j5hGCMV/,^':T0C?HAk״w,9 o2 VeX.(ZeD"KCE`z}i?DwxF !ڂ֞I;k ɰxKDwB"߫ &ZgHxI8pgHF˙Ay ,DZ@;ɖt {jPǹv#n}WWCkA'^]K(z5:wL~H8d6A zcտmǘkyT(ƳcOpw9Kͳ"Tk4gDk!"KxKn1]N9J5:5 ʕ_Aɀ^͹k}pFƞ/SX>̊XS=jw+ @wrڃlֽw>三 }\kw{\d)>I>SKyNkGMY&7G@QA+t1}lC\8IjFѵI|HMRk *y&hn;^Vd-;~cq|鏄Qr i{ {m:1սϴ~q6M6 /L`jeb'HQl1 ǂ7kku#+UV5woZp>!6V5ϯc=t 'hw1[ R"@PZZЏPP.0A52ֽ!'N쀓wYaupY~?Y'Oic5/$%ݣgVd]UcoG6"f$XI˪mNË#st UZ[Ʃ\ q="uTlh.qH$GPIӕe!&I=Ӷ9|xCcߏyOǔ4n{$A;} %j_*!'*M^+'oЖHC̸S7k+spk9>1:H ŦgIX~095j<ҍ@)"6~˚g|h|ҒZW1js1'ݠNuh`67<1kt {^#/RJuYp l S5恦&36O>KDLVn&[]k Ɨ5_1mQs=25#گRI:?GׇO2HiC}fh4ԗ ! endstream endobj 5904 0 obj << /ColorSpace 5305 0 R /Subtype /Image /Filter /DCTDecode /Length 2853 /Width 118 /Type /XObject /BitsPerComponent 8 /Height 87 >> stream Adobed     $$''$$53335;;;;;;;;;;  %% ## ((%%((22022;;;;;;;;;;Wv"?   3!1AQa"q2B#$Rb34rC%Scs5&DTdE£t6UeuF'Vfv7GWgw5!1AQaq"2B#R3$brCScs4%&5DTdEU6teuFVfv'7GWgw ?w7=C& v4i7[',-t87#M7IH Z  k( 'HNw=>jA{- {}`|RL "<:sjF5ȵsO?$+-=JJ\9ew1eMsIav"`O:{r94'%[]ƺ=ĝv.5Un}ֳ%uu@ + gu`lIy.Zֱ|zzt?F;};VhJފ냅v7 6-V:8\nB! eL_C)ThwD:|Z NhIPuq*C~sxf0 .aed8@DzLff$pbvNJJc,p qw5k8M%ӫK"g(cmc^&yIbhO#T*fXCeW3 caa~j"":ϳ6C>ϟt 63Hx;`u{L"{LO2;@2 :f:G>#OoL͖c}~Gp?ڀԪ:z0JkHpWc՜mn݇Lⴀ@$|~j3onI#蹢(y_k5zu^ݶpxPP'Z,>旇S|۴ {ƻ(Y6z7ˈDY$}0!H\EQшsM%w<$$^͡ۇ?D}~pׅ'&GkH÷ įA506cc dŵcQ$ >C~ mq܎({$ ٻƂ %|Syva>(쇇71y PsJ:ۏeF=bCC^1B^P-p;3+[?/) :)[Mc`IaVw-hieOV́RkwX?*s{ ’\XLo5PτrpЈkOyP:IN6q3--G h(;qZie5Ʃ DGNqJGDTݫczqȐy|Q.`\;'I)v@5֗>kL4~罯5){>IxR]E=hŀ;ī `6\\$j{yua~\ ?kik$ k/ǸÜ\~e$v؃]vt@cٙ~sYkkFڝt wWNEs^v4ґHx5=ve|8kl,K61Ϲ c/g\Mvaן R6ILr'[]eNG-?ސ.LIoˮ֞\>59ۈJbö"$7C,vlݽZ NIC{A|8슺25C.ԓ ! 43Q?$R }"9(봟ϙI7AY{k'>jw:#dZw1%ָոPnj{xuǗb+~X*a:%x 'LI+ R˺~{kMb2Eb4cgAWI˼g'DPeYgs}6CA1vܦ,#NC^?F@ku2E=wd<2בdגq%>^l<&D wG/{Zu@ULd{cx#Dc2t wxϒ1=FԔ9\Ce%vɎDDI)m.ip5<[m DKWKoG׻&_-F{oXCA"|e IѫdƯ]qEݐAqk#|ʒt]-~1.o!D$E`=̩&CoudRI!