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[(st)17.7(ai)18.4(ni)18.9(ng)-434(fo)19.6(r)-440.5(c)0(a)20.4(r)0(c)13.5(i)0(n)18.9(o)0(e)15.1(m)0(b)18.1(r)0(y)19.6(o)0(n)15.5(i)0(c)-431.1(a)0(n)20.8(t)0(i)16.5(g)0(e)15.1(n)-438.9(\(C)20.6(EA)13.2(\),)-429.5(vi)18.9(me)17.7(nt)18.9(in)13.2(,)]TJ 0 -1.0471 TD [(cy)15.1(to)18.9(ke)20.8(ra)13.5(ti)16.5(n)-353.6(a)0(n)20.8(d)-353.6(ep)15.1(it)16.5(he)20.8(li)16.5(al)-345.7(me)17.7(mb)18.1(ra)13.5(ne)-343.4(an)15.1(ti)16.5(ge)20.8(n)-353.6(\()0(E)15.5(M)0(A)15.4(\))-355.2(w)12.4(a)0(s)]TJ 0 -1.0528 TD [(pe)15.1(rf)18(or)19.6(me)17.7(d,)-285.7(em)17.7(pl)18.9(oy)15.5(in)18.9(g)-291(s)0(t)17.7(a)0(n)15.1(d)0(a)20.8(r)0(d)-287.7(r)0(o)19.6(u)0(t)18.9(i)0(n)18.9(e)-297.1(st)17.7(re)19.2(pt)18.9(av)15.1(id)18.9(in)18.9(-b)13.9(i)13.9(o)0(t)13.2(i)0(n)]TJ 0 -1.0471 TD [(im)15.7(mu)18.1(no)15.5(hi)18.9(st)17.7(oc)15.1(he)20.8(mi)15.7(ca)14.7(l)-452.7(m)0(e)17.7(t)0(h)13.2(o)0(d)21.2(s)0(.)-446.2(M)0(e)13.2(s)0(o)20(t)0(h)18.9(e)0(l)18.4(i)0(o)13.2(m)0(a)-443.3(d)0(i)13.2(a)0(g)20.8(n)0(o)15.5(s)0(i)17.7(s)]TJ 0 -1.0528 TD [(wa)16.9(s)-326.3(c)0(o)20.8(n)0(f)13.9(i)0(r)17.3(m)0(e)17.7(d)-325.1(by)-314.5(th)18.9(e)-325.5(B)11.6(el)12.8(gi)18.9(an)-314.9(Me)18.9(so)14.4(th)18.9(el)18.4(io)18.9(ma)-318.1(Pa)15.9(ne)20.8(l.)]TJ /F4 1 Tf 0 -2.538 TD [(Study)-336.7(desig)-7.8(n)]TJ /F3 1 Tf 1.0015 -1.7527 TD [(Al)14.9(l)-287.7(t)0(h)18.9(e)-291.4(fo)19.6(rm)10.8(al)18.5(in)18.9(-f)18(ix)18.9(ed)-280.8(pa)15.1(ra)19.2(f)14.7(f)0(i)17.3(n)0(-)19.6(e)0(m)17.7(b)0(e)15.1(d)0(d)21.2(e)0(d)-280.8(t)0(i)16.5(s)0(s)18.9(u)0(e)15.1(s)-286.5(of)-282(pa)15.1(-)]TJ -1.0015 -1.0471 TD [(ti)16.5(en)20.8(ts)-528.6(wi)14.9(th)-527.4(ma)17.7(li)16.5(gn)21.2(an)15.1(t)-532.4(p)0(l)18.9(e)0(u)15.1(r)0(a)19.2(l)-538.1(me)17.7(so)14.4(t)13.9(h)0(e)15.1(l)0(i)16.5(o)0(m)18.1(a)0(,)-525.1(c)0(a)14.7(r)0(c)19.2(i)0(n)18.9(o)0(m)12.4(a)]TJ 0 -1.0527 TD [(me)17.7(ta)18.4(st)17.7(as)13.9(is)-431.9(an)15.1(d)-438.9(n)0(o)21.2(n)0(n)15.5(e)0(o)15.1(p)0(l)18.9(a)0(s)19.6(t)0(i)16.5(c)-439.3(me)12(so)20(th)18.9(el)18.4(iu)13.2(m)-442.1(w)12.4(er)13.5(e)-439.3(i)0(n)18.9(v)0(e)15.1(s)0(t)17.7(i)0(-)]TJ 0 -1.0471 TD [(ga)15.1(te)18.4(d)-279.6(w)12.4(it)16.5(h)-279.6(t)0(h)18.9(e)-280(PR)17.3(IN)15.7(S)-278.8(m)0(e)12(t)0(h)18.9(o)0(d)-269(a)0(n)15.1(d)-279.6(i)13.9(m)0(m)14.9(u)0(n)15.5(o)0(h)15.5(i)13.9(st)17.7(oc)15.1(he)15.1(mi)15.7(st)17.7(ry)]TJ 0 -1.0527 TD [(fo)19.6(r)-423.5(v)0(i)18.9(r)0(a)13.5(l)-418.6(t-)17.3(an)15.1(ti)16.5(ge)20.8(n.)-410.9(Th)17.1(e)-422.3(P)11.4(RI)14.9(NS)-414.4(re)19.2(ac)14.7(ti)16.5(on)-411.3(wa)16.9(s)-423.1(c)0(a)20.4(r)0(r)18(i)0(e)12.8(d)-416.2(ou)15.5(t)]TJ 0 -1.0471 TD [(wi)14.9(th)-339.7(th)18.9(re)13.5(e)-348.3(s)0(e)19.6(p)0(a)15.1(r)0(a)19.2(t)0(e)18.4(,)-353.2(pr)19.6(im)15.7(er)19.2(s:)-340.8(PY)12.4(V)63.6(r)0(e)13.5(v)73.2(,)-347.5(S)0(V)18.1(2)0(f)13.9(o)0(r)-338.9(a)0(n)15.1(d)-347.9(SV)69.3(re)13.5(v)]TJ 0 -1.0528 TD [(de)15.1(te)18.4(ct)18.4(in)18.8(g)-205.6(a)-206(DN)13.4(A)-209.6(s)0(e)19.6(q)0(u)21.2(e)0(n)15.1(c)0(e)-195.8(c)0(o)15.1(d)0(i)18.9(n)0(g)-200.7(f)0(o)19.6(r)-207.2(th)18.9(e)-206(v)0(i)18.9(r)0(a)13.5(l)-202.3(T)91.9(-)0(a)13.5(n)0(t)18.9(i)0(g)18.9(e)0(n)-201.1(o)0(f)]TJ 0 -1.0471 TD [(S)11.4(V)0(4)11.6(0)0(.)-410.9(T)12.2(he)15.1(se)-412.8(re)19.2(su)14.4(lt)16.5(s)-417.4(w)0(e)16.9(r)0(e)-418.9(c)0(o)20.8(r)0(r)18(e)0(l)12.8(a)0(t)18.5(e)0(d)-411.7(w)0(i)14.9(t)0(h)-413.6(i)0(m)21.4(m)0(u)12.4(n)0(o)21.2(h)0(i)18.9(s)0(t)12(o)0(-)]TJ 0 -1.0528 TD [(ch)15.1(em)17.7(is)17.7(tr)17.3(y)-393.4(f)0(o)19.6(r)-395(vi)13.2(ra)19.2(l)-395.8(a)0(n)20.8(t)0(i)16.5(g)0(e)15.1(n)0(s)-384(w)0(i)14.9(t)0(h)-385.2(t)0(h)18.9(e)-393.8(mo)12.4(us)20(e)-393.8(m)0(o)18.1(n)0(o)15.5(c)0(l)18.5(o)0(n)15.5(a)0(l)]TJ 0 -1.0471 TD [(an)15.1(ti)22.2(bo)15.5(dy)-382.8(P)11.4(a)0(b)15.1(2)0(8)21.2(0)-393.4(\(d)19.6(il)16.5(ut)18.9(io)13.2(n)-387.7(1)0(/)18.9(2)0(0)15.5(\))-389.3(\(O)15.7(nc)15.1(og)21.2(en)15.1(e,)-382.8(C)11.6(a)0(m)17.7(b)0(r)13.9(i)0(d)18.8(g)0(e)15.1(,)]TJ 0 -1.0527 TD [(MA)15.3(,)-262.1(U)0(S)18.1(A)0(\))15.7(.)-267.8(F)11.4(o)0(r)-253.5(e)0(a)14.7(c)0(h)-252.3(c)0(a)14.7(s)0(e)-253.5(a)0(t)-260.4(l)0(e)18.4(a)0(s)19.6(t)-264.9(20)15.5(0)-262.5(m)0(e)17.7(s)0(o)14.4(t)13.9(he)15.1(li)16.5(al)-254.7(ce)14.7(ll)22.2(s)-269.4(o)0(r)]TJ 0 -1.0471 TD [(ce)14.7(l)13.9(l)0(s)-392.1(o)0(f)-390.1(p)0(l)18.9(e)0(u)15.1(r)0(a)19.2(l)-401.5(ca)14.7(rc)19.2(in)18.9(om)18.1(a)-399.5(m)0(e)12(t)13.9(as)13.9(ta)18.4(si)17.7(s)-400.3(w)0(e)16.9(r)0(e)-390.5(e)0(v)20.8(a)0(l)12.8(u)0(a)20.8(t)0(e)18.4(d)-399.1(fo)13.9(r)]TJ 0 -1.0527 TD [(S)11.4(V)0(4)11.6(0)-291(sm)16.9(al)18.5(l)-299.1(t)0(-)17.3(a)0(n)20.8(t)0(i)16.5(g)0(e)15.1(n)-291(an)15.1(d)-291(P)0(R)11.6(I)0(N)21.4(S)-295.9(re)13.5(ac)20.4(ti)16.5(vi)18.9(ty)-288.4(by)-286.1(tw)20.6(o)-296.7(i)0(n)18.9(v)0(e)15.1(s)0(-)]TJ 0 -1.0471 TD [(ti)16.5(ga)20.8(to)13.2(rs)18.4(.)]TJ /F4 1 Tf 0 -2.5437 TD [(Pr)27.3(eparation)-343.3(of)-334(paraf)-7.4(fin)-337.1(sections)]TJ /F3 1 Tf 1.0015 -1.747 TD [(Pa)15.9(ra)19.2(f)20.4(f)0(i)17.3(n)-467.4(se)19.6(ct)18.4(io)13.2(ns)-452.3(5)-467.4(m)0(m)-457.4(t)0(h)18.9(i)0(c)18.4(k)-461.7(we)16.9(re)-458.8(cu)20.8(t,)-458.8(fl)17.3(oa)20.8(te)12.8(d)-461.7(o)0(n)-456.8(a)]TJ -1.0015 -1.0527 TD [(pr)19.6(ot)13.2(ei)18.4(n-)19.6(fr)18(ee)-395(w)12.4(a)0(t)12.8(e)0(r)-390.5(b)0(a)20.8(t)0(h)-390.9(a)0(t)-391.3(3)0(7)]TJ /F9 1 Tf 11.6542 0 TD 0 Tw (8)Tj /F3 1 Tf 0.3756 0 TD [(C)-398.1(a)0(n)15.1(d)-393.4(ad)15.1(he)15.1(re)19.2(d)-399.1(o)0(n)-388.5(o)0(r)25.3(g)0(a)20.8(n)0(o)15.5(-)]TJ -12.0298 -1.0471 TD [(si)17.7(la)18.4(ne)15.1(-c)19.2(oa)15.1(te)18.4(d)-336.5(g)0(l)18.9(a)0(s)13.9(s)-337.7(sl)17.7(id)18.9(es)-327.5(\(p)13.9(re)19.2(tr)17.3(ea)20.4(te)12.8(d)-336.5(f)0(o)19.6(r)-338.1(20)-331.6(mi)15.7(n)-336.5(w)12.4(it)16.5(h)-336.5(3)0(%)]TJ 0 -1.0527 TD [(3-)19.6(am)12(in)18.9(op)21.2(ro)13.9(py)21.2(lt)16.5(ri)17.3(et)18.5(ho)15.5(xy)15.5(si)17.7(la)18.4(n)-251.2(\()0(S)20.4(i)0(g)13.2(m)13.2(a)0(,)-246.2(S)11.4(t)-253.5(L)12.2(ou)15.5(is)17.7(,)-250.8(M)0(O)15.3(,)-250.8(US)18.1(A\))]TJ 0 -1.0471 TD [(in)-282.7(ac)20.4(et)18.5(on)15.5(e\))19.2(.)