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[(Fi)19.6(br)13.9(eo)20.8(pt)13.2(ic)-653(br)19.6(on)15.5(ch)20.8(os)14.4(co)20.8(py)-655.9(wa)16.9(s)-662.1(u)0(n)15.5(d)0(e)15.1(r)0(t)17.3(a)0(k)20.8(e)0(n)-656.3(a)0(c)20.4(c)0(o)15.1(r)0(d)19.6(i)0(n)13.2(g)]TJ -1.0015 -1.0471 TD [(to)-590(pr)13.9(ev)20.8(io)18.9(us)14.4(ly)-590(pu)15.5(bl)18.9(is)17.7(he)20.8(d)-604(m)0(e)17.7(t)0(h)18.9(o)0(d)15.5(s)-599.5([1)19.6(7].)-606.7(Biopsies)-615.9(wer)-8.2(e)]TJ 0 -1.0527 TD [(proces)-8.2(sed)-339.6(into)-337.9(glycol)-339.8(met)-8.2(hacrylate)-344.9(\(GMA)-8.8(\))-332.4(resi)-8.6(n)-336.5(for)-335.5(im-)]TJ 0 -1.0471 TD [(munoh)-7(istochem)-10.3(istry)56.9(,)-284.9(a)0(s)-287.7(previ)-8.2(ously)-291.9(described)-292.1([18].)-291.1(Semi)-8.2(-)]TJ 0 -1.0528 TD [(thin)-411.8(2-mm)-409.3(sections)-412.4(wer)-8.2(e)-405.2(cut)-409.1(and)-406.7(immunos)-9.5(tain)-8.2(ed)-406(using)]TJ 0 -1.0471 TD [(a)-462.1(streptavidine)-12.1(-biot)-10.2(in-peroxid)-10.3(ase)-459.5(detect)-10.6(ion)-459.9(system)-468.1(with)]TJ 0 -1.0528 TD [(amino)-9.7(ethylcarbaz)-11.1(ole)-392(as)-395.8(a)-393.8(chromogen)-399.4(which)-397.5(produce)-7.7(s)-394.6(a)]TJ T* [(red)-334.3(react)-9(ion)-334.7(product)-9.7(.)]TJ 1.0015 -1.0471 TD [(T)69.1(o)-461.7(in)18.9(ve)20.8(st)12(i)13.9(g)0(a)15.1(t)0(e)-453.9(t)0(h)18.9(e)-462.1(ex)15.1(pr)19.6(es)19.6(si)17.7(on)-456.8(of)-452.7(ad)20.8(he)15.1(si)17.7(on)-451.1(mo)12.4(l)13.9(e)0(c)14.7(u)0(l)18.9(e)0(s)13.9(,)]TJ -1.0015 -1.0527 TD [(se)19.6(ri)17.3(al)-271.8(se)19.6(ct)18.5(io)13.2(ns)-264.5(we)16.9(re)-271(st)17.7(ai)18.4(ne)15.1(d)-273.9(f)0(o)19.6(r)-281.2(IC)20.6(AM)15.3(-1)-270.6(\(C)14.9(D)12.4(5)0(4)15.5(;)-276.3(Ro)16.5(th)18.9(le)18.4(in)13.2(,)]TJ 0 -1.0471 TD [(Bo)16.5(eh)20.8(ri)11.6(ng)21.2(er)-430.3(In)19.6(ge)15.1(lh)18.9(ei)18.5(m,)-431.1(R)11.6(i)0(d)13.2(g)0(e)20.8(f)0(i)17.3(e)0(l)18.4(d)0(,)-427.9(C)0(T)86.4(,)-432.9(US)12.4(A)12.4(\))0(,)-429.5(V)0(C)12.6(A)12.4(M-)12(1)]TJ 0 -1.0528 TD [(\(C)14.9(D1)17.3(06)21.2(;)-242.1(S)11.4(er)13.5(ot)18.9(ec)14.7(,)-233.7(O)0(x)17.3(f)0(o)13.9(r)0(d)19.6(,)-239.4(Ox)17.3(fo)13.9(rd)19.6(sh)20(ir)17.3(e,)-234.9(U)12.4(K)0(\))15.7(,)-239.4(E-)-234.9(an)20.8(d)-239.8(P)11.4(-s)12.8(el)18.4(-)]TJ 0 -1.0471 TD [(ec)14.7(t)13.9(i)0(n)-493.3(\()0(C)14.9(D)12.4(62)15.5(E)-500(a)0(n)20.8(d)-501.5(C)11.6(D)0(6)17.3(2)0(P)16.3(;)-498.2(Co)16.5(ul)18.9(te)12.8(r)-497.4(E)12.2(le)12.8(ct)18.4(ro)19.6(ni)18.9(cs)13.9(,)-495.5(L)0(u)17.1(t)0(o)18.9(n)0(,)]TJ 0 -1.0528 TD [(Be)16.1(df)19.6(or)13.9(ds)20(hi)18.9(re)13.5(,)-239.4(U)0(K)19.1(\))-241.4(an)15.1(d)-239.8(t)0(h)18.9(e)-245.9(ve)20.8(ss)18.9(el)-231.9(ma)12(rk)19.6(er)-231.2(EN)13.2(-4)-230.7(\(B)14.9(ra)19.2(ds)14.4(ha)20.8(w)]TJ 0 -1.0471 TD [(Bi)14.1(ol)18.9(og)21.2(ic)12.8(al)18.5(s,)-434.8(L)12.2(o)0(u)15.5(g)0(h)21.2(b)0(o)15.5(r)0(o)19.6(u)0(g)15.5(h)0(,)-433.6(L)0(e)16.7(i)0(c)18.4(e)0(s)19.6(t)0(e)12.8(r)0(s)18.4(h)0(i)18.9(r)0(e)19.2(,)-444.2(UK)13.4(\).)-435.2(Mo)19.3(no)15.5(-)]TJ 0 -1.0528 TD [(cl)18.4(on)15.5(al)-345.7(an)20.8(ti)16.5(bo)15.5(di)18.9(es)-344.6(we)16.9(re)-350.7(al)18.5(so)-344.2(em)17.7(pl)18.9(oy)15.5(ed)-343.4(to)-345.3(id)13.2(en)20.8(ti)16.5(fy)-344.6(th)13.2(e)-354(l)0(i)16.5(g)0(-)]TJ 0 -1.0471 TD [(an)15.1(ds)-429.5(fo)19.6(r)-440.5(I)0(C)20.6(A)0(M)15.3(-)0(1)-429.9(a)0(n)15.1(d)-438.9(V)12.4(C)0(A)12.6(M)0(-)17.7(1)0(,)-427.9(t)0(h)18.9(e)]TJ /F7 1 Tf 15.5409 0 TD (b)Tj /F3 1 Tf 7.472 0 0 6.641 218.551 110.891 Tm (2)Tj 9.963 0 0 9.963 222.179 112.535 Tm [(-i)17.3(nt)18.9(eg)15.1(ri)17.3(n)-433.3(L)0(F)86.2(A)0(-)15.7(1)]TJ 8.7464 40.8012 TD [(\(C)14.9(D1)45.8(1a)20.8(\))-400.7(a)0(n)20.8(d)]TJ /F7 1 Tf 5.5938 0 TD (b)Tj /F3 1 Tf 0.5463 0 TD [(1-)19.6(in)13.2(t)13.9(e)0(g)15.1(r)0(i)17.3(n)-393.4(VL)13.2(A-)15.7(4)-393.4(\()0(C)14.9(D)0(4)17.3(9)0(d)15.5(;)-395.8(C)11.6(y)0(m)12.4(b)0(u)21.2(s)-394.6(Bi)14.1(o-)]TJ -6.1401 -1.0528 TD [(sc)13.9(ie)18.4(nc)15.1(e)-319.8(L)0(t)14.7(d)0(,)-314.1(S)11.4(ou)15.5(th)18.8(am)17.7(pt)13.2(on)21.2(,)-324.7(U)0(K)13.4(\))-321(re)13.5(sp)20(ec)20.4(ti)16.5(ve)15.1(ly)81.4(.)]TJ 1.0015 -1.0471 TD [(Al)14.9(l)-253.5(s)0(e)13.9(c)0(t)18.5(i)0(o)18.9(n)0(s)-241.7(f)0(o)13.9(r)-252.7(ea)20.4(ch)-246.6(an)20.8(ti)16.5(bo)15.5(dy)-240.5(we)11.2(re)-242.5(ex)20.8(am)12(in)18.9(ed)-246.6(si)17.7(mu)18.1(lt)16.5(an)20.8(-)]TJ -1.0015 -1.0527 TD [(eo)15.1(us)20(ly)-419.3(by)-422.6(a)-428(b)0(l)18.9(i)0(n)13.2(d)0(e)20.8(d)-427.6(in)13.2(ve)20.8(st)17.7(ig)18.9(at)12.8(or)-418.5(\(B)14.9(.)-427.2(V)63.6(ru)13.9(gt)18.9(\).)-418.2(On)17.3(ly)-425(po)21.2(si)17.7(-)]TJ 0 -1.0471 TD [(ti)16.5(ve)15.1(ly)-191.7(st)17.7(ai)18.4(ne)20.8(d)-199.9(v)0(e)15.1(s)0(s)18.9(e)0(l)18.5(s)-201.1(an)20.8(d)-199.9(n)0(u)15.5(c)0(l)18.4(e)0(a)20.4(t)0(e)12.8(d)0(,)-188.9(p)0(o)21.2(s)0(i)17.7(t)0(i)16.5(v)0(e)15.1(l)0(y)-186(s)0(t)17.7(a)0(i)12.8(n)0(e)20.8(d)-199.9(ce)20.4(ll)16.5(s)]TJ 0 -1.0528 TD [(we)16.9(re)-310.8(co)15.1(un)15.5(t)13.9(e)0(d)-314.9(a)0(t)]TJ /F9 1 Tf 6.6352 0 TD (3)Tj /F3 1 Tf 0.8251 0 TD [(40)-314.5(ma)17.7(gn)21.2(if)17.3(ic)12.8(at)18.5(io)18.9(n.)-308.4(Th)17.1(e)-319.8(a)0(r)19.2(e)0(a)-309.6(o)0(f)-310.4(t)0(h)13.2(e)-319.8(su)20(b-)]TJ -7.4603 -1.0527 TD [(mu)12.4(co)20.8(sa)-481.1(wa)16.9(s)-485.7(m)0(e)12(a)0(s)19.6(u)0(r)19.6(e)0(d)-479.9(u)0(s)14.4(i)0(n)18.9(g)-490.2(t)13.9(h)0(e)-479.9(C)0(o)16.5(l)0(o)18.8(u)0(r)14(v)0(i)18.9(s)0(i)17.7(o)0(n)-479.5(1)0(.)15.9(6)0(-)19.6(4)0(S)16.3(R)]TJ 0 -1.0471 TD [(so)14.4(ft)17.3(wa)16.9(re)-253.9(pa)20.8(ck)15.1(ag)20.8(e)-262.9(o)0(n)-251.9(a)-257.2(Ma)13.2(ci)18.4(nt)18.9(os)14.4(h)-256.8(c)0(o)15.1(m)0(p)18.1(u)0(t)18.9(e)0(r)13.5(i)13.9(ze)14.7(d)-262.5(i)0(m)21.4(a)0(g)15.1(e)-262.9(an)20.8(-)]TJ 0 -1.0527 TD [(al)12.8(ys)20(er)-299.4(\(I)12.4(m)13.2(p)0(r)13.9(o)0(v)21.2(i)0(s)17.7(i)0(o)13.2(n)0(,)-297.1(B)0(i)14.1(r)0(m)16.5(i)0(n)18.9(g)0(h)15.5(a)0(m)17.7(,)-307.7(UK)13.4(\))-309.7(e)0(x)20.8(c)0(l)18.5(u)0(d)15.5(i)0(n)18.8(g)-308.1(sm)11.2(oo)21.2(th)]TJ 0 -1.0471 TD [(mu)12.4(sc)19.6(le)-340.1(an)20.8(d)-347.9(g)0(l)18.9(a)0(n)20.8(d)0(u)15.5(l)0(a)18.4(r)-343.8(ti)16.5(ss)13.2(ue)20.8(.)-347.5(T)12.2(he)-337.7(to)18.9(ta)12.8(l)-344.6(c)0(o)20.8(u)0(n)15.5(t)-344.6(of)-338.9(ce)20.4(ll)16.5(s)-343.4(a)0(n)15.1(d)]TJ 0 -1.0527 TD [(th)13.2(e)-268.6(d)0(e)20.8(n)0(s)20(i)0(t)16.5(y)-268.2(of)-259.2(ve)15.1(ss)18.8(el)18.4(s)-269.4(w)0(e)16.9(r)0(e)-259.6(e)0(x)15.1(p)0(r)19.6(e)0(s)19.6(s)0(e)14(d)-268.2(as)-259.2(th)18.9(e)-268.6(m)0(e)17.7(a)0(n)-258(n)0(u)15.5(m)0(b)18.1(e)0(r)]TJ 0 -1.0471 TD [(pe)15.1(r)-508.8(s)0(q)20(u)0(a)15.1(r)0(e)-498.6(m)13.2(il)16.5(li)16.5(me)17.7(tr)17.3(e)-507.6(s)0(u)14.4(b)0(m)18.1(u)0(c)15.1(o)0(s)20(a)-507.6(in)-499(t)13.9(w)0(o)-500.5(s)0(e)19.6(c)0(t)12.8(i)0(o)18.8(n)0(s)20(.)-506.8(Th)17.1(e)]TJ 0 -1.0528 TD [(ex)15.1(pr)19.6(es)13.9(si)17.7(on)-325.9(of)-333.2(en)20.8(do)15.5(th)18.9(el)18.5(ia)18.5(l)-338.9(a)0(d)15.1(h)0(e)20.8(s)0(i)17.7(o)0(n)-331.6(m)0(o)18.1(l)0(e)18.4(c)0(u)15.1(l)0(e)18.4(s)-337.7(wa)16.9(s)-343.4(s)0(h)20(o)0(w)17.3(n)]TJ 0 -1.0471 TD [(as)-361.6(th)13.2(e)-365.4(t)0(o)13.2(t)13.9(al)-362.8(nu)15.5(mb)18.1(er)-362(of)-361.6(ve)20.8(ss)13.2(el)18.4(s)-366.2(s)0(t)17.7(a)0(i)12.8(n)0(i)18.9(n)0(g)-360(p)0(o)15.5(s)0(i)17.7(t)0(i)22.2(v)0(e)15.1(l)0(y)-362.4(f)0(o)19.6(r)-372.2(th)18.9(e)]TJ 0 -1.0527 TD [(ad)15.1(he)15.1(si)17.7(on)-320.2(mo)12.4(l)13.9(e)0(c)14.7(u)0(l)18.9(e)0(s)-327.5(a)0(n)20.8(d)-336.5(pe)20.8(rc)13.5(en)20.8(ta)18.5(ge)-326.3(of)-327.5(to)18.9(ta)18.4(l)-333.2(\()0(E)15.5(N)0(-)15.7(4)0(+)20.6(\))-338.1(ve)20.8(s-)]TJ 0 -1.0471 TD [(se)13.9(ls)17.7(.)]TJ /F4 1 Tf 0 -3.6021 TD [(Dat)-8.6(a)-330.8(analys)-8.6(es)]TJ /F3 1 Tf 1.0015 -2.0998 TD [(FE)12.2(V)]TJ 7.97 0 0 7.97 337.72 305.291 Tm (1)Tj 9.963 0 0 9.963 344.92 305.291 Tm [(wa)16.9(s)-320.6(e)0(x)15.1(p)0(r)19.6(e)0(s)13.9(s)0(e)19.6(d)-319.4(as)-316.1(me)17.7(an)15.1(\213)]TJ 6.974 0 0 6.974 442.318 305.291 Tm 0.0001 Tc (SD)Tj 9.963 0 0 9.963 454.394 305.291 Tm -0.0172 Tc [(p)-17.2(e)3.6(r)-338.2(c)-17.2(en)-17.2(t)-328.4(o)-17.2(f)-333.3(p)-17.2(re)-17.2(di)-17.2(ct)-17.2(ed)]TJ -14.5621 -1.0471 TD 0 Tc [(an)15.1(d)-547.1(P)11.4(EF)-539.8(va)20.8(ri)17.3(ab)15.1(il)16.5(it)22.2(y)-547.1(a)0(s)-538(m)0(e)17.7(a)0(n)15.1(\213)]TJ 6.974 0 0 6.974 435.912 294.86 Tm (SD)Tj 9.963 0 0 9.963 444.642 294.86 Tm [(.)-546.7(C)0(o)16.5(m)0(p)18.1(a)0(r)13.5(i)0(s)17.7(o)0(n)-536.4(o)0(f)-538(l)13.9(un)15.5(g)]TJ -13.5833 -1.0527 TD [(fu)13.9(nc)20.8(ti)16.5(on)-314.5(re)19.2(su)14.4(lt)16.5(s)-320.6(b)0(e)15.1(t)0(w)20.6(e)0(e)14.7(n)-325.1(gr)19.6(ou)21.2(ps)-315.7(wa)16.9(s)-326.3(p)0(e)20.8(r)0(f)18(o)0(r)13.9(m)0(e)17.7(d)-325.1(us)20(in)18.9(g)-325.1(t)0(h)18.9(e)]TJ 0 -1.0471 TD [(un)15.5(pa)15.1(i)13.9(r)0(e)13.5(d)-404.8(t-)17.3(te)18.4(st)17.7(s.)