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[(atrop)-8.2(ine)-170.1([13])-177(have)-180.3(a)-171.9(higher)-174(BAL)-180.1(return)-175.6(than)-176.6(untre)-8.2(ated)-177(con-)]TJ 0 -1.0471 TD [(trol)-396.4(subje)-7.8(cts)-393.2(but)-397.3(this)-396(has)-390.8(not)-397.3(been)-396.5(formall)-11.1(y)-393.4(assessed)-399(in)]TJ 0 -1.0528 TD [(children.)-281.1(Effective)-270.5(topi)-7.8(cal)-272.9(airway)-280.7(anaesthe)-11.3(sia)-273.7(is)-272.6(achiev)-8.2(ed)]TJ 0 -1.0471 TD [(with)-250.7(1\261)-7.2(4%)-248.6(lignocaine)-260.7(in)-248.6(the)-249.8(upper)-256.2(airways)-254.2(and)-253.1(0.5\2612.0%)]TJ 0 -1.0528 TD [(lignoc)-9.7(aine)-154.2(in)-151.9(the)-153(lower)-160.1(airways,)-157.9(the)-153(tota)-8.2(l)-151.1(dose)-152.6(of)-151.1(lign)-7.8(ocaine)]TJ 0 -1.0471 TD [(being)-336.7(limit)-10.8(ed)-332(to)-339.7(a)-331.2(maxi)-9(mum)-338.6(of)-333.2(7)-336.5(m)0(g)]TJ /F10 1 Tf 15.1767 0.1992 TD (.)Tj /F4 1 Tf 0.2731 -0.1992 TD (kg)Tj 7.472 0 0 6.641 222.179 341.291 Tm (-1)Tj 9.963 0 0 9.963 231.647 336.869 Tm ([14].)Tj /F8 1 Tf -17.4017 -2.225 TD [(Locati)-9(on)-331.6(of)-339.7(the)-335.1(BAL)]TJ /F4 1 Tf 1.0015 -1.5478 TD [(Us)16.1(ua)15.1(l)13.9(l)0(y)-413.6(B)0(A)12.6(L)-420.3(is)-414.8(ca)20.4(rr)18(ie)18.4(d)-421.9(o)0(u)15.5(t)-418.6(in)-413.6(th)18.9(e)-422.3(m)0(o)12.4(s)0(t)17.7(-)0(a)19.2(f)20.4(fe)13.5(ct)18.5(ed)-411.7(ar)19.2(ea)]TJ -1.0015 -1.0527 TD [(\(i)17.3(de)15.1(nt)18.9(if)17.3(ie)18.4(d)-211.3(r)0(a)13.5(d)0(i)18.9(o)0(l)18.9(o)0(g)15.5(i)0(c)18.4(a)0(l)18.4(l)0(y)-203.1(a)0(n)15.1(d)0(/)18.9(o)0(r)-202.3(e)0(n)15.1(d)0(o)21.2(s)0(c)13.9(o)0(p)21.2(i)0(c)12.8(a)0(l)18.4(l)0(y)18.9(\))0(.)-201.9(I)0(n)-208(d)0(i)18.9(f)20.4(fu)13.9(se)]TJ 25.1977 48.4664 TD [(lu)13.2(ng)-451.1(di)18.8(se)19.6(as)13.9(e)-462.1(t)0(h)18.9(e)-462.1(ri)17.3(gh)21.2(t)-464.1(m)0(i)15.7(d)0(d)15.5(l)13.9(e)-462.1(l)0(o)13.2(b)0(e)-451.5(i)0(s)-449(t)0(h)13.2(e)-462.1(pr)19.6(ef)19.2(er)13.5(re)19.2(d)-461.7(s)0(i)17.7(t)0(e)]TJ 0 -1.0641 TD [(be)15.1(ca)14.7(us)20(e)-547.5(t)13.9(hi)13.2(s)-542.6(a)0(r)13.5(e)0(a)-531.6(o)0(f)25.3(f)0(e)19.2(r)0(s)-539.2(b)0(e)15.1(t)13.9(te)12.8(r)-543(f)0(l)17.3(u)0(i)18.9(d)-547.1(re)19.2(co)15.1(ve)20.8(ry)-538([1)13.9(5].)-544.1(In)]TJ 0 -1.0698 TD [(infan)-8.2(ts)-232.7(it)-233.9(is)-227(often)-235.8(easie)-8.6(r)-230(t)0(o)-231.5(perfor)-9.8(m)-231.5(BAL)-231.3(in)-231.5(the)-232.7(right)-237.8(lower)]TJ 0 -1.0641 TD [(lobe.)-347.7(If)-340.5(BAL)-345.1(and)-338.4(trans)-9.4(bronchi)-9.7(al)-340.1(biopsies)-349.4(are)-340.4(planne)-7.8(d)-336.5(i)0(n)]TJ 0 -1.0698 TD [(the)-340.8(same)-338.2(patient,)-343.7(BAL)-333.7(shoul)-7.4(d)-336.5(b)0(e)-332(perfor)-9.8(med)-335.9(first.)]TJ /F8 1 Tf 0 -3.0957 TD [(V)103.2(olum)-9.4(e)-331.2(o)0(f)-339.6(lavage)-337.8(solution)]TJ /F4 1 Tf 1.0015 -1.7527 TD [(BA)12.6(L)-209.7(i)0(s)-198.6(c)0(a)14.7(r)0(r)18(i)0(e)18.4(d)-211.3(ou)15.5(t)-208(u)0(s)20(i)0(n)18.9(g)-211.3(no)15.5(rm)16.5(al)-197.8(st)17.7(er)13.5(il)16.5(e)-206(s)0(a)13.9(l)0(i)16.5(n)0(e)-201.1(p)0(r)19.6(e)0(v)20.8(i)0(o)13.2(u)0(s)20(l)0(y)]TJ -1.0015 -1.0641 TD [(wa)16.9(rm)10.8(ed)-314.9(to)-311.2(bo)15.5(dy)-314.5(te)18.4(mp)18.1(er)13.5(at)18.4(ur)19.6(e)-325.5(\()0(3)13.9(7)]TJ /F5 1 Tf 13.0883 0 TD (8)Tj /F4 1 Tf 0.3756 0 TD [(C\))14.9(.)]TJ -12.4623 -1.0527 TD [(Li)14.7(mi)15.7(te)18.4(d)-347.9(i)0(n)18.9(f)0(o)13.9(r)0(m)16.5(a)0(t)18.5(i)0(o)18.8(n)-347.9(ex)15.1(is)17.7(ts)-340.8(on)-337.3(th)18.9(e)-348.3(a)0(m)17.7(o)0(u)15.5(n)0(t)-339.6(o)0(f)-327.5(f)0(l)17.3(u)0(i)18.9(d)-347.9(an)15.1(d)]TJ -1.0015 -1.0641 TD [(th)13.2(e)-280(n)0(u)21.2(m)0(b)18.1(e)0(r)-276.7(o)0(f)-270.6(a)0(l)18.4(i)0(q)18.8(u)0(o)15.5(t)0(s)-272.6(t)0(h)18.9(a)0(t)-271.8(s)0(h)20(o)0(u)15.5(l)0(d)-271.4(b)0(e)-269.4(u)0(s)14.3(e)0(d)-269.4(i)0(n)-271.4(o)0(r)19.6(d)0(e)15.1(r)-281.2(to)-271.4(ob)21.2(-)]TJ 0 -1.0698 TD [(ta)12.8(in)-203.1(sa)19.6(mp)18.1(le)12.8(s)-212.5(r)0(e)19.2(p)0(r)19.6(e)0(s)14(e)0(n)20.8(t)0(a)18.4(t)0(i)16.5(v)0(e)-206.8(f)0(o)19.6(r)-212.9(th)18.9(e)-211.7(a)0(l)12.8(v)0(e)20.8(o)0(l)18.9(a)0(r)-208.4(c)0(o)20.8(m)0(p)18.1(a)0(r)13.5(t)0(m)15.7(e)0(n)20.8(t)-213.7(in)]TJ 0 -1.0641 TD [(ch)15.1(il)16.5(dr)19.6(en)-309.2(of)-304.7(di)18.9(f)14.7(f)0(e)19.2(r)0(e)19.2(n)0(t)-305.5(a)0(g)15.1(e)0(s)-304.7(a)0(n)15.1(d)-313.7(si)17.7(ze)14.7(s.)-303.9(V)114.8(a)0(r)13.5(i)0(o)18.9(u)0(s)-304.3(p)0(r)14(o)0(t)18.9(o)0(c)15.1(o)0(l)18.9(s)-314.9(fo)19.6(r)]TJ T* -0.0165 Tc [(B)-16.5(A)-3.9(L)-254.7(h)-16.5(av)-16.5(e)-246.1(b)-16.5(ee)-16.5(n)-246.1(a)-16.5(pp)-16.5(li)-16.5(ed)-16.5(.)-245.3(S)-16.5(om)-16.5(e)-249.2(u)-16.5(se)-256.7(t)-16.5(w)-1.6(o)-256.3(o)-16.5(r)-247.2(f)-16.5(ou)-16.5(r)-247.2(f)-16.5(ra)-16.5(ct)-16.5(i)5.7(o)-16.5(ns)-257.5(o)-16.5(f)]TJ 0 -1.0698 TD 0 Tc [(th)13.2(e)-308.5(s)0(a)13.9(m)0(e)-306.7(v)0(o)21.2(l)0(u)18.9(m)0(e)-306.7(\()0(1)19.6(0)0(\261)15.5(2)0(0)-303.1(m)0(L)14(\))-309.7(ir)17.3(re)13.5(sp)20(ec)14.7(ti)16.5(ve)-303.5(of)-304.7(t)13.9(h)0(e)-309.2(p)0(a)20.8(t)0(i)16.5(e)0(n)20.8(t)]TJ /F5 1 Tf 22.2045 0 TD (')Tj /F4 1 Tf 0.2106 0 TD (s)Tj -22.4151 -1.0641 TD [(bo)15.5(dy)-303.1(w)12.4(e)0(i)12.8(g)0(h)21.2(t)-310.4(an)15.1(d)-308.1(a)0(g)15.1(e)0(,)-303.2(a)0(s)-299(i)0(n)-305.5(a)0(d)20.8(u)0(l)18.9(t)0(s)-306.7([)0(1)19.6(6)0(,)-308.4(17].)-328.6(Others)-327.1(adjust)]TJ 0 -1.0698 TD [(BAL)-407.7(volum)-9.3(e)-399.5(t)0(o)-402.2(the)-403.4(chil)-8.2(d)]TJ /F5 1 Tf 10.5503 0 TD (')Tj /F4 1 Tf 0.2162 0 TD [(s)-400.3(funct)-8.2(ional)-407.3(residual)-407.1(c)-6.9(apacity)]TJ -10.7665 -1.0641 TD [(\(FR)-7.8(C\))-252.5(with)-256.4(5\26120)-253.4(mL)-253.5(fractions)-261.5(dependi)-8.5(ng)-251.9(on)-251.9(the)-255.5(patient)]TJ /F5 1 Tf 22.1988 0 TD (')Tj /F4 1 Tf 0.2162 0 TD (s)Tj -22.4151 -1.0698 TD [(size)-257.8([18].)-257(Others)-258.8([19])-250.9(adjus)-7.8(t)-253.5(BAL)-254.1(volume)-255.2(to)-254.3(body)-253.4(weight)]TJ 0 -1.0641 TD [(using)-263.5(3)-251.2(m)0(L)]TJ /F10 1 Tf 4.5467 0.1992 TD (.)Tj /F4 1 Tf 0.2731 -0.1992 TD (kg)Tj 7.472 0 0 6.641 367.313 557.915 Tm (-1)Tj 9.963 0 0 9.963 375.987 553.493 Tm [(of)-253.5(norm)-7.8(al)-254.7(sali)-9.4(ne)-252.3(divi)-7.8(ded)-253.1(into)-258.2(three)-259(equal)]TJ -6.6921 -1.0641 TD [(fract)-10.2(ions)-211.5(in)-208.8(chil)-8.2(dren)-209.9(wei)-9(ghing)]TJ /F9 1 Tf 12.0128 0 TD (<)Tj /F4 1 Tf 0.552 0 TD [(20)-212.1(kg)-206.4(an)-7.6(d)-205.6(3)-211.3(mL)]TJ /F10 1 Tf 6.1572 0.1992 TD (.)Tj /F4 1 Tf 0.2675 -0.1992 TD 0.0065 Tc (kg)Tj 7.472 0 0 6.641 508.535 547.313 Tm 0 Tc (-1)Tj 9.963 0 0 9.963 516.756 542.891 Tm [(in)-208.8(20)]TJ -20.8217 -1.0698 TD [(mL)-464(portions)-468.9(in)-459.2(chil)-8.2(dren)-460.3(wei)-9(ghing)]TJ /F9 1 Tf 14.6532 0 TD (>)Tj /F4 1 Tf 0.552 0 TD [(20)-462.5(kg)-462.5([20].)-461.9(If)-460(the)]TJ -15.2051 -1.0641 TD [(amoun)-7.4(t)-412.9(o)0(f)-412.8(instil)-9.6(led)-414.8(fluid)-414.2(is)-414.8(adjusted)-416.9(accordi)-10.5(ng)-411.3(to)-413.6(body)]TJ 0 -1.0698 TD [(wei)-9(ght)-220.9(in)-220.2(children)-229.5(aged)-220.1(3\26115)-225(yrs,)-221.7(a)-223.1(constant)-223.4(propor)-7.8(tion)-224.1(of)]TJ 0 -1.0641 TD [(the)-340.8(epitheli)-10.7(al)-334.4(lining)-336.1(flui)-9.4(d)-330.8(\(ELF\))-338.8(can)-338.8(be)-337.7(recovere)-10.9(d)-330.8([21].)]TJ 1.0015 -1.0528 TD [(Th)17.1(e)-228.8(f)0(r)18(a)0(c)20.4(t)0(i)16.5(o)0(n)15.5(s)-223.9(ar)19.2(e)-223.1(i)0(n)18.9(s)0(t)17.7(i)0(l)16.5(l)0(e)18.4(d)-228.4(us)20(in)18.8(g)-222.7(a)-223.1(sy)14.4(ri)17.3(ng)21.2(e)]TJ /F8 1 Tf 16.1669 0 TD [(vi)18.5(a)]TJ /F4 1 Tf 1.4113 0 TD [(th)18.9(e)-223.1(s)0(u)14.4(c)0(t)18.5(i)0(o)18.8(n)]TJ -18.5796 -1.0641 TD [(ch)15.1(an)15.1(ne)20.8(l)-407.2(o)0(f)-395.8(t)0(h)13.2(e)-405.2(F)11.4(F)0(B)11.6(,)-404.4(ea)20.4(ch)-400.3(in)18.8(st)17.7(il)16.5(la)18.5(ti)16.5(on)-399.9(be)20.8(in)18.8(g)-410.5(f)0(o)19.6(l)0(l)16.5(o)0(w)17.3(e)0(d)-400.3(b)0(y)]TJ T* [(su)14.4(f)20.4(f)0(i)17.3(c)0(i)18.4(e)0(n)15.1(t)-441.3(ai)18.4(r)-440.5(t)0(o)-430.7(e)0(n)20.8(s)0(u)14.4(r)0(e)-430.3(t)0(h)18.9(a)0(t)-431.1(t)0(h)18.9(e)-439.3(ch)15.1(an)20.8(ne)15.1(l)]TJ /F5 1 Tf 16.2351 0 TD (')Tj /F4 1 Tf 0.2049 0 TD 0.4401 Tc [(sd)440.1(e)455.2(a)440.1(d)11.4(s)440.1(p)454.5(a)440.1(c)460.5(e)0.8(i)440.1(s)]TJ -16.44 -1.0698 TD 0 Tc [(em)12(pt)18.9(y)73.2(.)-210.9(Th)17.1(e)-211.7(f)0(l)17.3(u)0(i)18.9(d)-217(m)13.2(a)0(y)-206.8(b)0(e)-201.1(r)0(e)13.5(c)0(o)20.8(v)0(e)15.1(r)0(e)19.2(d)-211.3(by)-206.4(ha)20.8(nd)-200.7(su)14.3(ct)18.5(io)18.8(n)-211.3(u)0(s)14.4(i)0(n)18.8(g)-211.3(a)]TJ 0 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[(\(e)19.2(xc)15.1(ep)15.1(t)-321.8(f)0(o)19.6(r)-326.7(th)18.9(e)-325.5(f)0(i)17.3(r)0(s)18.4(t)-327.5(sa)19.6(mp)18.1(le)18.4(\))-326.7(c)0(o)15.1(n)0(t)18.9(a)0(i)18.4(n)0(s)-315.7(f)0(e)19.2(w)-329.1(ep)20.8(it)16.5(he)15.1(li)22.2(al)-317.3(ce)14.7(ll)16.5(s.)]TJ /F6 1 Tf 1.7413 -3.4371 TD [(Process)-331.7(of)-338.9(recover)15.4(ed)-336.9(BAL)-340(fluid)-8.6(:)-332.4(cellu)-8.6(lar)-335.1(and)]TJ 4.5638 -1.0527 TD [(no)-6.4(ncellular)-332.3(componen)-12.6(ts)]TJ /F8 1 Tf -6.3051 -2.6006 TD [(Pr)27.3(ocess)-337.1(of)-334(the)-340.8(BAL)-334.1(fluid)]TJ /F4 1 Tf 1.0015 -1.6446 TD [(Wh)14.4(il)16.5(e)-336.9(i)13.9(t)-338.9(a)0(p)15.1(p)0(e)20.8(a)0(r)13.5(s)-332(pr)13.9(ef)19.2(er)19.2(ab)15.1(le)-328.7(to)-328.3(pr)19.6(ew)16.9(ar)13.5(m)-334(t)0(h)13.2(e)-331.2(BA)12.6(L)-334.9(s)0(o)14.3(l)0(u)18.9(-)]TJ -1.0015 -1.0528 TD [(ti)16.5(on)-234.8(to)-237.2(bo)15.5(dy)-234.8(te)18.4(mp)18.1(er)13.5(at)18.5(ur)19.6(e)-245.9(i)0(n)-237.2(o)0(r)19.6(d)0(e)15.1(r)-247.1(to)-231.5(in)13.2(cr)19.2(ea)14.7(se)-236.4(ce)20.4(ll)-239.6(yi)18.9(el)18.4(d)-245.5(a)0(n)15.1(d)]TJ 0 -1.0471 TD [(re)19.2(co)15.1(ve)15.1(ry)-276.3([1)13.9(5,)-291.4(32],)-300.2(the)-301(recover)-9.7(ed)-303.5(BAL)-305.3(samples)-304.3(shoul)-7.4(d)-302.4(b)0(e)]TJ 0 -1.0527 TD [(kept)-335.9(at)-340.1(4)]TJ /F5 1 Tf 3.5907 0 TD (8)Tj /F4 1 Tf 0.3813 0 TD [(C)-335.5(before)-344.3(analysis)-338.4(to)-339.7(optimi)-9.2(ze)-332.4(cell)-338.7(viabili)-9.6(ty)58.7(.)]TJ -2.9705 -1.0527 TD [(Be)16.1(fo)19.6(re)-219.8(as)19.6(se)13.9(ss)18.9(in)18.9(g)-222.7(c)0(e)14.7(l)0(l)16.5(u)0(l)18.9(a)0(r)-219.8(a)0(n)20.8(d)-228.4(no)21.2(nc)15.1(el)18.4(lu)18.9(la)18.4(r)-224.3(c)0(o)15.1(m)0(p)18.1(o)0(n)15.5(e)0(n)20.8(t)0(s)-221.3(o)0(f)]TJ -1.0015 -1.0471 TD [(BA)18.3(L,)-232.9(th)18.9(e)-240.2(f)0(l)17.3(u)0(i)13.2(d)-239.8(sh)20(ou)21.2(ld)-231.5(be)-235.2(fi)17.3(lt)16.5(er)19.2(ed)15.1(,)-239.4(u)0(s)20(i)0(n)18.9(g)-239.8(on)15.5(e)-240.2(l)0(a)18.4(y)0(e)20.8(r)-241.4(of)-230.7(st)17.7(er)13.5(il)16.5(e)]TJ 0 -1.0527 TD [(ga)15.1(uz)20.8(e)-223.1(t)0(o)-220.2(r)0(e)19.2(m)0(o)18.1(v)0(e)-218.2(m)0(u)18.1(c)0(o)15.1(u)0(s)-213.3(p)0(a)15.1(r)0(t)17.3(i)0(c)18.4(l)0(e)18.4(s)0(.)-218.6(M)0(i)16.9(c)0(r)19.2(o)0(b)15.5(i)0(o)18.9(l)0(o)18.9(g)0(i)18.9(c)0(a)14.7(l)-225.1(st)17.7(ud)15.5(ie)18.4(s)]TJ 0 -1.0471 TD [(sh)20(ou)15.5(ld)-225.8(be)-223.9(pe)15.1(rf)18(or)19.6(me)12(d)-234.1(o)0(n)-223.5(u)0(n)15.5(f)0(i)17.3(l)0(t)22.2(e)0(r)13.5(e)0(d)-223.9(B)0(A)12.6(L)-232.5(fl)17.3(ui)18.9(d)-234.1(a)0(s)-225.1(o)0(r)25.3(g)0(a)15.1(n)0(i)18.9(s)0(m)16.9(s)]TJ 0 -1.0527 TD [(ma)17.7(y)-325.1(b)0(e)-314.9(t)0(r)17.3(a)0(p)15.1(p)0(e)20.8(d)-325.1(in)-316.9(th)18.9(e)-325.5(m)0(u)18.1(c)0(o)15.1(u)0(s)20(.)]TJ /F8 1 Tf 0 -2.9818 TD [(Pr)27.3(ocessing)-341.7(of)-334(the)-335.1(fir)-8.2(st)-335.2(sample)]TJ /F4 1 Tf 1.0015 -1.7527 TD [(Th)17.1(er)19.2(e)-507.6(i)0(s)-494.5(n)0(o)-490.9(c)0(o)15.1(n)0(s)20(e)0(n)15.1(s)0(u)20(s)-502.7(as)-498.2(to)-493.3(wh)17.3(et)18.4(he)15.1(r)-503.1(t)0(h)18.9(e)-507.6(fi)17.3(rs)18.4(t)-503.9(B)11.6(AL)]TJ -1.0015 -1.0471 TD [(sa)19.6(mp)12.4(le)-442.5(sh)14.4(ou)21.2(ld)-447.8(be)-440.1(pr)13.9(oc)20.8(es)13.9(se)19.6(d)-456(s)0(e)19.6(p)0(a)15.1(r)0(a)19.2(t)0(e)18.4(l)0(y)-447.8(o)0(r)-441.3(w)0(h)11.6(e)0(t)18.4(h)0(e)20.8(r)-457.6(it)-444.5(is)]TJ 0 -1.0528 TD [(ac)14.7(ce)20.4(pt)18.9(ab)15.1(le)-197.8(to)-197.4(po)15.5(ol)-197.4(al)18.4(l)-208(s)0(p)14.4(e)0(c)20.4(i)0(m)15.7(e)0(n)15.1(s)0(.)-195.8(T)0(h)17.1(e)-206(fi)17.3(rs)18.4(t)-208(B)0(A)12.6(L)-204.1(sa)13.9(mp)18.1(le)-197.8(ha)15.1(s)]TJ T* [(a)-359.7(l)0(o)18.9(w)0(e)16.9(r)-360.9(ce)14.7(ll)16.5(ul)18.9(ar)-350.7(yi)18.9(el)18.5(d)-359.3(a)0(n)15.1(d)-359.3(co)15.1(nt)18.9(ai)18.4(ns)-349.8(mo)18.1(re)-356.3(ne)20.8(ut)18.9(ro)13.9(ph)21.2(il)16.5(s)-360.5(a)0(n)15.1(d)]TJ 0 -1.0471 TD [(le)18.4(ss)-573(ly)13.2(mp)18.1(ho)21.2(cy)15.1(te)18.4(s)-582.4(t)0(h)18.9(a)0(n)-576.7(t)0(h)18.9(e)-581.6(fo)13.9(ll)16.5(ow)17.3(in)18.9(g)-581.2(s)0(a)19.6(m)0(p)12.4(l)0(e)18.4(s)-582.4(in)-573(bo)15.5(th)]TJ 0 -1.0527 TD [(he)15.1(al)18.5(th)18.8(y)-256.8(c)0(h)15.1(i)0(l)22.2(d)0(r)13.9(e)0(n)-246.6(a)0(n)15.1(d)-256.8(th)18.9(os)20(e)-257.2(w)0(i)14.9(t)0(h)-248.6(l)0(u)18.9(n)0(g)-251.9(d)0(i)18.9(s)0(e)19.6(a)0(s)13.9(e)0(s)-247.8([)0(1)19.6(5)0(,)-245.8(3)0(3)15.5(,)-256.5(34)15.5(].)]TJ 0 -1.0471 TD [(S)11.4(u)0(b)15.5(s)0(e)19.6(q)0(u)15.5(e)0(n)15.1(t)-373(sa)19.6(mp)18.1(le)12.8(s)-371.8(c)0(a)14.7(n)-370.7(be)-366.1(po)15.5(ol)18.9(ed)-366.1(fo)19.6(r)-372.2(c)0(e)14.7(l)0(l)16.5(u)0(l)18.9(a)0(r)-367.7(a)0(n)20.8(d)-376.3(no)21.2(n-)]TJ 0 -1.0527 TD [(ce)14.7(l)13.9(l)0(u)13.2(l)0(a)18.4(r)-457.6(st)17.7(ud)15.5(ie)18.4(s.)-451.9(Th)17.1(e)-462.1(f)0(i)17.3(r)0(s)18.4(t)-458.4(sa)13.9(mp)18.1(le)-453.9(ma)17.7(y)-461.7(b)0(e)-451.5(o)0(f)-447(i)0(n)13.2(t)0(e)18.4(r)0(e)19.2(s)0(t)-454.7(i)0(n)]TJ 0 -1.0471 TD [(di)18.9(se)13.9(as)19.6(es)-162.5(wi)14.9(th)-157.6(a)-171.9(p)0(r)19.6(i)0(m)15.7(a)0(r)13.5(i)0(l)16.5(y)-165.8(br)13.9(on)21.2(ch)15.1(ia)18.4(l)-173.9(c)0(o)20.8(m)0(p)18.1(o)0(n)15.5(e)0(n)20.8(t)-173.9(su)20(ch)-161.3(as)-162.5(as)13.9(th)18.9(-)]TJ 0 -1.0528 TD [(ma)-124.6(an)15.1(d)-131.6(b)0(r)14(o)0(n)21.2(c)0(h)15.1(i)0(t)16.5(i)0(s)17.7(.)-131.3(In)-128.3(ad)20.8(di)18.9(ti)16.5(on)15.5(,)-131.3(a)0(l)18.4(l)-134(or)-128.3(pa)20.8(rt)-130.7(of)-122.6(th)18.9(e)-137.7(f)0(i)17.3(r)0(s)18.4(t)-134(sa)19.6(mp)12.4(le)-123.8(is)]TJ 0 -1.0471 TD [(of)19.6(te)12.8(n)-131.7(u)0(s)20(e)0(d)-121.4(f)0(o)13.9(r)-133.2(mi)15.7(cr)19.2(ob)15.5(io)18.9(lo)18.9(gi)18.9(ca)14.7(l)-134(c)0(u)20.8(l)0(t)16.5(u)0(r)19.6(e)0(s)13.9(.)-131.3(T)12.2(h)0(e)-127.1(f)0(l)17.3(u)0(i)18.9(d)-131.7(re)13.5(co)20.8(ve)15.1(ry)-122.6(is)]TJ 0 -1.0528 TD [(lo)18.9(we)16.9(r)-207.2(i)0(n)-191.7(n)0(o)15.5(n)0(b)15.5(r)0(o)19.6(n)0(c)15.1(h)0(o)21.2(s)0(c)19.6(o)0(p)15.5(i)0(c)-197.8(l)0(a)18.4(v)0(a)20.8(g)0(e)-195.4(a)0(n)15.1(d)-205.6(co)20.8(mp)12.4(ar)19.2(at)18.4(iv)18.9(e)-206(s)0(t)17.7(u)0(d)15.5(i)0(e)18.4(s)]TJ 0 -1.0471 TD [(be)15.1(tw)20.6(ee)14.7(n)-547.1(f)0(o)19.6(r)0(m)16.5(a)0(l)-539.2(B)11.6(AL)-538.8(an)15.1(d)-547.1(n)0(o)21.2(n)0(b)15.5(r)0(o)19.6(n)0(c)15.1(h)0(o)21.2(s)0(c)13.9(o)0(p)21.2(i)0(c)-539.2(B)11.6(AL)-538.8(ar)13.5(e)]TJ 0 -1.0528 TD [(ne)15.1(ed)20.8(ed)-451.5(to)-447.8(de)20.8(fi)17.3(ne)-451.5(wh)17.3(et)18.4(he)15.1(r)-457.6(b)0(o)21.2(t)0(h)-447.8(t)0(e)18.4(c)0(h)15.1(n)0(i)18.9(q)0(u)15.5(e)0(s)-447(y)0(i)18.9(e)0(l)18.4(d)-461.7(si)17.7(mi)15.7(la)18.4(r)]TJ 0 -1.0471 TD [(re)19.2(su)14.4(lt)16.5(s)-320.6(f)0(o)13.9(r)-321(ce)14.7(ll)16.5(ul)18.9(ar)-316.5(an)20.8(d)-325.1(n)0(o)15.5(n)0(c)20.8(e)0(l)18.4(l)0(u)13.2(l)0(a)18.4(r)-321(BA)12.6(L)-323.6(c)0(o)15.1(n)0(s)20(t)0(i)16.5(t)0(u)18.9(e)0(n)15.1(t)0(s)17.7(.)]TJ /F8 1 Tf 25.1977 66.1583 TD [(Cel)-9.8(lular)-305.7(compo)-8.1(nents)]TJ /F4 1 Tf 1.0015 -1.747 TD [(Th)17.1(e)-262.9(p)0(r)13.9(o)0(c)20.8(e)0(s)13.9(s)0(i)17.7(n)0(g)-251.9(o)0(f)-253.5(B)0(A)12.6(L)-261(fl)17.3(ui)18.9(d)-268.2(i)13.9(n)-268.2(c)0(h)20.8(i)0(l)16.5(d)0(r)19.6(e)0(n)-258(i)0(s)-255.5(n)0(o)21.2(t)-264.9(di)18.9(f)20.4(f)0(e)13.5(r)0(e)19.2(n)0(t)]TJ -1.0015 -1.0755 TD [(to)-351(th)18.9(at)-345.7(us)14.4(ed)-343.4(in)-351(ad)20.8(ul)18.9(ts)-352.2([3)19.6(].)