-290.6(A)0(f)15.7(t)0(e)18.4(r)-292.6(dr)19.6(yi)18.9(ng)-280.4(at)-283.1(37)]TJ /F9 1 Tf 11.99 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD [(C)-290(t)0(h)18.9(e)-291.4(sl)17.7(id)18.9(es)-282(we)16.9(re)-282.4(ba)15.1(ke)20.8(d)-291(a)0(t)]TJ -12.3656 -1.0527 TD (60)Tj /F9 1 Tf 0.9845 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD 0.3248 Tc [(Cf)324.8(o)344.4(r)-1.9(2h)5.4(a)324.8(n)339.9(ds)324.8(t)342.5(o)324.8(r)344.4(e)324.8(d)9.9(d)324.8(u)340.3(s)324.8(t)342.5(-)324.8(f)342.8(r)324.8(e)344(e)324.8(.)]TJ /F4 1 Tf -1.36 -2.538 TD 0 Tc [(Prime)-10.1(d)]TJ /F3 1 Tf 3.255 0 TD [(in)-334(sit)-8.2(u)]TJ /F4 1 Tf 2.868 0 TD [(techni)-10.1(que)]TJ -6.123 -1.7527 TD [(Pr)27.3(ehybridiza)-9.9(tion)-485.8(tr)29(eatmen)-11.7(t.)]TJ /F3 1 Tf 11.6827 0 TD [(E)12.2(a)0(c)14.7(h)-473.1(se)19.6(ct)12.8(i)13.9(o)0(n)-468.2(w)12.4(as)-464.1(de)15.1(wa)16.9(xe)15.1(d)]TJ -11.6827 -1.0471 TD [(in)-595.7(t)13.9(w)0(o)-597.3(r)0(i)17.3(n)0(s)20(e)0(s)-594.9(o)0(f)-594.9(x)0(y)21.2(l)0(e)18.4(n)0(e)-593.8(s)0(u)14.4(b)0(s)20(t)0(i)16.5(t)0(u)18.9(t)0(e)-596.1(P)11.4(ar)13.5(as)19.6(ol)18.9(ve)-593.8(\(P)14.7(ro)19.6(sa)19.6(n,)]TJ 0 -1.0528 TD [(Gh)17.3(en)15.1(t,)-327.9(B)11.6(e)0(l)12.8(g)0(i)18.9(u)0(m)18.1(\))0(,)-327.1(a)0(n)15.1(d)-336.5(re)19.2(hy)15.5(dr)19.6(at)12.8(ed)-326.3(th)18.9(ro)13.9(ug)21.2(h)-336.5(a)-342.6(gr)19.6(ad)20.8(ed)-332(se)19.6(ri)17.3(es)]TJ 0 -1.0471 TD [(of)-316.1(al)18.4(co)15.1(ho)21.2(l)-327.5(t)0(o)-311.2(w)0(a)16.9(t)0(e)12.8(r)60.2(.)]TJ 26.1993 66.1583 TD [(Th)17.1(e)-257.2(t)0(i)16.5(s)0(s)18.9(u)0(e)-246.6(w)12.4(as)-247.8(th)18.8(en)-246.6(di)18.9(ge)15.1(st)17.7(ed)-240.9(wi)14.9(th)-242.9(pe)15.1(ps)20(in)-248.6(\(4)-242.1(mg)]TJ /F10 1 Tf 18.5512 0.1992 TD (.)Tj /F3 1 Tf 0.2675 -0.1992 TD (mL)Tj 7.472 0 0 6.641 520.327 776.069 Tm (-1)Tj 9.963 0 0 9.963 528.888 771.647 Tm (in)Tj -22.0395 -1.0471 TD [(0.)15.9(2)-416.2(N)-420.1(ac)20.4(et)12.8(ic)-408.3(ac)20.4(id)13.2(;)-418.6(S)11.4(ig)18.9(ma)17.7(\))-417.8(s)0(o)14.4(l)0(u)18.9(t)0(i)16.5(o)0(n)-405.6(f)0(o)13.9(r)-417.8(45)-405.6(mi)15.7(n)-421.9(a)0(t)-408.3(3)0(7)]TJ /F9 1 Tf 21.516 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD (C.)Tj -21.8915 -1.0528 TD [(Af)15.7(te)12.8(r)-423.5(w)0(a)16.9(s)0(h)20(i)0(n)13.2(g)-421.9(tw)14.9(o)-421.9(t)0(i)16.5(m)0(e)17.7(s)-428.7(in)-413.6(di)18.8(st)17.7(il)16.5(le)18.4(d)-427.6(w)0(a)16.9(t)0(e)18.5(r)37.5(,)-421.5(t)0(h)13.2(e)-422.3(se)19.6(ct)12.8(io)18.8(ns)]TJ 0 -1.0471 TD [(we)16.9(re)-367.7(de)15.1(hy)21.2(dr)13.9(at)18.5(ed)-360.4(in)-368.1(a)-371.1(g)0(r)13.9(a)0(d)20.8(e)0(d)-366.1(s)0(e)19.6(r)0(i)17.3(e)0(s)-367.3(o)0(f)-361.6(a)0(l)12.8(c)0(o)20.8(h)0(o)15.5(l)-373(to)-362.4(is)17.7(op)15.5(ro)19.6(-)]TJ 0 -1.0528 TD [(py)15.5(la)18.4(lc)18.4(oh)15.5(ol)-316.9(10)21.2(0%)-316.9(an)15.1(d)-325.1(a)0(i)18.4(r)-321(dr)14(ie)18.4(d.)]TJ /F4 1 Tf 0 -1.838 TD [(Pr)-6.8(imed)]TJ /F3 1 Tf 3.3859 0 TD [(in)-459.2(sit)-8.2(u)]TJ /F4 1 Tf 3.1184 0 TD [(techni)-10.1(que.)]TJ /F3 1 Tf 4.5752 0 TD -0.0146 Tc [(T)-14.6(h)2.5(e)-465.3(s)-14.6(e)5(c)-14.6(t)3.8(i)-14.6(on)-14.6(s)-455.5(w)-14.6(er)-14.6(e)-456.3(d)-14.6(en)-14.6(a)6.2(t)-14.6(ur)-14.6(e)4.6(d)]TJ -11.0795 -1.0527 TD 0 Tc [(in)-425(fo)19.6(rm)16.5(am)17.7(id)13.2(e)-428(s)0(o)14.4(l)13.9(ut)13.2(io)18.9(n)-427.6(a)0(t)-425.4(9)0(4)]TJ /F9 1 Tf 11.5575 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD [(C)-432.3(f)0(o)14(r)-429.2(5)-427.6(m)0(i)15.7(n)0(.)-422.2(T)0(h)17.1(e)-428(so)14.4(lu)18.8(ti)16.5(on)]TJ -11.9331 -1.0471 TD [(is)-386.4(pr)19.6(ep)15.1(ar)19.2(ed)-383.2(as)-384.4(fo)13.9(ll)22.2(ow)11.6(s:)-380.7(70)-382.8(mL)-384.4(de)15.1(io)18.9(ni)18.9(ze)14.7(d)-393.4(f)0(o)19.6(r)0(m)16.5(a)0(m)17.7(i)0(d)18.8(e)0(,)-382.8(1)0(0)]TJ 0 -1.0527 TD [(mL)-424.2(20)]TJ /F9 1 Tf 2.7827 0 TD (6)Tj /F3 1 Tf 1.4283 0 TD [(SS)17.1(C,)-421.3(10)-422.6(mL)-424.2(S)11.4(o)]TJ 6.2824 0 TD [(\310)-76.3(r)0(e)19.2(n)0(s)20(e)0(n)-423(b)0(u)15.5(f)20.4(fe)19.2(r)-434.8(p)0(H)-420.9(7)0(.)21.6(0)-433.3(\(0)19.6(.2)-422.3(M)]TJ -10.4934 -1.0471 TD [(Na)16.9(H)]TJ 7.472 0 0 6.641 327.742 667.956 Tm (2)Tj 9.963 0 0 9.963 331.37 669.656 Tm (PO)Tj 7.472 0 0 6.641 343.899 667.956 Tm (4)Tj 9.963 0 0 9.963 347.527 669.656 Tm 0.3216 Tc [(;0)321.6(.)337.5(2)-3.5(MN)321.6(a)]TJ 7.472 0 0 6.641 392.315 667.956 Tm 0 Tc (2)Tj 9.963 0 0 9.963 396 669.656 Tm -0.0124 Tc [(HP)-12.4(O)]TJ 7.472 0 0 6.641 415.559 667.956 Tm 0 Tc (4)Tj 9.963 0 0 9.963 419.187 669.656 Tm [(\),)-315.7(10)-314.5(mL)-316.1(di)18.9(st)17.7(il)16.5(le)18.4(d)-325.1(w)0(a)16.9(t)0(e)18.4(r)60.2(.)]TJ -10.0267 -1.0527 TD [(Th)17.1(e)-302.8(s)0(e)14(c)0(t)18.4(i)0(o)18.9(n)0(s)-292.9(w)0(e)16.9(r)0(e)-293.8(t)0(h)18.9(e)0(n)-292.2(w)0(a)16.9(s)0(h)14.4(e)0(d)-292.2(i)0(n)-288.4(a)0(n)-292.2(a)0(s)13.9(c)0(e)20.4(n)0(d)15.5(i)0(n)18.9(g)-302.4(gr)19.6(ad)15.1(ed)]TJ -1.0015 -1.0471 TD [(se)13.9(ri)17.3(es)-270.6(of)-276.3(ic)18.4(e)-280(c)0(o)15.1(l)0(d)-271.4(a)0(l)18.4(c)0(o)15.1(h)0(o)21.2(l)-282(\(i)17.3(so)14.4(pr)19.6(op)15.5(yl)18.8(al)18.5(co)15.1(ho)21.2(l)-282(7)0(5)15.5(%)-282(90)21.2(%)-282(a)0(n)15.1(d)]TJ 0 -1.0527 TD [(10)15.5(0%)18.9(\))-326.7(a)0(t)-317.3(-)0(1)19.6(8)]TJ /F9 1 Tf 5.2524 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD [(C)-329.8(f)0(o)19.6(r)-326.7(3)-319.4(m)0(i)15.7(n)-325.1(in)-316.9(ea)20.4(ch)-314.9(al)12.8(co)20.8(ho)15.5(l)-321.8(d)0(i)18.9(l)0(u)13.2(t)0(i)22.2(o)0(n)15.5(.)]TJ -4.6264 -1.0471 TD [(Th)17.1(e)-319.8(s)0(e)19.6(c)0(t)18.4(i)0(o)18.9(n)0(s)-310(w)0(e)16.9(r)0(e)-305.1(a)0(i)12.8(r)-315.3(dr)19.6(ie)18.4(d)-319.4(a)0(n)20.8(d)-313.7(im)15.7(me)12(di)18.9(at)18.5(el)18.5(y)-313.8(i)0(n)13.2(c)0(u)20.8(b)0(a)15.1(t)0(e)18.4(d)]TJ -1.0015 -1.0527 TD [(dr)13.9(y)-256.8(a)0(t)-249(9)0(0)]TJ /F9 1 Tf 3.4997 0 TD (8)Tj /F3 1 Tf 0.3756 0 TD [(C)-255.9(f)0(o)13.9(r)-258.4(1)-251.2(m)0(i)15.7(n)-256.8(us)14.4(in)18.9(g)-256.8(a)-257.2(ho)21.2(t)-259.2(p)0(l)18.9(a)0(t)18.4(e)-257.2(\(M)17.7(ed)15.1(it)16.5(e,)-246.2(B)11.6(u)0(r)25.3(g)0(d)15.5(o)0(r)19.6(f)0(,)]TJ -3.8753 -1.0471 TD [(Ge)16.9(rm)10.8(an)20.8(y\))19.6(.)]TJ /F4 1 Tf 0 -1.8437 TD [(Pr)27.3(eparat)-8.6(ion)-357.5(of)-356.7(p)-6.5(rimed)]TJ /F3 1 Tf 9.4918 0 TD [(in)-362.4(situ)]TJ /F4 1 Tf 2.9136 0 TD [(r)26.5(eaction)-363.7(mix.)]TJ /F3 1 Tf 5.6735 0 TD -0.0144 Tc [(Th)-14.4(e)-357.8(f)-14.4(o)5.2(l)-14.4(lo)-14.4(w)2.9(-)]TJ -18.0789 -1.0471 TD 0 Tc [(in)13.2(g)-330.8(c)0(o)20.8(m)0(p)12.4(o)0(n)21.2(e)0(n)15.1(t)0(s)-323.8(w)12.4(er)13.5(e)-331.2(m)0(i)15.7(x)0(e)20.8(d)-330.8(in)-322.6(an)-326.3(ep)20.8(pe)15.1(nd)21.2(or)14(f)-332.4(v)0(i)18.9(a)0(l)18.4(:)-333.2(13)]TJ /F8 1 Tf 21.6298 0 TD (m)Tj /F3 1 Tf 0.5691 0 TD (L)Tj -22.1988 -1.0528 TD [(di)13.2(st)17.7(il)16.5(l)13.9(e)0(d)-428.7(w)0(a)16.9(t)0(e)18.4(r)37.5(,)-432.9(3)]TJ /F8 1 Tf 7.2668 0 TD (m)Tj /F3 1 Tf 0.5634 0 TD [(L)-431.7(P)11.4(RI)14.9(NS)-425.8(bu)15.5(f)20.4(f)0(e)19.2(r)-434.8(\(1)13.9(0)]TJ /F9 1 Tf 8.3253 0 TD (6)Tj /F3 1 Tf 1.434 0 TD [(co)20.8(nc)15.1(en)15.1(t)13.9(r)0(a)13.5(t)0(e)18.4(d)0(;)]TJ -17.5895 -1.0471 TD [(50)-325.9(mM)-331(KC)12.6(l)13.9(,)-336.1(10)-325.9(mM)-331(TR)18.1(IS)14.7(-H)15.7(C)11.6(l)0(,)-327.9(1)0(.)21.6(5)-336.5(mM)-331(Mg)19.3(Cl)]TJ 7.472 0 0 6.641 501.732 555.477 Tm (2)Tj 9.963 0 0 9.963 505.36 557.178 Tm 0.3361 Tc [(,0)336.1(.)357.7(0)336.1(1)351.6(%)]TJ -19.6779 -1.0528 TD 0 Tc [(bo)15.5(vi)18.9(ne)-349.1(se)19.6(ru)13.9(m)-356.7(a)0(l)18.5(b)0(u)21.2(m)0(i)15.7(n)-359.3(\()-355.2(B)0(S)17.3(A)0(\))15.7(\))0(,)-344.2(3)]TJ /F8 1 Tf 13.9874 0 TD (m)Tj /F3 1 Tf 0.5634 0 TD 0.3577 Tc [(L1)357.7(0)]TJ /F9 1 Tf 1.9405 0 TD 0 Tc (6)Tj /F3 1 Tf 1.36 0 TD [(co)20.8(nc)15.1(en)20.8(tr)17.3(at)18.4(ed)]TJ -17.8512 -1.0471 TD [(DI)15.7(G-)15.7(PR)17.3(IN)15.7(S)-694.2(l)0(a)18.4(b)0(e)15.1(l)13.9(li)16.5(ng)-684.4(mi)15.7(x)-689.3(\()0(5)13.9(0)0(0)]TJ /F8 1 Tf 14.2947 0 TD (m)Tj /F3 1 Tf 0.5634 0 TD 0.697 Tc [(Me)697(a)717.4(c)697(h)12.2(o)697(f)16.7(d)697(A)816.7(T)697(P)817.3(,)]TJ -14.858 -1.0527 TD 0 Tc [(dC)16.5(TP)120.3(,)-398.7(a)0(n)15.1(d)-399.1(dG)17.3(TP)17.9(;)-401.5(5)0(0)]TJ /F8 1 Tf 9.0594 0 TD (m)Tj /F3 1 Tf 0.5634 0 TD [(M)-401.1(D)0(I)15.7(G)12.4(-d)13.9(UT)18.9(P;)-395.8(50)21.2(%)-401.5(g)0(l)18.8(y)0(c)15.1(e)0(r)19.2(o)0(l)18.9(\))0(,)-395.4(5)]TJ /F8 1 Tf -9.6227 -1.0471 TD (m)Tj /F3 1 Tf 0.5691 0 TD [(L)-482.9(P)0(R)11.6(I)0(N)21.4(S)-489.4(ol)18.9(ig)13.2(on)21.2(uc)15.1(le)18.4(ot)18.9(id)18.8(e)-490.6(p)0(r)19.6(i)0(m)15.7(e)0(r)-481.5(\()0(e)19.2(i)0(t)16.5(h)0(e)15.1(r)-486.1(po)15.5(si)17.7(t)13.9(i)0(v)13.2(e)-484.9(co)15.1(n-)]TJ -0.5691 -1.0527 TD [(tr)17.3(ol)-362.4(pr)19.6(im)15.7(er)-362(or)-361.6(S)11.4(V)0(4)11.6(0)0(-)19.6(s)0(p)20(e)0(c)14.7(i)0(f)17.3(i)0(c)-362.8(o)0(l)18.9(i)0(g)18.8(o)0(n)21.2(u)0(c)15.1(l)0(e)18.4(o)0(t)18.9(i)0(d)13.2(e)-365.4(pr)13.9(im)15.7(er)47.7(,)-364.6(5)0(0)15.5(0)]TJ 0 -1.0471 TD [(pm)12.4(ol)18.9(\),)-349.9(1)]TJ /F8 1 Tf 3.7899 0 TD (m)Tj /F3 1 Tf 0.5634 0 TD 0.3577 Tc [(L4)357.7(5)373.2(0)]TJ /F8 1 Tf 2.8054 0 TD 0 Tc (m)Tj /F3 1 Tf 0.5634 0 TD 0.3612 Tc [(Md)361.2(T)378.3(T)373.4(P)469.3(,)2.3(5)]TJ /F8 1 Tf 4.837 0 TD 0 Tc (m)Tj /F3 1 Tf 0.5634 0 TD [(L)-272.4(T)69.1(aq)-349.1(DN)13.4(A)-357.5(p)0(o)15.5(l)0(y)18.9(m)0(e)17.7(r)0(a)13.5(s)0(e)]TJ -13.1224 -1.0527 TD [(\(0)13.9(.5)-314.1(U)]TJ /F10 1 Tf 2.5835 0.1992 TD (.)Tj /F8 1 Tf 0.2675 -0.1992 TD (m)Tj /F3 1 Tf 0.5634 0 TD [(L\))15.5(.)]TJ -2.4128 -1.0471 TD [(Al)14.9(l)-663.2(r)0(e)19.2(a)0(g)15.1(e)0(n)20.8(t)0(s)-653.8(w)0(e)16.9(r)0(e)-652.3(p)0(u)15.5(r)0(c)19.2(h)0(a)15.1(s)0(e)19.6(d)-660.9(fr)18(om)-653.4(Bo)16.5(eh)20.8(ri)11.6(ng)21.2(er)24.9(-M)17.7(a-)]TJ -1.0015 -1.0528 TD [(nn)15.5(he)15.1(i)13.9(m)-538.8(\(M)17.7(an)20.8(nh)15.5(ei)18.4(m,)-527.8(Ge)16.9(rm)16.5(an)20.8(y\))-526.7(un)15.5(le)18.4(ss)-521.8(ot)18.9(he)15.1(rw)15.7(is)17.7(e)-530.4(i)0(n)13.2(d)0(i)18.9(c)0(-)]TJ T* [(at)12.8(ed)-428.7(Th)17.1(e)-445(f)0(o)19.6(l)0(l)16.5(o)0(w)17.3(i)0(n)18.9(g)-444.6(pr)19.6(im)15.7(er)13.5(s)-440.1(s)0(e)19.6(q)0(u)15.5(e)0(n)15.1(c)0(e)20.4(s)-445.8(i)13.9(n)-444.6(br)19.6(ac)14.7(ke)15.1(t)13.9(s)-445.8(we)16.9(re)]TJ 0 -1.0471 TD [(us)14.4(ed)-553.9(in)-561.6(se)19.6(pa)15.1(ra)19.2(te)-556.3(re)13.5(ac)20.4(ti)16.5(on)15.5(s)-565.3(f)0(o)19.6(r)-565.7(SV)18.1(40)-559.2(de)15.1(te)18.5(ct)18.4(io)18.9(n:)-561.6(S)11.4(V)57.9(re)19.2(v)]TJ 0 -1.0528 TD [(\(G)15.7(CA)120.7(TG)13.2(AC)18.3(TC)18.1(AA)13.4(AA)13.4(A)12.4(A)0(C)12.6(T)12.2(T)80.5(A)0(G)13.4(C)11.6(AA)115.8(T)12.2(T)0(C)12.4(T)12.2(G\))15.7(,)-279.2(S)11.4(V2)-272.9(fo)19.6(r)]TJ 0 -1.0471 TD [(\(C)14.9(TT)18.7(TG)13.2(GA)19.1(GG)13.4(CT)18.1(TC)18.1(TG)13.2(GG)19.1(A)109.1(T)0(G)18.9(C)0(A)12.6(A)0(C)18.3(T)0(\))-553.5(a)0(n)20.8(d)-564.1(P)11.4(Y)0(V)64.6(r)0(e)19.2(v)]TJ 0 -1.0528 TD [(\(G)15.7(GA)13.4(AA)19.1(GT)13.2(CT)18.1(TT)87(A)12.4(G)0(G)13.4(G)0(T)18.9(C)0(T)12.4(T)12.2(CT)86.4(A)12.4(C)0(C)11.8(\))0(.)-196.2(T)0(h)17.1(e)-206(la)18.4(tt)16.5(er)-202.7(is)-198.6(de)20.8(-)]TJ 0 -1.0471 TD [(te)12.8(ct)18.5(in)18.9(g)-256.8(a)-262.9(re)19.2(gi)13.2(on)-246.2(\(4)13.9(42)21.2(5\261)15.5(44)21.2(02)-251.9(ba)15.1(se)-247.8(pa)15.1(ir)17.3(s)-258(\()0(b)19.6(p)0(\))13.9(\))-258.4(co)15.1(di)18.9(ng)-251.9(fo)19.6(r)-258.4(T)91.9(-)]TJ 0 -1.0527 TD [(an)15.1(ti)16.5(ge)20.8(n,)-325.5(w)12.4(h)0(i)13.2(c)0(h)-320.6(i)0(s)-323.8(c)0(o)20.8(m)0(m)14.9(o)0(n)-325.9(t)13.9(o)-330.8(t)0(h)13.2(e)-331.2(B)11.6(K)-334.8(vi)18.9(ru)19.6(s,)-326.7(JC)-320.4(vi)18.8(ru)14(s)-326.3(a)0(n)15.1(d)]TJ 0 -1.0471 TD [(SV)12.4(40)-382.8(vi)18.9(ru)19.6(s.)