-400.7(A)12.4(s)-406(th)13.2(e)-405.2(i)0(m)15.7(m)0(u)18.1(n)0(o)15.5(h)0(i)18.9(s)0(t)17.7(o)0(c)20.8(h)0(e)15.1(m)0(i)15.7(c)0(a)20.4(l)-407.2(da)15.1(ta)-397(we)16.9(re)]TJ 0 -1.0527 TD [(no)15.5(t)-452.7(n)0(o)21.2(r)0(m)16.5(a)0(l)12.8(l)0(y)-442.1(d)0(i)18.9(s)0(t)17.7(r)0(i)17.3(b)0(u)21.2(t)0(e)12.8(d)0(,)-439.3(t)0(h)18.9(e)-450.7(Kr)15.7(us)14.3(ka)20.8(l-)17.3(W)77.7(a)0(l)18.5(l)0(i)16.5(s)-451.5(\(K)15.7(-W)18.5(\))-451.9(t)0(e)18.4(s)0(t)]TJ 0 -1.0471 TD [(wa)16.9(s)-252.3(u)0(s)14.4(e)0(d)20.8(,)-250.8(an)15.1(d,)-240.2(if)-244.5(si)17.7(gn)21.2(if)17.3(ic)12.8(an)20.8(t,)-242.5(fu)19.6(rt)17.3(he)15.1(r)-252.7(t)0(e)18.4(s)0(t)17.7(e)0(d)-240.9(f)0(o)13.9(r)-247.1(si)17.7(gn)15.5(if)17.3(ic)18.5(an)15.1(ce)]TJ 0 -1.0527 TD [(us)14.4(in)18.8(g)-308.1(t)0(h)18.8(e)-308.5(Ma)13.2(nn)21.2(-W)12.8(hi)18.9(tn)18.8(ey)-303.5(U)12.4(-)0(t)17.3(e)0(s)13.9(t)0(.)-299.4(A)-306.3(Bo)16.5(nf)13.9(er)19.2(on)15.5(ni)-299.8(co)20.8(rr)18(ec)14.7(ti)16.5(on)]TJ 0 -1.0471 TD 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[(lower)-410.5(in)-407.9(the)-409.1(severe)-412.6(asthmati)-12.7(cs)-407.2(\(64.7\21320.6)-7.3(%\))-409.5(compa)-7.8(red)]TJ 0 -1.0471 TD [(with)-347.5(mild)-352.4(asthmati)-12.7(cs)-344.6(\(98.3\21310.7)-7.3(%,)-350.6(p)]TJ /F7 1 Tf 15.08 0 TD 0 Tw (=)Tj /F3 1 Tf 0.552 0 TD [(0.0001\).)-348.4(Both)-351.6(the)]TJ -15.6319 -1.0527 TD [(severe)-236.2(and)-236(mild)-238.6(asthmatics)-243.4(had)-230.3(signi)-9.8(ficantly)-237.6(higher)-236.6(level)-9.4(s)]TJ 0 -1.0471 TD [(of)-247.8(IgE)-247(than)-250.5(the)-249.8(healthy)-249.9(contr)-8.2(ol)-248.6(subjects)-253.1(\(\(median)-254.6(\(range)-8.6(\)\))]TJ 0 -1.0527 TD [(32.5)-338.4(IU)]TJ /F8 1 Tf 3.1298 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (L)Tj 7.472 0 0 6.641 98.192 696.869 Tm (-1)Tj 9.963 0 0 9.963 104.372 692.447 Tm [(,)-330.4(\(10\26175\))-344.3(K-W)25.2(:)-333.2(p)]TJ /F7 1 Tf 7.1246 0 TD (=)Tj /F3 1 Tf 0.552 0 TD (0.001\).)Tj /F4 1 Tf -12.303 -2.8623 TD [(Immuno)-8.3(histochem)-11.9(istry)]TJ /F3 1 Tf 1.0015 -1.6503 TD [(Th)17.1(e)-274.3(u)0(s)14.4(e)-274.3(of)-270.6(th)18.9(e)-274.3(E)0(N)13.2(-)0(4)-264.9(a)0(n)15.1(t)0(i)16.5(b)0(o)21.2(d)0(y)-269(d)0(e)20.8(t)0(e)12.8(c)0(t)18.4(e)0(d)-269.4(m)0(u)18.1(c)0(o)20.8(s)0(a)13.9(l)-276.3(ve)20.8(ss)13.2(el)18.4(s)]TJ -1.0015 -1.0471 TD [(in)-328.3(al)18.4(l)-338.9(t)0(h)18.8(e)-336.9(bi)18.9(op)15.5(si)17.7(es)19.6(,)-341.8(w)12.4(it)16.5(h)-336.5(t)0(h)18.9(e)-342.6(nu)21.2(mb)18.1(er)-333.6(of)-327.5(ve)20.8(ss)18.9(el)12.8(s)-337.7(b)0(e)20.8(i)0(n)18.9(g)-336.5(si)17.7(g-)]TJ 0 -1.0528 TD [(ni)18.9(fi)17.3(ca)14.7(nt)18.9(ly)-277.1(hi)18.9(gh)15.5(er)-276.7(in)-277.1(th)18.9(e)-285.7(s)0(e)19.6(v)0(e)15.1(r)0(e)-276.7(a)0(s)19.6(t)0(h)18.9(m)0(a)12(t)0(i)16.5(c)0(s)-276.3(\()0(9)19.6(1)0(.)15.9(4)]TJ /F8 1 Tf 18.4317 0.1992 TD (.)Tj /F3 1 Tf 0.2675 -0.1992 TD (mm)Tj 7.472 0 0 6.641 259.767 630.992 Tm (-2)Tj 9.963 0 0 9.963 268.668 626.57 Tm [(su)14.4(b-)]TJ -21.1176 -1.0471 TD [(mu)18.1(co)15.1(sa)19.6(\))-565.7(w)0(h)17.3(e)0(n)-559.6(c)0(o)15.1(m)0(p)18.1(a)0(r)19.2(e)0(d)-559.6(t)0(o)-555.9(b)0(o)15.5(t)0(h)-555.9(t)0(h)18.9(e)-570.2(mi)15.7(l)13.9(d)-569.8(as)19.6(th)18.9(ma)12(ti)16.5(cs)]TJ 0 -1.0528 TD [(\(4)19.6(7.)15.9(7)]TJ /F8 1 Tf 2.0258 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (mm)Tj 7.472 0 0 6.641 96.378 610.072 Tm (-2)Tj 9.963 0 0 9.963 106.413 605.65 Tm [(su)20(bm)18.1(uc)15.1(os)20(a\))-390.5(an)15.1(d)-399.1(c)0(o)20.8(n)0(t)18.9(r)0(o)13.9(l)-395.8(su)14.4(bj)18.9(ec)20.4(ts)-392.1(\(4)19.6(8.)15.9(4)]TJ /F8 1 Tf 15.5637 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (mm)Tj 7.472 0 0 6.641 279.383 610.072 Tm (-2)Tj 9.963 0 0 9.963 58.28 595.219 Tm [(su)20(bm)12.4(uc)20.8(os)14.4(a\))19.2(,)-364.6(K)0(-)15.7(W)37.9(:)-361.7(p)]TJ /F7 1 Tf 8.3082 0 TD (=)Tj /F3 1 Tf 0.5463 0 TD [(0.)21.6(00)15.5(9,)-354(\(f)18(ig)13.2(.)-358.9(1)-365(and)-378.3(2\).)-379.1(V)103.4(essel)-379.3(num-)]TJ -8.8545 -1.0528 TD [(bers)-376.1(of)-367.3(two)-372.8(different)-370(subm)-7.4(ucosal)-373.1(areas)-377.7(withi)-9.2(n)-365(the)-375(same)]TJ 0 -1.0471 TD [(biops)-7.4(y)-234.1(speci)-8.2(men)-239.2(show)-8.2(ed)-235.2(a)-240.2(good)-242.1(repeatabil)-12.3(ity)-240.4(with)-239.4(highl)-8.6(y)]TJ 0 -1.0528 TD [(signifi)-9.6(cant)-342(correla)-12.1(tions)-339.8(being)-342.3(found)-346.8(in)-339.7(the)-340.8(overal)-9.4(l)-338.9(group)]TJ 0 -1.0471 TD (\(r)Tj 7.472 0 0 6.641 64.743 551.679 Tm (s)Tj /F7 1 Tf 9.963 0 0 9.963 67.635 553.379 Tm (=)Tj /F3 1 Tf 0.552 0 TD [(0.97,)-446.9(p)]TJ /F7 1 Tf 2.9477 0 TD (=)Tj /F3 1 Tf 0.552 0 TD [(0.0001\))-450.4(and)-446.6(in)-447.8(the)-448.9(severe)-452.4(asthmati)-12.7(c)-445(group)]TJ -4.9906 -1.0528 TD (\(r)Tj 7.472 0 0 6.641 64.743 541.247 Tm (s)Tj /F7 1 Tf 9.963 0 0 9.963 67.635 542.891 Tm (=)Tj /F3 1 Tf 0.552 0 TD [(0.95,)-333.1(p)]TJ /F7 1 Tf 2.8339 0 TD (=)Tj /F3 1 Tf 0.5577 0 TD (0.0004\).)Tj -3.881 -1.0471 TD [(Ex)17.1(pr)19.6(es)14(si)17.7(on)-371.4(of)-367.3(IC)14.9(AM)15.3(-1)-373(wa)16.9(s)-377.5(o)0(b)15.5(s)0(e)19.6(r)0(v)13.9(e)0(d)-366.1(o)0(n)-371.4(t)0(h)13.2(e)-376.7(en)15.1(do)21.2(th)13.2(e-)]TJ -1.0015 -1.0527 TD [(li)16.5(um)-255.1(of)-253.5(th)18.9(e)-262.9(s)0(u)14.4(b)0(m)18.1(u)0(c)15.1(o)0(s)20(a)0(l)-254.7(v)0(e)20.8(n)0(u)15.5(l)0(e)18.4(s)-263.7(an)15.1(d,)-251.5(if)-255.9(pr)19.6(es)13.9(en)20.8(t,)-253.9(al)18.4(so)-253.1(in)-254.3(th)13.2(e)]TJ 0 -1.0471 TD [(ep)15.1(it)22.2(he)15.1(li)16.5(um)18.1(.)-410.1(I)0(n)-401.5(c)0(o)20.8(n)0(t)13.2(r)0(a)19.2(s)0(t)-403.4(V)12.4(CA)12.6(M-)17.7(1,)-399.5(E-)-399.9(an)15.1(d)-410.5(P)11.4(-s)18.4(el)12.8(ec)20.4(ti)16.5(n)-410.5(w)0(a)16.9(s)]TJ 0 -1.0527 TD [(on)15.5(ly)-356.7(se)13.9(en)-360.4(on)-360(bl)18.9(oo)15.5(d)-370.7(v)0(e)20.8(s)0(s)18.9(e)0(l)12.8(s)0(.)-355.2(T)0(h)17.1(e)-371.1(to)18.9(ta)12.8(l)-367.3(n)0(u)15.5(m)0(b)18.1(e)0(r)-362(o)0(f)-361.6(v)0(e)20.8(s)0(s)13.2(e)0(l)18.4(s)]TJ 0 -1.0471 TD [(ex)15.1(pr)19.6(es)19.6(si)17.7(ng)-280.4(IC)14.9(A)12.4(M)0(-)12(1)-285.3(wa)16.9(s)-286.5(s)0(i)17.7(g)0(n)15.5(i)0(f)17.3(i)0(c)18.4(a)0(n)15.1(t)0(l)22.2(y)-291(hi)18.9(gh)21.2(er)-282.4(in)-277.1(th)18.9(e)-291.4(s)0(e)19.6(v)0(e)15.1(r)0(e)]TJ 25.1977 2.8567 TD [(as)13.9(th)18.9(ma)17.7(ti)16.5(cs)-327.5(\(3)19.6(4.)15.9(4)]TJ /F8 1 Tf 6.4474 0.2049 TD (.)Tj /F3 1 Tf 0.2675 -0.2049 TD (mm)Tj 7.472 0 0 6.641 391.408 523.502 Tm (-2)Tj 9.963 0 0 9.963 400.819 519.08 Tm [(su)20(bm)18.1(uc)15.1(os)20(a\))-327.9(th)18.8(an)-320.6(in)-328.3(th)18.8(e)-331.2(m)0(i)15.7(l)0(d)-328.3(a)0(s)19.6(t)0(h)18.8(-)]TJ -9.1845 -1.0698 TD [(ma)12(ti)16.5(cs)-253.5(\(1)13.9(9.)21.6(2)]TJ /F8 1 Tf 4.8199 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (mm)Tj 7.472 0 0 6.641 375.25 512.844 Tm (-2)Tj 9.963 0 0 9.963 383.981 508.422 Tm [(su)20(bm)18.1(uc)15.1(os)20(a\))-259.6(an)20.8(d)-268.2(c)0(o)15.1(n)0(t)18.9(r)0(o)19.6(l)-270.6(su)20(bj)18.9(ec)14.7(ts)-255.5(\(1)13.9(5.)21.6(1)]TJ /F8 1 Tf 15.0344 0.1992 TD (.)Tj /F3 1 Tf -22.5289 -1.2747 TD (mm)Tj 7.472 0 0 6.641 324.51 502.129 Tm (-2)Tj 9.963 0 0 9.963 333.014 497.707 Tm [(su)14.4(bm)18.1(uc)20.8(os)14.4(a,)-234.9(K-)15.7(W)37.9(:)-247.8(p)]TJ /F7 1 Tf 7.7619 0 TD (=)Tj /F3 1 Tf 0.5406 0 TD [(0.)15.9(00)21.2(5;)-237.2(fi)17.3(g.)-240.2(3a)20.8(\).)-258.8(The)-257.2(percent)-10.5(age)]TJ -10.6812 -1.0755 TD [(of)-532.3(ICAM-1)-536.2(expres)-8.2(sion)-530.2(was)-531.3(not)-528.2(signifi)-9.6(cantly)-533.7(different)]TJ T* [(betw)-9.8(een)-230.7(the)-227(three)-236.2(groups:)-232.4(38.4%)-233.4(in)-225.8(the)-232.7(severe)-230.5(asthm)-10.9(atics,)]TJ T* [(36.1)-8.4(%)-355.9(i)0(n)-356.7(the)-357.9(mild)-358.1(asthmati)-12.7(cs,)-356.3(and)-355.5(30.2)-8.4(%)-355.9(i)0(n)-356.7(the)-357.9(control)]TJ T* [(subje)-7.8(cts)-455.8(\(K-)-9.4(W)32.2(:)-458.4(p)]TJ /F7 1 Tf 7.153 0 TD (=)Tj /F3 1 Tf 0.552 0 TD [(0.20;)-461.1(fig.)-456.2(3b\).)-459.5(No)-455(signifi)-9.6(cant)-461.5(dif)10.8(fer-)]TJ -7.705 -1.0698 TD [(enc)-8.8(es)-242.1(were)-248.4(found)-250.1(in)-242.9(the)-249.8(percentage)-252.6(or)-247.8(the)-244.1(total)-250.4(numbe)-7.4(r)-241.4(o)0(f)]TJ 0 -1.0755 TD [(vessel)-10.1(s)-553.9(expressing)-561.1(VCAM-1)-9.3(:)-549.4(medi)-9(an)-553.9(\(range)-8.6(\))-548.7(2.1)-553.9(\(0\261)]TJ T* [(18.8)-8.4(\)%)-346.9(and)-355.5(1.2)]TJ /F8 1 Tf 6.2937 0.1992 TD (.)Tj /F3 1 Tf 0.2675 -0.1992 TD (mm)Tj 7.472 0 0 6.641 390.104 427.181 Tm (-2)Tj 9.963 0 0 9.963 399.742 422.759 Tm [(submucosa)-354.6(\(0\26120.8\))-356(in)-351(the)-352.2(severe)]TJ -9.0764 -1.0755 TD [(asthm)-10.9(atics,)-341(2.7)-337.7(\(0\26113.4\))-8.9(%)-338.9(and)-338.4(1)-6.5(.)0(2)]TJ /F8 1 Tf 13.9475 0.1992 TD (.)Tj /F3 1 Tf 0.2675 -0.1992 TD (mm)Tj 7.472 0 0 6.641 466.356 416.466 Tm (-2)Tj 9.963 0 0 9.963 475.823 412.044 Tm [(subm)-7.4(ucosa)-341.6(\(0\261)]TJ -16.7131 -1.0755 TD [(18.6)-8.4(\))-343.8(i)0(n)-351(the)-346.5(mild)-352.4(asthm)-10.9(atics,)-352.4(and)-349.8(1.2)-349(\(0\2616.1\))-8.2(%)-344.6(and)-349.8(0.7)]TJ /F8 1 Tf 22.5289 0.1992 TD (.)Tj /F3 1 Tf -22.5289 -1.2747 TD (mm)Tj 7.472 0 0 6.641 324.737 395.036 Tm (-2)Tj 9.963 0 0 9.963 334.828 390.614 Tm [(subm)-7.4(ucosa)-404.2(\(0\2613.1\))-400.8(in)-402.2(the)-397.7(contr)-8.2(ol)-402.2(subjects)-406.7(\(K-W)28.5(:)]TJ -2.5607 -1.0698 TD (p)Tj /F7 1 Tf 0.5008 0 TD (=)Tj /F3 1 Tf 0.5577 0 TD [(0.23)-327(and)-332.7(0.30,)-327.4(respec)-8.6(tively\).)