-366.9(The)-371(recovered)-375.9(volume)-369(shoul)-7.4(d)]TJ T* [(be)-696.2(measured)-696.1(and)-696.9(total)-694.2(cell)-697.2(counts)-691.4(p)-6.5(erformed.)-698.1(Cell)]TJ T* [(viabi)-9(lity)-306.1(should)-304.1(be)-297.8(asses)-8.2(sed,)]TJ /F8 1 Tf 11.7112 0 TD (e.g.)Tj /F4 1 Tf 1.7356 0 TD [(using)-303.3(trypa)-8.2(n)-302.4(exclusion.)]TJ -13.4468 -1.0698 TD [(Di)-7.8(ffer)8.8(enti)-8.2(al)-260.4(cytol)-9(ogy)-264.1(is)-261.2(usual)-7.8(ly)-260(perfor)-9.8(med)-261.9(on)-263.3(cell)-264.7(prepar-)]TJ 0 -1.0755 TD 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[(re)19.2(po)15.5(rt)-278.6(wh)17.3(ic)18.4(h)-291(a)0(l)18.5(s)0(o)-281.6(i)0(n)18.9(c)0(l)18.5(u)0(d)15.5(e)0(s)-276.3(a)-291.4(ch)20.8(ap)15.1(te)18.4(r)-286.9(o)0(n)-280.4(s)0(p)20(e)0(c)14.7(i)0(a)18.4(l)-287.7(co)15.1(ns)20(id)18.9(er)13.5(a-)]TJ T* [(ti)16.5(on)21.2(s)-565.3(i)0(n)-550.2(c)0(h)15.1(i)0(l)22.2(d)0(r)14(e)0(n)-553.9([)0(4)19.6(5].)-561.2(Most)-577.6(of)-572.2(the)-574.1(measurem)-10.7(ents)-576.1(of)]TJ T* [(noncel)-7.8(lular)-306.5(BAL)-299.6(flui)-9.4(d)-296.7(compo)-7.4(nents)-303.7(have)-305.5(been)-299.8(perfor)-9.8(med)]TJ T* [(on)-365.7(supernatant)-370(obtai)-9(ned)-361.2(after)-368.7(centr)-8.6(ifuga)-8.2(tion)-360.6(and)-366.9(separa)-8.6(-)]TJ T* [(tion)-263.9(of)-253.5(BAL)-265.4(cells.)-261.3(Studi)-7.8(es)-259.2(of)-259.2(surfactant)-268.4(compo)-7.4(nents)-263.9(need)]TJ 0 -1.0471 TD [(speci)-8.2(al)-379.9(considerat)-10.7(ion)-380.3(as)-378.7(large)-372.8(surfa)-8.6(ctant)-379.3(aggrega)-8.1(tes)-381.8(will)]TJ 0 -1.0527 TD [(be)-593.8(trapped)-596.3(in)-590(the)-591.2(cell)-8.6(ular)-593.6(fraction)-601(if)-591.6(a)-587.3(convent)-8.5(ional)]TJ T* [(centr)-8.6(ifugation)-237.9(speed)-233.4(\(500)]TJ /F5 1 Tf 10.3283 0 TD 0 Tw (6)Tj /F8 1 Tf 1.0015 0 TD (g)Tj /F4 1 Tf 0.7284 0 TD [(or)-230.7(higher\))-233.2(is)-232.7(used.)-232.6(Wheth)-7.8(er)]TJ -12.0583 -1.0527 TD [(fresh)-327.3(or)-327.5(frozen)-332.9(BAL)-328(fluid)-328.8(specimens)-334.7(shoul)-7.4(d)-319.4(b)0(e)-326.3(used)-329(for)]TJ T* [(analys)-9(is)-369.3(has)-373.8(to)-368.1(be)-371.8(deter)-8.6(mined)-373.9(for)-375.4(every)-370.4(param)-9.4(eter)-372(indi)-7.8(-)]TJ T* [(vidual)-9.7(ly)64.4(.)-233.7(Most)-241.9(prote)-8.2(ins)-239.2(appear)-240.7(to)-237.2(be)-235.2(stable)-239(if)-238.8(BAL)-242.7(fluid)-237.8(is)]TJ T* [(stored)-237.8(at)-226.2(-80)]TJ /F5 1 Tf 4.9109 0 TD (8)Tj /F4 1 Tf 0.387 0 TD [(C.)-233.5(Because)-234.8(of)-230.7(the)-232.7(low)-230.5(concent)-8.9(ration)-233.3(of)-230.7(most)]TJ -5.2979 -1.0527 TD [(noncel)-7.8(lular)-243.9(BAL)-248.4(compon)-8.1(ents,)-240.7(many)-245.6(bioas)-7.8(says)-244.8(have)-242.9(to)-242.9(be)]TJ 0 -1.0471 TD [(adjus)-7.8(ted)-209.9(to)-208.8(a)-211.7(more)-213.1(sensi)-9(tive)-213.1(range.)-211.5(It)-216.1(is)-210(advisable)-218.9(to)-208.8(use)-214.4(an)]TJ 0 -1.0527 TD [(assay)-223.2(on)-217.8(unconcen)-7.3(trat)-9.8(ed)-218.2(BAL)-219.9(flui)-9.4(d)-217(a)0(s)-219.4(concent)-8.9(ration)-221.9(steps)]TJ T* [(may)-370(affect)-364.2(the)-369.3(actual)-374.7(quantity)-372.2(o)-6.5(f)-366.6(the)-369.3(compo)-7.4(nent.)-370.4(In)-367.3(the)]TJ T* [(absence)-514.6(of)-509.6(any)-509.2(reli)-9.8(able)-512.7(reference)-514.4(subst)-8.6(ance,)-511.1(meas)-8.2(ure-)]TJ T* [(ments)-397.9(of)-395.8(noncel)-7.8(lular)-397.5(constitu)-9.1(ents)-394(shoul)-7.4(d)-393.4(b)0(e)-394.6(reported)-399.4(in)]TJ T* [(concent)-8.9(rations)-343.3(per)-334.3(millilit)-14.5(re)-333.6(of)-333.2(recover)-9.7(ed)-337.7(BAL)-339.4(fluid.)]TJ /F8 1 Tf 0 -3.1013 TD [(Recomm)-11.7(endations)]TJ /F4 1 Tf 1.0015 -1.747 TD [(1\))-304.7(T)12.2(h)0(e)-303.5(r)0(e)13.5(c)0(o)20.8(v)0(e)15.1(r)0(e)19.2(d)-313.7(B)11.6(A)0(L)-305.5(f)0(l)17.3(u)0(i)18.9(d)-313.7(sh)20(ou)15.5(ld)-299.8(be)-303.5(fi)17.3(lt)16.5(er)13.5(ed)-297.8(th)13.2(ro)19.6(ug)15.5(h)]TJ -1.0015 -1.0528 TD [(on)15.5(e)-496.3(l)0(a)12.8(y)0(e)20.8(r)-503.1(of)-492.5(st)17.7(er)19.2(il)16.5(e)-501.9(g)0(a)20.8(u)0(z)15.1(e)-501.9(af)19.2(te)18.4(r)-503.1(s)0(e)19.6(p)0(a)15.1(r)0(a)19.2(t)0(i)16.5(n)0(g)-490.9(m)0(a)17.7(t)0(e)12.8(r)0(i)17.3(a)0(l)-488(f)0(o)13.9(r)]TJ T* [(mi)15.7(cr)19.2(ob)15.5(io)18.9(lo)18.8(gi)18.9(ca)14.7(l)-526.7(s)0(t)17.7(u)0(d)21.2(i)0(e)12.8(s)0(;)-517.2(2)0(\))-515.3(w)0(h)17.3(i)0(l)16.5(e)-524.7(th)18.9(er)13.5(e)-524.7(i)0(s)-517.3(c)0(u)15.1(r)0(r)18(e)0(n)20.8(t)0(l)16.5(y)-524.3(no)]TJ T* [(co)15.1(ns)20(en)15.1(su)20(s)-275.1(a)0(s)-270.6(t)0(o)-265.7(w)0(h)17.3(e)0(t)12.8(h)0(e)20.8(r)-275.5(th)13.2(e)-274.3(f)0(i)17.3(r)0(s)18.4(t)-282(sa)19.6(mp)18.1(le)-271.8(sh)20(ou)15.5(ld)-265.7(be)-269.4(tr)17.3(ea)20.4(te)12.8(d)]TJ T* [(di)18.9(f)20.4(f)0(e)13.5(r)0(e)19.2(n)0(t)18.9(l)0(y)75.8(,)-228(th)13.2(e)-228.8(f)0(i)17.3(r)0(s)12.8(t)-230.8(sa)19.6(mp)12.4(le)-220.6(is)-227(of)-225.1(mo)18.1(re)-225.5(br)19.6(on)15.5(ch)20.8(ia)18.4(l)-236.5(o)0(r)19.6(i)0(g)18.9(i)0(n)13.2(,)-228(ha)15.1(s)]TJ T* [(a)-336.9(s)0(m)16.9(a)0(l)18.4(l)0(e)18.4(r)-338.1(vo)15.5(lu)18.9(me)-329.4(an)20.8(d)-336.5(c)0(o)15.1(n)0(t)18.9(a)0(i)18.4(n)0(s)-327.1(m)0(o)12.4(r)0(e)-327.9(n)0(e)20.8(u)0(t)18.9(r)0(o)13.9(p)0(h)21.2(i)0(l)16.5(s)-337.7(an)15.1(d)-330.8(l)0(e)12.8(s)0(s)]TJ T* [(ly)18.9(mp)18.1(ho)15.5(cy)15.1(te)18.4(s)-326.3(t)0(h)18.9(a)0(n)-314.9(s)0(u)14.4(b)0(s)20(e)0(q)15.1(u)0(e)20.8(n)0(t)-316.9(s)0(a)13.9(m)0(p)18.1(l)0(e)18.4(s)0(;)-318.1(3)0(\))-316.1(d)0(i)18.9(f)14.7(fe)19.2(re)19.2(nt)13.2(i)13.9(a)0(l)-317.3(c)0(e)14.7(l)0(l)]TJ 0 -1.0471 TD [(co)15.1(un)21.2(ts)-272.6(sh)20(ou)15.5(ld)-265.7(be)-269.4(pe)15.1(rf)18(or)13.9(me)17.7(d)-279.6(o)0(n)-269(a)0(t)-271.8(l)13.9(ea)14.7(st)-272.6(30)21.2(0)-279.6(c)0(e)14.7(l)13.9(ls)-272.6(ei)18.4(th)18.9(er)-271(by)]TJ 0 -1.0527 TD [(cy)15.1(to)18.9(sp)20(in)18.8(s,)-389.3(sm)16.9(ea)14.7(rs)-385.6(or)-390.1(fl)17.3(ow)-392.4(cy)20.8(to)13.2(me)17.7(tr)17.3(y;)-385.2(4\))-390.1(th)13.2(e)-393.8(p)0(r)13.9(e)0(s)19.6(e)0(n)15.1(c)0(e)-389.3(o)0(f)]TJ T* [(ep)15.1(it)22.2(he)15.1(li)16.5(al)-425.4(ce)14.7(ll)16.5(s)-434.4(s)0(h)14.3(o)0(u)21.2(l)0(d)-430.7(b)0(e)-428.7(n)0(o)21.2(t)0(e)12.8(d)-433.3(bu)15.5(t)-435.6(n)0(o)15.5(t)-435.6(in)18.9(cl)18.4(ud)15.5(ed)-428.7(in)-425(th)13.2(e)]TJ T* [(di)18.9(f)20.4(f)0(e)13.5(r)0(e)19.2(n)0(t)18.9(i)0(a)12.8(l)-282(ce)20.4(ll)-273.7(co)15.1(un)21.2(t;)-273.7(5\))-270.6(ma)17.7(te)12.8(ri)17.3(al)-271.8(fo)19.6(r)-281.2(t)0(h)13.2(e)-280(de)20.8(te)12.8(rm)16.5(in)18.9(at)18.4(io)18.9(n)-285.3(o)0(f)]TJ T* [(no)15.5(nc)20.8(el)18.5(lu)13.2(l)13.9(a)0(r)-373.4(c)0(o)20.8(n)0(s)14.4(t)0(i)16.5(t)0(u)18.9(e)0(n)20.8(t)0(s)-369.3(i)0(s)-369.3(o)0(b)15.5(t)0(a)18.4(i)0(n)18.9(e)0(d)-371.8(f)0(r)18(o)0(m)-368.9(t)0(h)13.2(e)-376.7(su)20(pe)15.1(rn)19.6(at)18.4(an)15.1(t)]TJ T* [(af)19.2(te)12.8(r)-247.1(c)0(e)20.4(n)0(t)18.9(r)0(i)17.3(f)0(u)13.9(g)0(a)20.8(t)0(i)16.5(o)0(n)15.5(.)-245.1(Co)16.5(nc)20.8(en)15.1(tr)17.3(at)18.4(io)18.9(n)-251.2(o)0(f)-236.4(B)0(A)18.3(L)-249.6(fl)17.3(ui)18.9(d)-245.5(s)0(h)14.4(o)0(u)21.2(l)0(d)-237.2(b)0(e)]TJ T* [(av)15.1(oi)18.9(de)20.8(d)-649.5(a)0(s)-634.8(i)0(t)-637.9(m)0(a)17.7(y)-649.5(af)30.6(fe)13.5(ct)-636(th)18.9(e)-649.9(a)0(c)20.4(t)0(u)18.9(a)0(l)-641.7(q)0(u)21.2(a)0(n)15.1(t)0(i)16.5(t)0(y)-635.6(o)0(f)-634.8(t)0(h)13.2(e)]TJ T* [(co)15.1(mp)18.1(on)21.2(en)15.1(t;)-330.6(6\))-333.2(re)13.5(po)21.2(rt)17.3(s)-343.4(o)0(f)-333.2(B)0(A)18.3(L)-346.3(re)19.2(su)20(lt)16.5(s)-343.4(s)0(h)20(o)0(u)15.5(l)0(d)-334(i)13.9(nc)15.1(lu)18.8(de)-332(th)13.2(e)]TJ 0 -1.0471 TD [(vo)15.5(lu)18.8(me)-340.8(in)18.9(st)17.7(il)16.5(le)18.4(d,)-342.6(th)18.9(e)-348.3(v)0(o)15.5(l)0(u)18.9(m)0(e)-340.8(r)0(e)13.5(c)0(o)20.8(v)0(e)15.1(r)0(e)19.2(d)0(,)-342.6(t)0(o)18.9(t)0(a)18.4(l)-350.3(ce)14.7(ll)-336.3(co)15.1(un)15.5(ts)17.7(,)]TJ 0 -1.0528 TD [(pe)15.1(rc)19.2(en)15.1(t)13.9(a)0(g)15.1(e)-354(of)-344.6(ce)14.7(ll)-342(ty)13.2(pe)20.8(s)-354.8(a)0(n)15.1(d)-353.6(fo)19.6(r)-355.2(n)0(o)15.5(n)0(c)20.8(e)0(l)18.5(l)0(u)13.2(l)13.9(ar)-350.7(co)20.8(mp)18.1(on)15.5(en)15.1(ts)17.7(,)]TJ T* [(th)18.9(e)-325.5(c)0(o)15.1(n)0(c)20.8(e)0(n)15.1(t)0(r)17.3(a)0(t)18.5(i)0(o)18.9(n)-325.1(pe)15.1(r)-321(m)0(i)15.7(l)0(l)16.5(i)0(l)16.5(i)0(t)16.5(r)0(e)-316.5(o)0(f)-316.1(B)11.6(AL)-316.9(fl)17.3(ui)18.9(d;)-316.9(7\))-316.1("n)18.3(or)14(ma)17.7(l-)]TJ T* [(iz)18.4(at)18.4(io)13.2(n")-254.9(fo)19.6(r)-269.8(E)12.2(LF)-255.2(sh)14.4(ou)21.2(ld)-260(no)21.2(t)-270.6(r)0(o)19.6(u)0(t)18.9(i)0(n)18.9(e)0(l)12.8(y)-262.5(be)-258(pe)20.8(rf)12.4(or)19.6(me)17.7(d)-268.2(d)0(u)21.2(e)-268.6(to)]TJ T* [(a)-325.5(l)0(a)18.4(c)0(k)-314.9(o)0(f)-316.1(a)-325.5(re)19.2(li)16.5(ab)15.1(l)13.9(e)-325.5(me)12(t)13.9(h)0(o)15.5(d)-325.1(fo)19.6(r)-326.7(i)0(t)16.5(s)-320.6(qu)15.5(an)20.8(ti)16.5(fi)17.3(ca)14.7(ti)16.5(on)21.2(.)]TJ /F8 1 Tf 25.1977 66.1583 TD [(Ar)27.3(eas)-339.6(of)-334(futur)24.4(e)-331.2(study)]TJ /F4 1 Tf 1.0015 -1.747 TD [(1\))-395.8(C)11.6(o)0(m)12.4(p)0(a)20.8(r)0(a)13.5(t)0(i)22.2(v)0(e)-394.6(s)0(t)17.7(u)0(d)15.5(i)0(e)18.4(s)-400.3(of)-390.1(di)18.9(f)14.7(f)0(e)19.2(r)0(e)19.2(n)0(t)-396.6(B)11.6(AL)-396.5(pr)19.6(ot)18.9(oc)15.1(ol)18.8(s)-400.3(i)0(n)]TJ -1.0015 -1.0527 TD [(ch)15.1(il)16.5(dr)19.6(en)-189.7(wi)14.9(th)18.9(ou)15.5(t)-196.6(l)0(u)13.2(n)0(g)-189.3(d)0(i)18.9(s)0(e)19.6(a)0(s)13.9(e)-194.6(to)-191.7(de)15.1(fi)17.3(ne)-189.7(wh)17.3(ic)18.4(h)-199.9(o)0(f)-190.9(t)0(h)18.9(e)-200.3(cu)15.1(rr)18(en)20.8(t)]TJ 0 -1.0471 TD [(pr)13.9(ot)18.9(oc)15.1(ol)18.9(s)-576.7(s)0(h)20(o)0(u)15.5(l)0(d)-567.3(b)0(e)-571(u)0(s)20(e)0(d)-571(i)0(n)-567.3(c)0(h)15.1(i)0(l)16.5(d)0(r)19.6(e)0(n)15.1(;)-577.9(2\))-566.5(co)15.1(mp)18.1(ar)13.5(at)18.4(iv)18.9(e)]TJ 0 -1.0528 TD [(st)17.7(ud)15.5(ie)18.4(s)-650.7(b)0(e)15.1(t)0(w)20.6(e)0(e)14.7(n)-649.5(br)19.6(on)15.5(ch)15.1(os)20(co)15.1(pi)18.9(c)-649.9(a)0(n)20.8(d)-655.2(no)21.2(nb)15.5(ro)19.6(nc)15.1(ho)21.2(sc)13.9(op)21.2(ic)]TJ 0 -1.0471 TD [(BA)12.6(L)-511.3(p)0(r)13.9(o)0(c)20.8(e)0(d)15.1(u)0(r)19.6(e)0(s)13.9(;)-509.6(3\))-509.6(co)20.8(mp)12.4(ar)19.2(at)18.4(iv)18.9(e)-519(s)0(t)17.7(u)0(d)21.2(i)0(e)18.4(s)-514.1(be)15.1(tw)14.9(ee)20.4(n)-512.9(c)0(e)14.7(n)0(-)]TJ 0 -1.0527 TD [(tr)17.3(if)17.3(ug)15.5(ed)-411.7(an)15.1(d)-421.9(u)0(n)15.5(c)0(e)20.4(n)0(t)13.2(r)0(i)17.3(f)0(u)19.6(g)0(e)20.8(d)-427.6(sm)16.9(ea)20.4(rs)-414(of)-418.5(B)11.6(A)0(L)-419.3(c)0(y)20.8(t)0(o)18.9(l)0(o)13.2(g)0(y)21.2(;)-424.2(4\))]TJ T* [(as)13.9(se)19.6(ss)18.9(me)12(nt)-709.5(of)-708.8(th)13.2(e)-718.2(i)0(n)18.9(f)0(l)17.3(u)0(e)15.1(n)0(c)20.8(e)-723.9(of)-708.8(su)20(ct)12.8(i)13.9(o)0(n)-712.8(p)0(r)19.6(e)0(s)13.9(s)0(u)20(r)0(e)-714.8(o)0(n)]TJ 0 -1.0471 TD [(no)15.5(nc)15.1(el)18.4(lu)18.9(la)18.4(r)-326.7(B)11.6(AL)-316.9(co)15.1(ns)20(ti)16.5(tu)18.8(en)15.1(ts)-312.4(in)-316.9(ch)15.1(il)16.5(dr)19.6(en)15.1(.)]TJ /F6 1 Tf 3.8127 -2.1055 TD [(Pr)11(ocessi)-10.1(ng)-336.5(of)-333.2(recover)9.7(ed)-331.2(BAL)-340(flui)-8.6(d:)]TJ 2.6973 -1.0471 TD [(microbiological)-339.1(studie)-8.6(s)]TJ /F4 1 Tf -5.5085 -2.0998 TD [(BA)12.6(L)-317.9(i)0(s)-306.7(w)0(e)16.9(l)0(l)-307.9(e)0(s)13.9(t)0(a)18.5(b)0(l)18.9(i)0(s)17.7(h)0(e)15.1(d)-313.8(in)-311.2(th)18.8(e)-314.1(d)0(i)13.2(a)0(g)20.8(n)0(o)15.5(s)0(i)17.7(s)-314.9(of)-310.4(pu)21.2(lm)15.7(on)15.5(ar)19.2(y)]TJ -1.0015 -1.0528 TD [(in)13.2(fe)19.2(ct)18.4(io)18.9(ns)14.4(,)-592.2(e)0(s)13.9(p)0(e)20.8(c)0(i)18.4(a)0(l)12.8(l)0(y)-584.3(i)0(n)-584.4(i)0(m)15.7(m)0(u)18.1(n)0(o)15.5(c)0(o)15.1(m)0(p)18.1(r)0(o)19.6(m)0(i)15.7(s)0(e)19.6(d)-598.3(ch)20.8(il)16.5(dr)13.9(en)]TJ 0 -1.0471 TD [([1)13.9(8,)-450.7(46)15.5(\2615)21.2(4])13.9(.)-455.6(M)0(o)13.6(l)0(e)18.5(c)0(u)15.1(l)0(a)18.4(r)-463.3(bi)18.9(ol)18.9(og)15.5(y)-461.7(a)0(l)18.5(l)0(o)18.8(w)0(s)-456.2(i)0(d)18.8(e)0(n)20.8(t)0(i)16.5(f)0(i)17.3(c)0(a)14.7(t)0(i)16.5(o)0(n)-451.1(o)0(f)]TJ 0 -1.0528 TD [(pa)15.1(th)18.8(og)15.5(en)20.8(s)-326.3(f)0(r)18(o)0(m)-317.7(s)0(m)16.9(a)0(l)18.5(l)-327.5(sa)19.6(mp)18.1(le)18.5(s)-326.3([)0(5)19.6(5)0(,)-325.5(56].)]TJ /F8 1 Tf 0 -2.0998 TD [(Pr)27.3(ocessi)-10.1(ng)-331.6(of)-334(BAL)-339.8(speci)-8.2(mens)]TJ /F4 1 Tf 1.0015 -1.7527 TD [(Mo)13.6(st)-517.3(au)15.1(th)18.8(or)13.9(s)-525.5(s)0(a)14(m)0(p)18.1(l)0(e)-522.2(t)13.9(he)-519.8(fi)11.6(rs)18.4(t)-526.7(a)0(l)18.5(i)0(q)13.2(u)0(o)21.2(t)-532.4(se)19.6(pa)15.1(ra)19.2(te)18.5(ly)-521.8(fo)19.6(r)]TJ -1.0015 -1.0471 TD [(mi)15.7(cr)13.5(ob)21.2(io)18.8(lo)13.2(gi)18.8(ca)20.4(l)-390.1(s)0(t)17.7(u)0(d)15.5(i)13.9(es)-378.7([1)13.9(9,)-376.7(33)15.5(,)-381.6(3)0(4)15.5(,)-387.3(57)21.2(].)-378.3(Th)17.1(e)-388.1(B)0(A)12.6(L)-386.2(fl)17.3(ui)18.8(d)]TJ 0 -1.0527 TD [(\(B)14.9(AL)13.2(F)11.4(\))-525.9(fo)19.6(r)-520.2(m)0(i)15.7(c)0(r)13.5(o)0(b)21.2(i)0(o)13.2(l)13.9(og)15.5(ic)18.4(al)-516.5(st)17.7(ud)21.2(ie)12.8(s)-519.8(s)0(h)14.4(o)0(u)21.2(l)0(d)-516.1(n)0(o)21.2(t)-526.7(be)-514.1(pu)21.2(t)]TJ 0 -1.0471 TD [(th)13.2(ro)19.6(ug)15.5(h)-399.1(a)0(n)15.1(y)-399.1(ki)13.2(nd)-394.2(of)-390.1(mu)12.4(cu)20.8(s)-406(f)0(i)17.3(l)0(t)16.5(e)0(r)-390.5(b)0(e)15.1(c)0(a)20.4(u)0(s)14.3(e)-399.5(of)-395.8(th)18.9(e)-405.2(r)0(i)17.3(s)0(k)-395.4(o)0(f)]TJ 0 -1.0528 TD [(lo)13.2(si)17.7(ng)-599(ce)20.4(ll)16.5(s)-605.2(a)0(n)15.1(d)-609.7(at)18.5(ta)18.4(ch)15.1(ed)-599.4(di)18.9(ag)20.8(no)15.5(st)17.7(ic)-601.8(m)13.2(a)0(t)12.8(e)0(r)19.2(i)0(a)18.5(l)-612(su)20(ch)-599.4(as)]TJ /F8 1 Tf T* [(Pn)17.1(eu)15.1(mo)17.3(cy)20.4(st)12(i)13.9(s)-303.6(ca)20.8(ri)12(ni)18.9(i)]TJ /F4 1 Tf 8.2228 0 TD [(,)-296.3(a)0(n)15.1(d)-296.7(sh)14.4(ou)21.2(ld)-294.1(be)-292.2(pr)19.6(oc)15.1(es)19.6(se)14(d)-296.7(a)0(s)-293.3(q)0(u)15.5(i)0(c)18.4(k)0(l)18.8(y)]TJ -8.2228 -1.0471 TD [(as)-486.8(po)15.5(ss)18.9(ib)18.9(le)-482.3(to)-487.6(av)20.8(oi)18.9(d)-495.8(f)0(u)19.6(r)0(t)17.3(h)0(e)15.1(r)-491.7(co)15.1(nt)18.9(am)17.7(in)18.9(at)12.8(io)18.9(n)-490.2(a)0(n)15.1(d)-490.2(lo)13.2(ss)-481.9(of)]TJ 0 -1.0527 TD [(ag)15.1(en)15.1(t)13.9(s)-320.6(su)14.3(ch)-309.2(as)-310.4(an)20.8(ae)14.7(ro)19.6(bi)18.9(c)-319.8(b)0(a)15.1(c)0(t)18.4(e)0(r)19.2(i)0(a)-311.6([)0(5)13.9(8].)