-383.6(T)12.2(h)0(e)-383.2(f)0(o)13.9(r)0(m)16.5(e)0(r)-379.1(t)0(w)14.9(o)-393.4(pr)19.6(im)15.7(er)19.2(s)-394.6(a)0(r)19.2(e)-393.8(S)11.4(V)0(4)17.3(0)-393.4(sp)20(ec)14.7(if)17.3(ic)18.5(.)]TJ 0 -1.0527 TD [(Th)17.1(e)-428(S)11.4(V)57.9(r)0(e)19.2(v)-421.9(pr)13.9(im)15.7(er)-413.2(is)-414.8(ho)15.5(mo)18.1(lo)18.9(go)15.5(us)-412.4(to)-419.3(th)18.9(e)-422.3(4)0(3)15.5(9)0(9)21.2(\261)0(4)15.5(3)0(7)21.2(2)-427.6(bp)]TJ 0 -1.0471 TD 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[(be)15.1(dd)21.2(ed)-195.4(29)15.5(3)-205.6(c)0(e)14.7(l)0(l)16.5(s)-201.1(we)11.2(re)-197(us)20(ed)-195.4(as)-202.3(a)-206(p)0(o)21.2(s)0(i)17.7(t)0(i)16.5(v)0(e)-195.4(c)0(o)15.1(n)0(t)18.9(r)0(o)13.9(l)0(.)-197(F)11.4(or)19.6(ma)12(li)16.5(n-)]TJ 0 -1.0471 TD [(fi)17.3(xe)15.1(d)-313.7(a)0(n)20.8(d)-313.7(pa)15.1(ra)19.2(f)20.4(f)0(i)17.3(n)0(-)13.9(e)0(m)17.7(b)0(e)15.1(d)0(d)21.2(e)0(d)-303.5(p)0(l)13.2(a)0(c)20.4(e)0(n)15.1(t)0(a)18.4(l)-316.1(ti)22.2(ss)13.2(ue)-303.5(wa)16.9(s)-314.9(u)0(s)20(e)0(d)-309.2(a)0(s)]TJ 0 -1.0528 TD [(ne)15.1(ga)20.8(ti)16.5(ve)-314.9(co)15.1(nt)18.9(ro)19.6(l)-327.5(t)0(i)16.5(s)0(s)18.9(u)0(e)20.8(.)]TJ /F6 1 Tf 9.8617 -2.6006 TD (Results)Tj /F3 1 Tf -8.8602 -1.9519 TD [(Th)17.1(er)19.2(e)-507.6(w)12.4(as)-498.2(br)19.6(ig)18.9(ht)-499(fi)17.3(ne)20.8(ly)-499(gr)19.6(an)15.1(ul)18.9(ar)-492.9(nu)15.5(cl)18.4(ea)20.4(r)-508.8(s)0(t)17.7(a)0(i)18.4(n)0(i)18.9(n)0(g)-496.6(i)0(n)]TJ -1.0015 -1.0527 TD 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[(S)11.4(V)0(4)11.6(0)-251.2(vi)18.9(ru)19.6(s,)-247(re)19.2(ve)15.1(al)18.4(ed)-240.9(nu)15.5(cl)18.4(ea)14.7(r)-252.7(s)0(i)17.7(g)0(n)21.2(a)0(l)18.4(s)-252.3(in)-248.6(al)18.4(l)-253.5(t)0(h)18.9(o)0(s)20(e)-257.2(ca)20.4(se)19.6(s)-258(t)13.9(ha)15.1(t)]TJ 0 -1.0528 TD [(te)18.4(st)17.7(ed)-326.3(po)21.2(si)17.7(ti)16.5(ve)-326.3(w)12.4(i)0(t)16.5(h)-330.8(th)13.2(e)-331.2(P)11.4(YV)64.6(re)19.2(v)-330.8(p)0(r)13.9(i)0(m)21.4(e)0(r)-327.9(\()0(f)18(i)0(g)18.8(.)-330.4(1\).)-333.6(The)-348.2(per-)]TJ 0 -1.0471 TD [(centag)-8.2(e)-524.7(o)0(f)-526.7(positive)-532.7(cell)-8.6(s)-525.5(varied)-532.9(considerabl)-11.5(y)-524.3(betw)-9.8(een)]TJ 0 -1.0528 TD [(different)-438.3(tumours)-440.4(\(tabl)-10.6(e)-433.7(1\).)-436(The)-439.3(percent)-10.5(age)-435.6(of)-441.3(positive)]TJ 0 -1.0471 TD [(neopla)-7.8(stic)-407.7(cells)-408.9(ranged)-411(from)-407.7(2%)-407.9(in)-402.2(an)-406(epitheli)-10.7(al)-402.7(meso)-7.8(-)]TJ 25.1977 43.7603 TD [(thel)-8.2(ioma)-350.4(to)-345.3(nearl)-8.6(y)-347.9(100%)-346.9(in)-351(a)-342.6(mese)-8.2(nchym)-7.4(al)-345.7(meso)-7.8(thelio-)]TJ 0 -1.0755 TD [(ma.)-358.3(Stromal)-361.6(elements)-363.5(such)-351.8(as)-355.9(blood)-353.3(ve)-7.6(ssels,)-357.7(adipocytes)]TJ T* [(and)-310(inflammato)-12.1(ry)-304.7(cell)-8.6(s)-297.9(wer)-8.2(e)-302.8(found)-307(not)-306.3(to)-305.5(be)-303.5(positive)-9.1(.)-302(I)0(n)]TJ T* [(all)-371.7(the)-363.6(cases)-365.9(that)-372.4(tested)-369.9(positive)-367.7(for)-369.7(SV40)-364.7(or)-367.3(SV40-)-8.6(like)]TJ T* [(DN)-9.4(A,)-528.6(cy)-7.6(toplasm)-9.2(ic)-533.5(and)-531.9(nuclea)-8.2(r)-531.6(immunor)-9.9(eacti)-9.8(vity)-537(was)]TJ T* [(found)-449.3(for)-443.6(the)-443.3(small)-445.8(t-ant)-10.6(igen)-444(with)-444.2(the)-443.2(monoc)-7.4(lonal)-447.2(an-)]TJ T* [(tibo)-7.8(dy)-354.3(pAb2)-7(80)-360(\(fig.)-361.8(2\).)-356.3(The)-359.6(amoun)-7.4(t)-356(o)0(f)-355.9(imm)-11(unoreac)-8.1(tive)]TJ T* [(cell)-8.6(s)-286.5(was)-286.6(compa)-7.8(rable)-287.4(to)-288.4(those)-286.6(with)-290.6(the)-289.6(nuclear)-289.4(signa)-7.8(l)-282(for)]TJ T* [(SV40-)-8.6(like)-480.5(DNA)-486.4(in)-476.2(a)-479.2(given)-484.6(sample)-484.4(\(table)-484.1(1\).)-481.5(Positive)]TJ 0 -1.0698 TD [(PRI)-7.8(NS)-260.7(contr)-8.2(ol)-260(react)-9(ion)-260.7(with)-262.1(the)-261.2(pa)-7.6(n)-256.8(chrom)-9(osome)-264.6(primer)]TJ 0 -1.0755 TD [(speci)-8.2(fic)-433.5(for)-438(all)-434.2(human)-439.8(chrom)-9(osomes)-437.3(displa)-10.9(yed)-429.5(in)-436.4(neo-)]TJ T* [(plast)-10.2(ic)-596.1(mesothel)-10.3(ial)-599.3(cells)-596.7(of)-600.6(every)-598(meso)-7.8(theliom)-10.3(a)-593(case)]TJ T* [(mul)-7.8(tiple)-307.3(nuclea)-8.2(r)-304(signals)-308.8(indi)-7.8(cating)-306.1(the)-306.7(detection)-311.1(of)-304.7(chro-)]TJ T* [(moso)-7.4(mal)-338.3(DNA)-344.1(\(fig.)-339.1(3\).)-339.2(The)-336.9(293)-338(cells)-340.6(acting)-340.2(as)-338.9(positive)]TJ T* [(con)-8.4(trols)-415.4(wer)-8.2(e)-410.9(clear)-9(ly)-413.6(positive)-418.9(wi)-7.8(th)-413.6(the)-414.8(PRINS)-422.2(reaction)]TJ T* [(detect)-10.6(ing)-294.9(the)-295.3(SV40)-296.4(DNA)-298.6(and)-292.9(displ)-9.8(ayed)-294.1(cytoplasm)-11.1(ic)-294.5(and)]TJ T* [(nuc)-8.4(lear)-514.3(immunor)-9.9(eactivit)-11.1(y)-512.9(for)-511.9(small)-519.8(t-antigen)-522.9(with)-512.5(the)]TJ T* [(monoc)-7.4(lonal)-339(anti)-8.2(body)-333.1(Pa)-6.8(b280.)]TJ ET q 228.2595 0 0 161.2346 309.3164 617.8959 cm /Im1 Do Q BT 7.97 0 0 7.97 309.316 606.274 Tm [(Fig.)-334.3(1.)-324.1(\261)-332.2(Some)-443.9(nuclei)-438.3(of)-444.1(neoplastic)-439.5(mesotheli)12.3(al)-445.3(cells)-448.7(display)-438.4(nuclear)]TJ 0 -1.067 TD [(staining)-324.8(for)-321.3(simian)-319.8(vacuolating)-322.4(virus)-320.5(40)-323(\(SV40\))-315.2(deoxyribonucle)13.3(ic)-324.4(acid)]TJ 0 -1.0599 TD [(\(DNA\))-367.2(with)-368.8(the)-365(primed)]TJ /F4 1 Tf 10.0295 0 TD [(in)-368.1(situ)]TJ /F3 1 Tf 2.9306 0 TD [(\(PRINS\))-365.6(method)-366(in)-368.1(an)-364.7(epithelial)-364.9(mes-)]TJ -12.9601 -1.0599 TD [(othelioma)-356.4(\(SV2for)-348.9(primer\).)