-333.5(Simi)-10.2(larly)57.7(,)-324.7(n)0(o)-331.6(dif)10.8(ference)-10.1(s)]TJ -1.0584 -1.0755 TD [(wer)-8.2(e)-314.2(seen)-323.7(in)-316.9(the)-318.1(p)-6.5(ercentage)-325.8(or)-316.1(tota)-8.2(l)-316.1(numbe)-7.4(rs)-317.3(of)-321.8(P-selec-)]TJ T* [(tin+)-310(vessels)-308(\(13.3)-306.6(\(4.8\26141.)-6.9(5\)%)-307.9(and)-304.3(1)27.7(1.0)]TJ /F8 1 Tf 16.1441 0.1992 TD (.)Tj /F3 1 Tf 0.2675 -0.1992 TD (mm)Tj 7.472 0 0 6.641 488.239 362.948 Tm (-2)Tj 9.963 0 0 9.963 497.367 358.526 Tm [(subm)-7.4(uco-)]TJ -18.8755 -1.0755 TD [(sa)-264.9(\(2.6\26123.)-6.9(2\)\))-267.3(in)-265.7(the)-266.8(severe)-264.6(asthm)-10.9(atics,)-267(10.4)-264.4(\(0.9\26149.)-6.9(5\)%)]TJ T* [(and)-315.7(7.6)]TJ /F8 1 Tf 3.0046 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (mm)Tj 7.472 0 0 6.641 357.335 341.518 Tm (-2)Tj 9.963 0 0 9.963 366.576 337.096 Tm [(subm)-7.4(ucosa)-313.1(\(0.6\26133.)-6.9(9\))-310.4(in)-311.2(the)-312.4(mild)-318.2(asthma-)]TJ -5.7475 -1.0755 TD [(tics)-9.4(,)-489.8(and)-497.8(18.9)-497.7(\(10.3\26131)-7.3(.0\)%)-496(and)-497.8(8.4)]TJ /F8 1 Tf 15.4498 0.2049 TD (.)Tj /F3 1 Tf 0.2731 -0.2049 TD (mm)Tj 7.472 0 0 6.641 481.323 330.803 Tm (-2)Tj 9.963 0 0 9.963 492.378 326.381 Tm [(subm)-7.4(ucosa)]TJ -18.3748 -1.0698 TD [(\(4.3\26124.)-6.9(5)-6.5(\))-349.5(in)-356.7(the)-352.2(contr)-8.2(ol)-351(subje)-7.8(cts)-353.4(\(K-)-9.4(W)32.2(:)-356(p)]TJ /F7 1 Tf 17.2993 0 TD (=)Tj /F3 1 Tf 0.552 0 TD [(0.19)-355.5(and)-355.5(p)]TJ /F7 1 Tf 4.3988 0 TD (=)Tj /F3 1 Tf -22.2501 -1.0755 TD [(0.09)-8.4(,)-341.8(respec)-8.6(tively\).)-356.3(In)-344.6(contr)-8.2(ast,)-348.1(the)-352.2(total)-347.1(numbe)-7.4(r)-349.5(o)0(f)-344.6(ves-)]TJ T* [(sels)-366.7(e)-6.9(xpressing)-372.1(E-selectin)-370(was)-372(lower)-364.9(in)-368.1(the)-363.6(severe)-372.8(asth-)]TJ T* [(mat)-8.2(ics)-529.8(\(7.9)]TJ /F8 1 Tf 4.6776 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (mm)Tj 7.472 0 0 6.641 374.06 288 Tm (-2)Tj 9.963 0 0 9.963 385.398 283.578 Tm [(subm)-7.4(ucosa\))-531.7(as)-526.7(compa)-7.8(red)-527.8(with)-529.6(mild)]TJ -7.6367 -1.0755 TD [(asthm)-10.9(atics)-255.3(\(14.1)]TJ /F8 1 Tf 6.5043 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (mm)Tj 7.472 0 0 6.641 392.258 277.285 Tm (-2)Tj 9.963 0 0 9.963 400.932 272.863 Tm [(submucosa\))-265.9(but)-255.1(not)-255.1(different)-256.2(from)]TJ -9.1959 -1.0755 TD [(norm)-7.8(al)-305.9(\(12.4)]TJ /F8 1 Tf 5.1898 0.2049 TD (.)Tj /F3 1 Tf 0.2731 -0.2049 TD (mm)Tj 7.472 0 0 6.641 379.105 266.57 Tm (-2)Tj 9.963 0 0 9.963 388.29 262.148 Tm [(subm)-7.4(ucosa,)-307.8(K-W)25.2(:)-304.7(p)]TJ /F7 1 Tf 8.0066 0 TD (=)Tj /F3 1 Tf 0.552 0 TD [(0.045;)-308.2(\(fig)-8.6(.)-302(4a\).)]TJ -16.4855 -1.0698 TD [(A)-243.7(signifi)-9.6(cant)-245.2(lower)-245.4(percent)-10.5(age)-242.1(of)-242.1(E-selec)-10.1(tin+)-241.7(vessel)-10.1(s)-241(was)]TJ 0 -1.0755 TD [(seen)-232.7(in)-225.8(the)-227(severe)-230.5(asthmati)-12.7(cs)-225.1(\(7.4%\))-231.7(when)-229.3(compa)-7.8(red)-226.2(with)]TJ T* [(the)-215.6(mild)-210.1(asthm)-10.9(atics)-215.4(\(26.5%)-8.2(\))-207.2(and)-213.2(control)-217(subjects)-213.2(\(22.9%)-8.2(\),)]TJ T* [(K-W)25.2(:)-338.9(p)]TJ /F7 1 Tf 3.0615 0 TD (=)Tj /F3 1 Tf 0.552 0 TD [(0.0005)-339.9(\(fig.)-333.4(4)-6.5(b\).)]TJ -2.612 -1.0471 TD [(No)-460.7(si)17.7(gn)21.2(if)17.3(ic)18.4(an)15.1(t)-464.1(d)0(i)18.9(f)20.4(fe)13.5(re)19.2(nc)15.1(es)-452.7(we)16.9(re)-453.1(fo)14(un)21.2(d)-467.4(i)0(n)-453.5(t)0(h)18.9(e)-462.1(me)12(di)18.9(an)]TJ -1.0015 -1.0755 TD [(\(r)12.4(an)20.8(ge)15.1(\))-338.1(n)0(u)21.2(m)0(b)12.4(e)0(r)-327.9(o)0(f)-327.5(L)0(F)91.9(A)0(-)15.7(1)0(+)-332.2(c)0(e)20.4(l)0(l)16.5(s)-337.7(pe)15.1(r)-338.1(s)0(q)20(u)0(a)15.1(r)0(e)-327.9(m)0(i)15.7(l)0(l)16.5(i)13.9(me)12(tr)17.3(e)-336.9(o)0(f)]TJ T* [(su)14.4(bm)18.1(uc)15.1(os)20(a)-564.5(b)0(e)15.1(t)0(w)20.6(e)0(e)14.7(n)-564.1(th)18.9(e)-564.5(s)0(e)13.9(v)0(e)20.8(r)0(e)-555.5(a)0(s)13.9(t)0(h)18.8(m)0(a)17.7(t)0(i)16.5(c)0(s)-555.1(\()0(3)19.6(4)0(.)15.9(7)-564.1(\(8)19.6(.2)15.9(\261)]TJ T* [(15)15.5(3\))19.6(\),)-258.8(th)13.2(e)-268.6(m)0(i)15.7(l)13.9(d)-268.2(a)0(s)13.9(t)0(h)18.9(m)0(a)17.7(t)0(i)16.5(c)0(s)-259.2(\()0(6)13.9(5)0(.)21.6(2)-268.2(\(2)13.9(1.)21.6(2\261)15.5(19)21.2(3\))14(\),)-258.8(an)20.8(d)-268.2(t)0(h)18.8(e)-268.6(co)15.1(n-)]TJ T* [(tr)17.3(ol)-299.8(su)20(bj)13.2(ec)20.4(ts)-301(\(3)19.6(0.)15.9(7)-302.4(\()0(1)14(8)0(.)21.6(6)0(\261)15.5(1)39.1(1)44.8(1)0(.)15.9(8)0(\))19.6(\))0(,)-298.7(K)0(-)15.7(W)37.9(:)-304.7(p)]TJ /F7 1 Tf 16.2465 0 TD (=)Tj /F3 1 Tf 0.5463 0 TD [(0.)21.6(1)39.1(1)0(.)-297.1(S)0(i)13.9(m)0(i)15.7(l)13.9(ar)13.5(ly)81.5(,)]TJ -16.7928 -1.0755 TD [(th)13.2(e)-365.4(n)0(u)21.2(m)0(b)12.4(e)0(r)19.2(s)-366.2(of)-355.9(VL)13.2(A-)15.7(4+)-354.9(ce)14.7(ll)16.5(s)-366.2(w)12.4(er)13.5(e)-365.4(n)0(o)15.5(t)-361.7(si)17.7(gn)15.5(if)17.3(ic)18.4(an)15.1(tl)16.5(y)-365(d)0(i)18.9(f)0(-)]TJ T* [(fe)13.5(re)19.2(nt)-294.1(be)20.8(tw)14.9(ee)20.4(n)-302.4(t)0(h)18.9(e)-302.8(se)19.6(ve)15.1(re)-288.1(as)13.9(t)13.9(h)0(m)12.4(a)0(t)18.4(i)0(c)18.5(s)-297.9(\(1)48.1(1.)15.9(3)]TJ /F8 1 Tf 16.7359 0.2049 TD (.)Tj /F3 1 Tf 0.2732 -0.2049 TD (mm)Tj 7.472 0 0 6.641 493.965 149.102 Tm (-2)Tj 9.963 0 0 9.963 502.979 144.624 Tm [(su)20(bm)12.4(uc)20.8(-)]TJ -19.4389 -1.0698 TD [(os)14.4(a)-257.3(\()0(1)19.6(.)0(6)15.9(\261)0(3)21.2(6)0(.)15.9(2)0(\))19.6(\))0(,)-247.4(t)0(h)18.8(e)-257.2(mi)15.7(ld)-248.6(as)19.6(th)18.9(ma)12(t)13.9(i)0(c)12.8(s)-252.3(\(9)13.9(.6)]TJ /F8 1 Tf 15.797 0.1992 TD (.)Tj /F3 1 Tf 0.2731 -0.1992 TD (mm)Tj 7.472 0 0 6.641 484.611 138.387 Tm (-2)Tj 9.963 0 0 9.963 493.172 133.965 Tm [(su)14.4(bm)18.1(uc)15.1(os)20(a)]TJ -18.4545 -1.0755 TD [(\(0)13.9(.9)21.6(\2613)15.5(6.)21.6(6\))13.9(\),)-224.7(an)15.1(d)-234.1(t)0(h)13.2(e)-234.5(co)15.1(nt)18.9(ro)19.6(l)-236.5(s)0(u)14.4(b)0(j)18.9(e)0(c)20.4(t)0(s)-232.7(\()0(8)19.6(.)0(1)]TJ /F8 1 Tf 15.8197 0.1992 TD (.)Tj /F3 1 Tf 0.2675 -0.1992 TD (mm)Tj 7.472 0 0 6.641 484.781 127.672 Tm (-2)Tj 9.963 0 0 9.963 493.172 123.25 Tm [(su)14.4(bm)18.1(uc)15.1(os)20(a)]TJ -18.4545 -1.0755 TD [(\(1)13.9(.9)21.6(\2611)15.5(0.)21.6(2\))13.9(\),)-315.7(K)12.4(-)0(W)46.9(:)-327.5(p)]TJ /F7 1 Tf 7.9497 0 TD (=)Tj /F3 1 Tf 0.5463 0 TD [(0.)21.6(17)15.5(.)]TJ /F14 1 Tf 9.9 0 0 9.9 59.765 465.687 Tm 0 0 0 1 k /GS3 gs (a\))Tj 0 -16.8485 TD (b\))Tj ET /GS4 gs q 227.8348 0 0 149.0189 60.3266 313.7643 cm /Im1 Do Q q 227.8348 0 0 154.3649 60.3266 141.0351 cm /Im2 Do Q BT /F3 1 Tf 7.97 0 0 7.97 58.28 129.487 Tm 0 g /GS2 gs [(Fig.)-327.1(1.)-331.2(\261)-332.2(Bronchial)-493.7(biopsies)-494.4(showing)-489.3(immunostainin)9.9(g)-495.8(for)-499.1(the)-500.1(vessel)]TJ 0 -1.0599 TD [(marker)-341.7(EN-4)-345.5(in)-339.6(a)-345.4(severe)-341.4(asthmatic)-342.3(\(a\))-348.7(and)-341.2(mild)-345.2(asthmat)10.5(ic)-345.7(subject)-344.9(\(b\),)]TJ 0 -1.067 TD [(respectively)-327.8(\(350)]TJ /F9 1 Tf 6.9069 0 TD (3)Tj /F3 1 Tf 0.8322 0 TD [(magnifica)9.6(tion\).)]TJ ET 0 0 0 1 K 0 J 0 j 0.5 w 3.864 M []0 d /GS4 gs 1 i 349.816 764.38 m 349.816 621.381 l 349.816 621.631 m 539.315 621.631 l 349.879 621.631 m 346.566 621.631 l 349.879 637.131 m 346.566 637.131 l 349.879 652.915 m 346.566 652.915 l 349.879 668.698 m 346.566 668.698 l 349.879 684.482 m 346.566 684.482 l 349.879 700.548 m 346.566 700.548 l 349.879 716.332 m 346.566 716.332 l 349.879 732.115 m 346.566 732.115 l 349.879 748.182 m 346.566 748.182 l 349.879 764.128 m 346.566 764.128 l S BT /F14 1 Tf 10 0 0 10 330.6 760.693 Tm 0 0 0 1 k /GS3 gs -0.0149 Tc (180)Tj -0.0216 -1.6255 TD 0 Tc (160)Tj -0.0096 -1.5616 TD (140)Tj 0 -1.5812 TD (120)Tj 0 -1.575 TD (100)Tj 0.4625 -1.5937 TD -0.0053 Tc (80)Tj 0 -1.6009 TD (60)Tj 0 -1.5708 TD (40)Tj 0.0125 -1.6134 TD -0.0178 Tc (20)Tj 0.4438 -1.5317 TD 0 Tc (0)Tj ET /GS4 gs 381.629 621.631 m 381.629 618.881 l 444.628 621.631 m 444.628 618.881 l 507.344 621.631 m 507.344 618.881 l S BT 10 0 0 10 494.457 608.761 Tm /GS3 gs (Control)Tj -0.1824 -1 TD (subjects)Tj -5.5647 1.023 TD (Mild)Tj -1.2074 -1 TD (asthmatics)Tj -5.6127 0.977 TD [(Se)30(v)25(ere)]TJ -0.6875 -1 TD (asthmatics)Tj 0 10 -10 0 319.316 632.647 Tm [(EN-4+ v)25(essels\341mm)]TJ 0 8 -8 0 317.049 701.426 Tm (-2)Tj 0 10 -10 0 319.316 707.258 Tm [( subm)10(ucosa)]TJ ET /GS4 gs 386.632 655.688 m 386.632 656.862 387.583 657.813 388.757 657.813 c 389.931 657.813 390.882 656.862 390.882 655.688 c 390.882 654.515 389.931 653.564 388.757 653.564 c 387.583 653.564 