-316.5(For)-333.1(cultivat)-10.7(ion)-329(of)]TJ 0 -1.0471 TD [(ana)-8.8(erobes,)-310.2(BALF)-305.2(must)-311.4(not)-306.3(be)-303.5(e)-6.9(xposed)-307.8(to)-305.5(air)-308.3(prior)-305.3(to)-305.5(in-)]TJ 0 -1.0528 TD [(ocu)-8.4(lation)-438.9(on)-434(reduced)-440.6(culture)-439.3(medi)-9(um;)-434.6(anaerob)-8.1(ic)-436.8(trans-)]TJ 0 -1.0471 TD [(port)-342.8(media)-340.2(containing)-339.9(reduci)-9.4(ng)-337.3(agents)-339(may)-335.9(be)-337.7(used.)]TJ /F8 1 Tf 0 -2.1055 TD [(T)79.7(echniq)-7.8(ues)]TJ /F4 1 Tf 1.0015 -1.747 TD [(T)69.1(a)0(b)15.1(l)0(e)-465.2(2)-473.1(summarize)-13.8(s)-480(techni)-10.1(ques)-482.7(for)-489.2(the)-483.1(detect)-10.6(ion)-482.7(of)]TJ -1.0015 -1.0528 TD [(mic)-8.2(ro-organisms.)-284.4(As)-279.8(point)-8.6(ed)-275.1(o)-6.5(u)0(t)-277.1(i)0(n)-282.7(the)-278.2(previ)-8.2(ous)-282.3(chapte)-8.2(r)31.8(,)]TJ 0 -1.0471 TD [(mic)-8.2(robiologi)-9.9(cal)-449.3(speci)-8.2(mens)-451.7(should)-452(be)-445.8(obtai)-9(ned)-446.6(prior)-453.3(to)]TJ 0 -1.0527 TD [(fil)-8.6(tration.)-362(Centrifugat)-12.9(ion)-357.5(is)-352.2(usual)-7.8(ly)-351(perfor)-9.8(med)-358.7(for)-352.6(detec-)]TJ 0 -1.0471 TD [(tion)-446(of)-435.6(bacter)-9.8(ia,)-437.2(fungi)-7.8(,)-438.6(protozoa,)-443.7(and)-440.9(viruses)-444.6(by)-439.7(direct)]TJ 0 -1.0528 TD [(ligh)-7.8(t)-327.5(mic)-8.2(roscop)-7.8(y)61.8(.)-330.4(Altern)-9.6(atively)55(,)-330.4(smears)-336.9(can)-333.2(be)-332(prepar)-8.6(ed.)]TJ 0 -1.0471 TD [(Ai)-7.8(r-dr)9.2(ied)-244.1(prepar)-8.6(atio)-8.2(ns)-241.7(allow)-246.2(the)-238.4(use)-242.9(of)-242.1(speci)-8.2(al)-243.3(stains)-245.4(\()]TJ /F8 1 Tf 21.3623 0 TD (e.g.)Tj /F4 1 Tf -21.3623 -1.0528 TD [(Gra)-8.2(m,)-550.6(Papanic)-8.1(olaou,)-553.3(Gomori)-9.9(-Grocott,)-553.7(tolu)-7.8(idine)-549.6(blue\))-8.2(.)]TJ 0 -1.0471 TD [(Fo)-6.4(r)-480.4(bacter)-9.8(ial)-485.5(cult)-8.2(ures)-484.3(uncent)-7.8(rifu)-8.6(ged)-486.4(samples)-492.1(are)-482.7(used;)]TJ 0 -1.0528 TD [(cult)-8.2(ures)-416(for)-415.2(fungi,)-417.9(protozoa,)-421(and)-412.4(viruses)-416.1(are)-420.1(performed)]TJ 0 -1.0471 TD [(after)-340.2(centr)-8.6(ifuga)-8.2(tion.)]TJ 1.0015 -1.0527 TD [(No)11.6(nq)21.2(ua)15.1(nt)18.9(it)16.5(at)18.5(ed)-605.1(ba)20.8(ct)18.5(er)13.5(ia)18.4(l)-612(c)0(u)20.8(l)0(t)16.5(u)0(r)13.9(e)0(s)-600.6(o)0(f)-606.3(B)11.6(AL)13.2(F)-608.9(a)0(r)19.2(e)-615.8(no)21.2(t)]TJ -1.0015 -1.0471 TD [(re)13.5(li)16.5(ab)20.8(le)18.4(,)-421.5(a)0(s)-412.8(t)0(h)18.8(e)0(r)19.2(e)-422.3(is)-409.1(a)-422.3(p)0(o)21.2(t)0(e)18.5(n)0(t)13.2(i)0(a)18.4(l)-418.6(fo)13.9(r)-417.8(c)0(o)20.8(n)0(t)13.2(a)0(m)17.7(i)0(n)18.9(a)0(t)18.5(i)0(o)13.2(n)-416.2(of)-412.8(th)18.9(e)]TJ 0 -1.0528 TD [(su)14.4(ct)18.4(io)18.9(n)-478.8(c)0(h)15.1(a)0(n)20.8(n)0(e)15.1(l)-475.5(of)-464.1(fl)17.3(ex)20.8(ib)18.8(le)-470.9(br)13.9(on)21.2(ch)15.1(os)20(co)15.1(pe)20.8(s.)-469(Qu)17.3(an)15.1(ti)16.5(ta)18.5(ti)16.5(ve)]TJ 0 -1.0471 TD [(cu)15.1(lt)16.5(ur)19.6(es)13.9(,)-427.2(b)0(i)18.9(l)0(a)12.8(t)0(e)18.4(r)0(a)19.2(l)-435.6(B)11.6(A)0(L)-425(i)0(n)-425(p)0(a)20.8(t)0(i)16.5(e)0(n)15.1(t)0(s)-420.5(w)0(i)14.9(t)0(h)-419.3(u)0(n)15.5(i)0(l)16.5(a)0(t)18.5(e)0(r)19.2(a)0(l)-425.4(p)0(n)15.5(e)0(u)20.8(-)]TJ 0 -1.0527 TD [(mo)12.4(ni)18.9(a,)-308.8(an)20.8(d)-319.4(p)0(r)19.6(o)0(t)18.9(e)0(c)14.7(t)0(e)18.4(d)-319.4(B)11.6(A)0(L)-311.2(h)0(a)20.8(v)0(e)-309.2(b)0(e)15.1(e)0(n)-309.2(p)0(r)19.6(o)0(p)15.5(o)0(s)20(e)0(d)-309.2(a)0(s)-304.7(m)0(e)12(a)0(n)20.8(s)]TJ 0 -1.0471 TD [(to)-254.3(di)13.2(f)20.4(f)0(e)19.2(r)0(e)13.5(n)0(t)18.9(i)0(a)18.4(t)0(e)-254.7(b)0(e)15.1(t)0(w)20.6(e)0(e)14.7(n)-262.5(ba)15.1(ct)18.5(er)19.2(ia)18.4(l)-264.9(i)0(n)18.8(f)0(e)13.5(c)0(t)18.5(i)0(o)18.9(n)-262.5(an)15.1(d)-262.5(c)0(o)20.8(n)0(t)18.9(a)0(m)12(i)0(n)18.8(a)0(-)]TJ 0 -1.0528 TD 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[(ination)-468.1(of)-464.1(the)-466(chest)-8.2(,)-461.3(and)-463.6(norm)-7.8(al)-459.6(bronchi)-9.7(al)-465.2(mucosa)-470.7(on)]TJ T* [(inspe)-7.8(ction.)-481.7(Patie)-8.6(nts)-478.2(were)-481.7(inves)-7.8(tigated)-485.6(for)-477.8(stridor)42.4(.)-478.4(M)]TJ 6.974 0 0 6.974 275.924 207.383 Tm 0.0001 Tc (ID-)Tj -31.2088 -1.5121 TD (ULLA)Tj /F8 1 Tf 9.963 0 0 9.963 79.937 196.838 Tm 0 Tc [(et)-328.7(al.)]TJ /F4 1 Tf 2.3786 0 TD [([34])-324.9(studied)-330.4(BAL)-322.3(in)-328.3(16)-320.2(child)-9(ren)-323(investig)-9.1(ated)]TJ -4.5524 -1.0584 TD [(for)-489.2(persisten)-10.7(t)-486.8(stridor)25.4(,)-489.8(left)-487.8(lobar)-491.9(emphy)-7.4(sema)-486.2(associ)-10.1(ated)]TJ T* [(with)-393(mild)-392.2(steno)-7.8(sis)-388.3(of)-390.1(the)-392(lingular)-396.5(bronchu)-7.3(s,)-389.3(stenosis)-396.1(of)]TJ T* [(the)-266.8(main)-265.1(left)-265.9(bronchu)-7.3(s)-263.7(o)0(r)-264.9(2)-262.5(mont)-8.6(hs)-258.8(after)-272(the)-261.2(remo)-8.2(val)-266.8(of)-264.9(a)]TJ T* [(foreig)-10.6(n)-245.5(body)59.5(.)-250.8(The)-6.8(se)-247.8(chil)-8.2(dren)-249.7(wer)-8.2(e)-245.9(consi)-7.8(dered)-256.6(free)-251.7(of)-253.5(par-)]TJ 0 -1.0528 TD [(enchym)-8.5(al)-414(lung)-420.8(disea)-8.2(se)-412.8(based)-421.2(on)-416.9(no)-416.9(visua)-7.8(l)-412.9(eviden)-7.8(ce)-417.7(of)]TJ 0 -1.0584 TD [(bronchi)-9.7(al)-180.7(inflamm)-12.7(ation,)-180.1(ster)-8.6(ile)-183.9(BAL)-180.1(cult)-8.2(ure,)-181.1(norm)-7.8(al)-180.7(white)]TJ T* [(blood)-290.7(cell)-293.1(count,)-291.5(normal)-290.9(sedim)-10.9(entation)-292.9(rate)-292.4(and)-287.2(negati)-10.1(ve)]TJ T* [(chest)-213.1(radio)-8.2(graph.)-216.7(T)]TJ 6.974 0 0 6.974 134.079 112.535 Tm 0.0001 Tc (ESSIER)Tj /F8 1 Tf 9.963 0 0 9.963 159.194 112.535 Tm 0 Tc [(et)-214.9(al.)]TJ /F4 1 Tf 2.151 0 TD [([76])-216.8(reported)-217.3(BAL)-214.2(cellular)]TJ 12.9175 29.6193 TD [(compo)-7.4(nents)-235.4(in)-225.8(16)-229.2(children)-235.2(undergoing)-228.2(BAL)-231.3(during)-230.5(endo-)]TJ 0 -1.0584 TD [(scopy)-494.8(to)-493.3(evalua)-8.2(te)-488(stridor)-498.2(or)-492.5(suspec)-7.8(ted)-488.8(inhal)-9(ation)-492.7(of)-492.5(a)]TJ T* [(forei)-9.8(gn)-223.5(body)59.5(.)-216.6(The)-223.1(last)-225.7(five)-229.4(aliquots)-225.4(of)-225.1(aspirated)-231.9(fluid)-226.4(were)]TJ T* [(poo)-8(led)-335.1(and)-332.8(used)-340.4(for)-335.5(cell)-8.6(ular)-337.5(studies.)]TJ 1.0015 -1.0528 TD [(Wh)14.4(er)19.2(ea)14.7(s)-303.6(T)]TJ 6.974 0 0 6.974 362.211 365.499 Tm 0.0001 Tc [(ES)18(SI)20.6(ER)]TJ /F8 1 Tf 9.963 0 0 9.963 387.949 365.499 Tm 0 Tc [(et)-294.5(al)13.2(.)]TJ /F4 1 Tf 2.3104 0 TD [([7)19.6(6])-310.4(reported)-319.8(that)-321.2(BAL)-316.7(cellular)]TJ -10.2032 -1.0584 TD [(ana)-8.8(lysis)-511.7(follow)-9.2(ed)-508.4(a)-513.3(normal)-512.9(dist)-9(ribution)-9.1(,)-506.8(R)]TJ 6.974 0 0 6.974 488.636 354.954 Tm 0.0001 Tc [(A)100.3(T)0(JEN)]TJ /F8 1 Tf 9.963 0 0 9.963 514.148 354.954 Tm 0 Tc [(et)-510.8(al.)]TJ /F4 1 Tf -20.56 -1.0584 TD [([33],)-319.6(R)]TJ 6.974 0 0 6.974 338.003 344.409 Tm 0.0001 Tc [(IEDL)-11.7(ER)]TJ /F8 1 Tf 9.963 0 0 9.963 365.726 344.409 Tm 0 Tc [(et)-311.6(al.)]TJ /F4 1 Tf 2.3559 0 TD [([19])-313.5(and)-315.7(M)]TJ 6.974 0 0 6.974 435.005 344.409 Tm 0.0001 Tc (IDULLA)Tj /F8 1 Tf 9.963 0 0 9.963 463.861 344.409 Tm 0 Tc [(et)-311.6(al.)]TJ /F4 1 Tf 2.3559 0 TD [([34])-313.5(conc)-9.6(lu-)]TJ -17.8683 -1.0584 TD [(ded)-224.6(that)-218.8(it)-222.5(did)-220.9(not.)-215.6(The)-223.1(latter)-224.7(findings)-224.2(are)-220.9(suppor)-7.4(ted)-221.3(by)-217.8(the)]TJ T* [(study)-257.8(o)-6.5(f)-252.7(M)]TJ 6.974 0 0 6.974 352.97 323.32 Tm 0.0001 Tc (ERCHANT)Tj /F8 1 Tf 9.963 0 0 9.963 387.439 323.32 Tm 0 Tc [(et)-254.7(al.)]TJ /F4 1 Tf 2.2421 0 TD [([79])-256.6(in)-254.3(healthy)-261.3(adult)-259.4(volunteers)-10.3(.)]TJ -9.0821 -1.0528 TD [(Ot)14.9(he)15.1(r)-207.2(d)0(i)18.9(s)0(c)14(o)0(r)19.6(d)0(a)20.8(n)0(c)15.1(e)0(s)-202.3(w)12.4(er)13.5(e)-206(o)0(b)15.5(s)0(e)19.6(r)0(v)19.6(e)0(d)-201.1(b)0(e)15.1(t)0(w)20.6(e)0(e)14.7(n)-205.6(BA)12.6(L)-204.1(s)0(t)17.7(u)0(d)15.5(i)0(e)18.4(s)]TJ -1.0015 -1.0584 TD [(in)-220.2(ch)15.1(il)16.5(dr)19.6(en)15.1(,)-228(e)0(s)19.6(p)0(e)20.8(c)0(i)12.8(a)0(l)18.5(l)0(y)-220.2(f)0(o)19.6(r)-230(th)18.9(e)-228.8(n)0(u)21.2(m)0(b)12.4(e)0(r)-219.8(o)0(f)-219.4(c)0(e)20.4(l)0(l)16.5(s)-229.6(pe)20.8(r)-230(m)0(i)15.7(l)0(l)16.5(i)0(l)16.5(i)0(t)16.5(r)0(e)19.2(,)]TJ T* [(pe)15.1(rc)19.2(en)15.1(ta)18.5(ge)-349.1(of)-350.2(ly)18.9(mp)12.4(ho)21.2(cy)15.1(te)18.5(s)-360.5(a)0(n)20.8(d)-359.3(ne)15.1(ut)18.9(ro)19.6(ph)15.5(il)16.5(s)-360.5(\()0(t)17.3(a)0(b)20.8(l)0(e)18.4(s)-360.5(3)-359.3(and)]TJ T* [(4\).)-339.2(The)-342.6(differences)-337(could)-342.3(depend)-341.1(o)-6.5(n)-336.5(children)]TJ /F5 1 Tf 18.352 0 TD 0 Tw (')Tj /F4 1 Tf 0.2219 0 TD [(s)-332(age)-8.8(.)-336.1(The)]TJ -18.574 -1.0584 TD [(pati)-8.2(ents)-234.7(in)-237.2(the)-238.4(paper)-239.5(by)-234.8(R)]TJ 6.974 0 0 6.974 409.946 270.652 Tm 0.0001 Tc (IEDLER)Tj /F8 1 Tf 9.963 0 0 9.963 436.875 270.652 Tm 0 Tc [(et)-237.6(al.)]TJ /F4 1 Tf 2.1965 0 TD [([19])-239.6(are)-238(in)-237.2(the)-232.7(same)]TJ -15.0003 -1.0584 TD [(age)-242.1(range)-239.5(as)-236.4(those)-241.1(in)-237.2(the)-238.4(studies)-240.5(by)-240.5(T)]TJ 6.974 0 0 6.974 456.038 260.107 Tm 0.0001 Tc (ESSIER)Tj /F8 1 Tf 9.963 0 0 9.963 481.436 260.107 Tm 0 Tc [(et)-237.6(al.)]TJ /F4 1 Tf 2.2022 0 TD [([76])-239.6(and)]TJ -19.4787 -1.0584 TD (C)Tj 6.974 0 0 6.974 316.006 249.562 Tm 0.0001 Tc (LEMENT)Tj /F8 1 Tf 9.963 0 0 9.963 346.45 249.562 Tm 0 Tc [(et)-328.7(al.)]TJ /F4 1 Tf 2.3843 0 TD [([77],)-331(but)-323.3(the)-329.4(absol)-7.8(ute)-323.7(numbe)-7.4(r)-326.7(o)0(f)-327.5(subjec)-9(ts)]TJ -6.1117 -1.0584 TD [(studi)-9.8(ed)-332(in)-339.6(a)-331.2(given)-336.7(age)-338.8(are)-334.7(smal)-10.2(l.)]TJ 1.0015 -1.0527 TD [(De)11.2(sp)20(it)16.5(e)-467.8(v)0(a)15.1(r)0(i)17.3(a)0(t)18.5(i)0(o)18.8(n)0(s)-463.7(i)0(n)-459.2(d)0(i)13.2(f)20.4(fe)19.2(re)13.5(nt)18.9(ia)18.4(l)-469.8(c)0(e)14.7(l)0(l)-461.5(c)0(o)20.8(u)0(n)15.5(t)0(s)-460.4(b)0(e)15.1(t)0(w)20.6(e)0(e)14.7(n)]TJ -1.0015 -1.0584 TD [(th)13.2(es)19.6(e)-371.1(s)0(t)17.7(u)0(d)21.2(i)0(e)12.8(s)0(,)-360.8(t)0(h)18.9(e)-371.1(di)18.9(f)20.4(f)0(e)13.5(r)0(e)19.2(n)0(t)18.9(i)0(a)18.4(l)-373(cy)20.8(to)13.2(lo)18.9(gy)-360(of)-361.6(BA)18.3(LF)-363.4(is)-363.6(fa)19.2(ir)17.3(ly)]TJ T* [(si)17.7(mi)15.7(la)12.8(r)-321(t)0(o)-316.9(t)13.9(ha)15.1(t)-321.8(o)0(b)15.5(s)0(e)19.6(r)0(v)13.9(e)0(d)-314.9(i)13.9(n)-325.1(h)0(e)15.1(a)0(l)18.5(t)0(h)18.9(y)-325.1(ad)20.8(ul)18.9(ts)17.7(.)-324.7(I)0(n)19.6(d)0(e)15.1(p)0(e)15.1(n)0(d)21.2(e)0(n)15.1(t)-321.8(of)]TJ T* [(th)13.2(e)-200.3(c)0(h)20.8(i)0(l)16.5(d)]TJ /F5 1 Tf 3.329 0 TD (')Tj /F4 1 Tf 0.2049 0 TD [(s)-201.1(a)0(g)15.1(e)-200.3(ma)17.7(cr)13.5(op)21.2(ha)15.1(ge)20.8(s)-206.8(a)0(r)19.2(e)-200.3(th)18.9(e)-206(p)0(r)19.6(e)0(d)15.1(o)0(m)18.1(i)0(n)18.9(a)0(n)15.1(t)-202.3(ce)20.4(ll)16.5(s)-201.1(i)0(n)-191.7(a)0(l)18.5(l)]TJ -3.5338 -1.0584 TD [(st)17.7(ud)15.5(ie)18.4(s)-309.2(f)0(o)13.9(l)0(l)16.5(o)0(w)17.3(e)0(d)-297.8(b)0(y)-303.1(l)0(y)18.9(m)0(p)18.1(h)0(o)15.5(c)0(y)20.8(t)0(e)18.4(s)0(.)-303.9(T)12.2(he)-303.5(pe)20.8(rc)13.5(en)20.8(ta)12.8(ge)-297.8(of)-299(ne)15.1(u-)]TJ T* [(tr)17.3(op)15.5(hi)18.9(ls)-306.7(ap)15.1(pe)20.8(ar)13.5(s)-309.2(t)0(o)-305.5(b)0(e)-309.2(h)0(i)18.9(g)0(h)15.5(e)0(r)-305.1(i)0(n)-305.5(B)11.6(AL)13.2(F)-313(f)0(r)18(o)0(m)-306.3(c)0(h)15.1(i)0(l)16.5(d)0(r)19.6(e)0(n)]TJ /F9 1 Tf 21.2656 0 TD (<)Tj /F4 1 Tf 0.5463 0 TD (12)Tj -21.8119 -1.0584 TD [(mo)12.4(nt)18.9(hs)-207.6(th)18.9(an)-206.8(fr)18(om)-209.5(ch)20.8(il)16.5(dr)19.6(en)-206.8(ag)15.1(ed)-206.8(13)21.2(\2613)15.5(6)-217(m)0(o)18.1(n)0(t)18.9(h)0(s)-207.6([)0(3)19.6(4].)-219.7(When)]TJ T* [(exp)-8.4(ressed)-305.3(in)-305.5(cells)-306.5(per)-305.9(millilitre)-314(of)-304.7(recovered)-307.6(BAL)-9.4(F)74(,)-302(total)]TJ T* [(cell)-310.2(counts)-304.5(tended)-303.7(to)-305.5(be)-303.5(higher)-304.9(in)-299.8(you)-8(nger)-301(chil)-8.2(dren)-301([33].)]TJ T* [(The)-223.1(maj)-8.2(or)-219.4(dif)10.8(ference)-226.4(b)-6.5(etween)-222.7(chil)-8.2(dren)-221.3(and)-218.9(adult)-9(s)-218.2(i)0(s)-215.6(i)0(n)-220.2(the)]TJ T* [(CD4/)-8.4(CD8)-477.6(ratio)-477.2(which)-477.2(has)-476.2(been)-476.2(found)-477.7(to)-470.5(be)-474.3(low)-8.6(er)-470.2(in)]TJ T* [(chil)-8.2(dren)-335.1(in)-339.6(two)-333(studi)-9.8(es)-333.2([19,)-334.3(3)-6.5(7].)]TJ ET 1 i 58.28 768.302 478.205 -0.51 re f 58.28 742.393 478.205 -0.51 re f 58.28 460.913 478.205 -0.454 re f BT /F3 1 Tf 8.966 0 0 8.966 58.28 772.667 Tm [(Table)-331.9(3.)-336.7(\261)-668.3(Bronchoalveolar)-333.6(lavage)-330.2(\(BAL\))-339.5(differential)-335.3(cell)-332.9(counts)-331.8(from)-334.1(different)-330.7(studies)-335.1(of)-336.7("normal")-330.1(children)]TJ /F4 1 Tf 12.9936 -1.7135 TD (C)Tj 6.276 0 0 6.276 180.794 757.304 Tm 0.0001 Tc (LEMENT)Tj /F8 1 Tf 8.966 0 0 8.966 208.233 757.304 Tm 0 Tc [(et)-336.3(al.)]TJ /F4 1 Tf -1.6566 -1.0496 TD ([78])Tj 6.8287 1.0496 TD (R)Tj 6.276 0 0 6.276 260.561 757.304 Tm 0.0001 Tc [(A)103.9(T)0(JEN)]TJ /F8 1 Tf 8.966 0 0 8.966 281.877 757.304 Tm 0 Tc [(et)-336.3(al.)]TJ /F4 1 Tf -1.3152 -1.0496 TD ([33])Tj 6.4873 1.0496 TD (R)Tj 6.276 0 0 6.276 334.261 757.304 Tm 0.0001 Tc (IEDLER)Tj /F8 1 Tf 8.966 0 0 8.966 359.32 757.304 Tm 0 Tc [(et)-329.9(al.)]TJ /F4 1 Tf -1.5238 -1.0496 TD ([19])Tj 6.6896 1.0496 TD (M)Tj 6.276 0 0 6.276 413.575 757.304 Tm 0.0001 Tc (IDULLA)Tj /F8 1 Tf 8.966 0 0 8.966 439.71 757.304 Tm 0 Tc [(et)-329.9(al.)]TJ /F4 1 Tf -1.6945 -1.0496 TD ([34])Tj 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[(po)15.5(ss)18.9(ib)18.9(le)-550.6(an)15.1(d/)18.9(or)-549.4(ef)30.6(fi)17.3(ci)12.8(en)20.8(t,)-549.8(B)11.6(A)0(L)-550.2(c)0(a)14.7(n)-558.4(pr)19.6(ov)15.5(id)18.8(e)-558.8(s)0(p)20(e)0(c)14.7(i)0(m)15.7(e)0(n)20.8(s)]TJ 0 -1.0471 TD [(fo)13.9(r)-321(m)0(i)15.7(c)0(r)19.2(o)0(b)15.5(i)0(a)18.5(l)-321.8(cu)15.1(lt)16.5(ur)19.6(es)-316.1(as)-316.1(w)12.4(e)0(l)12.8(l)-321.8(as)-316.1(cy)20.8(to)18.9(lo)13.2(gi)18.9(ca)20.4(l)-327.5(e)0(x)20.8(a)0(m)17.7(i)0(n)13.2(a)0(t)18.4(i)0(o)18.8(n)0(.)]TJ 0 -1.0527 TD [(Th)17.1(e)-621.4(d)0(e)15.1(v)0(e)15.1(l)13.9(op)15.5(me)17.7(nt)-612.8(of)-617.7(mo)18.1(le)18.4(cu)15.1(la)18.4(r)-622.6(t)0(e)18.4(c)0(h)15.1(n)0(i)18.9(q)0(u)21.2(e)0(s)-617.7(a)0(l)18.4(l)0(o)18.9(w)0(s)-615.5(f)0(o)19.6(r)]TJ 0 -1.0471 TD [(id)13.2(en)20.8(ti)16.5(fi)17.3(ca)14.7(ti)22.2(on)-331.6(of)-327.5(a)-336.9(n)0(u)21.2(m)0(b)12.4(e)0(r)-327.9(o)0(f)-327.5(m)0(i)15.7(c)0(r)19.2(o)0(-)19.6(o)0(r)25.3(g)0(a)20.8(n)0(i)13.2(s)0(m)16.9(s)0(.)-326.7(H)12.4(ow)11.6(ev)20.8(er)47.7(,)]TJ 0 -1.0527 TD [(th)13.2(e)-433.7(u)0(s)20(e)0(f)19.2(u)0(l)13.2(n)0(e)20.8(s)0(s)-425(o)0(f)-424.2(B)0(A)12.6(L)-431.7(fo)19.6(r)-434.8(t)0(h)18.9(e)-433.7(de)15.1(te)18.4(ct)18.5(io)13.2(n)-433.3(o)0(f)-424.2(i)0(n)18.8(f)0(e)19.2(c)0(t)12.8(i)0(o)18.8(n)-433.3(in)]TJ 0 -1.0471 TD [(th)13.2(e)-484.9(a)0(l)18.4(v)0(e)15.1(o)0(l)18.9(a)0(r)-481.5(c)0(o)15.1(m)0(p)18.1(a)0(r)19.2(t)0(m)15.7(e)0(n)15.1(t)-486.8(ca)14.7(n)-484.5(b)0(e)-479.9(l)0(i)16.5(m)0(i)15.7(t)0(e)18.4(d)-490.2(by)-479.5(co)20.8(nt)13.2(am)17.7(in)18.8(-)]TJ 0 -1.0528 TD [(at)12.8(io)18.9(n.)