-356.3(The)-358.2(majority)-352.6(of)-358.8(nuclei)-352.9(remains)-357.2(unstained.)]TJ 0 -1.067 TD [(\(Internal)-332.9(scale)-331.5(bar)]TJ /F8 1 Tf 7.2625 0 TD (=)Tj /F3 1 Tf 0.5548 0 TD (10)Tj /F8 1 Tf 1.3302 0 TD (m)Tj /F3 1 Tf 0.569 0 TD (m.\))Tj ET 1 i 308.976 297.241 227.168 -0.51 re f 308.976 271.332 227.168 -0.51 re f 308.976 112.932 227.168 -0.51 re f BT /F2 1 Tf 8.966 0 0 8.966 308.976 339.477 Tm [(Table)-331.9(1.)-336.7(\261)-858(Results)-523.2(of)-520.1(the)-524.5(primed)]TJ /F11 1 Tf 16.0665 0 TD [(in)-526.2(situ)]TJ /F2 1 Tf 3.3701 0 TD [(\(PRINS\))-527.5(and)]TJ -19.4366 -1.0559 TD [(immunohistochemical)-295.3(investigation)-288.8(for)-294.6(simian)-288.4(vacuolating)]TJ T* [(virus)-419.8(40)-419.8(\(SV40\))-419.7(deoxyribonucleic)-418.1(acid)-422.6(\(DNA\))-418.6(and)-417.9(SV40)]TJ T* 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[(ca)14.7(se)19.6(s)-263.7(w)0(e)16.9(r)0(e)-259.6(a)0(l)18.5(s)0(o)-258.8(f)0(o)19.6(u)0(n)15.5(d)-262.5(no)15.5(t)-264.9(t)0(o)-254.3(b)0(e)-258(i)0(m)15.7(m)0(u)18.1(n)0(o)15.5(r)0(e)19.2(a)0(c)14.7(t)0(i)16.5(v)0(e)-252.3(f)0(o)13.9(r)-264.1(SV)18.1(40)]TJ 0 -1.0471 TD -0.0186 Tc [(s)-18.6(m)-1.7(a)-18.6(l)-0.2(l)-346.1(t)-18.6(-a)-18.6(nt)-18.6(ig)-18.6(e)-3.5(n)-18.6(.)-332.7(N)-18.6(eg)-18.6(at)-18.6(iv)-18.6(e)-333.5(c)-18.6(o)-3.5(n)-18.6(tr)-18.6(ol)-18.6(s)-336.7(r)-18.6(em)-18.6(a)-6.6(i)-18.6(ne)-18.6(d)-333.5(u)-18.6(n)-3.1(s)-18.6(ta)-18.6(in)-18.6(ed)-18.6(.)]TJ /F6 1 Tf 9.1675 -4.0403 TD 0 Tc [(Discuss)-9.1(ion)]TJ /F3 1 Tf -8.1659 -2.3502 TD [(In)-390.1(th)13.2(is)-392.1(st)17.7(ud)21.2(y)73.2(,)-404.4(S)11.4(V)0(4)17.3(0)-399.1(or)-395.8(S)11.4(V)0(4)17.3(0)0(-)13.9(l)13.9(i)0(k)13.2(e)-399.5(DN)19.1(A)-317.7(w)0(a)11.2(s)-400.3(fo)19.6(un)21.2(d)-404.8(i)0(n)]TJ -1.0015 -1.0528 TD [(~6)18.1(0%)-231.5(of)-230.7(th)18.9(e)-240.2(i)0(n)18.8(v)0(e)15.1(s)0(t)17.7(i)0(g)18.9(a)0(t)18.5(e)0(d)-235.2(c)0(a)20.4(s)0(e)19.6(s)0(.)-230(I)0(n)-230.7(c)0(o)15.1(n)0(t)18.9(r)0(a)13.5(s)0(t)17.7(,)-233.7(no)15.5(n-)19.6(ne)15.1(op)21.2(la)12.8(st)17.7(ic)]TJ 0 -1.0471 TD [(me)17.7(so)14.4(th)18.9(el)18.4(iu)18.8(m)-254.3(a)0(n)15.1(d)-245.5(pl)13.2(eu)20.8(ra)13.5(l)-247.8(m)0(e)12(t)13.9(as)13.9(ta)18.4(si)17.7(s)-252.3(w)0(e)16.9(r)0(e)-242.5(n)0(o)15.5(t)-247.8(po)15.5(si)17.7(ti)16.5(ve)-240.9(wi)14.9(th)]TJ 0 -1.0528 TD [(th)18.9(is)-397.7(me)17.7(th)13.2(od)21.2(.)-404.4(T)0(h)17.1(e)-410.9(ma)17.7(li)16.5(gn)21.2(an)15.1(t)-407.2(m)0(e)17.7(s)0(o)20(t)0(h)18.9(e)0(l)12.8(i)0(o)18.9(m)0(a)17.7(s)-406(pr)13.9(es)19.6(um)18.1(ed)-400.3(to)]TJ 0 -1.0471 TD [(ha)15.1(rb)19.6(ou)15.5(r)-212.9(S)0(V)18.1(4)0(0)-212.1(D)12.4(NA)-128.9(we)16.9(re)-208.4(al)18.4(so)-207.6(fo)19.6(un)15.5(d)-217(t)0(o)-208.8(b)0(e)-206.8(i)0(m)15.7(m)0(u)18.1(n)0(o)15.5(r)0(e)19.2(a)0(c)20.4(t)0(i)16.5(v)0(e)]TJ 0 -1.0527 TD [(wi)14.9(th)-322.6(th)18.9(e)-336.9(m)0(o)18.1(n)0(o)15.5(c)0(l)18.4(o)0(n)21.2(a)0(l)-328.7(a)0(n)20.8(t)0(i)16.5(b)0(o)15.5(d)0(y)-320.2(d)0(e)15.1(t)0(e)18.4(c)0(t)18.4(i)0(n)13.2(g)-330.8(th)18.9(e)-336.9(v)0(i)18.9(r)0(a)19.2(l)-333.2(sm)11.2(al)18.4(l)-333.2(t)0(-)]TJ 0 -1.0471 TD [(an)15.1(ti)22.2(ge)15.1(n)-279.6(o)0(f)-270.6(S)0(V)12.4(4)0(0)21.2(.)-279.2(Th)17.1(is)-272.6(is)-272.6(an)-275.1(in)18.9(di)18.9(ca)14.7(ti)16.5(on)-269(th)18.9(at)-271.8(th)18.9(e)-285.7(v)0(i)18.9(r)0(a)19.2(l)-282(SV)18.1(40)]TJ 0 -1.0528 TD [(DN)13.4(A)-283.5(i)0(s)-272.6(b)0(e)15.1(i)0(n)18.8(g)-285.3(tr)17.3(an)20.8(sl)12(at)18.5(ed)-275.1(to)-271.4(vi)13.2(ra)19.2(l)-282(o)0(n)15.5(c)0(o)15.1(p)0(r)19.6(o)0(t)18.9(e)0(i)18.4(n)-285.3(in)-277.1(ma)17.7(li)16.5(gn)21.2(an)15.1(t)]TJ 0 -1.0471 TD [(me)17.7(so)14.4(th)18.9(el)18.4(io)18.8(ma)12(.)-467(B)0(o)16.5(t)0(h)-459.2(n)0(u)15.5(c)0(l)18.4(e)0(a)14.7(r)-469(an)15.1(d)-467.4(c)0(y)15.1(t)0(o)18.9(p)0(l)18.9(a)0(s)19.6(m)0(i)15.7(c)-473.5(im)15.7(mu)18.1(no)15.5(r)20.4(-)]TJ 0 -1.0528 TD [(ea)14.7(ct)18.4(iv)18.9(it)16.5(y)-410.5(w)0(e)16.9(r)0(e)-407.6(f)0(o)19.6(u)0(n)15.5(d)-410.5(wi)14.9(th)-402.3(th)18.9(e)-410.9(m)0(o)12.4(n)0(o)21.2(c)0(l)18.4(o)0(n)15.5(a)0(l)-402.7(a)0(n)15.1(t)0(i)16.5(b)0(o)21.2(d)0(y)-405.6(P)11.4(ab)]TJ 0 -1.0471 TD [(28)15.5(0.)-194.6(T)12.2(h)0(i)18.9(s)-206.8(is)-192.9(no)15.5(t)-202.3(s)0(u)14.3(r)0(p)19.6(r)0(i)17.3(s)0(i)17.7(n)0(g)15.5(,)-199.6(as)-196.6(th)18.9(e)-206(m)0(o)18.1(n)0(o)15.5(c)0(l)18.4(o)0(n)21.2(a)0(l)-197.8(a)0(n)15.1(t)13.9(ib)13.2(od)21.2(y)-205.6(P)11.4(ab)]TJ 0 -1.0528 TD [(28)15.5(0,)-291.4(sp)20(ec)14.7(if)17.3(ic)18.4(al)18.4(ly)-294.1(de)15.1(te)18.4(ct)18.4(in)13.2(g)-302.4(t)0(h)18.9(e)-302.8(sm)16.9(al)18.4(l)-304.7(t)0(-)17.3(a)0(n)15.1(t)0(i)16.5(g)0(e)20.8(n)0(,)-297.1(i)0(s)-295.3(f)0(o)19.6(u)0(n)15.5(d)-302.4(to)]TJ 25.1977 43.7432 TD [(re)13.5(ac)20.4(t)-282(s)0(t)17.7(r)0(o)19.6(n)0(g)15.5(l)0(y)-271.4(w)12.4(it)16.5(h)-279.6(b)0(o)15.5(t)0(h)-265.7(t)0(h)13.2(e)-274.3(nu)15.5(cl)18.5(ea)14.7(r)-275.5(a)0(n)15.1(d)-279.6(cy)20.8(to)13.2(pl)18.9(as)19.6(mi)15.7(c)-280(f)0(o)19.6(r)0(m)]TJ 0 -1.0471 TD [(of)-219.4(th)18.9(e)-228.8(s)0(m)16.9(a)0(l)18.4(l)-225.1(t-)17.3(an)15.1(t)13.9(i)0(g)13.2(e)0(n)-212.5(t)0(h)13.2(a)0(t)-214.9(a)0(p)15.1(p)0(e)20.8(a)0(r)19.2(s)-229.6(to)-214.5(be)-218.2(as)19.6(so)14.4(ci)18.5(at)18.4(ed)-218.2(wi)20.6(th)-220.2(th)18.9(e)]TJ 0 -1.0528 TD [(cy)15.1(to)18.8(sk)14.4(el)18.5(et)18.5(on)-308.8(t)13.9(h)0(e)15.1(r)0(e)19.2(b)0(y)-308.8(e)0(x)15.1(p)0(l)18.9(a)0(i)18.4(n)0(i)18.9(n)0(g)-308.8(t)0(h)18.8(e)-314.2(ob)15.5(se)19.6(rv)19.6(ed)-309.2(nu)15.5(cl)18.5(ea)20.4(r)-315.3(a)0(n)15.1(d)]TJ T* [(cy)15.1(to)18.8(pl)18.9(as)13.9(mi)15.7(c)-319.8(i)0(m)15.7(m)0(u)12.4(n)0(o)21.2(r)0(e)13.5(a)0(c)20.4(t)0(i)16.5(v)0(i)18.9(t)0(y)-316.9(i)0(n)-316.9(t)0(h)18.9(e)-325.5(pr)19.6(es)19.6(en)15.1(t)-321.8(s)0(e)14(r)0(i)17.3(e)0(s)-316.1([)0(1)19.6(0].)]TJ 1.0015 -1.0471 TD [(Ho)11.6(w)12.4(e)0(v)15.1(e)0(r)47.7(,)-341.8(cy)20.8(to)18.9(pl)13.2(as)19.6(mi)15.7(c)-342.6(i)13.9(mm)14.9(un)15.5(or)19.6(ea)14.7(ct)18.4(iv)18.8(it)16.5(y)-342.2(h)0(a)20.8(s)-343.4(al)18.5(so)-332.8(be)15.1(en)]TJ -1.0015 -1.0527 TD [(de)15.1(sc)19.6(ri)17.3(be)15.1(d)-404.8(f)0(o)14(r)-406.4(ot)18.9(he)20.8(r)-412.1(c)0(l)18.4(a)0(s)19.6(s)0(i)17.7(c)0(a)14.7(l)-407.2(nu)21.2(cl)18.5(ea)14.7(r)-406.4(a)0(n)15.1(t)0(i)16.5(g)0(e)20.8(n)0(s)-401.1(s)0(u)20(c)0(h)-400.3(a)0(s)-395.8(c)0(-)]TJ 0 -1.0471 TD [(my)12.4(c,)-331.6(th)18.8(e)-342.6(c)0(e)14.7(l)0(l)16.5(u)0(l)18.9(a)0(r)-333.6(h)0(o)15.5(m)0(o)18.1(l)0(o)18.9(g)0(u)15.5(e)-342.6(of)-333.2(th)18.9(e)-342.6(v)0(-)13.9(m)0(y)18.1(c)-342.6(an)20.8(ti)16.5(ge)15.1(n.)