386.632 654.515 386.632 655.688 c b 379.441 653.939 m 379.441 655.112 380.393 656.063 381.566 656.063 c 382.74 656.063 383.691 655.112 383.691 653.939 c 383.691 652.765 382.74 651.813 381.566 651.813 c 380.393 651.813 379.441 652.765 379.441 653.939 c b 379.441 658.313 m 379.441 659.487 380.393 660.438 381.566 660.438 c 382.74 660.438 383.691 659.487 383.691 658.313 c 383.691 657.14 382.74 656.189 381.566 656.189 c 380.393 656.189 379.441 657.14 379.441 658.313 c b 379.441 665.188 m 379.441 666.362 380.393 667.313 381.566 667.313 c 382.74 667.313 383.691 666.362 383.691 665.188 c 383.691 664.015 382.74 663.063 381.566 663.063 c 380.393 663.063 379.441 664.015 379.441 665.188 c b 379.441 671.313 m 379.441 672.487 380.393 673.438 381.566 673.438 c 382.74 673.438 383.691 672.487 383.691 671.313 c 383.691 670.14 382.74 669.188 381.566 669.188 c 380.393 669.188 379.441 670.14 379.441 671.313 c b 379.441 680.063 m 379.441 681.237 380.393 682.188 381.566 682.188 c 382.74 682.188 383.691 681.237 383.691 680.063 c 383.691 678.89 382.74 677.938 381.566 677.938 c 380.393 677.938 379.441 678.89 379.441 680.063 c b 379.441 686.188 m 379.441 687.362 380.393 688.313 381.566 688.313 c 382.74 688.313 383.691 687.362 383.691 686.188 c 383.691 685.015 382.74 684.063 381.566 684.063 c 380.393 684.063 379.441 685.015 379.441 686.188 c b 379.441 692.938 m 379.441 694.112 380.393 695.063 381.566 695.063 c 382.74 695.063 383.691 694.112 383.691 692.938 c 383.691 691.765 382.74 690.813 381.566 690.813 c 380.393 690.813 379.441 691.765 379.441 692.938 c b 379.441 696.938 m 379.441 698.112 380.393 699.063 381.566 699.063 c 382.74 699.063 383.691 698.112 383.691 696.938 c 383.691 695.765 382.74 694.813 381.566 694.813 c 380.393 694.813 379.441 695.765 379.441 696.938 c b 379.441 701.779 m 379.441 702.953 380.393 703.905 381.566 703.905 c 382.74 703.905 383.691 702.953 383.691 701.779 c 383.691 700.606 382.74 699.655 381.566 699.655 c 380.393 699.655 379.441 700.606 379.441 701.779 c b 379.441 706.188 m 379.441 707.362 380.393 708.313 381.566 708.313 c 382.74 708.313 383.691 707.362 383.691 706.188 c 383.691 705.015 382.74 704.063 381.566 704.063 c 380.393 704.063 379.441 705.015 379.441 706.188 c b 379.441 710.813 m 379.441 711.987 380.393 712.938 381.566 712.938 c 382.74 712.938 383.691 711.987 383.691 710.813 c 383.691 709.64 382.74 708.688 381.566 708.688 c 380.393 708.688 379.441 709.64 379.441 710.813 c b 379.441 729.938 m 379.441 731.112 380.393 732.063 381.566 732.063 c 382.74 732.063 383.691 731.112 383.691 729.938 c 383.691 728.764 382.74 727.813 381.566 727.813 c 380.393 727.813 379.441 728.764 379.441 729.938 c b 386.566 708.938 m 386.566 710.112 387.517 711.063 388.691 711.063 c 389.865 711.063 390.816 710.112 390.816 708.938 c 390.816 707.765 389.865 706.813 388.691 706.813 c 387.517 706.813 386.566 707.765 386.566 708.938 c b 379.441 756.688 m 379.441 757.862 380.393 758.813 381.566 758.813 c 382.74 758.813 383.691 757.862 383.691 756.688 c 383.691 755.514 382.74 754.563 381.566 754.563 c 380.393 754.563 379.441 755.514 379.441 756.688 c b 442.882 636.063 m 442.882 637.237 443.833 638.189 445.007 638.189 c 446.181 638.189 447.132 637.237 447.132 636.063 c 447.132 634.89 446.181 633.939 445.007 633.939 c 443.833 633.939 442.882 634.89 442.882 636.063 c b 442.632 648.314 m 442.632 649.487 443.583 650.438 444.757 650.438 c 445.93 650.438 446.882 649.487 446.882 648.314 c 446.882 647.14 445.93 646.188 444.757 646.188 c 443.583 646.188 442.632 647.14 442.632 648.314 c b 449.632 650.564 m 449.632 651.737 450.583 652.688 451.757 652.688 c 452.93 652.688 453.882 651.737 453.882 650.564 c 453.882 649.39 452.93 648.438 451.757 648.438 c 450.583 648.438 449.632 649.39 449.632 650.564 c b 449.632 655.814 m 449.632 656.987 450.583 657.938 451.757 657.938 c 452.93 657.938 453.882 656.987 453.882 655.814 c 453.882 654.64 452.93 653.688 451.757 653.688 c 450.583 653.688 449.632 654.64 449.632 655.814 c b 443.132 653.588 m 443.132 654.761 444.083 655.713 445.257 655.713 c 446.43 655.713 447.382 654.761 447.382 653.588 c 447.382 652.414 446.43 651.463 445.257 651.463 c 444.083 651.463 443.132 652.414 443.132 653.588 c b 436.382 652.688 m 436.382 653.862 437.333 654.813 438.507 654.813 c 439.68 654.813 440.632 653.862 440.632 652.688 c 440.632 651.515 439.68 650.564 438.507 650.564 c 437.333 650.564 436.382 651.515 436.382 652.688 c b 436.007 658.063 m 436.007 659.237 436.958 660.188 438.132 660.188 c 439.305 660.188 440.257 659.237 440.257 658.063 c 440.257 656.89 439.305 655.938 438.132 655.938 c 436.958 655.938 436.007 656.89 436.007 658.063 c b 442.882 659.063 m 442.882 660.237 443.833 661.188 445.007 661.188 c 446.181 661.188 447.132 660.237 447.132 659.063 c 447.132 657.89 446.181 656.939 445.007 656.939 c 443.833 656.939 442.882 657.89 442.882 659.063 c b 442.882 664.289 m 442.882 665.462 443.833 666.414 445.007 666.414 c 446.181 666.414 447.132 665.462 447.132 664.289 c 447.132 663.115 446.181 662.164 445.007 662.164 c 443.833 662.164 442.882 663.115 442.882 664.289 c b 435.632 664.289 m 435.632 665.462 436.583 666.414 437.757 666.414 c 438.93 666.414 439.882 665.462 439.882 664.289 c 439.882 663.115 438.93 662.164 437.757 662.164 c 436.583 662.164 435.632 663.115 435.632 664.289 c b 442.757 670.289 m 442.757 671.462 443.708 672.414 444.882 672.414 c 446.055 672.414 447.007 671.462 447.007 670.289 c 447.007 669.115 446.055 668.164 444.882 668.164 c 443.708 668.164 442.757 669.115 442.757 670.289 c b 442.757 675.914 m 442.757 677.087 443.708 678.039 444.882 678.039 c 446.055 678.039 447.007 677.087 447.007 675.914 c 447.007 674.74 446.055 673.789 444.882 673.789 c 443.708 673.789 442.757 674.74 442.757 675.914 c b 443.007 689.539 m 443.007 690.712 443.958 691.664 445.132 691.664 c 446.305 691.664 447.257 690.712 447.257 689.539 c 447.257 688.365 446.305 687.414 445.132 687.414 c 443.958 687.414 443.007 688.365 443.007 689.539 c b 449.632 670.664 m 449.632 671.837 450.583 672.789 451.757 672.789 c 452.93 672.789 453.882 671.837 453.882 670.664 c 453.882 669.49 452.93 668.539 451.757 668.539 c 450.583 668.539 449.632 669.49 449.632 670.664 c b 442.882 731.288 m 442.882 732.462 443.833 733.413 445.007 733.413 c 446.181 733.413 447.132 732.462 447.132 731.288 c 447.132 730.115 446.181 729.163 445.007 729.163 c 443.833 729.163 442.882 730.115 442.882 731.288 c b 505.506 645.813 m 505.506 646.987 506.458 647.939 507.632 647.939 c 508.805 647.939 509.756 646.987 509.756 645.813 c 509.756 644.64 508.805 643.688 507.632 643.688 c 506.458 643.688 505.506 644.64 505.506 645.813 c b 512.381 650.063 m 512.381 651.237 513.333 652.188 514.506 652.188 c 515.68 652.188 516.631 651.237 516.631 650.063 c 516.631 648.89 515.68 647.939 514.506 647.939 c 513.333 647.939 512.381 648.89 512.381 650.063 c b 505.631 652.371 m 505.631 653.545 506.583 654.496 507.756 654.496 c 508.93 654.496 509.882 653.545 509.882 652.371 c 509.882 651.198 508.93 650.246 507.756 650.246 c 506.583 650.246 505.631 651.198 505.631 652.371 c b 505.382 658.121 m 505.382 659.295 506.333 660.246 507.506 660.246 c 508.68 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509.882 667.313 c 509.882 666.14 508.93 665.188 507.756 665.188 c 506.583 665.188 505.631 666.14 505.631 667.313 c b BT 10 0 0 10 398.816 760.49 Tm /GS3 gs (p=0.02)Tj 3.4375 0.9265 TD (p=0.03)Tj 8 0 0 8 470.599 760.49 Tm (NS)Tj ET /GS4 gs 379.441 760.005 m 379.441 763.131 l 398.066 763.131 l 424.941 763.131 m 441.441 763.131 l 441.441 760.005 l 379.441 769.542 m 379.441 772.542 l 432.816 772.542 l 444.191 760.005 m 444.191 763.005 l 469.316 763.005 l 480.941 763.005 m 505.565 763.005 l 505.565 760.005 l 459.532 772.542 m 505.532 772.542 l 505.532 769.917 l S BT /F3 1 Tf 7.97 0 0 7.97 309.316 584.56 Tm 0 g /GS2 gs [(Fig.)-334.3(2.)-324.1(\261)-332.2(Density)-504.9(of)-508.1(the)-507.2(subepith)11.9(elial)-513.9(microvasc)10.8(ulature)-507.5(expressed)-499.9(as)]TJ 0 -1.0599 TD [(numbers)-469.6(of)-465.5(the)-471.7(vessel)-466.7(marker)35.3(,)-468.4(EN-4+,)-465.3(vessels)-465.8(in)-474.8(the)-464.5(submucosa)-465.4(of)]TJ T* 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[(au)15.1(th)18.8(or)13.9(s)-462.9(d)0(i)18.8(d)-461.7(no)15.5(t)-458.4(s)0(t)17.7(u)0(d)15.5(y)-461.7(an)-457.2(ad)20.8(di)18.9(ti)16.5(on)15.5(al)-453.9(gr)19.6(ou)15.5(p)-461.7(o)0(f)-452.7(w)0(e)16.9(l)0(l)16.5(-)0(c)13.5(o)0(n)21.2(-)]TJ T* [(tr)17.3(ol)13.2(le)18.4(d)-387.7(a)0(s)13.9(t)0(h)18.8(m)0(a)17.7(t)0(i)16.5(c)0(s)-384.4(o)0(n)-382.8(h)0(i)18.9(g)0(h)-382.8(d)0(o)21.2(s)0(e)13.9(s)-388.9(of)-384.4(gl)18.9(uc)15.1(oc)20.8(or)13.9(ti)16.5(co)20.8(id)18.9(s.)