-376.7(Th)17.1(er)13.5(ef)19.2(or)19.6(e,)-382.8(re)19.2(su)14.4(lt)16.5(s)-388.9(n)0(e)20.8(e)0(d)-383.2(t)0(o)-379.5(b)0(e)-383.2(i)0(n)18.9(t)0(e)18.5(r)0(p)13.9(r)0(e)19.2(t)0(e)18.4(d)-387.7(ba)15.1(se)19.6(d)-393.4(o)0(n)]TJ T* [(qu)15.5(an)15.1(t)13.9(i)0(t)16.5(a)0(t)12.8(i)13.9(ve)-371.8(cu)15.1(l)13.9(t)0(u)13.2(r)0(e)19.2(s)-377.5(wi)14.9(th)-368.1(th)18.9(e)-376.7(u)0(s)14.4(e)-376.8(of)-367.3(a)-382.4(d)0(i)18.9(a)0(g)20.8(n)0(o)15.5(s)0(t)17.7(i)0(c)-368.5(t)0(h)18.8(r)0(e)13.5(s)0(h)20(-)]TJ 0 -1.0471 TD [(ol)13.2(d)-433.3(a)0(n)15.1(d)0(/)18.9(o)0(r)-424.2(i)0(d)13.2(e)0(n)20.8(t)0(i)16.5(f)0(i)17.3(c)0(a)14.7(t)13.9(io)13.2(n)-433.3(o)0(f)-429.9(i)0(n)18.9(t)0(r)17.3(a)0(c)20.4(e)0(l)12.8(l)0(u)18.9(l)0(a)18.5(r)-434.8(ba)15.1(ct)18.4(er)19.2(ia)-431.1(on)-428.3(di)18.9(r)20.4(-)]TJ 0 -1.0528 TD [(ec)14.7(t)-321.8(e)0(x)15.1(a)0(m)17.7(i)0(n)18.9(a)0(t)12.8(i)0(o)18.9(n)-325.1(of)-316.1(t)13.9(h)0(e)-320.6(s)0(a)19.6(m)0(p)18.1(l)0(e)-317.3([)0(6)19.6(0].)]TJ /F8 1 Tf 0 -2.1226 TD [(Th)-6.4(erapeutic)-640(r)32.2(emov)-7.8(al)-629.9(of)-635.6(airways)-635.5(mat)-8.6(erials.)]TJ /F4 1 Tf 19.0975 0 TD [(In)-617.7(l)13.9(i)0(p)13.2(o)0(i)18.8(d)]TJ -19.0975 -1.0528 TD [(pn)15.5(eu)15.1(m)13.2(o)0(n)15.5(i)0(a)18.4(,)-393(BA)12.6(L)-391.8(c)0(a)20.4(n)-393.4(he)15.1(lp)-385.2(re)19.2(mo)18.1(vi)13.2(ng)-382.8(ma)17.7(te)18.4(ri)17.3(al)-385.6(pr)19.6(es)13.9(en)20.8(t)-395.8(i)0(n)]TJ 0 -1.0471 TD [(th)13.2(e)-359.7(a)0(i)12.8(r)0(w)15.7(a)0(y)20.8(s)-366.2([8)19.6(4,)-365.3(85].)-379.8(These)-374.1(pn)-7.2(eumonias)-383.5(result)-376.7(from)-379.3(the)]TJ 0 -1.0015 TD [(presen)-8.2(ce)-389.3(of)-384.4(lipoid)-387.3(mate)-9.4(rial)-385.4(in)-385.2(alveolar)-394.9(structure)-11.1(s,)-383.6(either)]TJ T* [(from)-572.8(external)-571.3(or)-566.5(internal)-573.3(source)-8.2(s.)-560(End)-6.4(ogenous)-570.3(lipoid)]TJ T* [(pne)-8.4(umonia)-260.9(includes)-257.6(va)-7.6(rious)-253.7(d)-6.5(isorders)-259.2(such)-260.7(as)-253.5(choles)-9(terol)]TJ 0 -0.9958 TD [(pne)-8.4(umonia,)-363.7(dissemi)-10.3(nated)-359.8(lipogranul)-11.1(omat)-9(osis)-360.6(and)-355.5(alveo-)]TJ 0 -1.0015 TD [(lar)-382.3(prote)-8.2(inosis.)-382(Althoug)-8.3(h)-376.3(controlle)-10.7(d)-376.3(studies)-382.8(are)-374.6(lacki)-9.4(ng,)]TJ T* [(severa)-8.6(l)-219.4(reports)-223.1(on)-217.8(prote)-8.2(inosis)-222.3(have)-220.1(show)-8.2(n)-217(that)-218.8(whole)-221.1(lung)]TJ T* [(lavage)-514.6(is)-505.9(an)-502.7(effective)-505.8(treatmen)-11.1(t)-503.9(a)0(s)-503.9(i)0(t)-507.1(can)-503.9(remo)-8.2(ve)-502.7(the)]TJ 0 -0.9958 TD [(mat)-8.2(erial)-432.1(from)-436.2(the)-431.9(alveol)-10.1(ar)-430.3(space)-433(an)-7.6(d)-427.6(consequ)-7.3(ently)-435.8(im-)]TJ 0 -1.0015 TD [(proves)-326.4(gas)-322.6(exchan)-11.5(ge)-320.6(and)-321.4(decreas)-9.7(es)-321.8(the)-323.8(risk)-321.2(of)-321.8(infec)-8.6(tion.)]TJ T* [(Appl)-9.4(ication)-593.7(of)-583.6(this)-589.4(technique)-593.3(can)-589.2(be)-582.4(lim)-10.2(ited)-588.7(by)-582(the)]TJ 0 -0.9958 TD [(tole)-8.2(rance)-467.6(of)-458.4(the)-466(child.)-464.6(Howeve)-13.2(r)31.8(,)-461.3(the)-460.3(limit)-10.8(ation)-464.2(can)-464(be)]TJ 0 -1.0015 TD [(ove)-8.4(rcome)-419.1(by)-416.9(performi)-11.1(ng)-416.9(pulmonary)-424.1(lavage)-417.9(of)-418.5(a)-416.6(single)]TJ T* [(lobe)-370(under)-370(endosco)-7.3(py)-371.4(in)-362.4(associ)-10.1(ation)-367.5(wi)-7.8(th)-362.4(ve)-7.6(ntilation)-372(of)]TJ T* [(the)-340.8(other)-338.2(lung)-335.5([86].)]TJ /F8 1 Tf 0 -2.1283 TD [(Di)-7.8(agnosis)-192.2(of)-191.7(no)-7.2(ninfectious)-202(lung)-193.2(diseases.)]TJ /F4 1 Tf 16.4457 0 TD [(Th)17.1(e)-183.3(r)0(o)19.6(l)0(e)-175(o)0(f)-168.1(B)0(A)12.6(L)]TJ -16.4457 -1.0471 TD [(de)15.1(pe)15.1(nd)21.2(s)-354.8(o)0(n)-348.7(t)0(h)13.2(e)-354(pa)15.1(th)18.9(ol)18.9(og)15.5(ic)18.5(al)-345.7(co)15.1(nd)15.5(i)13.9(t)0(i)16.5(o)0(n)15.5(s)-354.8(st)17.7(ud)15.5(ie)18.4(d)-359.3(\()0(t)17.3(a)0(b)20.8(l)0(e)-351.4(5\).)]TJ 0 -1.0527 TD [(BAL)-390.6(can)-384.4(be)-383.2(diagno)-7.4(stic)-385(in)-385.2(alveolar)-389.2(proteinosi)-12.3(s,)-383.6(alveolar)]TJ 0 -1.0471 TD [(hae)-8.8(morrhage,)-491.6(and)-480.7(pulm)-8.6(onary)-483.8(hist)-9(iocytosi)-9.1(s.)-480.3(In)-486.8(alveolar)]TJ 0 -1.0528 TD [(prote)-8.2(inosis,)-364.9(BALF)-362.2(has)-356.7(a)-359.7(milky)-358.8(appe)-9.6(arance)-360.6([85\26187])-8.5(.)-353.2(O)0(n)]TJ 0 -0.9958 TD [(cytoce)-8.2(ntri)-9.4(fugation)-394.1(prepar)-8.6(ations)-392.2(stai)-9.4(ned)-389.7(with)-393(May-Grun)-9.9(-)]TJ 0 -1.0015 TD [(wal)-9(d)-302.4(Giems)-9.2(a,)-303.2(basophi)-9.3(l)-304.7(extra)-8.6(cellular)-312(mat)-8.2(erial)-306.9(mix)-7.8(ed)-303.5(with)]TJ T* [(enlarged)-364.1(foam)-8.2(y)-359.3(a)-6.9(lveolar)-371(macrophages)-374.4(is)-363.6(observ)-7.8(ed.)-366.5(This)]TJ T* [(mat)-8.2(erial)-284.1(and)-275.8(the)-283.9(cytoplasm)-11.1(ic)-277.5(conten)-7.8(t)-276.3(o)-6.5(f)-275.5(the)-278.2(macr)-8.6(ophages)]TJ 0 -0.9958 TD [(displ)-9.8(ay)-275.1(a)-274.3(pink)-272.9(perio)-8.2(dic-acid-Sc)-11.5(hiff)-270.4(staining)-9.1(.)-273.5(New)-278(biolog-)]TJ 0 -1.0015 TD [(ical)-350.1(inves)-7.8(tigatio)-9.6(ns)-344.2(includ)-9.7(e)-342.6(evalua)-8.2(tion)-349.2(of)-344.6(surfa)-8.6(ctant)-345.1(com-)]TJ T* [(pon)-8(ent)-261.1(a)-6.9(ccumulation)-276(in)-265.7(the)-266.8(BAL)-265.4(fluid,)-272.3(such)-266.4(as)-264.9(surfa)-8.6(ctant)]TJ 0 -0.9958 TD [(apo)-8.4(proteins.)-361.6(Idiopathi)-10.3(c)-354(and)-355.5(seconda)-7.7(ry)-355.9(forms)-358.5(of)-355.9(alveolar)]TJ 0 -1.0015 TD [(prote)-8.2(inosis)-341.8(can)-333.2(be)-337.7(differentiated.)]TJ 1.0015 -1.2007 TD [(Th)17.1(e)-445(d)0(i)18.9(a)0(g)15.1(n)0(o)21.2(s)0(i)17.7(s)-445.8(of)-429.9(al)12.8(ve)20.8(ol)18.9(ar)-436(ha)15.1(em)17.7(or)19.6(rh)13.9(ag)20.8(e)-445(i)0(s)-431.9(m)0(a)17.7(d)0(e)-434.4(e)0(a)14.7(s)0(y)]TJ -1.0015 -1.0527 TD [(wh)17.3(en)-252.3(th)13.2(e)-257.2(B)0(A)12.6(L)12.2(F)-261.7(h)0(a)15.1(s)-258(ei)12.8(th)18.9(er)-253.9(a)-257.2(b)0(l)13.2(o)0(o)21.2(d)0(y)-251.9(o)0(r)-253.5(o)0(r)19.6(a)0(n)15.1(g)0(e)-252.3(p)0(i)18.9(n)0(k)-251.9(c)0(o)15.1(l)0(o)18.9(u)0(r)]TJ 0 -1.0471 TD [(an)15.1(d)-467.4(w)12.4(he)15.1(n)-467.4(t)0(h)18.8(e)-462.1(tr)17.3(ia)12.8(d)-461.7(o)0(f)-458.4(h)0(a)15.1(e)0(m)17.7(o)0(p)21.2(t)0(y)13.2(s)0(i)17.7(s)0(,)-451.9(i)0(n)13.2(f)0(i)17.3(l)0(t)22.2(r)0(a)13.5(t)0(e)18.4(s)-462.9(on)-456.8(ch)15.1(es)19.6(t)]TJ 0 -1.0528 TD [(ra)13.5(di)18.9(og)15.5(ra)19.2(ph)-360(an)20.8(d)-370.7(a)0(n)15.1(a)0(e)20.4(m)0(i)15.7(a)-371.1(is)-357.9(pr)13.9(es)19.6(en)15.1(t.)-356.3(Ho)17.3(we)16.9(ve)15.1(r)37.5(,)-364.6(ac)14.7(ti)16.5(ve)-360.4(pu)21.2(l-)]TJ 0 -1.0471 TD [(mo)12.4(na)20.8(ry)-418.5(ha)15.1(em)17.7(or)19.6(rh)13.9(ag)20.8(e)-428(c)0(a)14.7(n)-427.6(oc)20.8(cu)15.1(r)-423.5(w)0(i)14.9(t)0(h)18.9(o)0(u)15.5(t)-429.9(t)13.9(h)0(e)15.1(s)0(e)-418.5(e)0(l)18.4(e)0(m)17.7(e)0(n)15.1(t)0(s)17.7(,)]TJ 0 -1.0528 TD [(an)15.1(d)-205.6(t)0(h)18.9(e)-211.7(gr)19.6(os)20(s)-206.8(a)0(p)15.1(p)0(e)20.8(a)0(r)13.5(a)0(n)20.8(c)0(e)-201.5(o)0(f)-196.6(t)0(h)18.8(e)-206(BA)12.6(L)-204.1(m)0(a)12(y)-205.6(be)-195.4(no)15.5(rm)16.5(al)18.4(.)-205.2(L)0(i)14.7(g)0(h)21.2(t)]TJ 0 -1.0471 TD [(mi)15.7(cr)13.5(os)20(co)15.1(py)-223.5(ex)15.1(am)17.7(in)18.8(at)12.8(io)18.9(n)-234.1(c)0(a)14.7(n)-234.1(sh)14.4(ow)-227.4(th)18.8(e)-240.2(p)0(r)19.6(e)0(s)13.9(e)0(n)20.8(c)0(e)-230(o)0(f)-219.4(f)0(r)18(e)0(e)-230(r)0(e)19.2(d)]TJ 0 -1.0528 TD [(bl)13.2(oo)21.2(d)-404.8(c)0(e)14.7(l)0(l)16.5(s)0(,)-395(r)0(e)13.5(d)-404.8(bl)18.9(oo)15.5(d)-404.8(c)0(e)14.7(l)0(l)22.2(s)-411.7(in)-396.6(al)18.4(ve)15.1(ol)18.9(ar)-396.2(ma)17.7(cr)13.5(op)21.2(ha)15.1(ge)15.1(s,)-395(or)]TJ 0 -1.0471 TD [(ha)15.1(em)17.7(os)14.4(id)18.8(er)19.2(in)-316.9(la)18.4(de)15.1(n)-319.4(a)0(l)12.8(v)0(e)20.8(o)0(l)18.9(a)0(r)-316.5(m)0(a)12(c)0(r)19.2(o)0(p)21.2(h)0(a)15.1(g)0(e)15.1(s)-320.6([8)13.9(8].)]TJ ET 1 i 58.28 768.302 227.168 -0.51 re f 58.28 751.861 227.168 -0.51 re f 58.28 432.567 227.168 -0.51 re f BT /F3 1 Tf 8.966 0 0 8.966 58.28 772.667 Tm [(Table)-331.9(4.)-336.7(\261)-333.2(Lymphocyte)-329(subsets)-337.6(in)-330.2(children)]TJ /F4 1 Tf 8.9027 -1.7135 TD (R)Tj 6.276 0 0 6.276 144.057 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[(wi)14.9(th)-242.9(in)13.2(vo)21.2(lv)18.8(em)12(en)20.8(t)-253.5(o)0(f)-242.1(t)0(h)13.2(e)-251.6(al)18.5(ve)15.1(ol)18.9(ar)-242.5(st)17.7(ru)13.9(ct)18.4(ur)19.6(es)13.9(,)-250.8(B)11.6(AL)-248.6(pr)19.6(ov)15.5(id)18.9(es)]TJ 0 -1.0471 TD [(in)13.2(fo)19.6(rm)16.5(at)18.4(io)18.8(n)-347.9(o)0(n)-337.3(t)0(h)18.9(e)-348.3(pr)13.9(of)19.6(il)16.5(es)-338.9(of)-338.9(ce)20.4(ll)-336.3(po)15.5(pu)15.5(l)13.9(a)0(t)12.8(i)0(o)18.9(n)-347.9(an)20.8(d)-347.9(c)0(o)15.1(n)0(s)20(e)0(-)]TJ 0 -1.0527 TD [(qu)15.5(en)15.1(t)13.9(l)0(y)-601.4(h)0(e)15.1(l)0(p)18.8(s)-605.2(to)-601.4(or)19.6(ie)18.4(nt)13.2(at)18.5(e)-610.1(t)13.9(he)-599.4(in)13.2(ve)20.8(st)17.7(ig)18.9(at)12.8(io)18.9(ns)20(.)-609.3(B)11.6(AL)-601.4(is)]TJ 0 -1.0471 TD [(th)13.2(er)19.2(ef)19.2(or)13.9(e)-365.4(h)0(e)15.1(l)0(p)18.9(f)0(u)19.6(l)-367.3(fo)13.9(r)-366.6(e)0(v)15.1(a)0(l)18.4(u)0(a)20.8(t)0(i)16.5(o)0(n)-360(o)0(f)-355.9(a)0(l)12.8(v)0(e)20.8(o)0(l)18.9(a)0(r)-362(i)0(n)18.8(f)0(l)17.3(a)0(m)17.7(m)0(a)12(t)0(o)18.9(r)0(y)]TJ 0 -1.0528 TD [(pr)13.9(oc)20.8(es)13.9(se)19.6(s.)]TJ /F8 1 Tf 0 -2.2421 TD [(Ar)27.3(eas)-339.6(for)-335.9(further)-337.2(study)]TJ /F4 1 Tf 1.0015 -1.747 TD [(Th)17.1(e)-348.3(g)0(o)15.5(a)0(l)18.5(s)-349.1(fo)19.6(r)-343.8(t)0(h)13.2(e)-342.6(ne)15.1(xt)-339.6(fe)19.2(w)-346.1(y)0(e)15.1(a)0(r)19.2(s)-349.1(ne)20.8(ed)-337.7(to)-339.6(fo)13.9(cu)20.8(s)-349.1(o)0(n)-337.3(t)0(h)18.9(e)]TJ -1.0015 -1.0527 TD [(de)15.1(fi)17.3(ni)18.9(ti)16.5(on)-229.2(of)-230.7(bi)18.9(ol)18.9(og)15.5(ic)18.4(al)-231.9(ma)17.7(rk)19.6(er)13.5(s)-235.3(i)0(n)-231.5(o)0(r)13.9(d)0(e)20.8(r)-241.4(to)18.9(:)-236.5(1)0(\))-230.7(i)0(m)15.7(p)0(r)19.6(o)0(v)15.5(e)-240.2(th)18.9(e)]TJ 0 -1.0471 TD [(ma)12(na)20.8(ge)15.1(me)17.7(nt)-345.3(of)-344.6(lu)18.9(ng)-343(di)18.9(se)14(as)19.6(es)19.6(;)-356(2)0(\))-344.6(p)0(r)19.6(o)0(v)15.5(i)0(d)18.8(e)-354(in)18.9(fo)19.6(rm)16.5(at)12.8(io)18.8(n)-353.6(o)0(n)]TJ 0 -1.0528 TD [(th)13.2(e)-262.9(p)0(a)20.8(t)0(h)18.8(o)0(g)15.5(e)0(n)15.1(e)0(s)19.6(i)0(s)-255.5(o)0(f)-253.5(v)0(a)20.8(r)0(i)11.6(o)0(u)21.2(s)-263.7(ty)18.8(pe)15.1(s)-263.7(o)0(f)-253.5(l)0(u)18.8(n)0(g)-251.9(d)0(i)13.2(s)0(e)19.6(a)0(s)19.6(e)0(s)13.9(;)-259.2(3\))-259.2(he)20.8(lp)]TJ 0 -1.0471 TD [(pr)13.9(ed)20.8(ic)12.8(t)-287.7(t)0(h)18.9(e)-291.4(ou)21.2(tc)18.4(om)12.4(e)-285.7(a)0(s)-282(w)0(e)16.9(l)0(l)-279.4(a)0(s)-282(t)0(o)-277.1(p)0(r)13.9(e)0(d)20.8(i)0(c)18.4(t)-287.7(th)13.2(e)-285.7(d)0(e)15.1(v)0(e)20.8(l)0(o)13.2(p)0(m)18.1(e)0(n)20.8(t)]TJ 0 -1.0527 TD [(of)-435.6(lu)13.2(ng)-434(di)18.9(se)19.6(as)13.9(e)-439.3(i)0(n)-436.4(a)0(t)18.4(-)0(r)18(i)0(s)12(k)-438.9(pa)15.1(ti)16.5(en)20.8(ts)17.7(;)-447(4)0(\))-435.6(i)0(t)-433.1(i)0(s)-437.6(i)0(m)21.4(p)0(o)15.5(r)0(t)17.3(a)0(n)15.1(t)-441.3(to)]TJ 0 -1.0471 TD [(hi)13.2(gh)21.2(li)16.5(gh)15.5(t)-321.8(t)13.9(ha)15.1(t)-321.8(r)0(e)19.2(s)0(u)14.3(l)0(t)16.5(s)-320.6(re)19.2(po)15.5(rt)17.3(ed)-309.2(in)-311.2(ad)15.1(ul)18.8(t)-321.8(p)0(o)15.5(p)0(u)21.2(l)0(a)18.4(t)0(i)16.5(o)0(n)15.5(s)-320.6(ca)14.7(nn)21.2(ot)]TJ 0 -1.0528 TD [(ap)15.1(pl)18.9(y)-273.9(t)0(o)-265.7(p)0(a)20.8(e)0(d)15.1(i)0(a)18.4(t)0(r)17.3(i)0(c)-266.1(p)0(o)15.5(p)0(u)21.2(l)0(a)18.4(t)0(i)16.5(o)0(n)15.5(s)-275.1(w)12.4(i)0(t)16.5(h)0(o)15.5(u)0(t)-265.7(f)0(u)19.6(r)0(t)17.3(h)0(e)15.1(r)-269.8(ev)15.1(al)18.4(ua)15.1(ti)22.2(on)15.5(.)]TJ /F6 1 Tf 1.1324 -2.2421 TD [(Ind)-7.6(ications)-343.3(and)-336.5(resul)9.7(ts)-340.1(in)-333.2(imm)-9.4(unocompromised)]TJ 8.5188 -1.0471 TD (children)Tj /F4 1 Tf -8.6496 -1.7527 TD [(BA)12.6(L)-272.3(h)0(a)15.1(s)-275.1(be)20.8(co)15.1(me)-266.8(an)-263.7(im)15.7(po)21.2(rt)17.3(an)15.1(t)-270.6(t)0(o)13.2(o)0(l)-260(i)0(n)-265.7(t)0(h)13.2(e)-274.3(ev)20.8(al)18.4(ua)15.1(ti)16.5(on)-263.3(of)]TJ -1.0015 -1.0471 TD [(di)13.2(f)20.4(f)0(u)19.6(s)0(e)-310.4(o)0(r)-310.4(l)0(o)18.9(c)0(a)20.4(l)0(i)16.5(z)0(e)14.7(d)-313.7(pu)15.5(lm)15.7(on)21.2(ar)13.5(y)-313.8(i)0(n)13.2(f)0(i)17.3(l)0(t)22.2(r)0(a)13.5(t)0(e)18.5(s)-314.9(in)-311.2(im)15.7(mu)18.1(no)15.5(co)20.8(m-)]TJ 0 -1.0527 TD [(pr)13.9(om)18.1(is)17.7(ed)-314.9(ch)15.1(il)16.5(dr)19.6(en)-314.9(\(I)18(C\))14.9(.)]TJ /F8 1 Tf 0 -2.2421 TD [(Inter)-9.4(pr)31.4(etat)-8.2(ion)-334.7(of)-334(micr)23.2(obiologic)-8.7(al)-334(r)26.5(esults)]TJ /F4 1 Tf 1.0015 -1.747 TD [(Mi)11.2(cr)19.2(ob)21.2(io)13.2(lo)18.9(gi)18.9(ca)14.7(l)-418.6(s)0(t)17.7(u)0(d)21.2(i)0(e)18.4(s)-423.1(mu)18.1(st)-409.1(be)-411.7(in)18.9(te)18.4(rp)19.6(re)13.5(te)18.4(d)-416.2(w)0(i)14.9(t)0(h)-407.9(c)0(a)14.7(r)0(e)]TJ -1.0015 -1.0527 TD [([7)13.9(0].)-276.6(The)-285.7(identificati)-14.9(on)-280.4(o)-6.5(f)-281.2(primary)-293.3(pathog)-7.4(ens)-282.7(not)-289.2(usually)]TJ 0 -1.0471 TD [(found)-443.6(in)-436.4(the)-437.6(lung)-437.9(in)-436.4(BAL)-441.8(effluent)-430.3(is)-437.6(diagno)-7.4(stic.)-442.3(Such)]TJ 0 -1.0527 TD [(pathog)-7.4(ens)-379.5(incl)-8.2(ude)]TJ /F8 1 Tf 7.7164 0 TD [(P)120.3(.)-376(carinii)]TJ /F4 1 Tf 3.7501 0 TD (,)Tj /F8 1 Tf 0.626 0 TD [(Mycoba)-7.7(cterium)-385.9(tuber)26.3(culo-)]TJ -12.0924 -1.0471 TD (sis)Tj /F4 1 Tf 1.0471 0 TD (,)Tj /F8 1 Tf 0.9276 0 TD [(Legione)-7.7(lla)-678.5(pneum)-8.2(ophila)]TJ /F4 1 Tf 10.1349 0 TD [(,)-677.6(Nocardia,)-687.1(Histop)-8.8(lasma,)]TJ -12.1095 -1.0528 TD [(Bla)-9.8(stomyces,)-277(Mycoplasm)-10.7(a,)-269(Influenza)-275.4(virus)-9(,)-262.1(and)-270.2(Res)-8.6(pira-)]TJ T* [(tory)-513.5(Syncyti)-9.7(al)-505.1(V)52.2(irus)-8.2(.)-506.8(Converse)-8.7(ly)58.7(,)-506.8(the)-505.8(ident)-9(ification)-513.9(of)]TJ 0 -1.0471 TD [(Her)-8.2(pes)-277(simplex)-282.3(virus)-276.4(\(HSV\),)-281.9(CMV)118(,)-267.8(Asp)-7.4(ergillus,)-272.9(atypical)]TJ 0 -1.0527 TD [(mycob)-7.4(acter)-9(ia,)-488.4(bacter)-9.8(ia,)-488.4(and)-492.1(Candida)-496.9(in)-487.6(BAL)-493.1(from)-493.1(IC)]TJ 0 -1.0471 TD [(doe)-8.4(s)-434.4(not)-437.1(nec)-8.8(essarily)-442.4(estab)-8.2(lish)-441.5(a)-433.7(d)-6.5(iagnosis)-440.4(of)-441.3(infec)-8.6(tion,)]TJ 0 -1.0528 TD [(since)-491.9(these)-491.9(organisms)-483.4(may)-489.5(be)-485.6(present)-489.3(as)-486.8(airway)-491.3(con-)]TJ 0 -1.0471 TD [(tam)-8.2(inants)-341(or)-333.2(comm)-9.7(ensals.)]TJ /F8 1 Tf 0 -2.2421 TD [(BAL)-334.1(in)-334(huma)-7(n)-330.8(immuno)-9.1(deficiency)-341.2(virus)-333.3(positive)-339.6(chil)-8.2(dr)31.4(en)]TJ /F4 1 Tf 1.0015 -1.7527 TD [(As)-336.7(su)20(rv)13.9(i)13.9(v)0(a)15.1(l)-338.9(ti)16.5(me)-335.1(ha)20.8(s)-343.4(i)0(n)18.9(c)0(r)19.2(e)0(a)14.7(s)0(e)19.6(d)-342.2(in)-334(pa)20.8(ti)16.5(en)20.8(ts)-335.2(wi)14.9(th)-328.3(hu)15.5(ma)17.7(n)]TJ -1.0015 -1.0471 TD [(im)15.7(mu)12.4(no)21.2(de)15.1(fi)17.3(ci)18.5(en)20.8(cy)-525.5(vi)18.8(ru)13.9(s)-531.2(\()0(H)15.7(I)0(V)21.4(\))0(,)-526.3(t)0(h)18.8(e)-530.4(in)18.9(ci)12.8(de)20.8(nc)15.1(e)-530.4(o)0(f)-521(a)0(c)14.7(u)0(t)18.8(e)]TJ 0 -1.0528 TD [(pn)15.5(eu)15.1(m)13.2(o)0(n)15.5(i)0(a)-556.3(a)0(n)15.1(d)-564.1(re)13.5(cu)20.8(rr)18(en)15.1(t)-566.5(b)0(r)19.6(o)0(n)15.5(c)0(h)15.1(o)0(p)21.2(n)0(e)15.1(u)0(m)18.1(o)0(n)21.2(i)0(a)-562(h)0(a)20.8(s)-571(ri)17.3(se)19.6(n)]TJ 0 -1.0471 TD [([9)13.9(4].)