-331.2(Fi)14(xa)20.8(-)]TJ 0 -1.0527 TD [(ti)16.5(on)-399.9(ti)16.5(me)17.7(s)-406(t)0(h)18.8(a)0(t)-402.7(a)0(r)19.2(e)-410.9(lo)18.9(ng)21.2(er)-401.9(th)18.9(an)-400.3(60)21.2(\2611)15.5(20)-399.9(mi)21.4(n)-410.5(s)0(e)19.6(e)0(m)-403.4(t)0(o)-396.5(i)0(n)18.8(-)]TJ 0 -1.0471 TD [(fl)17.3(ue)15.1(nc)15.1(e)-245.9(t)0(h)18.9(e)-245.9(ce)14.7(ll)16.5(ul)18.9(ar)-236.8(lo)13.2(ca)20.4(li)16.5(za)14.7(t)13.9(i)0(o)13.2(n)-245.5(of)-236.4(nu)15.5(cl)18.5(ea)14.7(r)-247.1(p)0(r)19.6(o)0(t)18.9(e)0(i)12.8(n)0(s)-236(s)0(u)20(c)0(h)-240.9(a)0(s)]TJ 0 -1.0527 TD [(c-)13.5(my)18.1(e)-439.3(a)0(n)20.8(d)-438.9(re)19.2(su)14.4(lt)-427.4(in)-430.7(cy)20.8(to)18.9(pl)13.2(as)19.6(mi)15.7(c)-439.3(i)0(m)15.7(m)0(u)18.1(n)0(o)21.2(r)0(e)13.5(a)0(c)20.4(t)0(i)16.5(v)0(i)18.9(t)0(y)-430.7([)0(1)48.1(1].)]TJ 0 -1.0471 TD [(Thi)-8.8(s)-485.7(phenom)-8.1(enon)-487.2(has)-487.6(also)-490.7(been)-493.3(described)-491.3(in)-493.3(paraf)9.3(fin)]TJ 0 -1.0528 TD [(embed)-7.8(ded)-332.8(form)-9.4(alin)-332.6(fixed)-338.3(tissue)-336.5(of)-333.2(human)-337.4(pleur)-8.2(al)-334.4(meso-)]TJ 0 -1.0471 TD [(thel)-8.2(ioma)-339(for)-335.5(c-fos)-338.7(and)-338.4(c-mye)-339.4(nuclea)-8.2(r)-332.4(anti)-8.2(gens)-334.7([12].)]TJ 1.0015 -1.0527 TD [(Sm)13.2(al)18.5(l)-378.7(t)0(-)17.3(a)0(n)15.1(t)0(i)16.5(g)0(e)20.8(n)-382(mi)15.7(gh)15.5(t)-378.7(p)0(l)18.9(a)0(y)-371.8(a)-382.4(ke)20.8(y)-382(r)0(o)19.6(l)0(e)-374.2(i)0(n)-373.8(t)0(h)18.9(e)-382.4(de)20.8(ve)15.1(lo)18.9(p-)]TJ -1.0015 -1.0471 TD [(me)12(nt)-328.3(of)-333.2(me)17.7(so)14.4(t)13.9(h)0(e)15.1(l)0(i)16.5(o)0(m)18.1(a)-342.6(as)-327.5(sm)11.2(al)18.5(l)-338.9(t)0(-)17.3(a)0(n)15.1(t)0(i)22.2(g)0(e)15.1(n)-342.2(mu)18.1(ta)18.5(nt)18.9(s)-343.4(a)0(r)19.2(e)-342.6(no)21.2(t)]TJ 0 -1.0527 TD [(ab)15.1(le)-323(to)-322.6(in)18.9(du)15.5(ce)-321(me)17.7(so)14.3(th)18.8(el)18.4(io)18.9(ma)-323.7(wh)17.3(en)-326.3(i)13.9(n)0(j)13.2(e)0(c)20.4(t)0(e)18.4(d)-330.8(in)13.2(tr)17.3(ap)20.8(le)18.4(ur)13.9(al)18.4(ly)]TJ T* [(in)-351(ha)20.8(ms)16.9(te)12.8(rs)-345.7(in)-345.3(co)15.1(nt)18.9(ra)13.5(st)-346.5(to)-345.3(wi)14.9(ld)-345.3(ty)13.2(pe)-343.4(SV)18.1(40)-348.7([1)13.9(3].)-356.3(Smal)-8.2(l)-361.7(t)0(-)]TJ 0 -1.0471 TD [(anti)-8.2(gen)-275.8(enhances)-282.1(the)-278.2(trans)-9.4(forming)-281.5(capaci)-8.6(ties)-276.9(of)-276.3(SV40)-279.3(by)]TJ 0 -1.0527 TD [(incre)-8.6(asing)-286.6(the)-283.9(product)-9.7(ion)-283.5(of)-282(t-ant)-10.6(igen:)-287.8(by)-280.4(contr)-8.2(ibuting)-285.6(to)]TJ 0 -1.0471 TD [(the)-283.9(complete)-282.7(inact)-9.4(ivation)-280.3(of)-276.3(cell)-8.6(ular)-280.6(p53,)-275.8(sti)-8.2(mulating)-284.2(mi-)]TJ 0 -1.0527 TD 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[(assays)-418.7(for)-409.5(detection)-413.6(of)-412.8(SV40)-410.2(virus)-413(must)-413.8(be)-406(inte)-8.2(rpreted)]TJ 0 -1.0527 TD [(wi)-7.8(th)-419.3(care)-421.3(as)-418.5(some)-423.2(PCR)-420.7(prime)-11.3(rs)-414(seem)-423.6(to)-419.3(ampl)-9(ify)-421.7(what)]TJ 0 -1.0471 TD [(app)-8.4(ears)-376.5(to)-379.5(be)-383.2(host)-376.5(sequenc)-7.7(es.)-384.8(Unfortu)-9.2(nately)-380(these)-383.8(PCR)]TJ 0 -1.0527 TD [(resul)-9.4(ts)-540(wer)-8.2(e)-541.8(not)-539.6(co)-7.6(nfirmed)-549.4(by)-542.1(positive)-549.8(immunoh)-8.3(isto-)]TJ T* [(che)-8.8(mistry)58.7(.)-262.1(I)0(t)-255.9(i)0(s)-261.2(unclea)-8.2(r)-258.4(i)0(n)-260(this)-259.4(study)-263.5(whether)-264.4(the)-261.1(presen)-8.2(ce)]TJ 0 -1.0471 TD [(of)-373(the)-369.3(SV40)-370.4(or)-367.3(SV40-)-8.5(like)-366.7(DN)-9.4(A)-363.2(sequenc)-7.7(es)-373(by)-365.7(the)-369.3(PCR)]TJ ET q 228.2595 0 0 161.8015 58.2803 617.329 cm /Im2 Do Q BT 7.97 0 0 7.97 58.28 605.764 Tm 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9.963 58.28 771.647 Tm 0 g /GS2 gs 0 Tc [(assay)-291.5(is)-289.6(just)-287.8(fort)-8.6(uitous)-289.4(as)-293.3(both)-290(neoplasti)-10.3(c)-285.7(tissue)-291(and)-292.9(non-)]TJ 0 -1.0471 TD [(neopla)-7.8(stic)-481.7(tis)-8.2(sue)-481.9(was)-485.8(positive)-487.2(for)-483.5(SV40)-484.2(or)-481.1(SV40)-484.2(like)]TJ 0 -1.0528 TD [(DNA)-8.4(.)-279.2(The)-285.7(major)-290.2(advant)-7.8(age)-281.9(of)-282(the)-289.6(PRINS)-285.6(met)-8.2(hod)-281.1(is)-283.9(that)]TJ T* [(it)-239.6(co)-7.6(mbines)-245(morp)-7.8(hology)-240.3(wi)-7.8(th)-237.2(mol)-7.8(ecular)-248.8(informat)-11.9(ion.)-238.4(Not)]TJ 0 -1.0471 TD [(only)-329.8(does)-329(it)-330.7(detect,)-329.6(but)-329(also)-331.4(visua)-7.8(lizes)-329.2(the)-329.4(DNA)-332.8(there)-8.6(by)]TJ 0 -1.0527 TD [(perm)-8.2(itting)-378.3(its)-378.1(localizat)-12.3(ion)-374.6(in)-373.8(a)-371.1(speci)-8.2(fic)-376.6(cell)-8.6(.)-370.3(I)0(n)-373(con)-8.4(trast,)]TJ 0 -1.0471 TD [(classical)-331(PCR)-323.9(o)-6.5(nly)-323.3(gives)-326.5(us)-321.4(an)-320.6(overal)-9.4(l)-321.8(impressio)-13.5(n)-319.4(about)]TJ 0 -1.0527 TD [(the)-397.7(SV40)-404.5(DNA)-401(conten)-7.8(t)-395.8(o)0(f)-401.5(a)-393.8(given)-404.9(tissue)-404.8(and)-395.3(does)-403(not)]TJ 0 -1.0471 TD [(perm)-8.2(it)-279.4(the)-283.9(localizat)-12.3(ion)-283.5(of)-276.3(the)-283.9(viral)-289.4(DNA.)