-383.6(Th)17.1(e)]TJ T* [(pr)13.9(im)15.7(ar)19.2(y)-336.5(g)0(o)21.2(a)0(l)-328.7(o)0(f)-321.8(t)0(h)13.2(i)0(s)-323.8(a)0(n)15.1(d)-330.8(pr)13.9(ev)20.8(io)18.9(us)-327.1(st)17.7(ud)15.5(ie)18.4(s)-332([)0(1)48.1(1)0(])-338.9(was)-343.6(to)-345.3(in-)]TJ T* [(vesti)-10.2(gate)-250.9(whether)-258.7(differences)-251.7(in)-248.6(VCA)-9.2(M)-247.4(expres)-8.2(sion)-251.3(could)]TJ T* [(acco)-10(unt)-397.3(for)-398.1(the)-403.4(dif)10.8(ference)-10.2(s)-400.3(i)0(n)-396.6(inflamm)-12.7(atory)-400.8(cell)-401.3(profile)]TJ T* [(betw)-9.8(een)-264.9(severe)-270.3(and)-264.5(mild)-267(asthmati)-12.7(cs)-259.2([1)25.3(1],)-265.3(and)-264.5(the)-266.8(authors)]TJ 0 -1.0698 TD [(there)-8.6(fore)-433.4(speci)-8.2(fically)-436(selec)-8.6(ted)-431.9(pati)-8.2(ents)-433.8(who)-433(represented)]TJ 0 -1.0755 TD [(the)-614(tw)-7.8(o)-609.7(extre)-8.6(mes)-615.9(of)-612(the)-614(disea)-8.2(se)-612(spectrum.)-618.8(Previo)-8.2(us)]TJ T* [(studi)-9.8(es)-418.5(have)-425(alrea)-9(dy)-422.6(shown)-424.3(that)-423.6(treatmen)-11.1(t)-418.6(o)-6.5(f)-417.8(mild)-426.4(and)]TJ T* [(moder)-9(atel)-8.6(y)-325.1(severe)-332.9(asthmati)-12.7(cs)-327.5(with)-330.4(glucoc)-7.8(orticoids)-335.9(is)-329.5(as-)]TJ T* [(socia)-8.2(ted)-557.1(wi)-7.8(th)-555.9(a)-558.8(marked)-562.1(depletion)-566.8(of)-555.1(eosin)-7.8(ophils)-562.5(and)]TJ ET 0 0 0 1 K 0 J 0 j 0.485 w 3.864 M []0 d /GS4 gs 1 i 99.391 763.066 m 99.391 624.356 l 99.391 624.598 m 283.206 624.598 l 99.452 624.598 m 96.239 624.598 l 99.452 639.633 m 96.239 639.633 l 99.452 654.943 m 96.239 654.943 l 99.452 670.254 m 96.239 670.254 l 99.452 685.563 m 96.239 685.563 l 99.452 701.148 m 96.239 701.148 l 99.452 716.458 m 96.239 716.458 l 99.452 731.768 m 96.239 731.768 l 99.452 747.353 m 96.239 747.353 l 99.452 762.821 m 96.239 762.821 l S BT /F14 1 Tf 9.7 0 0 9.7 85.024 744.021 Tm 0 0 0 1 k /GS3 gs -0.0053 Tc (60)Tj -0.0125 -6.3666 TD (40)Tj 0.0125 -6.2684 TD -0.0178 Tc (20)Tj ET /GS4 gs 130.249 624.598 m 130.249 621.931 l 191.359 624.598 m 191.359 621.931 l 252.194 624.598 m 252.194 621.931 l S 132.678 633.99 m 132.678 635.129 133.601 636.052 134.739 636.052 c 135.877 636.052 136.8 635.129 136.8 633.99 c 136.8 632.852 135.877 631.929 134.739 631.929 c 133.601 631.929 132.678 632.852 132.678 633.99 c b BT 9.7 0 0 9.7 85.101 636.246 Tm /GS3 gs -0.0116 Tc (25)Tj -0.015 1.5871 TD 0 Tc (30)Tj 0.0212 1.5821 TD -0.0178 Tc (35)Tj -0.0062 9.5396 TD -0.0116 Tc (65)Tj 0.0062 -3.2034 TD -0.0178 Tc (55)Tj 0 -1.5633 TD (50)Tj -0.0125 -1.5895 TD -0.0053 Tc (45)Tj 0 9.7 -9.7 0 68.57 628.61 Tm 0 Tc [(ICAM-1+ v)25(essels\341EN-4+ v)25(essels)]TJ 0 7.76 -7.76 0 66.371 742.168 Tm (-1)Tj 0 9.7 -9.7 0 68.57 747.825 Tm ( %)Tj ET /GS4 gs 132.92 649.51 m 132.92 650.649 133.843 651.572 134.982 651.572 c 136.12 651.572 137.043 650.649 137.043 649.51 c 137.043 648.372 136.12 647.449 134.982 647.449 c 133.843 647.449 132.92 648.372 132.92 649.51 c b 126.373 663.818 m 126.373 664.956 127.296 665.879 128.434 665.879 c 129.572 665.879 130.495 664.956 130.495 663.818 c 130.495 662.679 129.572 661.756 128.434 661.756 c 127.296 661.756 126.373 662.679 126.373 663.818 c b 132.678 666.122 m 132.678 667.26 133.601 668.183 134.739 668.183 c 135.877 668.183 136.8 667.26 136.8 666.122 c 136.8 664.983 135.877 664.06 134.739 664.06 c 133.601 664.06 132.678 664.983 132.678 666.122 c b 140.195 664.909 m 140.195 666.047 141.118 666.97 142.257 666.97 c 143.395 666.97 144.318 666.047 144.318 664.909 c 144.318 663.771 143.395 662.848 142.257 662.848 c 141.118 662.848 140.195 663.771 140.195 664.909 c b 126.737 678.368 m 126.737 679.506 127.66 680.429 128.798 680.429 c 129.936 680.429 130.859 679.506 130.859 678.368 c 130.859 677.229 129.936 676.307 128.798 676.307 c 127.66 676.307 126.737 677.229 126.737 678.368 c b 132.92 680.793 m 132.92 681.931 133.843 682.854 134.982 682.854 c 136.12 682.854 137.043 681.931 137.043 680.793 c 137.043 679.654 136.12 678.732 134.982 678.732 c 133.843 678.732 132.92 679.654 132.92 680.793 c b 139.953 680.55 m 139.953 681.689 140.876 682.612 142.014 682.612 c 143.152 682.612 144.075 681.689 144.075 680.55 c 144.075 679.412 143.152 678.489 142.014 678.489 c 140.876 678.489 139.953 679.412 139.953 680.55 c b 123.02 681.039 m 150.301 681.039 l S 140.317 688.431 m 140.317 689.57 141.239 690.493 142.378 690.493 c 143.516 690.493 144.439 689.57 144.439 688.431 c 144.439 687.293 143.516 686.37 142.378 686.37 c 141.239 686.37 140.317 687.293 140.317 688.431 c b 132.678 689.28 m 132.678 690.419 133.601 691.341 134.739 691.341 c 135.877 691.341 136.8 690.419 136.8 689.28 c 136.8 688.142 135.877 687.219 134.739 687.219 c 133.601 687.219 132.678 688.142 132.678 689.28 c b 132.799 699.587 m 132.799 700.725 133.722 701.648 134.86 701.648 c 135.999 701.648 136.922 700.725 136.922 699.587 c 136.922 698.448 135.999 697.525 134.86 697.525 c 133.722 697.525 132.799 698.448 132.799 699.587 c b 140.317 702.012 m 140.317 703.15 141.239 704.073 142.378 704.073 c 143.516 704.073 144.439 703.15 144.439 702.012 c 144.439 700.873 143.516 699.95 142.378 699.95 c 141.239 699.95 140.317 700.873 140.317 702.012 c b 132.92 705.164 m 132.92 706.302 133.843 707.225 134.982 707.225 c 136.12 707.225 137.043 706.302 137.043 705.164 c 137.043 704.026 136.12 703.103 134.982 703.103 c 133.843 703.103 132.92 704.026 132.92 705.164 c b 132.799 722.139 m 132.799 723.277 133.722 724.2 134.86 724.2 c 135.999 724.2 136.922 723.277 136.922 722.139 c 136.922 721.001 135.999 720.078 134.86 720.078 c 133.722 720.078 132.799 721.001 132.799 722.139 c b 132.314 758.756 m 132.314 759.895 133.237 760.818 134.375 760.818 c 135.514 760.818 136.437 759.895 136.437 758.756 c 136.437 757.618 135.514 756.695 134.375 756.695 c 133.237 756.695 132.314 757.618 132.314 758.756 c b 202.924 725.296 m 202.924 726.434 203.846 727.357 204.985 727.357 c 206.123 727.357 207.046 726.434 207.046 725.296 c 207.046 724.157 206.123 723.234 204.985 723.234 c 203.846 723.234 202.924 724.157 202.924 725.296 c b 203.045 718.505 m 203.045 719.644 203.968 720.567 205.106 720.567 c 206.244 720.567 207.167 719.644 207.167 718.505 c 207.167 717.367 206.244 716.444 205.106 716.444 c 203.968 716.444 203.045 717.367 203.045 718.505 c b 202.681 708.32 m 202.681 709.459 203.604 710.382 204.742 710.382 c 205.881 710.382 206.803 709.459 206.803 708.32 c 206.803 707.182 205.881 706.259 204.742 706.259 c 203.604 706.259 202.681 707.182 202.681 708.32 c b 202.802 697.772 m 202.802 698.91 203.725 699.833 204.863 699.833 c 206.002 699.833 206.925 698.91 206.925 697.772 c 206.925 696.633 206.002 695.711 204.863 695.711 c 203.725 695.711 202.802 696.633 202.802 697.772 c b 202.924 693.043 m 202.924 694.181 203.846 695.104 204.985 695.104 c 206.123 695.104 207.046 694.181 207.046 693.043 c 207.046 691.905 206.123 690.982 204.985 690.982 c 203.846 690.982 202.924 691.905 202.924 693.043 c b 202.924 688.839 m 202.924 689.978 203.846 690.901 204.985 690.901 c 206.123 690.901 207.046 689.978 207.046 688.839 c 207.046 687.701 206.123 686.778 204.985 686.778 c 203.846 686.778 202.924 687.701 202.924 688.839 c b 202.924 684.337 m 202.924 685.475 203.846 686.398 204.985 686.398 c 206.123 686.398 207.046 685.475 207.046 684.337 c 207.046 683.199 206.123 682.276 204.985 682.276 c 203.846 682.276 202.924 683.199 202.924 684.337 c b 202.56 673.789 m 202.56 674.927 203.483 675.85 204.621 675.85 c 205.759 675.85 206.682 674.927 206.682 673.789 c 206.682 672.65 205.759 671.727 204.621 671.727 c 203.483 671.727 202.56 672.65 202.56 673.789 c b 202.924 668.938 m 202.924 670.077 203.846 671 204.985 671 c 206.123 671 207.046 670.077 207.046 668.938 c 207.046 667.8 206.123 666.877 204.985 666.877 c 203.846 666.877 202.924 667.8 202.924 668.938 c b 202.907 664.573 m 202.907 665.712 203.83 666.635 204.969 666.635 c 206.107 666.635 207.03 665.712 207.03 664.573 c 207.03 663.435 206.107 662.512 204.969 662.512 c 203.83 662.512 202.907 663.435 202.907 664.573 c b 209.35 661.663 m 209.35 662.802 210.273 663.725 211.411 663.725 c 212.549 663.725 213.472 662.802 213.472 661.663 c 213.472 660.525 212.549 659.602 211.411 659.602 c 210.273 659.602 209.35 660.525 209.35 661.663 c b 202.681 658.753 m 202.681 659.892 203.604 660.815 204.742 660.815 c 205.881 660.815 206.803 659.892 206.803 658.753 c 206.803 657.615 205.881 656.692 204.742 656.692 c 203.604 656.692 202.681 657.615 202.681 658.753 c b 202.802 650.751 m 202.802 651.889 203.725 652.812 204.863 652.812 c 206.002 652.812 206.925 651.889 206.925 650.751 c 206.925 649.613 206.002 648.69 204.863 648.69 c 203.725 648.69 202.802 649.613 202.802 650.751 c b 209.35 633.17 m 209.35 634.308 210.273 635.231 211.411 635.231 c 212.549 635.231 213.472 634.308 213.472 633.17 c 213.472 632.031 212.549 631.108 211.411 631.108 c 210.273 631.108 209.35 632.031 209.35 633.17 c b 202.802 630.502 m 202.802 631.641 203.725 632.563 204.863 632.563 c 206.002 632.563 206.925 631.641 206.925 630.502 c 206.925 629.364 206.002 628.441 204.863 628.441 c 203.725 628.441 202.802 629.364 202.802 