-327.9(In)-333.2(severa)-8.6(l)-338.9(prospe)-7.8(ctive)-339.4(studies)-342.9(of)-333.2(BAL)-345.1(in)-334(HIV)79.3(-pos-)]TJ 0 -1.0528 TD [(itive)-415.4(p)-6.5(atients)-412.8(wi)-7.8(th)-407.9(acute)-417.1(inte)-8.2(rstitial)-419.5(pneumo)-8.1(nitis)-410.5(\(IP\))-416.8(or)]TJ 0 -1.0471 TD [(low)-8.6(er)-458.8(respi)-9.4(ratory)-465.8(tract)-466.2(disease,)-466.9(one)-457.9(or)-464.1(more)-463.4(infec)-8.6(tious)]TJ ET 1 i 58.28 591.364 227.168 -0.51 re f 58.28 112.649 227.168 -0.51 re f BT /F3 1 Tf 8.966 0 0 8.966 58.28 605.197 Tm [(Table)-331.9(5.)-336.7(\261)-333.2(Bronchoalveolar)-649.8(lavage)-652.7(\(BAL\))-649.3(in)-652.6(non-infec-)]TJ 0 -1.0559 TD [(tious)-331.2(lung)-339(diseases)-329.2(in)-336.5(immunocompetent)-334.2(children)]TJ /F6 1 Tf 0 -1.7135 TD [(BAL:)-334.1(essential)-336.8(for)-334.3(diagnosis)]TJ /F4 1 Tf 0 -1.0559 TD [(Alveolar)-339.6(proteinosis)]TJ 0.3351 -1.0496 TD [(Primary)62.2(,)-331.7(idiopathic)]TJ 0 -1.0559 TD (Secondary)Tj T* [(haematological)-338.2(malignancies)]TJ T* [(exposure)-332.7(to)-336.5(inhaled)-329.5(chemicals)-337.4(and)-333.4(minerals)]TJ T* [(lipid)-333.4(pneumonia)]TJ -0.3351 -1.0559 TD [(Alveolar)-339.6(haemorrhage)]TJ 0.3351 -1.0559 TD [(associated)-336.3(with)-334.8(vascularitis)-334.9(and)-333.4(collagen)-335.1(vascular)]TJ 0.3288 -1.0559 TD (diseases)Tj -0.3288 -1.0559 TD [(associated)-336.3(with)-334.8(glomerulonephitis)]TJ T* [(associated)-336.3(with)-334.8(drugs)]TJ T* [(secondary)-332(to)-336.5(cardiac)-334(diseases,)-339.2(pulmonary)-329.2(vascular)]TJ 0.3288 -1.0559 TD [(lesions)-338.5(or)-335.1(malformations)]TJ -0.3288 -1.0559 TD [(associated)-336.3(with)-334.8(haematological)-338.2(malignancies)-338.3(or)]TJ 0.3288 -1.0559 TD (coagulopathy)Tj -0.3288 -1.0559 TD (idiopathic)Tj -0.3351 -1.0559 TD [(Pulmonary)-334.1(histiocytosis)]TJ 0.3351 -1.0559 TD [(class)-330.6(I:)-337.5(Langerhans)-335(cell)-337.4(histiocyosis)]TJ T* 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[(TB)12.4(B)11.6(;)-560.8(4\))-555.1(ch)15.1(ro)19.6(ni)18.9(c)-564.5(r)0(e)19.2(c)0(u)15.1(r)0(r)18(e)0(n)20.8(t)-560.8(br)13.9(on)21.2(ch)15.1(op)21.2(ne)15.1(um)18.1(on)15.5(ia)-550.6(in)-555.9(H)12.4(I)0(V)]TJ 0 -1.0528 TD [(in)13.2(fe)19.2(ct)18.4(ed)-161.3(ch)15.1(il)16.5(dr)19.6(en)-161.3(if)-164.8(or)25.3(ga)20.8(ni)18.9(sm)11.2(s)-167(a)0(r)13.5(e)-171.9(no)21.2(t)-173.9(f)0(o)19.6(u)0(n)15.5(d)-171.5(by)-160.9(le)18.4(ss)-163.2(in)18.9(va)15.1(si)17.7(ve)]TJ 0 -1.0471 TD [(te)12.8(ch)20.8(ni)18.9(qu)15.5(es)19.6(;)-316.1(5)0(\))-310.4(i)0(n)-305.5(a)0(s)13.9(s)0(o)20(c)0(i)18.4(a)0(t)12.8(i)0(o)18.9(n)-313.8(wi)14.9(th)-305.5(TB)12.4(B)-318.5(i)13.9(n)-319.4(l)0(u)18.9(n)0(g)-308.8(t)0(r)17.3(a)0(n)15.1(s)0(p)20(l)0(a)18.4(n)0(t)]TJ 0 -1.0527 TD [(re)13.5(ci)18.4(pi)18.9(en)15.1(ts)-124.6(as)-128.3(pa)15.1(rt)-125(of)-128.3(a)-137.7(r)0(o)19.6(u)0(t)13.2(i)0(n)18.9(e)-137.7(su)20(rv)19.6(ei)12.8(ll)22.2(an)15.1(ce)-127.5(pr)14(og)21.2(ra)19.2(mm)14.9(e)-137.7(a)0(n)15.1(d)0(/)18.9(o)0(r)]TJ T* [(fo)13.9(r)-321(d)0(i)13.2(a)0(g)20.8(n)0(o)15.5(s)0(i)17.7(s)-320.6(of)-316.1(su)14.4(sp)20(ec)14.7(te)18.4(d)-325.1(l)0(u)18.9(n)0(g)-314.5(p)0(a)20.8(t)0(h)13.2(o)0(l)18.9(o)0(g)21.2(y)67.5(.)]TJ /F8 1 Tf 0 -2.0998 TD [(Ar)27.3(eas)-339.6(for)-335.9(further)-337.2(study)]TJ /F4 1 Tf 1.0015 -1.7527 TD [(Al)14.9(th)18.9(ou)15.5(gh)-291.8(BA)18.3(L)-306.5(i)0(s)-289.6(w)0(i)14.9(d)0(e)20.8(l)0(y)-294.1(a)0(c)14.7(c)0(e)20.4(p)0(t)18.9(e)0(d)-292.2(a)0(s)-293.3(a)0(n)-292.1(i)0(m)15.7(p)0(o)15.5(r)0(t)17.3(a)0(n)20.8(t)-304.7(to)18.8(ol)]TJ -1.0015 -1.0471 TD [(in)-288.4(th)18.8(e)-291.4(m)0(a)12(n)0(a)20.8(g)0(e)15.1(m)0(e)17.7(n)0(t)-282.7(o)0(f)-287.7(r)0(e)19.2(s)0(p)20(i)0(r)17.3(a)0(t)12.8(o)0(r)19.6(y)-291(di)13.2(se)19.6(as)19.6(e)-297.1(i)0(n)-282.7(i)0(m)15.7(m)0(u)18.1(n)0(o)15.5(c)0(o)20.8(m)0(-)]TJ 0 -1.0528 TD [(pr)13.9(om)18.1(is)17.7(ed)-309.2(ch)20.8(il)16.5(dr)13.9(en)20.8(,)-319.1(f)0(u)19.6(r)0(t)17.3(h)0(e)15.1(r)-315.3(st)17.7(ud)21.2(y)-319.4(o)0(f)-304.7(B)0(A)12.6(L)-317.9(in)-305.5(th)18.8(is)-306.7(se)14(tt)16.5(in)18.9(g)-313.8(i)0(s)]TJ 0 -1.0471 TD [(re)13.5(qu)21.2(ir)17.3(ed)15.1(.)-381.6(S)11.4(ug)15.5(ge)15.1(st)17.7(ed)-371.8(ar)19.2(ea)14.7(s)-383.2(f)0(o)19.6(r)-383.6(re)19.2(se)13.9(ar)19.2(ch)-371.8(in)13.2(cl)18.5(ud)21.2(e)-382.4(s)0(t)17.7(u)0(d)15.5(i)0(e)18.4(s)-383.2(to)]TJ 0 -1.0528 TD [(de)15.1(te)18.4(rm)16.5(in)18.9(e)-262.9(t)0(h)18.9(e)-262.9(fo)13.9(ll)22.2(ow)11.6(i)13.9(n)0(g)15.5(:)-259.2(1\))-253.5(th)13.2(e)-257.2(m)0(i)15.7(c)0(r)13.5(o)0(b)21.2(i)0(o)18.9(l)0(o)13.2(g)0(i)18.9(c)0(a)20.4(l)-264.9(di)18.9(ag)15.1(no)21.2(st)17.7(ic)]TJ 0 -1.0471 TD [(yi)13.2(el)18.4(d,)-297.1(se)19.6(ns)20(it)16.5(iv)13.2(i)13.9(t)0(y)-299.8(a)0(n)15.1(d)-302.4(sp)14.4(ec)20.4(if)17.3(ic)18.4(it)16.5(y)-308.1(o)0(f)-299(B)11.6(AL)13.2(;)-304.7(2)0(\))-299(t)0(h)18.8(e)-308.5(i)13.9(n)0(c)15.1(i)0(d)18.9(e)0(n)15.1(c)0(e)]TJ 0 -1.0528 TD [(of)-355.9(no)15.5(ni)18.9(nf)19.6(ec)14.7(ti)16.5(ou)21.2(s)-366.2(p)0(u)15.5(l)0(m)15.7(o)0(n)21.2(a)0(r)19.2(y)-365(in)18.8(fi)11.6(l)13.9(t)0(r)17.3(a)0(t)12.8(e)0(s)19.6(;)-367.3(3\))-350.2(th)13.2(e)-359.7(n)0(o)15.5(n)0(c)15.1(e)0(l)18.5(l)0(u)18.8(l)0(a)18.4(r)]TJ 0 -1.0471 TD [(co)15.1(mp)18.1(on)15.5(en)20.8(t)-247.8(i)0(n)-242.9(n)0(o)21.2(n)0(i)18.9(n)0(f)13.9(e)0(c)20.4(t)0(i)16.5(o)0(u)15.5(s)-246.7(in)18.8(vo)15.5(lv)18.8(em)17.7(en)15.1(t;)-239.6(4\))-236.4(a)-251.6(c)0(o)20.8(m)0(p)18.1(a)0(r)13.5(i)0(s)17.7(o)0(n)]TJ 0 -1.0528 TD [(of)-344.6(qu)15.5(an)20.8(ti)16.5(ta)18.4(ti)16.5(ve)-343.4(an)15.1(d)-347.9(s)0(e)14(m)0(i)15.7(-)0(q)19.6(u)0(a)15.1(n)0(t)18.9(i)0(t)16.5(a)0(t)18.4(i)0(v)18.9(e)-354(ba)20.8(ct)18.5(er)13.5(ia)18.4(l)-350.3(c)0(u)15.1(l)0(t)16.5(u)0(r)19.6(e)0(s)-344.6(o)0(f)]TJ 0 -1.0471 TD [(BA)12.6(L)-340.6(w)0(i)14.9(t)0(h)-334(p)0(r)19.6(o)0(t)13.2(e)0(c)20.4(t)0(e)18.4(d)-342.2(br)13.9(us)20(h)-342.2(s)0(a)13.9(m)0(p)18.1(l)0(e)18.4(s)-343.4(to)-334(de)15.1(te)18.4(rm)16.5(in)18.8(e)-342.6(t)0(h)18.8(e)-348.3(se)19.6(n-)]TJ 0 -1.0528 TD [(si)17.7(ti)16.5(vi)13.2(t)13.9(y)-262.5(an)15.1(d)-256.8(s)0(p)14.4(e)0(c)20.4(i)0(f)17.3(i)0(c)18.4(i)0(t)16.5(y)-262.5(of)-247.8(ea)14.7(ch)-252.3(te)18.4(ch)15.1(ni)18.9(qu)21.2(e;)-254.7(5\))-253.5(t)13.9(h)0(e)-252.3(s)0(e)14(n)0(s)20(i)0(t)16.5(i)0(v)18.9(i)0(t)16.5(y)]TJ 0 -1.0471 TD [(an)15.1(d)-251.2(s)0(p)14.4(e)0(c)20.4(i)0(f)17.3(i)0(c)12.8(i)0(t)16.5(y)-251.2(of)-247.8(di)18.9(ag)20.8(no)15.5(st)17.7(ic)-243.3(to)13.2(ol)18.9(s)-252.3(f)0(o)13.9(r)-252.7(CM)14.6(V)137.6(,)]TJ /F8 1 Tf 17.4017 0 TD [(Pn)17.1(eu)20.8(mo)11.6(cy)20.4(st)17.7(ic)]TJ -17.4017 -1.0528 TD [(ca)15.1(ri)17.7(ni)18.9(i)]TJ /F4 1 Tf 2.8453 0 TD [(pn)15.5(eu)15.1(m)13.2(o)0(n)15.5(i)0(a)-254.7(\()0(P)14.7(C)0(P)17.3(\))0(,)-258.8(a)0(n)20.8(d)-268.2(As)16.1(pe)20.8(r)14.7(g)0(i)18.9(l)0(l)16.5(u)0(s)20(;)-264.9(6\))-259.2(th)18.8(e)-268.6(e)0(f)30.6(f)0(e)19.2(c)0(t)-260.4(o)0(f)]TJ -2.8453 -1.0471 TD 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1.0015 -1.7527 TD [(Pr)14.7(oc)20.8(es)13.9(si)17.7(ng)-246.2(of)-247.8(BA)18.3(LF)-249.6(is)-249.8(di)18.9(sc)19.6(us)14.4(se)19.6(d)-256.8(i)0(n)-248.6(d)0(e)15.1(t)13.9(ai)12.8(l)-253.5(e)0(l)12.8(s)0(e)19.6(w)0(h)17.3(e)0(r)19.2(e)0(.)-251.9(I)0(n)]TJ -1.0015 -1.0471 TD [(ne)15.1(on)21.2(at)18.5(ol)13.2(og)21.2(y)73.2(,)-552.4(on)21.2(e)-553.2(o)0(r)-543.7(t)0(w)20.6(o)-552.8(al)18.4(iq)18.9(uo)15.5(ts)-540(of)-532.3(fl)17.3(ui)18.9(d)-552.8(a)0(r)19.2(e)-553.2(us)20(ua)15.1(ll)16.5(y)]TJ 0 -1.0528 TD [(in)18.9(st)17.7(il)16.5(le)18.4(d)-302.4(a)0(n)20.8(d)-302.4(th)18.9(e)-302.8(r)0(e)19.2(c)0(o)15.1(v)0(e)20.8(r)0(e)13.5(d)-296.7(fl)17.3(ui)13.2(d)-296.7(p)0(o)15.5(o)0(l)18.9(e)0(d)-292.2(m)0(a)17.7(i)0(n)18.9(l)0(y)-294.1(d)0(u)21.2(e)-302.8(to)-288.4(th)13.2(e)]TJ 0 -1.0471 TD [(sm)16.9(al)18.4(l)-361.7(v)0(o)15.5(l)0(u)18.9(m)0(e)17.7(s)-360.5(th)13.2(at)-351.4(ar)19.2(e)-359.7(r)0(e)13.5(t)0(u)18.9(r)0(n)19.6(e)0(d)15.1(.)-358.9(So)16.3(me)-352.2(wo)17.3(rk)13.9(er)19.2(s)-360.5(f)0(i)17.3(l)0(t)16.5(e)0(r)-350.7(t)0(h)13.2(e)]TJ 0 -1.0528 TD [(fl)17.3(ui)18.9(d)-222.7(b)0(u)15.5(t)-219.4(in)-214.5(th)18.9(e)-223.1(a)0(u)15.1(t)0(h)18.9(o)0(r)19.6(s)]TJ /F5 1 Tf 8.7691 0 TD (')Tj /F4 1 Tf 0.4439 0 TD [(ex)20.8(pe)15.1(ri)17.3(en)20.8(ce)-218.6(th)18.9(is)-210(re)13.5(su)20(lt)16.5(s)-223.9(i)0(n)-214.5(l)13.9(ow)11.6(er)-214.1(ce)20.4(ll)]TJ -9.213 -1.0471 TD [(co)15.1(un)21.2(ts)-289.6(th)18.9(an)-286.5(in)-288.4(un)15.5(fi)17.3(lt)16.5(er)19.2(ed)-286.5(fl)17.3(ui)13.2(d)-296.7([)0(1)53.8(1)0(4)15.5(])0(.)-310(I)0(n)-304.7(addit)-9(ion,)-306.6(filteri)-12.8(ng)]TJ 0 -1.0528 TD [(is)-249.8(like)-8.2(ly)-248.6(to)-248.6(resul)-9.4(t)-247.8(i)0(n)-248.6(loss)-252.5(of)-253.5(adheren)-8.2(t)-247.8(cell)-8.6(s)-246.6(such)-255(as)-247.8(alveol)-10.1(ar)]TJ 0 -1.0471 TD [(macr)-8.6(ophages)-371.5(and)-366.9(the)-369.3(g)-6.5(auge)-368.1(is)-369.3(likely)-370.6(to)-368.1(soak)-374.5(up)-365.7(BALF)64.6(,)]TJ 0 -1.0528 TD [(thus)-382.2(it)-381.9(may)-387.1(affect)-375.6(the)-380.7(concent)-8.9(ration)-386.9(of)-384.4(the)-380.7(const)-7.8(ituents.)]TJ 0 -1.0471 TD [(The)-558.8(BALF)-555.6(shoul)-7.4(d)-552.8(b)0(e)-553.9(placed)-560.2(on)-553.5(ice)-557.5(after)-562.2(collecti)-11.1(on,)]TJ 0 -1.0528 TD [(proces)-8.2(sed)-237.2(within)-237.6(30)-234.8(min)-235.4(of)-230.7(co)-7.6(llection)-238.4(and)-236(the)-232.7(supern)-7.8(atant)]TJ 0 -1.0471 TD [(stored)-249.2(at)-243.3(-70)]TJ /F5 1 Tf 4.9394 0 TD (8)Tj /F4 1 Tf 0.387 0 TD [(C.)-244.9(Analysi)-9.9(s)-241(o)0(f)-247.8(the)-244.1(supern)-7.8(atant)-248.4(and)-241.7(cell)-8.6(s)-241(will)]TJ -5.3264 -1.0528 TD [(depend)-437.9(on)-439.7(the)-431.9(aim)-8.2(s)-434.4(o)0(f)-435.6(the)-437.6(study)-434.2(b)-6.5(u)0(t)-430.7(the)-437.6(met)-8.2(hodolo)-7(gy)]TJ 0 -1.0471 TD [(shoul)-7.4(d)-444.6(b)0(e)-451.5(optimi)-9.2(zed)-447(for)-449.3(the)-454.6(particula)-11.1(r)-446.2(quest)-7.8(ions)-450.5(being)]TJ 0 -1.0527 TD [(asked.)-376.1(In)-378.7(particula)-11.1(r)31.8(,)-376(the)-375(supern)-7.8(atant)-373.6(shoul)-7.4(d)-370.7(b)0(e)-377.5(analys)-9(ed)]TJ T* [(withi)-9.2(n)-222.7(6)-228.4(mont)-8.6(hs)-224.7(as)-230.7(the)-227(effect)-227.6(of)-225.1(prolo)-7.8(nged)-231.1(storage)-233.2(even)-231.5(at)]TJ 0 -1.0471 TD (-70)Tj /F5 1 Tf 1.3259 0 TD (8)Tj /F4 1 Tf 0.387 0 TD [(C)-335.5(has)-333.9(been)-339.6(poorly)-338.6(studied.)]TJ /F8 1 Tf -1.7129 -2.1055 TD [(Estimat)-10.3(ion)-334.7(of)-334(epit)-8.2(helial)-342.2(lining)-336.1(fluid)]TJ /F4 1 Tf 1.0015 -1.747 TD [(EL)18.7(F)-318.7(e)0(s)13.9(t)0(i)16.5(m)0(a)17.7(t)0(i)16.5(o)0(n)-308.8(r)0(e)19.2(m)0(a)17.7(i)0(n)13.2(s)-314.9(a)-319.8(c)0(o)15.1(n)0(t)18.9(r)0(o)19.6(v)0(e)15.1(r)0(s)18.4(i)0(a)18.4(l)-321.8(ar)19.2(ea)-309.6([4)13.9(3,)-319.8(120].)]TJ -1.0015 -1.0528 TD [(External)-250.7(marke)-9.4(rs)-237.6(such)-243.7(as)-242.1(inul)-7.8(in)-242.9(or)-242.1(methylene)-249.3(blue)-244.8(are)-243.7(not)]TJ 0 -1.0471 TD [(used)-511.1(in)-510.4(neonat)-7.8(es.)-510(Inter)-9.8(nal)-511.5(markers)-514.4(incl)-8.2(ude)-509.2(urea)-511.9([1)25.3(16,)]TJ 0 -1.0528 TD [(1)27.7(17],)-470.9(albumen)-472.6([120],)-474(tota)-8.2(l)-469.8(protein)-473(and)-469.3(secret)-11(ory)-470.5(com-)]TJ 0 -1.0471 TD [(ponent)-291.9(of)-287.7(immunog)-8.3(lobul)-8.6(in)-282.7(\(Ig\)A)-291.4(\(scIg)-8.6(A\))-285.9([120].)-291.9(Bec)-7.8(ause)]TJ 0 -1.0528 TD [(of)-333.2(capillary)-341.2(leak)-330.6(in)-334(many)-331(respi)-9.4(ratory)-334.9(disord)-9.7(ers,)-330.2(album)-9.7(en)]TJ 0 -1.0471 TD [(and)-298.6(prote)-8.2(in,)-300.2(are)-300.6(unsui)-7.4(table)-305.3(to)-299.8(estimate)-309(ELF)69.9(.)-296.3(Ure)-8.2(a)-297.1(i)0(s)-301(easy)]TJ 0 -1.0527 TD [(to)-214.5(meas)-8.2(ure.)-215.2(Howev)-12.1(er)30.6(,)-210.9(i)0(t)-216.8(may)-222.1(dif)10.8(fuse)-222.5(across)-219.9(the)-215.6(epith)-9(elium)]TJ 0 -1.0471 TD [(durin)-7.8(g)-450.3(the)-454.6(procedu)-7.7(re,)-453.5(whi)-8.6(ch)-451.5(may)-455.4(influence)-464(the)-454.6(results)]TJ 0 -1.0527 TD [([44,)-243.3(121].)-249.7(G)]TJ 6.974 0 0 6.974 106.639 276.435 Tm 0.0001 Tc (RIGG)Tj /F8 1 Tf 9.963 0 0 9.963 125.972 276.435 Tm 0 Tc [(et)-243.3(al.)]TJ /F4 1 Tf 2.2136 0 TD [([1)25.3(14])-242.9(did)-243.7(not)-243.7(show)-247.2(any)-241.7(differences)]TJ -9.0081 -1.0471 TD [(for)-603(ELF)-601.6(between)-610.4(the)-596.9(firs)-9.8(t)-595(a)0(n)-7.6(d)-598.3(second)-604.1(aliquots)-606.7(thus)]TJ 0 -1.0527 TD [(sugges)-7.4(ting)-531.3(that)-531.7(signifi)-9.6(cant)-529.8(diffusion)-528.2(of)-532.3(urea)-529(does)-533.9(not)]TJ 0 -1.0471 TD [(occur)45(.)-302(The)-308.4(use)-305.5(of)-304.7(scIgA)-306.8(has)-305.5(some)-309.4(merits)-311.6([120].)-309(scIgA)-306.8(is)]TJ 0 -1.0528 TD [(secret)-11(ed)-332(by)-337.3(the)-340.8(epitheli)-10.7(um,)-335.1(is)-340.8(thought)-340.1(to)-334(n)-6.5(o)0(t)-334(b)0(e)-337.7(affected)]TJ 0 -1.0471 TD [(by)-388.5(gesta)-8.2(tion,)-395.2(age,)-390.4(lung)-392.4(injury)-397.9(or)-390.1(permeabil)-11.9(ity)-388.3(and)-395.3(does)]TJ 0 -1.0528 TD [(not)-260.7(requi)-8.2(re)-259.6(a)-257.2(serum)-266.2(samp)-7.8(le.)-260.8(Wheth)-7.8(er)-259.6(scIgA)-261.3(product)-9.7(ion)-260.7(is)]TJ 0 -1.0471 TD [(affected)-206.8(by)-212.1(lung)-210.3(inju)-7.8(ry)59.5(,)-205.2(wher)-9(e)-206(epit)-8.2(helial)-217.4(cell)-213.5(damage)-213.8(may-)]TJ 0 -1.0527 TD [(be)-406(e)-6.9(xtensive,)-411.6(is)-409.1(unknow)-8.5(n.)-405.2(Di)-7.8(rect)-411.9(compa)-7.8(risons)-409.3(betw)-9.8(een)]TJ 0 -1.0471 TD [(urea)-244.5(and)-236(scIgA)-244.3(h)-6.5(ave)-236.4(not)-243.7(been)-242.9(performed)-248.9(to)-237.2(estimat)-11.9(e)-240.2(ELF)69.9(.)]TJ /F8 1 Tf 0 -2.1055 TD [(Recomm)-11.7(endations)]TJ /F4 1 Tf 1.0015 -1.7527 TD [(1\))-515.3(NB)18.3(-B)14.9(AL)-516(sh)20(ou)21.2(ld)-516.1(be)-514.1(pe)20.8(rf)18(or)14(me)17.7(d)-524.3(i)0(n)-510.4(a)-524.7(st)17.7(an)20.8(da)15.1(rd)19.6(iz)18.4(ed)]TJ -1.0015 -1.0471 TD [(ma)17.7(nn)15.5(er)70.5(.)-262.2(F)0(o)16.3(r)-252.7(ge)15.1(ne)20.8(ra)13.5(li)16.5(ze)20.4(d)-268.2(l)13.9(un)15.5(g)-262.5(d)0(i)13.2(s)0(e)19.6(a)0(s)19.6(e)0(,)-257.6(t)0(h)18.9(e)-262.9(in)13.2(fa)19.2(nt)-254.3(sh)14.4(ou)21.2(ld)-254.3(be)]TJ 0 -1.0528 TD [(su)20(pi)13.2(ne)-451.5(wi)14.9(th)-453.5(th)18.9(e)-462.1(h)0(e)15.1(a)0(d)-451.5(t)0(u)18.9(r)0(n)13.9(e)0(d)-451.5(t)0(o)-453.5(t)0(h)18.9(e)-467.8(le)18.4(ft)-455.1(an)20.8(d)-461.7(i)0(n)13.2(s)0(p)20(i)0(r)17.3(a)0(t)18.4(o)0(r)13.9(y)]TJ 0 -1.0471 TD [(ox)15.5(yg)21.2(en)-599.4(fr)12.4(ac)20.4(ti)16.5(on)-599(\(F)]TJ 7.97 0 0 7.97 137.65 112.535 Tm [(I,)24.3(O)]TJ 7.472 0 0 6.641 147.685 110.891 Tm (2)Tj 9.963 0 0 9.963 151.313 112.535 Tm [(\))-605.6(i)0(n)13.2(c)0(r)19.2(e)0(a)14.7(s)0(e)19.6(d)-609.7(to)-601.4(ma)17.7(in)18.9(ta)18.4(in)-601.4(ox)15.5(yg)21.2(en)]TJ 15.8596 66.1583 TD [(sa)13.9(tu)18.9(ra)19.2(ti)16.5(on)]TJ /F9 1 Tf 4.1598 0 TD (>)Tj /F4 1 Tf 0.5406 0 TD [(90)15.5(%;)-336.3(2\))-333.2(tw)14.9(o)-342.2(a)0(l)12.8(i)0(q)18.9(u)0(o)21.2(t)0(s)-340.8(1)-342.2(mL)]TJ /F10 1 Tf 10.9998 0.1992 TD (.)Tj /F4 1 Tf 0.2731 -0.1992 TD (kg)Tj 7.472 0 0 6.641 478.261 776.069 Tm (-1)Tj 9.963 0 0 9.963 487.729 771.647 Tm [(ea)20.4(ch)-337.7(of)-333.2(no)15.5(r)20.4(-)]TJ -17.9082 -1.0471 TD [(ma)12(l-)17.3(sa)19.6(li)16.5(ne)-252.3(at)-254.7(ro)19.6(om)-255.1(te)18.5(mp)18.1(er)13.5(at)18.4(ur)19.6(e)-262.9(s)0(h)14.3(o)0(u)21.2(l)0(d)-254.3(b)0(e)-252.3(i)0(n)18.9(s)0(t)17.7(i)0(l)16.5(l)0(e)18.4(d)-262.5(wi)14.9(th)-254.3(an)]TJ 0 -1.0528 TD [(en)15.1(dh)15.5(ol)18.9(e)-354(c)0(a)14.7(t)0(h)18.9(e)0(t)18.4(e)0(r)-350.7(o)0(f)-344.6(s)0(i)17.7(z)0(e)-349.5(F)11.4(G)-357.5(5)0(\261)15.5(8)-353.6(wh)17.3(ic)12.8(h)-353.6(i)0(s)-346.5(w)0(e)16.9(d)0(g)15.5(e)0(d)-343.4(i)0(n)-351(t)0(h)18.9(e)]TJ 0 -1.0471 TD [(ri)17.3(gh)15.5(t)-350.3(l)0(o)18.8(w)0(e)16.9(r)-349.5(lo)18.9(be)15.1(s.)