-281.9(If)-272.2(fals)-8.6(e)-280(positive)]TJ 0 -1.0527 TD [(results)-435.6(occur)22.2(,)-427.2(i)0(t)-433.1(i)0(s)-431.9(more)-435(likely)-433.2(that)-435(these)-435(will)-435.2(be)-428.7(more)]TJ 0 -1.0471 TD [(readi)-8.6(ly)-430.7(apparen)-8.1(t)-429.9(w)0(i)-7.8(t)0(h)-430.7(a)-433.7(morpholog)-8.3(ical)-435.4(meth)-9(od)-428.3(such)-437.1(as)]TJ 0 -1.0528 TD [(PRINS)-342.5(than)-335.9(with)-336.1(c)-6.9(lassical)-341.2(PCR.)]TJ 1.0015 -1.0471 TD [(In)-225.1(ad)20.8(di)18.9(ti)16.5(on)-223.5(to)-220.2(tu)18.9(mo)12.4(ur)19.6(s,)-224.3(S)11.4(V)0(4)17.3(0)-234.1(D)12.4(N)0(A)-225.6(h)0(a)15.1(s)-229.6(be)20.8(en)-223.9(de)15.1(te)18.4(ct)18.4(ed)-223.9(in)]TJ -1.0015 -1.0527 TD [(hu)15.5(ma)17.7(n)-239.8(p)0(i)18.9(t)0(u)18.9(i)0(t)16.5(a)0(r)13.5(y)-239.8(gl)18.9(an)20.8(d,)-234.5(pe)20.8(ri)17.3(ph)15.5(er)19.2(al)-231.9(mo)12.4(no)21.2(nu)15.5(cl)18.4(ea)14.7(r)-241.4(b)0(l)18.9(o)0(o)21.2(d)-239.8(ce)14.7(ll)16.5(s)]TJ 0 -1.0471 TD [(an)15.1(d)-524.3(s)0(e)19.6(m)0(i)15.7(n)0(a)15.1(l)-526.7(fl)17.3(ui)18.9(ds)-514.9(fr)12.4(om)-516.8(he)20.8(al)12.8(th)18.9(y)-524.3(s)0(u)14.4(b)0(j)18.9(e)0(c)20.4(t)0(s)-517.2([)0(2)13.9(2)0(])-526.7(there)-8.6(by)]TJ 0 -1.0527 TD [(explai)-10.1(ning)-318.4(the)-323.7(resul)-9.4(ts)-318.1(in)-322.6(the)-323.7(study)-326.1(of)-321.8(G)]TJ 6.974 0 0 6.974 216.737 614.721 Tm 0.0001 Tc [(ALA)97.3(TEAU)]TJ 9.963 0 0 9.963 248.768 614.721 Tm 0 Tc (-S)Tj 6.974 0 0 6.974 257.499 614.721 Tm 0.0001 Tc (ALLE)Tj /F4 1 Tf 9.963 0 0 9.963 278.362 614.721 Tm 0 Tc (et)Tj -22.0907 -1.0527 TD (al.)Tj /F3 1 Tf 1.2861 0 TD [([21].)-268.4(The)-268.6(transmi)-10.7(ssion)-270.3(of)-264.9(SV40)-268(virus)-270.7(is)-266.9(not)-266.4(very)-266.8(clear)]TJ -1.2861 -1.0471 TD [(at)-397(present.)-398.7(Some)-398.5(studies)-399.8(suggest)-397.5(that)-395.2(SV40)-398.8(might)-398.7(be)-394.6(a)]TJ 0 -1.0527 TD [(contam)-10.9(inant)-413(of)-412.8(polio)-412.6(vaccin)-8.2(es)-412.8(adminis)-9.1(terd)-411.5(in)-413.6(the)-414.8(years)]TJ 0 -1.0471 TD [(1950\261196)-6.1(0.)-342.6(The)-342.6(exact)-348.8(nature)-345.1(of)-344.6(the)-340.8(SV40)-347.6(or)-344.6(SV40-li)-9.1(ke)]TJ 0 -1.0527 TD [(DNA)-378.3(reveal)-9.8(ed)-371.8(by)-377.1(PCR)-380.8(assays)-378.8(is)-375(still)-378.7(an)-377.5(enigma.)-381.2(Some)]TJ 0 -1.0471 TD [(primer)-224.2(sets)-213.1(resul)-9.4(t)-213.7(i)0(n)-214.5(a)-217.4(higher)-219.5(percentage)-224.2(of)-213.7(posit)-9.8(ive)-215.6(results)]TJ 0 -1.0527 TD [(than)-398.5(others)-397.1([4].)-398.5(The)-393.8(primers)-402.6(speci)-8.2(fic)-393.6(for)-398.1(the)-397.7(Rb-pocket)]TJ 0 -1.0471 TD [(give)-250.5(the)-255.5(highes)-8.6(t)-247.8(positive)-259.5(results.)-253.9(Preli)-9.8(minary)-256.8(data)-250.9(sugges)-7.4(t)]TJ 0 -1.0527 TD [(that)-361(prim)-10.2(ers)-358.3(consi)-7.8(dered)-359(speci)-8.2(fic)-359.5(for)-364(SV40)-364.7(would,)-363.3(under)]TJ 0 -1.0471 TD [(certa)-9(in)-538.8(condition)-8.7(s,)-537.2(ampl)-9(ify)-541.2(what)-544.7(appeared)-544.2(to)-544.5(be)-536.9(host)]TJ 0 -1.0528 TD [(DNA)-332.8(sequenc)-7.7(es,)-333.6(indicati)-10.7(ng)-325.9(that)-332.6(PCR)-335.3(based)-330.2(assays)-333.3(must)]TJ T* [(be)-218.2(interpret)-12.3(ed)-212.5(wi)-7.8(th)-214.5(caution)-221.5(and)-218.9(correlated)-227.7(with)-216.6(expres)-8.2(sion)]TJ 0 -1.0471 TD [(of)-270.6(viral)-278(T)86.2(-prot)-9.4(eins)-274.5([18].)-274.1(SV40)-273.7(might)-273.5(be)-269.4(an)-269.4(en)-7.6(demic)-271.9(virus)]TJ 0 -1.0528 TD [(in)-362.4(the)-363.6(human)-365.9(popula)-7.4(tion)-360.6(and)-366.9(be)-360.4(occasi)-9.4(onally)-362.6(presen)-8.2(t)-361.7(i)0(n)]TJ 0 -1.0471 TD [(human)-337.4(tis)-8.2(sues)-335.9(in)-334(low)-338.7(copy)-339.2(numbers.)]TJ 1.0015 -1.0528 TD [(Su)16.3(ch)-417.3(en)20.8(de)15.1(mi)15.7(c)-422.3(v)0(i)13.2(r)0(u)19.6(s)0(e)19.6(s)-428.7(ma)17.7(y)-427.6(a)0(c)20.4(c)0(u)15.1(m)0(u)18.1(l)0(a)18.4(t)0(e)-419.7(v)0(a)20.8(r)0(i)17.3(o)0(u)15.5(s)-423.1(mu)12.4(ta)18.4(-)]TJ -1.0015 -1.0471 TD [(ti)16.5(on)21.2(s)-332(a)0(n)15.1(d)-325.1(de)15.1(le)18.4(ti)16.5(on)15.5(s.)-315.3(Mu)13.6(ta)18.4(ti)16.5(on)21.2(s)-332(a)0(n)15.1(d)-325.1(de)15.1(le)18.4(ti)16.5(on)15.5(s)-326.3(o)0(f)-321.8(t)0(h)18.8(e)-331.2(SV)18.1(40)]TJ 0 -1.0527 TD [(ge)15.1(no)21.2(me)-380.7(ma)17.7(y)-387.7(a)0(l)18.4(s)0(o)-378.3(o)0(c)15.1(c)0(u)15.1(r)-383.6(in)-379.5(hu)15.5(ma)17.7(n)-387.7(t)0(u)18.9(m)0(o)18.1(u)0(r)13.9(s)-388.9(bu)21.2(t)-390.1(a)0(r)19.2(e)-388.1(ve)15.1(ry)]TJ 0 -1.0471 TD [(un)15.5(li)22.2(ke)15.1(ly)-356.7(to)-356.7(oc)15.1(cu)20.8(r)-372.2(i)13.9(n)-370.7(t)0(h)18.9(e)-365.4(Rb)-359.1(po)15.5(ck)20.8(et)-357.1(bi)13.2(nd)21.2(in)18.9(g)-370.7(d)0(o)21.2(m)0(a)17.7(i)0(n)-356.7(o)0(f)-361.6(t)0(-)]TJ 0 -1.0527 TD [(an)15.1(ti)22.2(ge)15.1(n)-478.8(b)0(e)20.8(c)0(a)14.7(u)0(s)20(e)-479.2(of)-469.7(th)18.9(e)-479.2(r)0(e)19.2(l)0(e)12.8(v)0(a)20.8(n)0(c)15.1(e)-473.5(of)-469.7(th)13.2(i)13.9(s)-480(re)19.2(gi)13.2(on)-468.2(in)-464.8(ce)14.7(ll)]TJ 0 -1.0471 TD [(tr)17.3(an)15.1(sf)18.4(or)19.6(ma)17.7(ti)16.5(on)-434([2)19.6(3,)-445(24].)-453.8(It)-449.4(is)-454.7(also)-450.9(possi)-8.6(ble)-454.6(that)-452.1(the)-454.6(se-)]TJ 0 -1.0528 TD [(quence)-495.2(detect)-10.6(ed)-491.3(by)-490.9(these)-491.9(sets)-491.9(o)-6.5(f)-486.1(prim)-10.2(ers)-489.2(belong)-496.7(to)-493.3(a)]TJ T* [(recom)-9.4(binant)-317(betw)-9.8(een)-316.1(SV40)-319.2(and)-315.7(anothe)-7.8(r)-315.3(unknow)-8.5(n)-313.8(virus)-9(.)]TJ 0 -1.0471 TD [(This)-403.4(hypoth)-7(esis)-400.9(is)-397.7(corrob)-8.2(orate)-8.6(d)-399.1(b)0(y)-399.9(the)-397.7(fact)-406.2(that)-400.9(human)]TJ 0 -1.0527 TD [(speci)-8.2(mens)-337.8(whi)-8.6(ch)-337.7(contain)-341(SV40)-341.9(are)-340.4(also)-342.8(coinf)-8.2(ected)-343.2(with)]TJ 0 -1.0471 TD [(the)-340.8(BK)-345.9(virus.)