630.502 c b 268.277 634.261 m 268.277 635.399 269.2 636.322 270.338 636.322 c 271.477 636.322 272.4 635.399 272.4 634.261 c 272.4 633.123 271.477 632.2 270.338 632.2 c 269.2 632.2 268.277 633.123 268.277 634.261 c b 268.398 639.232 m 268.398 640.371 269.321 641.293 270.46 641.293 c 271.598 641.293 272.521 640.371 272.521 639.232 c 272.521 638.094 271.598 637.171 270.46 637.171 c 269.321 637.171 268.398 638.094 268.398 639.232 c b 268.398 647.841 m 268.398 648.979 269.321 649.902 270.46 649.902 c 271.598 649.902 272.521 648.979 272.521 647.841 c 272.521 646.703 271.598 645.78 270.46 645.78 c 269.321 645.78 268.398 646.703 268.398 647.841 c b 275.916 645.901 m 275.916 647.039 276.839 647.962 277.977 647.962 c 279.116 647.962 280.038 647.039 280.038 645.901 c 280.038 644.763 279.116 643.84 277.977 643.84 c 276.839 643.84 275.916 644.763 275.916 645.901 c b 268.641 664.21 m 268.641 665.348 269.564 666.271 270.702 666.271 c 271.841 666.271 272.763 665.348 272.763 664.21 c 272.763 663.071 271.841 662.149 270.702 662.149 c 269.564 662.149 268.641 663.071 268.641 664.21 c b 268.277 684.822 m 268.277 685.961 269.2 686.883 270.338 686.883 c 271.477 686.883 272.4 685.961 272.4 684.822 c 272.4 683.684 271.477 682.761 270.338 682.761 c 269.2 682.761 268.277 683.684 268.277 684.822 c b 276.037 683.246 m 276.037 684.384 276.96 685.307 278.098 685.307 c 279.237 685.307 280.16 684.384 280.16 683.246 c 280.16 682.108 279.237 681.185 278.098 681.185 c 276.96 681.185 276.037 682.108 276.037 683.246 c b 268.156 693.189 m 268.156 694.327 269.079 695.25 270.217 695.25 c 271.355 695.25 272.278 694.327 272.278 693.189 c 272.278 692.05 271.355 691.127 270.217 691.127 c 269.079 691.127 268.156 692.05 268.156 693.189 c b 191.647 673.764 m 218.686 673.764 l 259.911 655.698 m 284.767 655.698 l 133.69 771.275 m 133.69 773.943 l 197.71 773.943 l 133.811 763.247 m 133.811 765.914 l 161.214 765.914 l 175.279 766.035 m 202.438 766.035 l 202.438 763.247 l 205.348 763.247 m 205.348 766.035 l 232.63 766.035 l 246.573 766.035 m 273.976 766.035 l 273.976 763.247 l 209.471 773.943 m 273.733 773.943 l 273.733 771.275 l S BT 7.76 0 0 7.76 199.286 771.275 Tm /GS3 gs (NS)Tj 4.6583 -1.0263 TD (NS)Tj -9.3219 0.0104 TD (NS)Tj 9.7 0 0 9.7 61.029 766.795 Tm -0.0038 Tc (a\))Tj ET /GS4 gs 99.666 591.788 m 99.666 453.079 l 99.666 453.321 m 283.481 453.321 l 99.727 453.321 m 96.514 453.321 l 99.727 476.19 m 96.514 476.19 l 99.727 499.113 m 96.514 499.113 l 99.727 522.174 m 96.514 522.174 l 99.727 545.181 m 96.514 545.181 l 99.727 568.188 m 96.514 568.188 l 99.727 591.544 m 96.514 591.544 l S BT 9.7 0 0 9.7 85.024 495.977 Tm /GS3 gs -0.0178 Tc (20)Tj ET /GS4 gs 130.524 453.321 m 130.524 450.654 l 191.634 453.321 m 191.634 450.654 l 252.469 453.321 m 252.469 450.654 l S BT 9.7 0 0 9.7 239.969 440.837 Tm /GS3 gs 0 Tc (Control)Tj -0.1824 -1 TD (subjects)Tj -5.5647 1.023 TD (Mild)Tj -1.2074 -1 TD (asthmatics)Tj -5.6127 0.977 TD [(Se)30(v)25(ere)]TJ -0.6875 -1 TD (asthmatics)Tj -5.1644 16.9465 TD -0.0038 Tc (b\))Tj 2.4408 -0.7292 TD 0 Tc (60)Tj 0.0017 -2.4309 TD (50)Tj -0.0175 -2.392 TD -0.0116 Tc (40)Tj 0.0158 -2.3288 TD 0 Tc (30)Tj 0.0017 -4.7484 TD (10)Tj 0.45 -2.3483 TD (0)Tj 0 9.7 -9.7 0 68.651 457.448 Tm [(ICAM-1+ v)25(essels\341min)]TJ 0 7.76 -7.76 0 65.902 532.565 Tm (-2)Tj 0 9.7 -9.7 0 68.651 538.222 Tm [( subm)10(ucosa)]TJ 0 7.76 -7.76 0 66.452 581.009 Tm (-1)Tj ET /GS4 gs 128.345 480.173 m 128.345 481.311 129.268 482.234 130.406 482.234 c 131.545 482.234 132.468 481.311 132.468 480.173 c 132.468 479.035 131.545 478.112 130.406 478.112 c 129.268 478.112 128.345 479.035 128.345 480.173 c b 128.345 486.357 m 128.345 487.495 129.268 488.418 130.406 488.418 c 131.545 488.418 132.468 487.495 132.468 486.357 c 132.468 485.218 131.545 484.295 130.406 484.295 c 129.268 484.295 128.345 485.218 128.345 486.357 c b 128.345 497.269 m 128.345 498.408 129.268 499.33 130.406 499.33 c 131.545 499.33 132.468 498.408 132.468 497.269 c 132.468 496.131 131.545 495.208 130.406 495.208 c 129.268 495.208 128.345 496.131 128.345 497.269 c b 128.345 508.545 m 128.345 509.684 129.268 510.607 130.406 510.607 c 131.545 510.607 132.468 509.684 132.468 508.545 c 132.468 507.407 131.545 506.484 130.406 506.484 c 129.268 506.484 128.345 507.407 128.345 508.545 c b 128.467 513.032 m 128.467 514.17 129.389 515.093 130.528 515.093 c 131.666 515.093 132.589 514.17 132.589 513.032 c 132.589 511.893 131.666 510.97 130.528 510.97 c 129.389 510.97 128.467 511.893 128.467 513.032 c b 135.135 511.092 m 135.135 512.23 136.058 513.153 137.196 513.153 c 138.335 513.153 139.258 512.23 139.258 511.092 c 139.258 509.953 138.335 509.03 137.196 509.03 c 136.058 509.03 135.135 509.953 135.135 511.092 c b 128.588 525.52 m 128.588 526.659 129.511 527.582 130.649 527.582 c 131.787 527.582 132.71 526.659 132.71 525.52 c 132.71 524.382 131.787 523.459 130.649 523.459 c 129.511 523.459 128.588 524.382 128.588 525.52 c b 128.709 532.432 m 128.709 533.57 129.632 534.493 130.77 534.493 c 131.909 534.493 132.831 533.57 132.831 532.432 c 132.831 531.293 131.909 530.37 130.77 530.37 c 129.632 530.37 128.709 531.293 128.709 532.432 c b 128.709 543.223 m 128.709 544.361 129.632 545.284 130.77 545.284 c 131.909 545.284 132.831 544.361 132.831 543.223 c 132.831 542.084 131.909 541.162 130.77 541.162 c 129.632 541.162 128.709 542.084 128.709 543.223 c b 128.709 550.498 m 128.709 551.636 129.632 552.559 130.77 552.559 c 131.909 552.559 132.831 551.636 132.831 550.498 c 132.831 549.359 131.909 548.437 130.77 548.437 c 129.632 548.437 128.709 549.359 128.709 550.498 c b 128.709 562.986 m 128.709 564.125 129.632 565.048 130.77 565.048 c 131.909 565.048 132.831 564.125 132.831 562.986 c 132.831 561.848 131.909 560.925 130.77 560.925 c 129.632 560.925 128.709 561.848 128.709 562.986 c b 128.83 567.715 m 128.83 568.854 129.753 569.776 130.892 569.776 c 132.03 569.776 132.953 568.854 132.953 567.715 c 132.953 566.577 132.03 565.654 130.892 565.654 c 129.753 565.654 128.83 566.577 128.83 567.715 c b 128.951 572.687 m 128.951 573.825 129.874 574.748 131.013 574.748 c 132.151 574.748 133.074 573.825 133.074 572.687 c 133.074 571.548 132.151 570.625 131.013 570.625 c 129.874 570.625 128.951 571.548 128.951 572.687 c b 128.709 580.325 m 128.709 581.464 129.632 582.387 130.77 582.387 c 131.909 582.387 132.831 581.464 132.831 580.325 c 132.831 579.187 131.909 578.264 130.77 578.264 c 129.632 578.264 128.709 579.187 128.709 580.325 c b 128.814 583.842 m 128.814 584.98 129.737 585.903 130.875 585.903 c 132.014 585.903 132.937 584.98 132.937 583.842 c 132.937 582.703 132.014 581.78 130.875 581.78 c 129.737 581.78 128.814 582.703 128.814 583.842 c b 122.535 532.508 m 139.025 532.508 l S 189.091 463.077 m 189.091 464.215 190.014 465.138 191.153 465.138 c 192.291 465.138 193.214 464.215 193.214 463.077 c 193.214 461.938 192.291 461.016 191.153 461.016 c 190.014 461.016 189.091 461.938 189.091 463.077 c b 182.665 484.174 m 182.665 485.313 183.588 486.235 184.726 486.235 c 185.865 486.235 186.788 485.313 186.788 484.174 c 186.788 483.036 185.865 482.113 184.726 482.113 c 183.588 482.113 182.665 483.036 182.665 484.174 c b 182.786 487.69 m 182.786 488.829 183.709 489.752 184.848 489.752 c 185.986 489.752 186.909 488.829 186.909 487.69 c 186.909 486.552 185.986 485.629 184.848 485.629 c 183.709 485.629 182.786 486.552 182.786 487.69 c b 189.213 486.842 m 189.213 487.98 190.136 488.903 191.274 488.903 c 192.412 488.903 193.335 487.98 193.335 486.842 c 193.335 485.703 192.412 484.78 191.274 484.78 c 190.136 484.78 189.213 485.703 189.213 486.842 c b 189.213 485.023 m 189.213 486.161 190.136 487.084 191.274 487.084 c 192.412 487.084 193.335 486.161 193.335 485.023 c 193.335 483.885 192.412 482.962 191.274 482.962 c 190.136 482.962 189.213 483.885 189.213 485.023 c b 189.213 481.628 m 189.213 482.766 190.136 483.689 191.274 483.689 c 192.412 483.689 193.335 482.766 193.335 481.628 c 193.335 480.49 192.412 479.567 191.274 479.567 c 190.136 479.567 189.213 480.49 189.213 481.628 c b 195.76 486.235 m 195.76 487.374 196.683 488.297 197.821 488.297 c 198.96 488.297 199.883 487.374 199.883 486.235 c 199.883 485.097 198.96 484.174 197.821 484.174 c 196.683 484.174 195.76 485.097 195.76 486.235 c b 196.245 499.452 m 196.245 500.59 197.168 501.513 198.306 501.513 c 199.445 501.513 200.368 500.59 200.368 499.452 c 200.368 498.313 199.445 497.39 198.306 497.39 c 197.168 497.39 196.245 498.313 196.245 499.452 c b 189.334 497.148 m 189.334 498.286 190.257 499.209 191.395 499.209 c 192.534 499.209 193.456 498.286 193.456 497.148 c 193.456 496.01 192.534 495.087 191.395 495.087 c 190.257 495.087 189.334 496.01 189.334 497.148 c b 189.35 501.877 m 189.35 503.015 190.273 503.938 191.411 503.938 c 192.55 503.938 193.473 503.015 193.473 501.877 c 193.473 500.738 192.55 499.815 