-338.1(Fl)19.6(ui)13.2(d)-347.9(s)0(h)20(o)0(u)15.5(l)0(d)-339.7(b)0(e)-337.7(r)0(e)13.5(c)0(o)20.8(v)0(e)15.1(r)0(e)19.2(d)-347.9(wi)14.9(th)-339.7(a)-348.3(c)0(o)15.1(n)0(-)]TJ 0 -1.0528 TD [(st)17.7(an)15.1(t)-236.5(s)0(u)20(c)0(t)18.5(i)0(o)13.2(n)-234.1(pr)19.6(es)13.9(su)20(re)-225.5(of)-225.1(5\261)15.5(10)-223.5(kP)16.3(a;)-226.2(3\))-230.7(t)13.9(h)0(e)-229.6(r)0(e)19.2(t)0(u)18.9(r)0(n)13.9(e)0(d)-223.9(a)0(l)12.8(i)13.9(qu)15.5(ot)18.9(s)]TJ 0 -1.0471 TD [(ca)14.7(n)-484.5(b)0(e)-474.3(p)0(o)15.5(o)0(l)18.9(e)0(d)-474.3(u)0(n)21.2(l)0(e)12.8(s)0(s)-470.5(c)0(e)14.7(n)0(t)18.9(r)0(a)13.5(l)-481.2(or)-475.4(pe)15.1(ri)17.3(ph)21.2(er)13.5(al)-470.9(ai)18.5(rw)15.7(ay)15.1(s)-485.7(a)0(r)19.2(e)]TJ 0 -1.0528 TD [(sp)14.4(ec)20.4(if)17.3(ic)12.8(al)18.4(ly)-516.1(be)20.8(in)13.2(g)-524.3(i)0(n)18.9(v)0(e)15.1(s)0(t)17.7(i)0(g)18.9(a)0(t)18.5(e)0(d)15.1(;)-526.7(4\))-515.3(th)18.8(e)-524.7(v)0(o)15.5(l)0(u)18.9(m)0(e)-517.2(o)0(f)-509.6(f)0(l)17.3(u)0(i)18.8(d)]TJ 0 -1.0471 TD [(in)13.2(st)17.7(il)16.5(l)13.9(e)0(d)-371.8(a)0(n)20.8(d)-376.3(re)13.5(co)20.8(ve)15.1(re)19.2(d)-376.3(s)0(h)14.4(o)0(u)21.2(l)0(d)-368.1(b)0(e)-366.1(r)0(e)13.5(c)0(o)20.8(r)0(d)13.9(e)0(d)-366.1(a)0(n)20.8(d)-376.3(re)13.5(po)21.2(rt)17.3(ed)15.1(.)]TJ 0 -1.0527 TD [(Wh)14.4(il)16.5(e)-450.7(f)0(i)17.3(l)0(t)16.5(r)0(a)13.5(t)13.9(io)13.2(n)-450.3(t)0(h)18.9(r)0(o)13.9(u)0(g)21.2(h)-456(ga)20.8(uz)15.1(e)-450.7(i)0(s)-443.3(c)0(o)15.1(n)0(s)20(i)0(d)18.9(e)0(r)13.5(e)0(d)-440.1(a)-456.4(st)17.7(an)20.8(da)15.1(rd)]TJ T* [(pr)13.9(oc)20.8(ed)15.1(ur)19.6(e)-308.5(i)0(n)-294.1(o)0(l)13.2(d)0(e)20.8(r)-309.7(ch)20.8(il)16.5(dr)19.6(en)15.1(,)-307.7(i)0(t)-296.5(i)0(s)-295.3(g)0(e)15.1(n)0(e)20.8(r)0(a)13.5(l)0(l)16.5(y)-302.4(un)15.5(ne)20.8(ce)14.7(ss)18.8(ar)19.2(y)-308.1(i)0(n)]TJ 0 -1.0471 TD [(ne)15.1(on)15.5(at)18.4(es)19.6(;)-714.5(5)0(\))-703.1(u)0(n)21.2(t)0(i)16.5(l)-714.5(th)18.8(e)-712.5(m)0(o)18.1(s)0(t)-705(a)0(p)15.1(p)0(r)19.6(o)0(p)15.5(r)0(i)17.3(a)0(t)18.5(e)-712.5(me)17.7(th)18.9(od)-707.2(fo)19.6(r)]TJ 0 -1.0528 TD [(ac)14.7(cu)15.1(ra)19.2(te)18.4(ly)-436.4(es)13.9(ti)22.2(ma)12(ti)16.5(ng)-434(EL)18.7(F)-449.5(i)0(s)-437.6(d)0(e)20.8(t)0(e)18.4(r)0(m)16.5(i)0(n)13.2(e)0(d)20.8(,)-444.2(re)13.5(su)20(lt)16.5(s)-445.8(s)0(h)14.3(o)0(u)21.2(l)0(d)]TJ 0 -1.0471 TD [(be)-314.9(re)13.5(po)21.2(rt)17.3(ed)-320.6(as)-310.4(a)-325.5(c)0(o)15.1(n)0(c)20.8(e)0(n)15.1(t)0(r)17.3(a)0(t)18.5(i)0(o)18.9(n)-325.1(pe)15.1(r)-321(m)0(i)15.7(l)0(l)16.5(i)0(l)16.5(i)0(t)16.5(r)0(e)19.2(.)]TJ /F8 1 Tf 0 -2.1624 TD [(Ar)27.3(eas)-339.6(for)-335.9(further)-337.2(study)]TJ /F4 1 Tf 1.0015 -1.7527 TD [(1\))-327.5(th)18.9(e)-331.2(r)0(e)13.5(p)0(r)19.6(o)0(d)15.5(u)0(c)20.8(i)0(b)13.2(i)13.9(li)16.5(ty)-328.3(of)-321.8(th)18.8(e)-336.9(r)0(e)19.2(c)0(o)20.8(v)0(e)15.1(r)0(y)-321.8(o)0(f)-327.5(B)0(A)18.3(L)0(F)-323.5(a)0(n)15.1(d)-330.8(it)16.5(s)]TJ -1.0015 -1.0471 TD [(co)15.1(ns)20(ti)16.5(tu)18.9(en)15.1(ts)-204.3(fr)18(om)-209.5(th)18.9(e)-211.7(s)0(a)13.9(m)0(e)-204.2(s)0(i)17.7(t)0(e)-203.5(o)0(r)-208(f)0(r)18(o)0(m)-209.5(d)0(i)18.9(f)20.4(fe)19.2(re)13.5(nt)-203.1(si)17.7(te)18.5(s)-218.2(i)0(s)-204.3(n)0(o)21.2(t)]TJ 0 -1.0527 TD [(kn)15.5(ow)17.3(n;)-237.2(2\))-236.4(it)-239.6(is)-238.4(un)21.2(kn)15.5(ow)17.3(n)-245.5(w)0(h)17.3(e)0(t)18.4(h)0(e)15.1(r)-241.4(re)13.5(pe)20.8(at)12.8(ed)-235.2(sa)19.6(mp)12.4(l)13.9(i)0(n)13.2(g)-245.5(of)-236.4(th)18.9(e)]TJ 0 -1.0471 TD [(sa)13.9(me)-192.9(si)17.7(te)-197.8(ov)21.2(er)-197(a)-206(p)0(e)20.8(r)0(i)17.3(o)0(d)-195(o)0(f)-196.6(s)0(e)19.6(v)0(e)20.8(r)0(a)13.5(l)-202.3(we)16.9(ek)15.1(s)-201.1(r)0(e)19.2(s)0(u)14.4(l)0(t)16.5(s)-201.1(in)-197.4(an)20.8(y)-205.6(l)0(o)18.8(c)0(a)20.4(l)]TJ 0 -1.0528 TD [(cl)12.8(in)18.9(ic)18.4(al)18.4(,)-239.4(p)0(a)20.8(t)0(h)18.8(o)0(l)13.2(o)0(g)21.2(i)0(c)18.4(a)0(l)-231.9(o)0(r)-225.1(r)0(a)13.5(d)0(i)18.9(o)0(g)15.5(r)0(a)19.2(p)0(h)21.2(i)0(c)-231.9(c)0(h)20.8(a)0(n)15.1(g)0(e)15.1(s)0(;)-227(3)0(\))-225.1(r)0(e)13.5(f)0(e)19.2(r)0(e)19.2(n)0(c)15.1(e)]TJ 0 -1.0471 TD [(va)15.1(lu)18.8(es)-384.4(fo)19.6(r)-395(h)0(e)15.1(a)0(l)18.4(t)0(h)18.9(y)-393.4(te)18.4(rm)-387.5(an)20.8(d)-393.4(p)0(r)19.6(e)0(t)12.8(e)0(r)19.2(m)-390.9(in)13.2(fa)19.2(nt)18.9(s)-394.6(a)0(r)19.2(e)-393.8(un)15.5(av)20.8(ai)18.4(l-)]TJ 0 -1.0528 TD [(ab)15.1(le)18.4(;)-282(4)0(\))-276.3(c)0(o)15.1(m)0(p)18.1(a)0(r)19.2(i)0(s)17.7(o)0(n)-274.7(b)0(e)15.1(t)0(w)20.6(e)0(e)14.7(n)-285.3(t)13.9(h)0(e)-275.1(u)0(s)14.4(e)-280(of)-276.3(ur)19.6(ea)-275.5(an)15.1(d)-279.6(s)0(c)13.9(I)0(g)19.6(A)-283.5(to)]TJ 0 -1.0471 TD [(es)13.9(ti)16.5(ma)17.7(te)-323(EL)18.7(F)-335.7(i)0(s)-323.8(u)0(r)31(g)0(e)15.1(n)0(t)18.9(l)0(y)-322.6(r)0(e)13.5(q)0(u)21.2(i)0(r)17.3(e)0(d)15.1(;)-333.2(5\))-321.8(th)18.8(e)-336.9(e)0(f)30.6(f)0(e)13.5(c)0(t)18.5(s)-332(of)-321.8(us)14.4(in)18.8(g)]TJ 0 -1.0527 TD [(ga)15.1(uz)15.1(e)-319.8(o)0(r)-316.1(m)0(u)18.1(c)0(o)15.1(l)0(y)18.9(t)0(i)16.5(c)0(s)-316.1(o)0(n)-314.5(m)0(u)18.1(c)0(o)15.1(u)0(s)20(y)-325.1(sa)19.6(mp)12.4(le)18.4(s)-320.6(a)0(r)13.5(e)-325.5(un)21.2(kn)15.5(ow)17.3(n.)]TJ /F6 1 Tf 7.8302 -2.1624 TD [(Research)-330.7(studie)-8.6(s)]TJ /F4 1 Tf -6.8287 -1.747 TD [(Th)17.1(e)-223.1(m)0(a)17.7(i)0(n)-214.5(o)0(b)21.2(j)0(e)18.4(c)0(t)12.8(i)0(v)18.8(e)0(s)-208(o)0(f)-213.7(B)0(A)12.6(L)-215.4(re)13.5(se)19.6(ar)13.5(ch)-206.8(st)17.7(ud)15.5(ie)18.4(s)-218.2(i)0(n)-214.5(c)0(h)15.1(i)13.9(ld)13.2(re)19.2(n)]TJ -1.0015 -1.0528 TD [(ar)13.5(e)-342.6(t)0(o)-334(i)0(m)15.7(p)0(r)19.6(o)0(v)15.5(e)-342.6(th)18.9(e)-342.6(m)0(a)12(n)0(a)20.8(g)0(e)15.1(m)0(e)17.7(n)0(t)-334(o)0(f)-333.2(l)0(u)13.2(n)0(g)-331.6(d)0(i)18.9(s)0(e)13.9(a)0(s)19.6(e)0(s)19.6(,)-341.8(to)-339.7(pr)19.6(o-)]TJ 0 -1.0471 TD [(gr)13.9(es)19.6(s)-388.9(i)0(n)-379.5(t)0(h)18.8(e)-388.1(un)15.5(de)15.1(rs)18.4(ta)18.4(nd)15.5(i)13.9(n)0(g)-382.8(o)0(f)-378.7(l)0(u)18.9(n)0(g)-382.8(d)0(i)18.9(s)0(e)19.6(a)0(s)13.9(e)-388.1(pa)20.8(th)18.9(og)15.5(en)15.1(es)19.6(is)17.7(,)]TJ 0 -1.0528 TD [(an)15.1(d)-262.5(t)0(o)-248.6(a)0(l)18.5(l)0(o)13.2(w)-260.8(id)18.9(en)15.1(ti)22.2(fi)17.3(ca)14.7(ti)16.5(on)-251.9(of)-253.5(pa)20.8(ti)16.5(en)15.1(ts)-249.8(at)-254.7(ri)17.3(sk)-247.4(fo)13.9(r)-258.4(t)0(h)18.9(e)-262.9(de)15.1(ve)20.8(l-)]TJ 0 -1.0471 TD [(op)15.5(me)17.7(nt)-271.4(of)-264.9(re)13.5(sp)20(ir)17.3(at)18.4(or)14(y)-273.9(d)0(i)18.8(s)0(e)14(a)0(s)19.6(e)0(s)-264.9(a)0(n)15.1(d)-273.9(pr)13.9(ed)20.8(ic)12.8(ti)22.2(on)-269(of)-270.6(ou)21.2(tc)12.8(om)18.1(e.)]TJ /F8 1 Tf 0 -2.1624 TD [(Manage)-7.3(ment)-339.8(of)-339.6(lung)-335.5(diseases)-342.9(in)-334(childr)23.2(en)]TJ /F4 1 Tf 1.0015 -1.7527 TD [(Fu)16.3(tu)18.8(re)-339.3(di)18.9(re)19.2(ct)18.4(io)13.2(ns)-332.8(ne)20.8(ed)-332(to)-334(fo)13.9(cu)20.8(s)-343.4(o)0(n)-331.6(t)0(h)13.2(e)-342.6(ch)20.8(ar)13.5(ac)20.4(te)18.5(ri)17.3(za)14.7(ti)16.5(on)]TJ -1.0015 -1.0471 TD [(of)-264.9(ma)12(rk)19.6(er)19.2(s)-275.1(o)0(f)-264.9(a)0(c)14.7(u)0(t)18.9(e)-274.3(in)18.9(fl)17.3(am)17.7(ma)12(t)13.9(i)0(o)13.2(n)-273.9(as)-264.9(w)12.4(e)0(l)12.8(l)-270.6(as)-264.9(of)-264.9(ma)17.7(rk)13.9(er)19.2(s)-275.1(o)0(f)]TJ 0 -1.0528 TD [(re)13.5(pa)20.8(ir)-318.5(pr)19.6(oc)15.1(es)19.6(se)14(s.)]TJ /F8 1 Tf 0 -2.2079 TD [(Marke)-8.2(rs)-242.9(of)-242.9(acute)-246(inflam)-10(mation.)]TJ /F4 1 Tf 12.798 0 TD [(E)12.2(f)14.7(fo)19.6(rt)17.3(s)-229.6(s)0(h)14.4(o)0(u)21.2(l)0(d)-225.8(b)0(e)-218.2(m)0(a)17.7(d)0(e)-223.9(t)0(o)]TJ -12.798 -1.0527 TD [(in)13.2(te)18.4(gr)19.6(at)18.4(e)-217.4(t)0(h)18.9(e)-217.4(co)15.1(nc)20.8(ep)15.1(t)-213.7(o)0(f)-208(b)0(a)15.1(l)0(a)18.5(n)0(c)15.1(e)-217.4(be)20.8(tw)14.9(ee)20.4(n)-217(p)0(r)13.9(o)0(-)19.6(i)0(n)18.8(f)0(l)17.3(a)0(m)17.7(m)0(a)12(t)0(o)18.9(r)0(y)]TJ 0 -1.0471 TD [(an)15.1(d)-717.8(a)0(n)20.8(t)0(i)16.5(-)0(i)17.3(n)0(f)14(l)0(a)18.4(m)0(m)14.9(a)0(t)18.4(o)0(r)19.6(y)-717.8(mo)18.1(le)12.8(cu)20.8(le)18.4(s,)-708(an)15.1(d)-717.8(t)13.9(o)-717.8(c)0(o)15.1(-)0(o)19.6(r)0(d)13.9(i)0(n)18.8(a)0(t)18.4(e)]TJ 0 -1.0527 TD [(me)12(as)19.6(ur)19.6(em)12(en)20.8(ts)-494.5(of)-492.5(th)18.9(es)19.6(e)-501.9(f)0(a)19.2(c)0(t)12.8(o)0(r)19.6(s)0(.)-491.7(T)12.2(hi)13.2(s)-497(i)0(n)18.9(v)0(o)15.5(l)0(v)18.8(e)0(s)-492.5(p)0(r)19.6(o)0(-)-492.5(a)0(n)15.1(d)]TJ 0 -1.0471 TD [(an)15.1(ti)16.5(-i)17.3(nf)19.6(la)18.4(mm)14.9(at)12.8(or)19.6(y)-325.1(c)0(y)15.1(t)0(o)18.9(k)0(i)18.9(n)0(e)15.1(s)-320.6([1)13.9(22)21.2(,)-336.1(123].)-340.8(Other)-342.2(molecules)]TJ 0 -1.0015 TD [(such)-482.7(as)-481.1(oxygen)-483(radic)-8.6(als,)-484.7(platelet)-489.2(activating)-485(facto)-8.6(r)-480.4(and)]TJ T* [(met)-8.2(abolites)-476.2(of)-464.1(arachi)-9.8(donic)-467.5(acid,)-473.2(proteases,)-475.7(coagul)-7.8(ation)]TJ T* [(cascade)-429.7(and)-423.8(compl)-9.7(ement)-425.6(play)-421.2(an)-423(impo)-8.6(rtant)-426(role)-422.8(in)-419.3(the)]TJ 0 -0.9958 TD [(infl)-9.4(ammator)-10.7(y)-342.2(respon)-7.8(se)-350.3([124,)-352.1(125].)-346.5(The)-6.8(se)-344.6(facto)-8.6(rs)-345.7(can)-350.2(be)]TJ 0 -1.0015 TD 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[(.)-363.3(The)-368.5(physiologic)]TJ -15.6619 -1.1191 TD [(consequences)-406.1(of)-410.9(saline)-407.1(lobar)-405.3(lavages)-410.3(in)-406(healthy)-411.7(human)]TJ 0 -1.1128 TD (adults.)Tj /F8 1 Tf 2.9465 0 TD [(Am)-334.6(Rev)-330.3(Respir)-340.1(Dis)]TJ /F4 1 Tf 8.0933 0 TD [(1983;)-328.6(127:)-335.5(695\261701.)]TJ -13.5563 -1.1128 TD [(33.)-1266.5(Ratjen)-296.8(F)71.5(,)-293.8(Bredendiek)-299.4(M,)-293.9(Brendel)-301.2(M,)-293.9(Meltzer)-299.3(J,)-300.8(Costabel)]TJ 2.5165 -1.1128 TD [(U.)-347.1(Differential)-350.3(cytology)-348.2(of)-347.7(bronchoalveolar)-352.2(lavage)-352.7(fluid)]TJ 0 -1.1192 TD [(in)-330.2(normal)-337(children.)]TJ /F8 1 Tf 8.0617 0 TD [(Eur)-332(Respir)-333.8(J)]TJ /F4 1 Tf 5.5073 0 TD [(1994;)-334.9(7:)-330.2(1865\2611870.)]TJ -16.0855 -1.1065 TD [(34.)-1266.5(Midulla)-244.3(F)77.8(,)-243.2(V)54.1(illani)-246.5(A,)-239.6(Merolla)-242.7(R,)-241.1(Bjermer)-240.8(L,)-239.8(Sandstrom)-246.8(T)72.9(,)]TJ 2.5165 -1.1192 TD 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[(36.)-1266.5(The)-273.6(BAL)-281.9(Cooperative)-276(Group)-279.9(Steering)-278(Committee.)-279(Bron-)]TJ 2.5165 -1.1128 TD [(choalveolar)-511.4(lavage)-517.1(constituents)-511.2(in)-513.5(healthy)-512.9(individuals,)]TJ 0 -1.1192 TD [(idiopathic)-478.2(pulmonary)-474.6(fibrosis)-472.9(and)-472.5(selected)-481.5(comparison)]TJ 0 -1.1128 TD (groups.)Tj /F8 1 Tf 3.2879 0 TD [(Am)-334.6(Rev)-336.6(Respir)-333.8(Dis)]TJ /F4 1 Tf 8.0933 0 TD [(1990;)-334.9(141:)-329.1(S169\261S201.)]TJ -13.8978 -1.1128 TD [(37.)-1266.5(Ratjen)-334.8(F)71.5(,)-331.7(Bredendiek)-337.4(M,)-331.9(Zheng)-335.8(L,)-334.7(Brendel)-332.8(M,)-338.2(Costabel)]TJ 2.5165 -1.1128 TD [(U.)-328.2(L)47.6(ymphocytes)-328.7(subsets)-331(in)-330.2(bronchoalveolar)-339.6(lavage)-327.4(fluid)]TJ 0 -1.1192 TD [(of)-550.1(children)-551.3(without)-557(bronchopulmonary)-554(disease.)]TJ /F8 1 Tf 20.5115 0 TD [(Am)-549.5(J)]TJ -20.5115 -1.1128 TD [(Respir)-333.8(Crit)-337.4(Car)33.6(e)-334.4(Med)]TJ /F4 1 Tf 9.2251 0 TD [(1995;)-334.9(152:)-335.5(174\261178.)]TJ -11.7416 -1.1128 TD [(38.)-1266.5(Baughman)-425.7(R,)-424.4(Strohofer)-435.3(S,)-424.6(Kim)-423.4(K.)-429.3(V)104.7(ariation)-433.6(of)-423.6(diff)10.4(er-)]TJ 2.5165 -1.1192 TD [(ential)-395.7(cell)-400.6(counts)-398.6(of)-398.3(bronchoalveolar)-396.5(lavage)-397(fluid.)]TJ /F8 1 Tf 20.9225 0 TD [(Ar)27.9(ch)]TJ -20.9225 -1.1128 TD [(Pathol)-334(Lab)-332.2(Med)]TJ /F4 1 Tf 6.9552 0 TD [(1986;)-334.9(1)32.1(10:)-336(341\261343.)]TJ -9.4717 -1.1128 TD [(39.)-1266.5(Padovan)-367.6(CS,)-359.3(Behr)-370.1(J,)-364(Allmeling)-370.3(AM,)-366.3(T)34.9(ilman)-370.6(Gerlach)-369.1(J,)]TJ 2.5165 -1.1128 TD [(V)123.6(ogelmeier)-552.4(C,)-557.2(Krombach)-551.7(FP)105(.)-553(Immunophenotyping)-557(of)]TJ 0 -1.1192 TD [(lymphocyte)-209.7(subsets)-217.1(in)-210(bronchoalveolar)-213.1(lavage)-213.6(fluid-)-212.3(com-)]TJ 0 -1.1128 TD [(parison)-607.8(of)-600.6(flow)-611.6(cytometric)-611.2(and)-611.6(immunocytochemical)]TJ 0 -1.1192 TD (techniques.)Tj /F8 1 Tf 4.8181 0 TD [(J)-334.4(Immunol)-335.2(Methods)]TJ /F4 1 Tf 8.3842 0 TD [(1992;)-334.9(147:)-335.5(27\26132.)]TJ -15.7188 -1.1128 TD [(40.)-1266.5(Reynolds)-509.8(HY)127.2(,)-508.8(Newball)-506.8(HH.)-508(Analysis)-512(of)-505.8(proteins)-508.5(and)]TJ 2.5165 -1.1128 TD [(respiratory)-262(cells)-262.2(obtained)-259.5(from)-261(human)-264.7(lungs)-260.3(by)-258.2(bronchial)]TJ T* (lavage.)Tj /F8 1 Tf 3.1678 0 TD [(J)-328.1(Lab)-338.5(Clin)-329.9(Med)]TJ /F4 1 Tf 6.7845 0 TD [(1974;)-334.9(84:)-336(559\261574.)]TJ -12.4688 -1.1128 TD [(41.)-1266.5(Marcy)-593(TW)80.4(,)-590.9(Merrill)-600.3(WW)84.7(,)-597.3(Rankin)-591.8(JA,)-594.4(Reynolds)-598.3(HY)120.9(.)]TJ 2.5165 -1.1192 TD [(Limitations)-360.4(of)-354(urea)-352.6(to)-355.5(quantify)-361.8(epithelial)-353.9(lining)-358.2(fluid)-357.3(by)]TJ 0 -1.1128 TD [(bronchoalveolar)-396.5(lavage.)]TJ /F8 1 Tf 10.0534 0 TD [(Am)-397.8(Rev)-393.5(Respir)-390.7(Dis)]TJ /F4 1 Tf 8.3336 0 TD [(1987;)-391.9(135:)]TJ -18.387 -1.1192 TD (1276\2611280.)Tj -2.5165 -1.1065 TD [(42.)-1266.5(W)70.8(ard)-384.9(C,)-392.8(Duddridge)-387.5(M,)-395.1(Fenwick)-385.9(J,)]TJ /F8 1 Tf 17.3248 0 TD [(et)-393.2(al)]TJ /F4 1 Tf 1.8716 0 TD [(.)-388.6(Evaluation)-390.8(of)]TJ -16.6798 -1.1192 TD [(albumin)-388.5(as)-392(a)-385(refenrence)-395(marker)-392.3(of)-385.7(dilution)-391.2(in)-387.1(broncho-)]TJ 0 -1.1128 TD [(alveolar)-418.3(lavage)-422.3(fluid)-414.2(of)-417.3(asthmatic)-422.3(and)-415.6(control)-419.3(subjects.)]TJ /F8 1 Tf 0 -1.1192 TD (Thorax)Tj /F4 1 Tf 3.2057 0 TD [(1993;)-334.9(48:)-336(518\261522.)]TJ -5.7222 -1.1128 TD [(43.)-1266.5(V)123.6(o)0(n)-606(W)32.8(ichert)-609.2(P)103.1(,)-603.6(Joseph)-606.1(K,)-606.4(Mueller)-609.4(B,)-607.8(Franck)-603(WM.)]TJ 2.5165 -1.1128 TD [(Bronchoalveolar)-470.7(lavage.)-475.8(Quantification)-469.9(of)-474.2(intraalveolar)]TJ T* (fluid?)Tj /F8 1 Tf 2.6367 0 TD [(Am)-328.3(Rev)-336.6(Respir)-333.8(Dis)]TJ /F4 1 Tf 8.087 0 TD [(1993;)-334.9(147:)-335.5(148\261152.)]TJ -13.2402 -1.1128 TD [(44.)-1266.5(W)70.8(ard)-384.9(C,)-392.8(Duddridge)-387.5(M,)-388.8(Fenwick)-392.3(J,)]TJ /F8 1 Tf 17.3248 0 TD [(et)-386.8(al)]TJ /F4 1 Tf 1.8653 0 TD [(.)-388.6(The)-393.8(origin)-388.4(of)]TJ -16.6735 -1.1192 TD [(water)-528.4(and)-529.4(urea)-529.6(sampled)-532.6(at)-532.3(bronchoalveolar)-529.2(lavage)-529.8(in)]TJ 0 -1.1128 TD [(asthmatic)-295.8(and)-289.1(control)-299.1(subjects.)]TJ /F8 1 Tf 12.6205 0 TD [(Am)-296.6(Rev)-292.4(Respir)-289.5(Dis)]TJ /F4 1 Tf 7.9289 0 TD (1992;)Tj -20.5495 -1.1192 TD [(146:)-335.5(444\261447.)]TJ -2.5165 -1.1065 TD [(45.)