-345.1(Furthe)-8.2(rmore,)-346.7(the)-340.8(aden)-9.6(ovirus)-345.5(3)-336.5(and)-344.1(7)-342.2(vac-)]TJ 0 -1.0527 TD [(cines)-241.5(used)-238(between)-240.5(1961)-236.4(and)-236(1965)-242.1(contained)-240.5(adenovi)-8.5(rus-)]TJ 0 -1.0471 TD [(SV40)-279.3(hybri)-7.8(ds)-270.2(wi)-7.8(th)-271.4(segm)-7.8(ents)-280.2(of)-276.3(the)-278.2(SV40)-273.7(gen)-8.4(ome)-273.3(whi)-8.6(ch)]TJ 0 -1.0528 TD [(includ)-9.7(ed)-394.6(the)-392(SV40)-398.8(t-ant)-10.6(igen)-392.8(e)-6.9(nclosed)-396.7(in)-396.6(their)-397.5(recom)-9.4(bi-)]TJ 0 -1.0471 TD [(nant)-335.9(genom)-7.4(e)-336.9([4].)]TJ 1.0015 -1.0528 TD [(Th)17.1(es)19.6(e)-348.3(f)0(i)17.3(n)0(d)15.5(i)0(n)18.9(g)0(s)-338.5(i)0(n)13.2(d)0(i)18.9(c)0(a)20.4(t)0(e)-340.1(t)0(h)13.2(a)0(t)-340.1(s)0(o)20(m)0(e)-340.8(S)11.4(V4)11.6(0)-347.9(s)0(e)19.6(q)0(u)15.5(e)0(n)20.8(c)0(e)14.7(s)-349.1(i)13.9(n)0(-)]TJ -1.0015 -1.0471 TD [(cl)18.4(ud)15.5(in)18.9(g)-342.2(o)0(n)15.5(c)0(o)20.8(g)0(e)15.1(n)0(i)18.9(c)-342.6(se)13.9(qu)21.2(en)15.1(ce)20.4(s)-349.1(c)0(o)20.8(d)0(i)18.9(n)0(g)-337.3(f)0(o)19.6(r)-343.8(t-)17.3(an)15.1(ti)16.5(ge)20.8(n)-347.9(c)0(a)20.4(n)-342.2(be)]TJ 0 -1.0528 TD [(tr)17.3(an)15.1(sf)18.4(er)19.2(re)13.5(d)-393.4(a)0(n)15.1(d)-393.4(in)13.2(co)20.8(rp)13.9(or)19.6(at)18.4(ed)-388.9(in)18.9(to)-390.9(th)18.9(e)-399.5(g)0(e)20.8(n)0(o)15.5(m)0(e)-392(o)0(f)-384.4(a)0(n)15.1(o)0(t)18.9(h)0(e)15.1(r)]TJ T* [(vi)18.9(ru)13.9(s.)-212.9(T)12.2(h)0(e)-218.2(l)0(a)18.4(t)0(t)16.5(e)0(r)-214.1(v)0(i)18.9(r)0(u)13.9(s)-218.2(ac)14.7(ti)16.5(ng)-212.1(as)-213.7(a)-223.1(c)0(a)14.7(r)0(r)18(i)0(e)18.4(r)-224.3(is)-215.6(tr)17.3(an)15.1(sf)18.4(or)19.6(me)17.7(d)-222.7(i)0(n)13.2(t)0(o)]TJ 0 -1.0471 TD [(an)-297.8(on)21.2(co)15.1(ge)20.8(ni)13.2(c)-302.8(v)0(i)18.9(r)0(u)13.9(s)0(.)-292.6(I)0(n)-299(t)0(h)18.9(i)0(s)-295.3(w)0(a)16.9(y)-308.1(")13.4(u)0(n)15.5(h)0(a)20.8(r)0(m)10.8(f)0(u)19.6(l)0(")-297.1(v)0(i)18.9(r)0(u)19.6(s)0(e)13.9(s)-303.6(su)20(ch)]TJ 0 -1.0528 TD [(as)-247.8(ad)15.1(en)20.8(ov)15.5(ir)17.3(us)20(es)-253.5(se)19.6(rv)19.6(in)13.2(g)-256.8(a)0(s)-247.8(a)-257.2(ca)14.7(rr)18(ie)18.4(r)-258.4(m)0(a)17.7(y)-262.5(sp)20(re)19.2(ad)-252.3(on)21.2(co)15.1(ge)20.8(ni)13.2(c)]TJ 0 -1.0471 TD [(DN)13.4(A)-323.4(s)0(e)19.6(q)0(u)15.5(e)0(n)20.8(c)0(e)14.7(s)-326.3(in)-311.2(th)13.2(e)-319.8(h)0(u)15.5(m)0(a)17.7(n)-325.1(po)21.2(pu)15.5(la)18.4(ti)16.5(on)15.5(.)]TJ 1.0015 -1.0528 TD [(It)-267.3(is)-266.9(co)15.1(nc)15.1(l)13.9(u)0(d)15.5(e)0(d)-269.4(t)0(h)18.9(a)0(t)-266.1(t)0(h)18.9(e)-280(pr)19.6(im)15.7(ed)]TJ /F4 1 Tf 12.2973 0 TD [(in)-265.7(si)17.7(tu)]TJ /F3 1 Tf 2.7144 0 TD [(m)13.2(e)0(t)12.8(h)0(o)21.2(d)-279.6(is)-266.9(ab)20.8(le)-271.8(to)]TJ -16.0132 -1.0471 TD [(de)15.1(te)18.4(ct)-345.7(si)17.7(mi)15.7(an)-349.1(va)15.1(cu)20.8(ol)18.9(at)12.8(in)18.9(g)-353.6(v)0(i)13.2(r)0(u)19.6(s)-354.8(40)-348.7(or)-350.2(si)17.7(mi)15.7(an)-349.1(va)20.8(cu)15.1(ol)18.9(at)18.4(in)13.2(g)]TJ 0 -1.0527 TD [(vi)18.9(ru)13.9(s)-525.5(4)0(0)15.5(-)0(l)17.3(i)0(k)18.9(e)-530.4(de)20.8(ox)15.5(yr)19.6(ib)18.9(on)15.5(uc)15.1(l)13.9(e)0(i)12.8(c)-524.7(ac)14.7(id)-521.7(in)-516.1(th)13.2(e)-524.7(m)0(a)12(j)0(o)18.9(r)0(i)17.3(t)0(y)-521.8(o)0(f)]TJ 0 -1.0471 TD [(me)17.7(so)14.4(th)18.9(el)18.4(io)18.8(ma)-397.7(in)-396.6(co)15.1(nt)18.9(ra)19.2(st)-397.7(to)-396.6(no)15.5(n-)19.6(ne)15.1(op)21.2(la)12.8(st)17.7(ic)-397(me)17.7(so)20(th)13.2(el)18.4(iu)18.8(m)]TJ 0 -1.0527 TD [(an)15.1(d)-245.5(p)0(l)18.9(e)0(u)15.1(r)0(a)19.2(l)-247.8(ca)20.4(rc)13.5(in)18.9(om)18.1(a)-245.9(m)0(e)12(s)0(t)17.7(a)0(s)19.6(t)0(a)18.4(s)0(i)17.7(s)-246.6(th)13.2(at)-237.6(w)12.4(e)0(r)13.5(e)-245.9(si)17.7(mi)15.7(an)-235.2(va)15.1(cu)15.1(o-)]TJ 0 -1.0471 TD [(la)18.4(ti)16.5(ng)-325.9(vi)18.9(ru)19.6(s)-332(4)0(0)-325.9(d)0(e)20.8(o)0(x)15.5(y)0(r)19.6(i)0(b)18.9(o)0(n)15.5(u)0(c)20.8(l)0(e)12.8(i)13.9(c)-336.9(a)0(c)20.4(i)0(d)18.9(-)-332.4(an)15.1(d)-330.8(s)0(i)17.7(m)0(i)15.7(a)0(n)-320.6(v)0(a)15.1(c)0(u)15.1(o)0(-)]TJ 0 -1.0527 TD [(la)18.4(ti)16.5(ng)-399.9(vi)18.9(ru)19.6(s)-406(4)0(0)-399.9(p)0(r)19.6(o)0(t)18.9(e)0(i)18.4(n)0(-)14(n)0(e)20.8(g)0(a)15.1(t)0(i)16.5(v)0(e)20.8(.)-404.4(Th)17.1(e)-405.2(s)0(i)12(m)13.2(ia)12.8(n)-404.8(v)0(a)20.8(c)0(u)15.1(o)0(l)18.9(a)0(t)18.4(i)0(n)13.2(g)]TJ 0 -1.0471 TD [(vi)18.9(ru)13.9(s)-269.4(4)0(0)-269(d)0(e)20.8(o)0(x)15.5(y)0(r)19.6(i)0(b)18.9(o)0(n)15.5(u)0(c)20.8(l)0(e)12.8(i)13.9(c)-274.3(a)0(c)14.7(i)0(d)-265.7(s)0(e)19.6(e)0(m)17.7(s)-275.1(to)-265.7(be)-263.7(tr)17.3(an)15.1(sl)17.7(at)18.4(io)18.8(na)15.1(ll)16.5(y)]TJ 0 -1.0527 TD [(ac)14.7(t)13.9(i)0(v)13.2(e)-484.9(si)17.7(nc)15.1(e)-484.9(a)0(l)18.5(l)-486.8(th)18.9(e)-490.6(p)0(o)21.2(s)0(i)17.7(t)0(i)16.5(v)0(e)-479.9(c)0(a)20.4(s)0(e)13.9(s)-485.7(we)16.9(re)-475.8(al)18.4(so)-480.7(im)15.7(mu)18.1(no)15.5(r)20.4(-)]TJ T* 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[(McMenamin)-348.1(M.)-344.5(Establishment)-345.3(of)-347.7(human)]TJ /F4 1 Tf 17.1098 0 TD [(in)-349.1(vitr)34.2(o)]TJ /F3 1 Tf 3.2942 0 TD (meso-)Tj -20.404 -1.1065 TD [(thelial)-252.1(cell)-248.9(model)-254.4(system)-250(for)-247(investigating)-252.6(mechanisms)-255.6(of)]TJ 0 -1.1128 TD [(asbestos)-304.9(induced)-308.2(mesothelioma.)]TJ /F4 1 Tf 13.158 0 TD [(Am)-303(J)-309.1(Pathol)]TJ /F3 1 Tf 5.3176 0 TD [(1989;)-303.3(134:)]TJ -18.4755 -1.1128 TD (979\261991.)Tj -2.5165 -1.1128 TD [(2.)-1766.5(Fanning)-401.8(E,)-397.9(Knipers)-408.7(R.)-399.1(Structure)-404.9(and)-402.9(function)-399.8(of)-404.6(SV40)]TJ 2.5165 -1.1065 TD [(lar)11(ge)-346.5(tumour)-343.6(antigen.)]TJ /F4 1 Tf 9.0165 0 TD [(Annu)-342.9(Rev)-343(Biochem)]TJ /F3 1 Tf 8.0744 0 TD [(1992;)-347.6(61:)-342.3(55\261)]TJ -17.0908 -1.1128 TD (85.)Tj -2.5165 -1.1128 TD [(3.)-1766.5(Soriano)-295.3(F)77.8(,)-293.8(Shelburne)-294.1(E,)-290.4(Gokcen)-297(M.)-287.6(Simian)-292.8(virus)-299.2(40)-289.9(in)-292.2(a)]TJ 2.5165 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