191.411 499.815 c 190.273 499.815 189.35 500.738 189.35 501.877 c b 189.229 508.303 m 189.229 509.441 190.152 510.364 191.29 510.364 c 192.429 510.364 193.351 509.441 193.351 508.303 c 193.351 507.165 192.429 506.242 191.29 506.242 c 190.152 506.242 189.229 507.165 189.229 508.303 c b 189.471 520.428 m 189.471 521.566 190.394 522.489 191.533 522.489 c 192.671 522.489 193.594 521.566 193.594 520.428 c 193.594 519.289 192.671 518.367 191.533 518.367 c 190.394 518.367 189.471 519.289 189.471 520.428 c b 195.898 519.943 m 195.898 521.081 196.821 522.004 197.959 522.004 c 199.097 522.004 200.02 521.081 200.02 519.943 c 200.02 518.805 199.097 517.882 197.959 517.882 c 196.821 517.882 195.898 518.805 195.898 519.943 c b 189.229 539.828 m 189.229 540.966 190.152 541.889 191.29 541.889 c 192.429 541.889 193.351 540.966 193.351 539.828 c 193.351 538.689 192.429 537.766 191.29 537.766 c 190.152 537.766 189.229 538.689 189.229 539.828 c b 189.229 560.44 m 189.229 561.579 190.152 562.502 191.29 562.502 c 192.429 562.502 193.351 561.579 193.351 560.44 c 193.351 559.302 192.429 558.379 191.29 558.379 c 190.152 558.379 189.229 559.302 189.229 560.44 c b 249.959 470.473 m 249.959 471.611 250.882 472.534 252.02 472.534 c 253.158 472.534 254.081 471.611 254.081 470.473 c 254.081 469.335 253.158 468.412 252.02 468.412 c 250.882 468.412 249.959 469.335 249.959 470.473 c b 243.29 486.114 m 243.29 487.253 244.213 488.175 245.351 488.175 c 246.49 488.175 247.413 487.253 247.413 486.114 c 247.413 484.976 246.49 484.053 245.351 484.053 c 244.213 484.053 243.29 484.976 243.29 486.114 c b 249.838 484.659 m 249.838 485.797 250.76 486.72 251.899 486.72 c 253.037 486.72 253.96 485.797 253.96 484.659 c 253.96 483.521 253.037 482.598 251.899 482.598 c 250.76 482.598 249.838 483.521 249.838 484.659 c b 256.143 483.689 m 256.143 484.828 257.065 485.75 258.204 485.75 c 259.342 485.75 260.265 484.828 260.265 483.689 c 260.265 482.551 259.342 481.628 258.204 481.628 c 257.065 481.628 256.143 482.551 256.143 483.689 c b 249.838 490.6 m 249.838 491.739 250.76 492.662 251.899 492.662 c 253.037 492.662 253.96 491.739 253.96 490.6 c 253.96 489.462 253.037 488.539 251.899 488.539 c 250.76 488.539 249.838 489.462 249.838 490.6 c b 243.29 498.967 m 243.29 500.105 244.213 501.028 245.351 501.028 c 246.49 501.028 247.413 500.105 247.413 498.967 c 247.413 497.828 246.49 496.905 245.351 496.905 c 244.213 496.905 243.29 497.828 243.29 498.967 c b 249.716 500.422 m 249.716 501.56 250.639 502.483 251.778 502.483 c 252.916 502.483 253.839 501.56 253.839 500.422 c 253.839 499.283 252.916 498.36 251.778 498.36 c 250.639 498.36 249.716 499.283 249.716 500.422 c b 239.783 488.252 m 262.578 488.252 l 178.674 497.588 m 201.832 497.588 l S 249.838 525.399 m 249.838 526.537 250.76 527.46 251.899 527.46 c 253.037 527.46 253.96 526.537 253.96 525.399 c 253.96 524.261 253.037 523.338 251.899 523.338 c 250.76 523.338 249.838 524.261 249.838 525.399 c b 129.082 587.677 m 129.082 590.223 l 143.754 590.223 l 173.581 590.345 m 188.737 590.345 l 188.737 587.677 l 129.082 597.386 m 129.082 599.932 l 177.703 599.932 l 191.162 587.677 m 191.162 590.223 l 214.927 590.223 l 226.81 590.466 m 250.817 590.466 l 250.817 587.677 l 203.893 600.021 m 250.938 600.021 l 250.938 597.111 l S BT 9.7 0 0 9.7 146.914 587.475 Tm /GS3 gs (p=0.02)Tj 3.2491 1.0333 TD (p=0.01)Tj 7.76 0 0 7.76 216.276 587.719 Tm (NS)Tj /F3 1 Tf 7.97 0 0 7.97 58.28 416.409 Tm 0 g /GS2 gs [(Fig.)-327.1(3.)-331.2(\261)-332.2(Intercellul)9.2(ar)-407.5(adhesion)-397.3(molecule)-397.8(\(ICAM\)-1)-395.6(expressed)-400.3(as)-401.4(per)14.3(-)]TJ 0 -1.0599 TD [(centage)-268.1(of)-280.5(the)-272.5(total)-273.1(number)-271.4(of)-273.4(the)-279.6(vessel)-267.5(marker)35.3(,)-276.4(EN4+,)-275.2(vessels)-266.6(\(a\))-277.6(and)]TJ 0 -1.067 TD [(as)-280.5(number)-278.5(of)-287.6(ICAM-)11(1+)-282.4(vessels)-280.9(per)-284.5(square)-278.4(millimetre)-281.3(submucosa)-273.4(\(b\))-285.7(in)]TJ 0 -1.0599 TD [(the)-229.8(biopsies)-231.2(of)-230.7(severe)-227.6(asthmatics,)-226.5(mild)-231.4(asthmatics)-227.6(and)-234.5(healthy)-229.9(nonatop)13.3(ic)]TJ T* [(control)-224(subjects.)-222(Compariso)11.2(n)-225.5(between)-221.9(groups)-223.5(was)-224(by)-230.5(Mann-Wh)12.5(itney)-228(U-)]TJ 0 -1.067 TD [(test)-501.6(with)-496.8(Bonferroni)]TJ /F10 1 Tf 8.4504 0 TD (')Tj /F3 1 Tf 0.2205 0 TD [(s)-497(correction.)-492.1(The)-500.4(horizontal)-489.3(lines)-499.5(represent)-494.1(the)]TJ -8.6709 -1.0599 TD [(median)-329.5(values.)]TJ 5.579 0 0 5.579 108.85 365.612 Tm (NS)Tj 7.97 0 0 7.97 115.994 365.612 Tm [(:)-327.5(nonsignificant.)]TJ ET 0.49 w /GS4 gs 350.381 761.895 m 350.381 621.756 l 350.381 618.667 m 536.091 618.667 l 350.442 622.001 m 347.196 622.001 l 350.442 649.88 m 347.196 649.88 l 350.442 678.038 m 347.196 678.038 l 350.442 705.917 m 347.196 705.917 l 350.442 734.165 m 347.196 734.165 l 350.442 761.648 m 347.196 761.648 l 381.557 618.667 m 381.557 615.972 l 443.297 618.667 m 443.297 615.972 l 504.759 618.667 m 504.759 615.972 l S 0 0 0 1 k 373.476 631.612 m 373.476 632.762 374.408 633.695 375.558 633.695 c 376.708 633.695 377.641 632.762 377.641 631.612 c 377.641 630.462 376.708 629.53 375.558 629.53 c 374.408 629.53 373.476 630.462 373.476 631.612 c b BT /F14 1 Tf 9.8 0 0 9.8 311.623 765.663 Tm /GS3 gs -0.0038 Tc (a\))Tj ET /GS4 gs 350.659 588.853 m 350.659 448.714 l 350.659 445.625 m 536.369 445.625 l 350.72 448.959 m 347.474 448.959 l 350.72 480.12 m 347.474 480.12 l 350.72 495.501 m 347.474 495.501 l 350.72 510.742 m 347.474 510.742 l 350.72 541.765 m 347.474 541.765 l 350.72 572.51 m 347.474 572.51 l 350.72 588.606 m 347.474 588.606 l 381.835 445.625 m 381.835 442.93 l 443.575 445.625 m 443.575 442.93 l 505.037 445.625 m 505.037 442.93 l S BT 9.8 0 0 9.8 492.408 436.346 Tm /GS3 gs 0 Tc (Control)Tj -0.1824 -1 TD (subjects)Tj -5.5647 1.023 TD (Mild)Tj -1.2074 -1 TD (asthmatics)Tj -5.6128 0.977 TD [(Se)30(v)25(ere)]TJ -0.6875 -1 TD (asthmatics)Tj ET /GS4 gs 385.268 459.182 m 385.268 460.333 386.201 461.265 387.351 461.265 c 388.501 461.265 389.433 460.333 389.433 459.182 c 389.433 458.032 388.501 457.1 387.351 457.1 c 386.201 457.1 385.268 458.032 385.268 459.182 c b BT 9.8 0 0 9.8 311.901 592.621 Tm /GS3 gs -0.0038 Tc (b\))Tj 2.8925 -14.9775 TD 0 Tc (0)Tj -0.1029 17.6193 TD (0)Tj -0.4375 2.8908 TD -0.0116 Tc (10)Tj 0 2.8875 TD (20)Tj 0 2.8216 TD (30)Tj -0.0125 2.8433 TD 0 Tc (40)Tj 0 2.8341 TD (50)Tj ET /GS4 gs 380.213 625.12 m 380.213 626.27 381.146 627.202 382.296 627.202 c 383.446 627.202 384.378 626.27 384.378 625.12 c 384.378 623.97 383.446 623.037 382.296 623.037 c 381.146 623.037 380.213 623.97 380.213 625.12 c b 387.073 627.937 m 387.073 629.087 388.006 630.02 389.156 630.02 c 390.306 630.02 391.238 629.087 391.238 627.937 c 391.238 626.787 390.306 625.855 389.156 625.855 c 388.006 625.855 387.073 626.787 387.073 627.937 c b 380.091 630.265 m 380.091 631.415 381.023 632.347 382.173 632.347 c 383.323 632.347 384.256 631.415 384.256 630.265 c 384.256 629.114 383.323 628.182 382.173 628.182 c 381.023 628.182 380.091 629.114 380.091 630.265 c b 380.213 632.592 m 380.213 633.742 381.146 634.674 382.296 634.674 c 383.446 634.674 384.378 633.742 384.378 632.592 c 384.378 631.442 383.446 630.51 382.296 630.51 c 381.146 630.51 380.213 631.442 380.213 632.592 c b 387.196 640.554 m 387.196 641.705 388.128 642.637 389.278 642.637 c 390.428 642.637 391.361 641.705 391.361 640.554 c 391.361 639.404 390.428 638.472 389.278 638.472 c 388.128 638.472 387.196 639.404 387.196 640.554 c b 380.458 642.392 m 380.458 643.542 381.391 644.474 382.541 644.474 c 383.691 644.474 384.623 643.542 384.623 642.392 c 384.623 641.242 383.691 640.31 382.541 640.31 c 381.391 640.31 380.458 641.242 380.458 642.392 c b 373.843 640.8 m 373.843 641.95 374.776 642.882 375.926 642.882 c 377.076 642.882 378.008 641.95 378.008 640.8 c 378.008 639.649 377.076 638.717 375.926 638.717 c 374.776 638.717 373.843 639.649 373.843 640.8 c b 380.213 652.682 m 380.213 653.832 381.146 654.764 382.296 654.764 c 383.446 654.764 384.378 653.832 384.378 652.682 c 384.378 651.532 383.446 650.6 382.296 650.6 c 381.146 650.6 380.213 651.532 380.213 652.682 c b 380.336 658.562 m 380.336 659.712 381.268 660.644 382.418 660.644 c 383.568 660.644 384.501 659.712 384.501 658.562 c 384.501 657.412 383.568 656.479 382.418 656.479 c 381.268 656.479 380.336 657.412 380.336 658.562 c b 380.458 666.402 m 380.458 667.552 381.391 668.484 382.541 668.484 c 383.691 668.484 384.623 667.552 384.623 666.402 c 384.623 665.252 383.691 664.319 382.541 664.319 c 381.391 664.319 380.458 665.252 380.458 666.402 c b 380.458 674.487 m 380.458 675.637 381.391 676.569 382.541 676.569 c 383.691 676.569 384.623 675.637 384.623 674.487 c 384.623 673.337 383.691 672.404 382.541 672.404 c 381.391 672.404 380.458 673.337 380.458 674.487 c b 387.318 673.752 m 387.318 674.902 388.251 675.834 389.401 675.834 c 390.551 675.834 391.483 674.902 391.483 673.752 c 391.483 672.602 390.551 671.669 389.401 671.669 c 388.251 671.669 387.318 672.602 387.318 673.752 c b 380.581 685.022 m 380.581 686.172 381.513 687.104 382.663 687.104 c 383.813 687.104 384.746 686.172 384.746 685.022 c 384.746 683.872 383.813 682.939 382.663 682.939 c 381.513 682.939 380.581 683.872 380.581 685.022 c b 380.581 704.744 m 380.581 705.894 381.513 706.827 382.663 706.827 c 383.813 706.827 384.746 705.894 384.746 704.744 c 384.746 703.594 383.813 702.661 382.663 702.661 c 381.513 702.661 380.581 703.594 380.581 704.744 c b 443.668 669.219 m 443.668 670.37 444.6 671.302 445.751 671.302 c 446.901 671.302 447.833 670.37 447.833 669.219 c 447.833 668.069 446.901 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[(3.)