-1266.5(Report)-320.9(of)-322.4(the)-316.8(European)-322.4(T)66.6(ask)-321.9(Force)-319(for)-322.9(noncellular)-324.3(com-)]TJ 2.5165 -1.1192 TD [(ponents)-233.7(of)-227.6(bronchoalveolar)-238.4(lavage:)-234.5(special)-235.7(considerations)]TJ 0 -1.1128 TD [(in)-330.2(children.)]TJ /F8 1 Tf 4.9256 0 TD [(Eur)-332(Respir)-333.8(Rev)]TJ /F4 1 Tf 6.5505 0 TD [(1999;)-334.9(66:)-329.7(1\261157.)]TJ -13.9926 -1.1128 TD [(46.)-1266.5(Stokes)-371.9(DC,)-376.6(Shenep)-374.5(JL,)-373.3(Parham)-377.3(D,)-372.4(Bozeman)-375.4(PM,)-374.2(Mar-)]TJ 2.5165 -1.1192 TD [(ienchek)-345.9(W)83.4(,)-344.4(Mackert)-347.5(PW)85.3(.)-344.4(Role)-346.3(of)-335.1(flexible)-345(bronchoscopy)]TJ 0 -1.1128 TD [(in)-570.4(the)-569.7(diagnosis)-571.4(of)-575.3(pulmonary)-569.5(infiltrates)-578(in)-570.4(pediatric)]TJ 0 -1.1192 TD [(patients)-332.6(with)-341.1(cancer)53.6(.)]TJ /F8 1 Tf 8.5992 0 TD [(J)-334.4(Pediatr)]TJ /F4 1 Tf 4.072 0 TD [(1989;)-334.9(1)32.1(15:)-336(561\261567.)]TJ -15.1876 -1.1065 TD [(47.)-1266.5(de)-264.3(Blic)-272.8(J,)-262.8(Blanche)-274.7(S,)-266.5(Danel)-268.2(C,)-266.4(Le)-267.8(Bourgeo)10.8(is)-268.1(M,)-268.6(Caniglia)]TJ 2.5165 -1.1192 TD [(M,)-300.3(Scheinmann)-306.9(P)103.1(.)-306.4(Bronchoalveolar)-306.3(lavage)-308.5(in)-304.9(HIV)-252.6(infec-)]TJ 0 -1.1128 TD [(ted)-310.5(patients)-313.6(with)-309.5(interstitial)-315.8(pneumonitis.)]TJ /F8 1 Tf 16.7241 0 TD [(Ar)34.3(ch)-308.6(Dis)-315.2(Child)]TJ /F4 1 Tf -16.7241 -1.1192 TD [(1989;)-334.9(64:)-329.6(1246\2611250.)]TJ -2.5165 -1.1065 TD [(48.)-1266.5(McCubbin)-243.7(MM,)-243.5(T)28.6(rigg)-241.6(ME,)-240(Hendricker)-248.4(CM,)-241.2(W)70.8(agener)-243.8(JS.)]TJ 2.5165 -1.1191 TD [(Bronchoscopy)-310.1(with)-309.5(bronchoalveolar)-307.9(lavage)-308.5(in)-311.2(the)-310.5(evalu-)]TJ 0 -1.1128 TD [(ation)-299.2(of)-303.5(pulmonary)-297.6(complications)-308.8(of)-297.1(bone)-301.2(marrow)-301(trans-)]TJ 0 -1.1192 TD [(plantation)-212.6(in)-216.4(children.)]TJ /F8 1 Tf 8.8521 0 TD [(Pediatr)-214.3(Pulmonol)]TJ /F4 1 Tf 7.2524 0 TD [(1992;)-214.8(12:)-209.5(43\26147.)]TJ -18.621 -1.1128 TD [(49.)-1266.5(Abadco)-461.4(DL,)-463.9(Amaro-Galvez)-473.6(R,)-462.4(Rao)-464.5(M,)-464.7(Steiner)-468.2(P)103.1(.)-458.2(Ex-)]TJ 2.5165 -1.1128 TD [(perience)-232.3(with)-233.7(flexible)-237.5(fiberoptic)-231.2(bronchoscopy)-235.8(with)-233.7(bron-)]TJ 0 -1.1192 TD [(choalveolar)-378.6(lavage)-371.7(as)-373(a)-372.3(diagnostic)-375.8(tool)-375.8(in)-368.1(children)-380.6(with)]TJ 0 -1.1128 TD (AIDS.)Tj /F8 1 Tf 2.8959 0 TD [(Am)-334.6(J)-328.1(Dis)-340.5(Child)]TJ /F4 1 Tf 6.6707 0 TD [(1992;)-334.9(146:)-335.5(1056\2611059.)]TJ -40.0809 76.3934 TD (228)Tj 7.472 0 0 7.472 266.627 797.499 Tm 0.0001 Tc [(ERS)-340.2(T)73(A)0(SK)-333.6(FORCE)]TJ ET endstream endobj 52 0 obj << /ProcSet [/PDF /Text ] /Font << /F4 7 0 R /F8 16 0 R >> /ExtGState << /GS2 11 0 R >> >> endobj 54 0 obj << /Length 19280 >> stream BT /F4 1 Tf 8.966 0 0 8.966 58.28 772.157 Tm 0 g /GS2 gs 0 Tc [(50.)-1266.5(Kurland)-407.5(G,)-404(Noyes)-406.4(BE,)-402.1(Jaffe)-398.5(R,)-405.5(Atlas)-411.1(AB,)-401.9(Armitage)-411(J,)]TJ 2.5165 -1.1191 TD [(Orenstein)-485.7(DM.)-492.7(Bronchoalveolar)-489.7(lavage)-491.8(and)-485.1(transbron-)]TJ 0 -1.1128 TD [(chial)-444.4(biopsy)-443.1(in)-450.3(children)-443.8(following)-449.4(heart-lung)-450.1(and)-447.2(lung)]TJ 0 -1.1192 TD (transplantation.)Tj /F8 1 Tf 6.4557 0 TD (Chest)Tj /F4 1 Tf 2.5924 0 TD [(1993;)-334.9(104:)-329.1(1043\2611048.)]TJ -11.5646 -1.1065 TD [(51.)-1266.5(Perez)-337.8(CR,)-333.8(W)70.8(ood)-339.9(RE.)-338.9(Update)-337.3(on)-334.1(pediatric)-344.3(flexible)-338.7(bron-)]TJ 2.5165 -1.1192 TD [(choscopy)66.6(.)]TJ /F8 1 Tf 4.2743 0 TD [(Pediatr)-340.8(Clin)-329.9(North)-334.5(Am)]TJ /F4 1 Tf 9.6551 0 TD [(1994;)-334.9(41:)-336(385\261400.)]TJ -16.4459 -1.1128 TD [(52.)-1266.5(Riedler)-215.1(J,)-218.6(Grigg)-212.8(J,)-218.6(Robertson)-213.1(CF)67.3(.)-211.6(Role)-219.8(of)-214.9(bronchoalveolar)]TJ 2.5165 -1.1128 TD [(lavage)-308.5(in)-317.5(children)-311(with)-315.9(lung)-316.5(disease.)]TJ /F8 1 Tf 15.0991 0 TD [(Eur)-313(Respir)-314.8(J)]TJ /F4 1 Tf 5.4504 0 TD (1995;)Tj -20.5495 -1.1192 TD [(8:)-330.2(1725\2611730.)]TJ -2.5165 -1.1065 TD [(53.)-1266.5(Rock)-343.9(MJ.)-351.5(The)-349.5(diagnostic)-350.5(utility)-349.8(of)-347.7(bronchoalveolar)-352.2(lav-)]TJ 2.5165 -1.1192 TD [(age)-238.3(in)-235.3(immunocompetent)-244.7(children)-241.5(with)-240(unexplained)-238.7(infil-)]TJ 0 -1.1128 TD [(trates)-256.3(on)-251.9(chest)-253.5(radiograph.)]TJ /F8 1 Tf 10.7047 0 TD (Pediatrics)Tj /F4 1 Tf 4.3186 0 TD [(1995;)-252.7(95:)-253.8(373\261377.)]TJ -17.5398 -1.1128 TD [(54.)-1266.5(Eber)-419.5(E.)-416.9(Bronchoalveolar)-426.5(lavage)-422.3(in)-418.7(pediatric)-426.5(patients.)]TJ /F8 1 Tf 24.8933 0 TD 0 Tw (J)Tj -22.3768 -1.1191 TD [(Br)34.3(onchology)]TJ /F4 1 Tf 5.4314 0 TD [(1998;)-334.9(5:)-336.5(227\261241.)]TJ -7.9479 -1.1065 TD [(55.)-1266.5(Naber)-475.5(SP)105(.)-477.1(Molecular)-475.4(medicine.)-479(Molecular)-481.7(pathology)-480.5(-)]TJ 2.5165 -1.1192 TD [(diagnosis)-274.2(of)-271.8(infectious)-282(disease.)]TJ /F8 1 Tf 12.6648 0 TD [(N)-276.1(Engl)-275.7(J)-271.2(Med)]TJ /F4 1 Tf 5.836 0 TD [(1994;)-278(331:)]TJ -18.5008 -1.1128 TD (1212\2611215.)Tj -2.5165 -1.1128 TD [(56.)-1266.5(Schluger)-313.5(NW)80.6(,)-312.7(Rom)-321.2(WN.)-314.3(The)-317.9(polymerase)-316.8(chain)-315.5(reaction)]TJ 2.5165 -1.1191 TD [(in)-323.8(the)-323.1(diagnosis)-324.8(and)-320.7(evaluation)-330.2(of)-322.4(pulmonary)-322.9(infections.)]TJ /F8 1 Tf 0 -1.1128 TD [(Am)-328.2(J)-334.4(Respir)-333.8(Crit)-337.4(Car)27.2(e)-328.1(Med)]TJ /F4 1 Tf 11.6468 0 TD [(1995;)-334.9(152:)-335.5(1)32.1(1\26116.)]TJ -14.1633 -1.1128 TD [(57.)-1266.5(Eber)-438.5(E,)-435.8(Zach)-437.3(M.)-439.4(Pediatric)-437.7(flexible)-446.2(fiberoptic)-439.8(broncho-)]TJ 2.5165 -1.1128 TD [(scopy)-510.6(\261)-518(a)0(n)-510.9(analysis)-521.1(of)-512.1(420)-517(investigations.)]TJ /F8 1 Tf 18.6147 0 TD [(W)50.6(ien)-519.1(Klin)]TJ -18.6147 -1.1191 TD [(W)88.5(ochenschr)]TJ /F4 1 Tf 5.1532 0 TD [(1995;)-334.9(107:)-329.1(246\261251.)]TJ -7.6697 -1.1128 TD [(58.)-1266.5(Bjermer)-278.8(L,)-271.5(Rust)-278.1(M,)-275(Heurlin)-277.1(N,)-277.6(Rennard)-273.5(S,)-279.2(Klech)-274.6(H.)-277.6(The)]TJ 2.5165 -1.1128 TD [(clinical)-453.7(use)-448.4(of)-455.2(bronchoalveolar)-453.4(lavage)-453.9(in)-450.3(patients)-452.7(with)]TJ 0 -1.1192 TD [(pulmonary)-329.2(infections.)]TJ /F8 1 Tf 9.1113 0 TD [(Eur)-331.9(Respir)-333.8(J)]TJ /F4 1 Tf 5.5073 0 TD [(1992;)-334.9(2:)-330.2(106\2611)27.8(13.)]TJ -17.1351 -1.1065 TD [(59.)-1266.5(Meduri)-228.4(GU,)-236.1(Beals)-232.9(DH,)-236.1(Maijub)-229.8(AG,)-236.1(Baselski)-234.9(V)123.6(.)-230.5(Protected)]TJ 2.5165 -1.1191 TD [(bronchoalveolar)-307.9(lavage.)-305(A)-312.6(new)-305.1(bronchoscopic)-307(technique)]TJ 0 -1.1128 TD [(to)-393.4(retrieve)-400.3(uncontaminated)-399.3(distal)-397.1(airway)-401.5(secretions.)]TJ /F8 1 Tf 21.4916 0 TD (Am)Tj -21.4916 -1.1192 TD [(Rev)-330.3(Respir)-333.8(Dis)]TJ /F4 1 Tf 6.4304 0 TD [(1991;)-334.9(143:)-335.5(855\261864.)]TJ -8.9469 -1.1128 TD [(60.)-1266.5(Baker)-230.3(AM,)-233.5(Bowton)-228.6(DL,)-230(Haponik)-235.8(EF)74.8(.)-230.5(Decision)-232.3(making)-234.9(in)]TJ 2.5165 -1.1128 TD [(nosocomial)-362.7(pneumonia.)-362.5(An)-362.7(analytic)-362.8(approach)-362.9(to)-361.8(the)-367.4(in-)]TJ 0 -1.1192 TD [(terpretation)-276.5(of)-271.8(quantitative)-277(bronchoscopic)-275.4(cultures.)]TJ /F8 1 Tf 20.5558 0 TD (Chest)Tj /F4 1 Tf -20.5558 -1.1128 TD [(1995;)-328.6(107:)-335.5(85\26195.)]TJ -2.5165 -1.1128 TD [(61.)-1266.5(Jourdain)-575.6(B,)-576.2(Joly-Guillou)-584.2(M-L,)-575.6(Dombret)-586.4(M-C,)]TJ /F8 1 Tf 23.028 0 TD [(et)-576.5(al)]TJ /F4 1 Tf 2.0549 0 TD (.)Tj -22.5665 -1.1128 TD [(Usefulness)-580(of)-575.3(quantitative)-580.5(cultures)-584.1(of)-575.3(BAL)-579(fluid)-578.6(for)]TJ 0 -1.1192 TD [(diagnosing)-297.8(nosocomial)-293.1(pneumonia)-302.7(in)-292.2(ventilated)-300.9(patients.)]TJ /F8 1 Tf T* (Chest)Tj /F4 1 Tf 2.5924 0 TD [(1997;)-328.6(1)32.1(1)25.8(1:)-330.2(41)32.6(1\261418.)]TJ -5.1089 -1.1065 TD [(62.)-1266.5(Speich)-345.2(R,)-348.6(W)32.8(u)]TJ 7.8214 -0.0253 TD (\310)Tj 0.411 0.0253 TD 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[(J)-264.8(Clin)-266.7(Micr)29.8(obiol)]TJ /F4 1 Tf 6.8604 0 TD [(1993;)-265.4(31:)-266.4(3325\2613328.)]TJ -16.5154 -1.1128 TD [(64.)-1266.5(Bretagne)-547.1(S,)-544.8(Costa)-549.3(JM,)-547.5(V)54.1(idaud)-549.7(M,)-546.9(T)34.9(ran)-549.3(J,)-547.4(Nhieu)-546.7(V)123.6(,)]TJ 2.5165 -1.1128 TD [(Fleury-Feith)-322.1(J.)-319.7(Detection)-321.3(of)]TJ /F8 1 Tf 11.5077 0 TD [(T)84.1(oxoplasma)-324.5(gondii)]TJ /F4 1 Tf 7.9605 0 TD [(by)-315.1(com-)]TJ -19.4682 -1.1192 TD [(petitive)-523.5(DNA)-520.5(amplification)-525.9(of)-524.8(bronchoalveolar)-522.9(lavage)]TJ 0 -1.1128 TD (samples.)Tj /F8 1 Tf 3.7685 0 TD [(J)-334.4(Infect)-331(Dis)]TJ /F4 1 Tf 5.0457 0 TD [(1993;)-334.9(168:)-335.5(1585\2611588.)]TJ -11.3307 -1.1128 TD [(65.)-1266.5(Leibovitz)-486.1(E,)-486.4(Pollack)-486.2(H,)-486.2(Rigaud)-490.7(M,)]TJ /F8 1 Tf 18.0203 0 TD [(et)-488(al)]TJ /F4 1 Tf 1.9664 0 TD [(.)-483.5(Polymerase)]TJ -17.4702 -1.1192 TD [(chain)-372.4(reaction)-374(is)-375.6(more)-373.6(sensitive)-371.2(than)-373.2(standard)-379.4(cytologic)]TJ 0 -1.1128 TD [(stains)-623.5(in)-621(detecting)]TJ /F8 1 Tf 8.5106 0 TD [(Pneumocystis)-626.8(carinii)]TJ /F4 1 Tf 9.3326 0 TD [(in)-621(broncho-)]TJ -17.8433 -1.1192 TD [(alveolar)-532.1(lavages)-530.5(from)-532.9(human)-530.2(immunodeficiency)-533.9(virus)]TJ 0 -1.1128 TD [(type)-550.2(1-infected)-557.3(infants)-559.5(and)-554.7(children)-557.6(with)-556.1(pneumonia.)]TJ /F8 1 Tf 0 -1.1192 TD [(Pediatr)-334.4(Infect)-331(Dis)-340.5(J)]TJ /F4 1 Tf 8.3462 0 TD [(1995;)-334.9(14:)-336(714\261716.)]TJ -10.8628 -1.1065 TD [(66.)-1266.5(Delacourt)-598.1(C,)-595.1(Poveda)-595.7(JD,)-594.4(Chureau)-596(C,)]TJ /F8 1 Tf 19.4556 0 TD [(et)-595.5(al)]TJ /F4 1 Tf 2.0739 0 TD [(.)-597.3(Use)-597.1(of)]TJ -19.013 -1.1191 TD [(polymerase)-569.7(chain)-568.5(reaction)-576.4(for)-569.5(improved)-570(diagnosis)-577.7(of)]TJ 0 -1.1128 TD [(tuberculosis)-332.7(in)-336.5(children.)]TJ /F8 1 Tf 10.0345 0 TD [(J)-334.4(Pediatr)]TJ /F4 1 Tf 4.072 0 TD [(1995;)-334.9(126:)-335.5(703\261709.)]TJ -16.6229 -1.1128 TD [(67.)-1266.5(Kessler)-207.3(HH,)-210.8(Dodge)-209.2(DE,)-211(Pierer)-208(K,)]TJ /F8 1 Tf 15.9464 0 TD [(et)-209.8(al)]TJ /F4 1 Tf 1.6882 0 TD [(.)-211.6(Rapid)-206.7(detection)-216.9(of)]TJ -15.1181 -1.1192 TD [(Mycoplasma)-272.2(pneumoniae)-276.7(by)-270.9(an)-270.7(assay)-271.3(based)-276.4(on)-270.9(PCR)-272.1(and)]TJ 0 -1.1128 TD [(probe)-377.6(hybridization)-382.8(in)-380.8(a)-378.7(nonradioactive)-379.4(microwell)-387.6(plate)]TJ 0 -1.1192 TD (format.)Tj /F8 1 Tf 3.2057 0 TD [(J)-334.4(Clin)-329.9(Micr)23.5(obiol)]TJ /F4 1 Tf 7.069 0 TD [(1997;)-334.9(35:)-329.6(1592\2611594.)]TJ -12.7912 -1.1065 TD [(68.)-1266.5(Goyal)-338(M,)-344.5(Shaw)-347.4(RJ,)-342.9(Banerjee)-344.5(DK,)-343.6(Coker)-344.3(RJ,)-342.9(Robertson)]TJ 2.5165 -1.1192 TD [(BD,)-433.5(Y)98.4(oung)-434.3(DB.)-433.5(Rapid)-434.3(detection)-438.2(of)-429.9(multidrug-resistant)]TJ 0 -1.1128 TD (tuberculosis.)Tj /F8 1 Tf 5.3618 0 TD [(Eur)-331.9(Respir)-333.8(J)]TJ /F4 1 Tf 5.5073 0 TD [(1997;)-334.9(10:)-329.6(1)32.1(120\2611)27.8(124.)]TJ -13.3856 -1.1128 TD [(69.)-1266.5(Chaske)-375.4(J,)-383(T)34.9(rouillet)-385.6(J-L.)-380.1(Nosocomial)-378.6(pneumonia:)-380.5(guide-)]TJ 30.5144 73.5671 TD [(lines)-262.4(for)-266(initial)-267.4(management)-272(and)-263.8(empirical)-264(treatment.)]TJ /F8 1 Tf 21.3335 0 TD (Eur)Tj -21.3335 -1.1191 TD [(Respir)-333.8(Mon)]TJ /F4 1 Tf 5.071 0 TD [(1997;)-334.9(3:)-330.2(101\2611)27.8(17.)]TJ -7.5875 -1.1065 TD [(70.)-1266.5(A)104.7(T)0(S)-216(Committee)-225.5(on)-214(Health)-225.9(Care)-218.2(Policy)-215.1(and)-219.6(Practice.)-223.5(Clin-)]TJ 2.5165 -1.1192 TD [(ical)-350(role)-348.9(of)-347.7(bronchoalveolar)-345.9(lavage)-352.7(in)-342.8(adults)-350.4(with)-347.5(pulm-)]TJ 0 -1.1128 TD [(onary)-276.4(disease)-285.1(\(A)104.2(TS)-279.3(statement\).)]TJ /F8 1 Tf 12.6774 0 TD [(Am)-277.7(Rev)-279.7(Respir)-276.9(Dis)]TJ /F4 1 Tf 7.872 0 TD (1990;)Tj -20.5495 -1.1192 TD [(142:)-335.5(481\261486.)]TJ -2.5165 -1.1128 TD [(71.)-1266.5(Pinar)-315.3(A,)-321.8(Ahkee)-316.4(S,)-317.1(Miller)-321.7(RD,)-313.4(Ramirez)-321.8(JA,)-316.2(Summersgill)]TJ 2.5165 -1.1128 TD [(JT)72.2(.)-224.2(Use)-230.4(of)-227.6(heteroduplex)-226.6(analysis)-230.2(to)-229(classify)-231.2(legionellae)-230.6(on)]TJ 0 -1.1192 TD [(the)-348.4(basis)-343.4(of)-347.7(5S)-345.3(rRNA)-351.7(gene)-345.3(sequences.)]TJ /F8 1 Tf 16.0539 0 TD [(J)-347(Clin)-348.9(Micr)29.8(obiol)]TJ /F4 1 Tf -16.0539 -1.1128 TD [(1997;)-334.9(35:)-329.6(1609\261161)29.4(1.)]TJ -2.5165 -1.1128 TD [(72.)-1266.5(Skot)-365.7(J,)-370.3(Lerche)-367.4(AG,)-368.9(Kolmos)-372.4(HJ,)-366.8(Nielsen)-372(JO,)-366.8(Mathiesen)]TJ 2.5165 -1.1128 TD [(LR,)-414.8(Lundgren)-417.5(JD.)-417.4(Pneumocystis)-424.7(carinii)-421.4(in)-412.4(bronchoalv-)]TJ 0 -1.1192 TD [(eolar)-265.9(lavage)-270.5(and)-270.1(induced)-263.9(sputum:)-269.8(detection)-273.8(with)-265.3(a)-271.2(nested)]TJ 0 -1.1128 TD [(polymerase)-335.7(chain)-334.5(reaction.)]TJ /F8 1 Tf 11.1283 0 TD [(Scand)-332.3(J)-334.4(Infect)-337.3(Dis)]TJ /F4 1 Tf 7.8214 0 TD [(1995;)-328.6(27:)]TJ -18.9498 -1.1192 TD (363\261367.)Tj -2.5165 -1.1065 TD [(73.)-1266.5(Roth)-384.4(A,)-391.4(Schaber)11(g)-385.2(T)72.9(,)-388.6(Mauch)-389.7(H.)-385.1(Molecular)-393.2(diagnosis)-388(of)]TJ 2.5165 -1.1192 TD [(tuberculosis:)-328.3(current)-322.8(clinical)-327.2(validity)-321.4(and)-320.7(future)-323.5(perspec-)]TJ 0 -1.1128 TD (tives.)Tj /F8 1 Tf 2.447 0 TD [(Eur)-331.9(Respir)-333.8(J)]TJ /F4 1 Tf 5.5073 0 TD [(1997;)-334.9(10:)-336(1877\2611891.)]TJ -10.4707 -1.1128 TD [(74.)-1266.5(Crawford)-316.4(SW)79(,)-312.7(Bowden)-320.9(RA,)-313.4(Hackman)-317.1(RC,)-321.1(Gleaves)-312.4(CA,)]TJ 2.5165 -1.1191 TD [(Meyers)-220.6(JD,)-215(Clark)-220.3(JG.)-221.4(Rapid)-219.3(detection)-216.9(of)-221.3(cytomegalovirus)]TJ 0 -1.1128 TD [(pulmonary)-714.9(infection)-711.2(by)-707.2(bronchoalveolar)-718.9(lavage)-713.1(and)]TJ 0 -1.1192 TD [(centrifugation)-357.7(culture.)]TJ /F8 1 Tf 9.2378 0 TD [(Ann)-349.7(Intern)-355.4(Med)]TJ /F4 1 Tf 6.8351 0 TD [(1988;)-353.9(108:)-354.4(180\261)]TJ -16.0728 -1.1128 TD (185.)Tj -2.5165 -1.1128 TD [(75.)-1266.5(Eber)-362.6(E.)-366.3(Infection)-362.1(control)-368.7(in)-361.8(flexible)-370.3(fiberoptic)-363.9(broncho-)]TJ 2.5165 -1.1128 TD [(scopy)64.8(.)]TJ /F8 1 Tf 2.82 0 TD (In)Tj /F4 1 Tf 0.822 0 TD [(:)-305.4(Barbato)-313.9(A,)-309.2(Landau)-309.8(LI,)-309.8(Scheinmann)-313.2(P)109.4(,)-312.7(W)70.8(arner)]TJ -3.642 -1.1192 TD [(JO,)-240.3(Zach)-241.3(M,)-243.4(eds.)-242.6(The)-235.7(bronchoscope)-241.9(\261)-246.1(flexible)-243.9(and)-238.5(rigid)-243.5(\261)]TJ 0 -1.1128 TD [(in)-330.2(children.)