||Ȓ'uߢ:+6[^f]v `vΥ>j8y.i-_>O Kk1~הݵhs[귓 rI%? endstream endobj 5903 0 obj << /DecodeParms << /Columns 112 /K -1 >> /ImageMask true /Width 112 /BitsPerComponent 1 /Height 1122 /Filter /CCITTFaxDecode /Subtype /Image /Length 451 /Type /XObject >> stream *ƿU@QA&t&7I&Ҵu[mmmXka+ VVZ C gp GV‚ajja: Dk A&NoO[_`]<.B:Q>/ (  P@A( P@ (AA P@A ( P@ `V G_ʠ-"M*TRT-WoxvA5}øuTAҥTR-WU{RwwpX3_@ endstream endobj 5902 0 obj << /Subtype /Image /Filter /FlateDecode /Length 180 /Width 32 /Type /XObject /BitsPerComponent 1 /Height 1122 /ImageMask true >> stream h1 E20rz3Dq?D()~ғ1 0 0 QP|2Mz~w76'R%S=\SjQ]W,z}[#fGNtE P=-˂riKZ냣(AS -s^`zq endstream endobj 5796 0 obj << /Filter /FlateDecode /Length 16401 >> stream h{Ko$^" haqa`xaxa%6|߿AfeIf=TcRe ?zVCFOA?}}?{J8aK=7h'߾}R /T3$ÿ3I?iT9;E%Ŗ6~RH>^e/0YLJX?N ÿall s7(|}8r>i_a˱7aak%|<װ0a>UJ4_]h44SAM"JK -lhqGqn{sA]5< ܈  (Q[]=Xx jKҟU:' 2ep56Ci6w!ٮzIw5lImm?#!cqSq'hnz|{!8VhwB<ҮQm==R|O&Bgp%J=Q7h?`GAc3=m^]6O@jtH:Vcsg[ЌuZ#:GY'ڜn(hŝ9˻ A8X#un~y8f S$KKma#.:$ 37He}Aw/)uR-m@m8@;O;@q#s1zguN!l5;Wwupu-lP|=X-0p$ϓ#dC:FΩh<! og5%:ʻKj&#jE0 ?·AT#(⻿js(5܍L֋Q;:猝HTR aw^g32kpKVPQM՘!+&ZqTџ?+ef59/_~W릟ULM[% km5RJv UHsT !Y Y ~RI|*U*t_ϊ"NXx~'?dND([P~1(¿)Sb͘% 2|,S!6ĩ@KmSzϔ 89$ln uBYznQ*մ< j;:눏O?V9y mB=~FdL|5DtO՘:[^CVΏ=2N n"w$U4)|jJE6ԃD61m-|_(FS HY.]H:{ yްt+y\U!#f(2TuROx}[̢|}xKbQυV&=)Za: 6'=i;*/.G*+K35:ֻUXSxav]:'-t92zdP PmP )yNGF9l3kUd!_t?pݑ*a1ԊILN@ua%qoT EOW'!cOԷoM+gDdUErZHË 9^;CW{搠u\P4Zg ^wn>ٰ5^q1 K V>N ZXA{Vr41A?%8nT͍P"9\F陊 r3L A֐3+>KP,P DZ|Ptip @h:\`J Lx̜]MITQ. 6 ^ gn.Y&Za^Xs$rյH8`z&aa[ >,ßlDJ 3)1M= jnҴ~0s5.<-.zB&jgR $ SHDN'975`;L`+MV

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