-1766.5(W)32.8(ilson)-382.8(JW)82.7(,)-382.3(Xun)-381.2(Li,)-380.8(Pain)-384.4(MCF)73.4(.)-382.3(Lack)-386.7(of)-379.3(airway)-388.8(disten-)]TJ 2.5165 -1.1065 TD [(sibility)-388.4(in)-380.8(asthma.)]TJ /F4 1 Tf 7.714 0 TD [(Am)-385.2(Rev)-380.9(Respir)-390.7(Dis)]TJ /F3 1 Tf 8.2957 0 TD [(1993;)-385.5(148:)-379.7(806\261)]TJ -16.0096 -1.1128 TD (809.)Tj -2.5165 -1.1128 TD [(4.)-1766.5(Charan)-577.6(NB,)-572.6(Baile)-581.9(EM,)-575.1(Pare)]TJ 14.5111 0.0443 TD (\302)Tj 0.9548 -0.0443 TD [(PD.)-572.8(Bronchial)-580.8(vascular)]TJ -12.9493 -1.1065 TD [(congestion)-518.9(and)-523.1(angiogenesis.)]TJ /F4 1 Tf 12.6774 0 TD [(Eur)-521.6(Respir)-523.5(J)]TJ /F3 1 Tf 6.0763 0 TD [(1997;)-524.6(10:)]TJ -18.7538 -1.1128 TD [(1)32.1(173\2611)34.2(180.)]TJ 25.4813 73.5671 TD [(5.)-1766.5(Kuwano)-395.3(K,)-391.4(Bosken)-394.6(CH,)-395.6(Pare)]TJ 14.4352 0.0379 TD (\302)Tj 0.7651 -0.0379 TD [(PD,)-395.8(Bai)-391(TR,)-395.8(W)26.5(iggs)-393.6(BR,)]TJ -12.6838 -1.1191 TD [(Hogg)-285.8(JC.)-292.3(Small)-293.3(airways)-294.7(dimensions)-288.2(in)-292.2(asthma)-292.8(and)-289.1(chro-)]TJ 0 -1.1128 TD [(nic)-436.9(obstructive)-439.5(pulmonary)-436.7(disease.)]TJ /F4 1 Tf 14.7513 0 TD [(Am)-435.7(Rev)-437.8(Respir)-441.3(Dis)]TJ /F3 1 Tf -14.7513 -1.1192 TD [(1993;)-334.9(148:)-329.1(1220\2611225.)]TJ -2.5165 -1.1065 TD [(6.)-1766.5(Carroll)-285.8(NG,)-280.4(Cooke)-280.1(C,)-279(James)-285.8(AL.)-280.6(Bronchial)-283.6(blood)-278.1(vessel)]TJ 2.5165 -1.1192 TD [(dimensions)-288.2(in)-279.6(asthma.)]TJ /F4 1 Tf 9.1935 0 TD [(Am)-284(J)-283.8(Respir)-283.2(Crit)-286.8(Car)27.2(e)-283.8(Med)]TJ /F3 1 Tf 11.3559 0 TD (1997;)Tj -20.5495 -1.1128 TD [(155:)-335.5(689\261695.)]TJ -2.5165 -1.1128 TD [(7.)-1766.5(Smith)-287.2(CH,)-288.1(Barker)-287.7(JNW)86.3(,)-287.4(Lee)-286(TH.)-286.9(Adhesion)-287.1(molecules)-287.1(in)]TJ 2.5165 -1.1192 TD [(aller)9.9(gic)-506.5(inflammation.)]TJ /F4 1 Tf 9.5097 0 TD [(Am)-505.3(Rev)-501(Respir)-504.5(Dis)]TJ /F3 1 Tf 8.7699 0 TD [(1993;)-499.3(148:)]TJ -18.2795 -1.1128 TD (S75\261S78.)Tj -2.5165 -1.1128 TD [(8.)-1766.5(Kunkel)-217.4(EJ,)-215.2(Jung)-220.5(U,)-214.3(Bullard)-221.6(DC,)]TJ /F4 1 Tf 15.4405 0 TD [(et)-216.1(al)]TJ /F3 1 Tf 1.6945 0 TD [(.)-217.9(Absence)-215.2(of)-221.3(trauma-)]TJ -14.6186 -1.1128 TD [(induced)-453.6(leukocyte)-461.1(rolling)-453.5(in)-456.6(mice)-457(deficient)-458.1(in)-456.6(both)-455.6(P-)]TJ 0 -1.1192 TD [(selectin)-250.2(and)-251.2(intercellular)-252.9(adhesion)-245.6(molecule)-254.8(1.)]TJ /F4 1 Tf 18.583 0 TD [(J)-252.2(Exp)-248.3(Med)]TJ /F3 1 Tf -18.583 -1.1128 TD [(1996;)-334.9(183:)-329.1(57\26165.)]TJ -2.5165 -1.1128 TD [(9.)-1766.5(Montefort)-261.8(S,)-266.5(Roche)-261(WR,)-265.1(Howarth)-265.4(PH,)]TJ /F4 1 Tf 18.2795 0 TD [(et)-266.7(al)]TJ /F3 1 Tf 1.7451 0 TD [(.)-262.2(Intercellular)]TJ -17.5081 -1.1128 TD [(adhesion)-233(molecule-1)-235.8(\(ICAM-1\))-236.1(and)-232.2(endothelial)-230.9(leucocyte)]TJ 0 -1.1192 TD [(adhesion)-365.8(molecule-1)-374.9(\(ELAM-1\))-363.8(expression)-373.2(in)-368.1(the)-367.4(bron-)]TJ 0 -1.1128 TD [(chial)-298.9(mucosa)-290.4(of)-297.1(normal)-299.1(and)-295.4(asthmatic)-295.8(subjects.)]TJ /F4 1 Tf 19.2849 0 TD [(Eur)-294(Res-)]TJ -19.2849 -1.1192 TD [(pir)-330.9(J)]TJ /F3 1 Tf 2.2573 0 TD [(1992;)-334.9(5:)-336.5(815\261823.)]TJ -4.7738 -1.1128 TD [(10.)-1266.5(Montefort)-413.5(S,)-405.7(Gratziou)-415.5(C,)-405.5(Goulding)-415(D,)]TJ /F4 1 Tf 18.8865 0 TD [(et)-405.8(al)]TJ /F3 1 Tf 1.8842 0 TD [(.)-407.6(Bronchial)]TJ -18.2542 -1.1128 TD [(biopsy)-367.3(evidence)-370.5(for)-373.5(leukocyte)-366.2(infiltration)-376.6(and)-365(upregula-)]TJ 0 -1.1192 TD [(tion)-274.6(of)-271.8(leukocyte-endothelial)-281.4(adhesion)-270.9(molecules)-280.8(6)-271.4(hours)]TJ 0 -1.1128 TD [(after)-247.9(local)-248.4(allergen)-241.3(challenge)-245.9(of)-246.6(sensitized)-250.4(airways.)]TJ /F4 1 Tf 20.4293 0 TD [(J)-245.9(Clin)]TJ -20.4293 -1.1192 TD (lnvest)Tj /F3 1 Tf 2.6367 0 TD [(1994;)-328.6(93:)-336(141)33.1(1\2611421.)]TJ -5.1532 -1.1065 TD [(1)25.8(1)0(.)-1292.3(V)54.1(rugt)-298.5(B,)-291.6(W)32.8(ilson)-294.3(S,)-291.8(Underwood)-299.9(J,)]TJ /F4 1 Tf 16.4143 0 TD [(et)-298.3(al)]TJ /F3 1 Tf 1.7767 0 TD [(.)-293.8(Mucosal)-291.1(inflam-)]TJ -15.6745 -1.1192 TD [(mation)-427(in)-418.7(severe)-428.4(glucocorticoid-dependent)-430.2(asthma.)]TJ /F4 1 Tf 21.3272 0 TD (Eur)Tj -21.3272 -1.1128 TD [(Respir)-333.8(J)]TJ /F3 1 Tf 3.6863 0 TD [(1999;)-334.9(13:)-329.7(1245\2611252.)]TJ -6.2028 -1.1128 TD [(12.)-1266.5(Xun)-223.1(Li,)-222.7(W)32.8(ilson)-224.7(JW)82.7(.)-217.9(Increased)-225.3(vascularity)-224.3(of)-227.6(the)-221.9(bronchial)]TJ 2.5165 -1.1192 TD [(mucosa)-227.2(in)-222.7(mild)-225.9(asthma.)]TJ /F4 1 Tf 9.5539 0 TD [(Am)-227.1(J)-220.6(Respir)-226.3(Crit)-223.6(Car)27.2(e)-220.6(Med)]TJ /F3 1 Tf 10.9955 0 TD (1997;)Tj -20.5495 -1.1128 TD [(156:)-335.5(229\261233.)]TJ -2.5165 -1.1128 TD [(13.)-1266.5(American)-376.8(Thoracic)-381.5(Society)63.9(.)-376(Standards)-377.5(for)-379.8(the)-373.7(diagnosis)]TJ 2.5165 -1.1128 TD [(and)-346(care)-352.3(of)-347.7(patients)-345.2(with)-347.5(chronic)-353.4(obstructive)-351(pulmonary)]TJ 0 -1.1192 TD [(disease)-392.5(\(COPD\))-400.8(and)-390.3(asthma.)]TJ /F4 1 Tf 12.2159 0 TD [(Am)-397.8(Rev)-393.5(Respir)-390.7(Dis)]TJ /F3 1 Tf 8.3336 0 TD (1987;)Tj -20.5495 -1.1128 TD [(136:)-335.5(225\261244.)]TJ -2.5165 -1.1128 TD [(14.)-1266.5(W)70.8(oolcock)-592.7(AJ.)-600.7(Management)-599.6(of)-600.6(asthma)-596.3(in)-595.7(adults.)]TJ /F4 1 Tf 24.242 0 TD (In)Tj /F3 1 Tf 0.822 0 TD (:)Tj -22.5475 -1.1192 TD [(Barnes)-275.2(PJ,)-273.6(Rodger)-280.6(IW)83(,)-274.8(Thomson)-274.9(NC,)-275.5(eds.)-274.2(Asthma,)-280.2(basic)]TJ 0 -1.1128 TD [(mechanisms)-268.3(and)-257.5(clinical)-270.3(management.)-262.3(2nd)-264(Edn.)-264.1(London,)]TJ 0 -1.1192 TD [(UK,)-330.9(Academic)-337.7(Press)-334.3(Ltd,)-336(1992;)-334.9(679\261699.)]TJ -2.5165 -1.1065 TD [(15.)-1266.5(R)33.7(yan)-333.4(G,)-334.5(Latimer)-335.9(KM,)-334.7(Dolovich)-332.5(J,)-332.4(Harg)10.6(reave)-332.9(FE.)-332.7(Bron-)]TJ 2.5165 -1.1192 TD [(chial)-305.3(responsiveness)-310.1(to)-304.9(histamine:)-310.5(relationship)-314.9(to)-304.9(diurnal)]TJ 0 -1.1128 TD [(variation)-350.8(of)-347.7(peak)-345.3(flow)-352.4(rate,)-345.2(improvement)-351.2(after)-349.1(broncho-)]TJ 0 -1.1192 TD [(dilator)27.3(,)-331.7(and)-333.4(airway)-338.2(calibre.)]TJ /F4 1 Tf 11.1726 0 TD (Thorax)Tj /F3 1 Tf 3.2057 0 TD [(1982;)-334.9(37:)-336(423\261429.)]TJ -16.8948 -1.1065 TD [(16.)-1266.5(Quanjer)-382(PH.)-383.1(Standardized)-389.3(lung)-379.7(function)-387.1(testing.)-380.5(Report)]TJ 2.5165 -1.1192 TD [(W)70.8(orking)-392.3(Party)-391.2(Standardization)-392.5(of)-392(Lung)-393.5(Function)-391.1(T)60.2(ests,)]TJ 0 -1.1128 TD [(European)-499.4(Community)-501.8(for)-499.9(Coal)-498(and)-497.8(Steel.)]TJ /F4 1 Tf 18.2163 0 TD [(Bull)-499.4(Eur)28.5(op)]TJ -18.2163 -1.1192 TD 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[(ding)-620(techniques)-619.8(for)-620.1(small)-618.4(mucosal)-621.1(biopsies.)]TJ /F4 1 Tf 19.797 0 TD (Biotech)Tj -19.797 -1.1128 TD (Histochem)Tj /F3 1 Tf 4.5715 0 TD [(1993;)-334.9(68:)-329.6(271\261280.)]TJ -7.088 -1.1128 TD [(19.)-1266.5(Laitinen)-221.1(LA,)-223.6(Laitinen)-221.1(MA,)-220.8(W)26.5(iddicombe)-222.9(JG.)-221.4(Dose-related)]TJ 2.5165 -1.1192 TD [(effects)-241.5(of)-252.9(pharmacological)-254.1(mediators)-256.7(on)-245.6(tracheal)-260(vascular)]TJ 0 -1.1128 TD [(resistance)-337.2(in)-336.5(dogs.)]TJ /F4 1 Tf 7.7898 0 TD [(Br)-332.5(J)-334.4(Pharmacol)]TJ /F3 1 Tf 6.8414 0 TD [(1987;)-334.9(92:)-329.7(703\261709.)]TJ -17.1477 -1.1128 TD [(20.)-1266.5(Church)-457.6(MK,)-454.8(Levi-Schaffer)-449.7(F)77.8(.)-458.2(The)-457(human)-454.4(mast)-452.1(cell.)]TJ /F4 1 Tf 24.8996 0 TD (J)Tj -22.3831 -1.1128 TD [(Aller)31.3(gy)-333.9(Clin)-336.2(Immunol)]TJ /F3 1 Tf 9.1113 0 TD [(1997;)-334.9(99:)-329.7(155\261160.)]TJ -11.6278 -1.1128 TD 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