-339.2(T)28.6(reviso,)-333(Italy)60.3(,)-331.7(Arcari,)-335.8(1995;)-334.9(pp.)-330.7(87\26192.)]TJ -2.5165 -1.1128 TD [(76.)-1266.5(T)60.2(essier)-337.3(V)123.6(,)-338(Chadelat)-339.8(K,)-334.5(Baculard)-344.7(A,)-334.5(Housset)-339.4(B,)-342.2(Clement)]TJ 2.5165 -1.1192 TD [(A.)-353.5(A)-356.9(controlled)-363(study)-355.1(of)-354(differential)-353.4(cytology)-354.5(and)-358.7(cyto-)]TJ 0 -1.1128 TD [(kine)-524.9(expression)-525(profiles)-528.4(by)-523.8(alveolar)-525.8(cells)-527.8(in)-526.2(pediatric)]TJ 0 -1.1192 TD (sarcoidosis.)Tj /F8 1 Tf 4.9761 0 TD (Chest)Tj /F4 1 Tf 2.5924 0 TD [(1996;)-334.9(109:)-335.5(1430\2611438.)]TJ -10.085 -1.1065 TD [(77.)-1266.5(Clement)-374.1(A,)-372.4(Chadelat)-377.8(K,)-366.1(Massliah)-376.4(J,)]TJ /F8 1 Tf 17.7611 0 TD [(et)-374.2(al)]TJ /F4 1 Tf 1.8526 0 TD [(.)-369.7(A)-375.9(controlled)]TJ -17.0971 -1.1192 TD [(study)-418.4(of)-417.3(oxygen)-420.4(metabolise)-421.7(release)-423.7(by)-416.3(alveolar)-424.6(macro-)]TJ 0 -1.1128 TD [(phages)-427.6(from)-425.4(children)-424.8(with)-423.3(interstitial)-435.9(lung)-424(disease.)]TJ /F8 1 Tf 21.4979 0 TD (Am)Tj -21.4979 -1.1192 TD [(Rev)-330.3(Respir)-340.1(Dis)]TJ /F4 1 Tf 6.4367 0 TD [(1987;)-328.6(136:)-335.5(1424\2611428.)]TJ -8.9532 -1.1128 TD [(78.)-1266.5(Heaney)-341(G,)-334.5(Stevenson)-338.6(EC,)-338.9(T)28.6(urner)-334.3(G,)]TJ /F8 1 Tf 17.7674 0 TD [(et)-336.3(al)]TJ /F4 1 Tf 1.8147 0 TD [(.)-338(Investigating)]TJ -17.0655 -1.1128 TD [(paediateic)-298.1(airways)-301(by)-296.2(non-bronchoscopic)-299.6(lavage:)-297.7(normal)]TJ 0 -1.1192 TD [(cellular)-338.5(data.)]TJ /F8 1 Tf 5.5073 0 TD [(Clin)-336.3(Exp)-330.5(Aller)24.9(gy)]TJ /F4 1 Tf 7.1259 0 TD [(1996;)-328.6(26:)-336(799\261806.)]TJ -15.1497 -1.1065 TD [(79.)-1266.5(Merchant)-385(RK,)-376.6(Schwartz)-386.2(DA,)-375.2(Helmers)-385.1(RA,)-382.9(Dayton)-381.8(CS,)]TJ 2.5165 -1.1192 TD [(Hunninghake)-568.7(GW)80.6(.)-565.7(Bronchoalveolar)-571.9(lavage)-574(cellularity)56.6(.)]TJ 0 -1.1128 TD [(The)-248.3(distribution)-260.9(in)-248(normal)-254.8(volunteers.)]TJ /F8 1 Tf 15.3078 0 TD [(Am)-252.4(Rev)-248.1(Respir)-257.9(Dis)]TJ /F4 1 Tf -15.3078 -1.1192 TD [(1992;)-334.9(146:)-329.1(448\261453.)]TJ -2.5165 -1.1065 TD [(80.)-1266.5(Braun)-413.9(J,)-414.6(Dalhof)-414.3(K,)-410.4(Ratjen)-417(F)71.5(.)-413.9(Differe)8.1(nt)-412.4(protein)-419.3(compo-)]TJ 2.5165 -1.1192 TD [(sition)-308.8(of)-303.5(BALF)-311.5(in)-304.9(healthy)-310.6(children)-304.7(and)-308.1(adults.)-309.1(T)60.2(echnical)]TJ 0 -1.1128 TD [(and)-333.4(clinical)-333.6(implications.)]TJ /F8 1 Tf 10.4644 0 TD [(Eur)-332(Respir)-340.1(J)]TJ /F4 1 Tf 5.5073 0 TD [(1994;)-334.9(7:)-336.5(228A.)]TJ -18.4882 -1.1128 TD [(81.)-1266.5(Bjermer)-278.8(L,)-277.8(Rust)-271.8(M,)-275(Heurlin)-283.4(N,)-271.3(Rennard)-279.9(S,)-272.9(Klech)-280.9(H.)-271.3(The)]TJ 2.5165 -1.1192 TD [(clinical)-453.7(use)-454.7(of)-448.9(bronchoalveolar)-453.4(lavage)-453.9(in)-450.3(patients)-459.1(with)]TJ 0 -1.1128 TD [(pulmonary)-297.6(infections.)]TJ /F8 1 Tf 9.0354 0 TD [(Eur)-294(Respir)-295.8(Rev)]TJ /F4 1 Tf 6.4367 0 TD [(1992;)-290.7(2:)-298.5(106\2611)34.1(13.)]TJ -17.9887 -1.1128 TD [(82.)-1266.5(Ratjen)-486.5(F)71.5(,)-483.5(KreuzLelder)-491.4(E.)-480.1(Differences)-483.8(in)-481.9(immunoglob-)]TJ 2.5165 -1.1128 TD [(ulines)-470.6(and)-472.5(B2-microglobulin)-477.5(levels)-470.4(of)-474.2(bronchoalveolar)]TJ 0 -1.1192 TD [(lavage)-422.3(fluid)-420.5(between)-418.7(normal)-419.2(children)-418.5(and)-421.9(adults.)]TJ /F8 1 Tf 20.7708 0 TD (Lung)Tj /F4 1 Tf -20.7708 -1.1128 TD [(1996;)-334.9(174:)-329.1(383\261391.)]TJ -2.5165 -1.1128 TD [(83.)-1266.5(Ratjen)-233.6(F)71.5(,)-230.5(Rehn)-230.1(B,)-234.7(Costabel)-233.8(U,)-233.3(Brunch)-230(J.)-231.2(Age)-235.5(dependency)]TJ 2.5165 -1.1192 TD [(of)-474.2(surfactant)-476.3(phospholipid)-470.1(and)-472.5(surfactant)-476.3(protein)-476.2(A)-470.7(i)0(n)]TJ 0 -1.1128 TD [(bronchoalveolar)-421.8(lavage)-416(fluid)-420.5(children)-418.5(without)-417.9(broncho-)]TJ 0 -1.1192 TD [(pulmonary)-335.5(disease.)]TJ /F8 1 Tf 8.068 0 TD [(Eur)-338.3(Respir)-333.8(J)]TJ /F4 1 Tf 5.5136 0 TD [(1996;)-328.6(9:)-336.5(328\261333.)]TJ 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-1.1002 -1.1128 TD [(de)-333.9(Blic)-336(J.)-332.4(Cd)-338.8(la-positive)-340.7(cells)-331.8(in)-336.5(bronchoalveolar)-339.6(lavage)]TJ 0 -1.1192 TD [(samples)-249.2(from)-248.4(children)-247.8(with)-246.3(Langerhans)-252.8(cell)-248.9(histiocytosis.)]TJ /F8 1 Tf 0 -1.1128 TD [(J)-328.1(Pediatr)]TJ /F4 1 Tf 4.0656 0 TD [(1996;)-334.9(129:)-335.5(913\261915.)]TJ -6.5821 -1.1128 TD [(91.)-1266.5(Conference)-538.1(C.)-531.9(Activity)-539.7(of)-537.4(sarcoidosis:)-536.2(third)-540.6(W)108.7(ASOG)]TJ 2.5165 -1.1128 TD (meeting.)Tj /F8 1 Tf 3.7685 0 TD [(Eur)-331.9(Respir)-333.8(J)]TJ /F4 1 Tf 5.5073 0 TD [(1994;)-334.9(7:)-330.2(624\261627.)]TJ -11.7922 -1.1128 TD [(92.)-1266.5(Fireman)-240.1(E,)-233.5(T)60.2(opilsky)-242.7(M.)-237(Sarcoidosis:)-242.8(an)-239(or)12.7(ganized)-238.4(pattern)]TJ 2.5165 -1.1128 TD [(of)-278.2(reaction)-285.5(from)-280(immunology)-281.6(to)-279.6(therapy)57.6(.)]TJ /F8 1 Tf 16.604 0 TD [(Immunol)-278.2(T)84.1(oday)]TJ /F4 1 Tf -16.604 -1.1191 TD [(1994;)-328.6(15:)-336(199\261201.)]TJ -2.5165 -1.1128 TD [(93.)-1266.5(Chadelat)-390.4(K,)-391.4(Baculard)-395.3(A,)-391.4(Grimfeld)-393.3(A,)]TJ /F8 1 Tf 18.4692 0 TD [(et)-393.2(al)]TJ /F4 1 Tf 1.8716 0 TD [(.)-388.6(Pulmonary)]TJ -17.8243 -1.1128 TD [(sarcoidosis)-382.6(in)-380.8(children:)-382.4(serial)-382.8(evaluation)-387.1(of)-379.3(bronchoalv-)]TJ 0 -1.1192 TD [(eolar)-253.3(lavage)-251.6(cells)-255.9(during)-253.2(corticosteroid)-257.7(treatment.)]TJ /F8 1 Tf 19.8476 0 TD (Pediatr)Tj -19.8476 -1.1128 TD (Pulmonol)Tj /F4 1 Tf 4.1921 0 TD [(1993;)-334.9(316:)-335.5(41\26147.)]TJ -6.7086 -1.1128 TD [(94.)-1266.5(Peters)-720.4(MJ,)-724.6(Klein)-719.8(NJ,)-727.2(Gibb)-718.1(DM.)-726.7(HIV)82.5(-related)-722.8(lung)]TJ 2.5165 -1.1128 TD [(disorders)-349.6(in)-355.5(infants)-350.9(and)-352.3(children,)-351.9(including)-356.4(lymphocytic)]TJ 0 -1.1192 TD [(interstitial)-398(pneumonitis.)]TJ /F8 1 Tf 9.9143 0 TD [(Eur)-395.2(Respir)-397(Mon)]TJ /F4 1 Tf 7.0753 0 TD [(1995;)-391.9(2:)-393.4(362\261)]TJ -16.9897 -1.1128 TD (384.)Tj -2.5165 -1.1128 TD [(95.)-1266.5(Leigh)-635.4(MW)83.2(,)-635.2(Henshaw)-642.4(NG,)-634.4(W)70.8(ood)-637.1(RE.)-636.1(Diagnosis)-644.2(of)]TJ /F8 1 Tf 2.5165 -1.1192 TD [(Pneumcystis)-228.9(carinii)]TJ /F4 1 Tf 8.0364 0 TD [(pneumonia)-233.2(in)-229(pediatric)-236.8(patients)-231.4(using)]TJ -8.0364 -1.1128 TD [(bronchoscopic)-237.5(bronchoalveolar)-238.4(lavage.)]TJ /F8 1 Tf 15.763 0 TD [(Pediatr)-239.6(Infect)-236.2(Dis)]TJ /F4 1 Tf -15.763 -1.1191 TD [(1985;)-328.6(4:)-336.5(408\261410.)]TJ -2.5165 -1.1065 TD [(96.)-1266.5(Bye)-249.6(MR,)-260.2(Bernstein)-259.5(L,)-252.5(Shah)-255.6(K,)-252.3(Ellaurie)-260(M,)-256(Rubinstein)-260.6(A.)]TJ 2.5165 -1.1192 TD [(Diagnostic)-745.9(bronchoalveolar)-756.9(lavage)-744.8(in)-753.8(children)-747.3(with)]TJ 0 -1.1128 TD (AIDS.)Tj /F8 1 Tf 2.8896 0 TD [(Pediatr)-334.4(Pulmonol)]TJ /F4 1 Tf 7.499 0 TD [(1987;)-334.9(3:)-336.5(425\261428.)]TJ -12.9051 -1.1128 TD [(97.)-1266.5(Mattey)-754.4(JE,)-759(Fitzpatrick)-761.5(SB,)-757.6(Josephs)-758.5(SH,)-762.5(Fink)-757.7(RJ.)]TJ 2.5165 -1.1192 TD [(Bronchoalveolar)-369.6(lavage)-371.7(for)-373.5(pneumocystis)-369.2(pneumonia)-372.3(in)]TJ 0 -1.1128 TD [(HIV)82.5(-infected)-336.5(children.)]TJ /F8 1 Tf 9.3263 0 TD [(Ann)-337.1(Aller)31.3(gy)]TJ /F4 1 Tf 5.1468 0 TD [(1990;)-334.9(64:)-329.7(393\261397.)]TJ -16.9897 -1.1128 TD [(98.)-1266.5(Birriel)-465.2(JA)-465.1(Jr)30.5(,)-464.5(Adams)-471.5(JA,)-461.6(Saldana)-464.6(MA,)]TJ /F8 1 Tf 19.5125 0 TD [(et)-469(al)]TJ /F4 1 Tf 1.9475 0 TD [(.)-464.5(Role)-460.1(of)]TJ -18.9434 -1.1128 TD [(flexible)-300.8(bronchoscopy)-299.1(and)-301.8(bronchoalveolar)-301.6(lavage)-295.8(in)-304.9(the)]TJ 0 -1.1192 TD [(diagnosis)-451.3(of)-455.2(pediatric)-451.8(acquired)-460.2(immunodeficiency)-458(syn-)]TJ 0 -1.1128 TD [(drome-related)-457.2(pulmonary)-462(disease.)]TJ /F8 1 Tf 14.3024 0 TD (Pediatrics)Tj /F4 1 Tf 4.5146 0 TD [(1991;)-461.4(87:)]TJ -18.817 -1.1192 TD (897\26189.)Tj -2.5165 -1.1128 TD [(99.)-1266.5(Glaser)-611.3(JH,)-613.4(Schuval)-616.6(S,)-614.3(Burstein)-614.3(O,)-619(Bye)-610(MR.)-614.3(Cyto-)]TJ 2.5165 -1.1128 TD [(megalovirus)-236.7(and)]TJ /F8 1 Tf 6.8035 0 TD [(Pneumocystis)-241.1(carinii)]TJ /F4 1 Tf 8.5549 0 TD [(pneumonia)-233.2(in)-241.7(chil-)]TJ -15.3583 -1.1128 TD [(dren)-770.1(with)-777.4(acquired)-776.3(immunodeficiency)-774.2(syndrome.)]TJ /F8 1 Tf 22.3768 0 TD (J)Tj -22.3768 -1.1192 TD (Pediatr)Tj /F4 1 Tf 3.3006 0 TD [(1992;)-328.6(120:)-335.5(929\261931.)]TJ -5.8171 -1.1128 TD [(100.)-766.4(Allen)-214(KA,)-217.1(Markin)-222.1(RS,)-213.8(Rennard)-216.6(SI,)]TJ /F8 1 Tf 16.5723 0 TD [(et)-216.1(al)]TJ /F4 1 Tf 1.6945 0 TD [(.)-211.6(Bronchoalveolar)]TJ -15.7504 -1.1128 TD [(lavage)-365.4(in)-368.1(liver)-376(transplant)-370.5(patients.)]TJ /F8 1 Tf 14.2139 0 TD [(Acta)-370.3(Cyfol)]TJ /F4 1 Tf 4.6916 0 TD [(1989;)-372.9(33:)]TJ -18.9055 -1.1192 TD (539\261543.)Tj -2.5165 -1.1065 TD [(101.)-766.4(Colombo)-410.1(JL,)-411.2(Sammut)-412.4(PH,)-408.4(Langnas)-411.6(AN,)-413.1(Shaw)-410.6(BW)-407.7(Jr)49.4(.)]TJ 2.5165 -1.1191 TD [(The)-235.7(spectrum)-235.8(of)]TJ /F8 1 Tf 6.6896 0 TD [(Pneumocystis)-241.1(carinii)]TJ /F4 1 Tf 8.5549 0 TD [(infection)-237(after)-241.6(liver)]TJ -15.2445 -1.1128 TD [(transplantation)-562.2(in)-557.8(children.)]TJ /F8 1 Tf 11.8049 0 TD [(T)52.5(ransplantation)]TJ /F4 1 Tf 6.9109 0 TD [(1992;)-562.6(54:)]TJ -18.7158 -1.1192 TD (621\261624.)Tj -2.5165 -1.1128 TD [(102.)-766.4(Frankel)-408.9(LR,)-408.4(Smith)-407.3(DW)80.6(,)-407.6(Lewiston)-413.1(NJ.)-404.7(Bronchoalveolar)]TJ 2.5165 -1.1128 TD [(lavage)-207.3(for)-209.1(diagnosis)-211(of)-208.6(pneumonia)-214.2(in)-203.7(the)-209.3(immunocompro-)]TJ 0 -1.1192 TD [(mised)-331.5(child.)]TJ /F8 1 Tf 5.2543 0 TD (Pediatrics)Tj /F4 1 Tf 4.3944 0 TD [(1988;)-334.9(81:)-336(785\261788.)]TJ -12.1653 -1.1065 TD [(103.)-766.4(Pattishall)-783.8(EN,)-780.1(Noyes)-779.4(BE,)-781.5(Orenstein)-782.9(DM.)-777.3(Use)-786.8(of)]TJ 2.5165 -1.1192 TD [(bronchoalveolar)-630.4(lavage)-630.9(in)-633.7(immunocompromised)-637.6(chil-)]TJ 0 -1.1128 TD (dren.)Tj /F8 1 Tf 2.3395 0 TD [(Pediatr)-334.4(Pulmonol)]TJ /F4 1 Tf 7.499 0 TD [(1988;)-334.9(5:)-330.2(1\2615.)]TJ -12.355 -1.1128 TD [(104.)-766.4(W)32.8(inthrop)-267.7(AL,)-267.9(W)70.8(addell)-273.1(T)72.9(,)-268.5(Superina)-269.3(RA.)-269.1(The)-267.3(diagnosis)-274.2(of)]TJ 2.5165 -1.1192 TD [(pneumonia)-555.6(in)-551.5(the)-557.1(immunocompromised)-555.4(child:)-560.3(use)-555.9(of)]TJ 0 -1.1128 TD [(bronchoalveolar)-396.5(lavage.)]TJ /F8 1 Tf 10.0534 0 TD [(J)-391.3(Pediatr)-397.7(Sur)31.9(g)]TJ /F4 1 Tf 6.4367 0 TD [(1990;)-391.9(25:)-392.9(878\261)]TJ -16.4902 -1.1192 TD (880.)Tj -2.5165 -1.1065 TD [(105.)-766.4(Ratjen)-442.3(F)71.5(,)-445.5(Costabel)-448.7(U,)-448.3(Havers)-444.5(W)83.4(.)-445.5(Differential)-445.2(count)-448.5(of)]TJ 2.5165 -1.1191 TD [(bronchoalveolar)-339.6(lavage)-340.1(fluid)-344.6(in)-342.8(immunosuppressed)-343.5(chil-)]TJ 0 -1.1128 TD [(dren)-245.3(with)-246.3(pulmonary)-247(infiltrates.)]TJ /F8 1 Tf 12.8102 0 TD [(Ar)27.9(ch)-245.4(Dis)-251.9(Child)]TJ /F4 1 Tf 6.2217 0 TD [(1996;)-246.4(74:)]TJ -19.032 -1.1192 TD [(507\26151)34.7(1.)]TJ -2.5165 -1.1128 TD [(106.)-766.4(Lanino)-299.8(E,)-309.4(Sacco)-305.2(O,)-302.9(Kotitsa)-311.3(Z,)]TJ /F8 1 Tf 15.0485 0 TD [(et)-304.6(al)]TJ /F4 1 Tf 1.7831 0 TD [(.)-300.1(Fiberoptic)-311(broncho-)]TJ -14.3151 -1.1128 TD [(scopy)-365.2(and)-365(Bronchoalveolar)-375.9(lavage)-371.7(for)-367.1(the)-367.4(evaluation)-374.4(of)]TJ 0 -1.1192 TD [(pulmonary)-759.2(infiltrates)-761.4(after)-760.1(BMT)-728.2(in)-753.8(children.)]TJ /F8 1 Tf 20.7771 0 TD (Bone)Tj -20.7771 -1.1128 TD [(Marr)30.8(ow)-332.5(T)52.5(ransplantation)]TJ /F4 1 Tf 10.2431 0 TD [(1996;)-334.9(18:)-336(1)32.1(17\261120.)]TJ 15.2382 73.5671 TD [(107.)-766.4(Fauroux)-368.8(B,)-361.2(Meyer-Milsztain)-365.9(A,)-366.1(Boccon-Gibod)-371.5(L,)]TJ /F8 1 Tf 23.243 0 TD [(et)-367.9(al)]TJ /F4 1 Tf 1.8463 0 TD (.)Tj -22.5728 -1.1381 TD [(Cytotoxic)-456(Drung-induced)-462.1(pulmonary)-455.7(disease)-462.1(in)-456.6(infants)]TJ T* [(and)-333.4(children.)]TJ /F8 1 Tf 5.5895 0 TD [(Pediatr)-334.4(Pulmonol)]TJ /F4 1 Tf 7.499 0 TD [(1994;)-334.9(18:)-336(347\261355.)]TJ -15.6049 -1.1128 TD [(108.)-766.4(Prober)-433.5(CG,)-427.2(White)-431.2(H,)-429.3(Smith)-432.6(CR.)-428.6(Open)-431(lung)-430.3(biopsy)-430.5(in)]TJ 2.5165 -1.1381 TD [(immunocompromised)-460.5(children)-456.5(with)-461.3(pulmonary)-455.7(infiltra-)]TJ T* (tes.)Tj /F8 1 Tf 1.6756 0 TD [(Am)-334.6(J)-334.4(Dis)-334.1(Child)]TJ /F4 1 Tf 6.677 0 TD [(1984;)-328.6(138:)-335.5(60\26163.)]TJ -10.8691 -1.1128 TD [(109.)-766.4(Shorter)-302.6(NA,)-305.7(Ross)-302.2(AJ,)-303.6(August)-301(C.)-304.3(The)-305.2(usefulness)-304.9(of)-303.5(open)]TJ 2.5165 -1.1381 TD [(lung)-582.1(biopsy)-582.2(in)-576.8(the)-582.3(pediatric)-584.6(bone)-579.5(marrow)-585.5(transplant)]TJ T* (population.)Tj /F8 1 Tf 4.8307 0 TD [(J)-334.4(Pediatr)-334.4(Sur)31.9(g)]TJ /F4 1 Tf 6.2597 0 TD [(1988;)-335(23:)-329.6(533\261537.)]TJ -13.6069 -1.1128 TD [(1)25.8(10.)-792.3(Guilinger)-727.4(RA,)-718.1(Paradis)-726.2(IL,)-720.8(Dauber)-721.6(JH,)]TJ /F8 1 Tf 20.6253 0 TD [(et)-722(al)]TJ /F4 1 Tf 2.2004 0 TD [(.)-717.4(The)]TJ -20.3092 -1.1381 TD [(importance)-381.2(of)-379.3(bronchoscopy)-381.3(with)-385.4(transbronchial)-381.8(biopsy)]TJ T* [(and)-390.3(bronchoalveolar)-396.5(lavage)-390.7(in)-387.1(the)-392.7(management)-392.1(of)-392(lung)]TJ T* [(transplant)-370.5(recipients.)]TJ /F8 1 Tf 8.7066 0 TD [(Am)-366.2(J)-366(Respir)-365.4(Crit)-369(Car)33.6(e)-366(Med)]TJ /F4 1 Tf 11.8428 0 TD (1995;)Tj -20.5495 -1.1381 TD [(152:)-335.5(2037\2612043.)]TJ -2.5165 -1.1128 TD [(1)25.8(1)32.1(1.)-824.4(Magnan)-338.6(A,)-334.5(Mege)-333.3(J-L,)-342.1(Reynaud)-335.8(M,)]TJ /F8 1 Tf 17.2552 0 TD [(et)-336.3(al)]TJ /F4 1 Tf 1.8147 0 TD [(.)-338(Monitoring)-338.8(of)]TJ -16.5534 -1.1381 TD [(alveolar)-247.6(macrophage)-249.7(production)-247(of)-246.6(tumor)-243(necrosis)-247.8(factor-)]TJ T* [(alpha)-233.3(and)-232.2(interleukin-6)-238.3(in)-229(lung)-234.3(transplant)-237.7(recipients.)]TJ /F8 1 Tf 20.8277 0 TD [(Am)-233.4(J)]TJ -20.8277 -1.1381 TD [(Respir)-333.8(Crit)-337.4(Car)33.6(e)-334.4(Med)]TJ /F4 1 Tf 9.2251 0 TD [(1994;)-334.9(150:)-335.5(684\261689.)]TJ -11.7416 -1.1128 TD [(1)25.8(12.)-792.3(Clelland)-418.3(C,)-411.8(Higenbottam)-417.3(T)72.9(,)-413.9(Stewart)-414.6(S,)]TJ /F8 1 Tf 19.032 0 TD [(et)-412.1(al)]TJ /F4 1 Tf 1.8906 0 TD [(.)-413.9(Broncho-)]TJ -18.406 -1.1381 TD [(alveolar)-494.2(lavage)-491.8(and)-491.4(transbronchial)-495.6(lung)-493.5(biopsy)-493.7(during)]TJ T* [(acute)-606.2(rejection)-603.5(and)-605.3(infection)-603.8(in)-602.1(heart-lung)-608.1(transplant)]TJ T* 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